EC: 3.1.2.2

palmitoyl-CoA hydrolase (palmitoyl-CoA hydrolase)

enzyme lambda metabolic reaction experiment
uniprot:Q91V12 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Palmitoyl_CoA + H2O -> Coenzyme_A + Palmitate		 (( (Vmax * A) ) / (Km + A)) buffer:
PH: 7
Temperature: 30
uniprot:Q91V12 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

H2O + Palmitoyl_CoA -> Coenzyme_A + Palmitate		 (( (Vmax * A) ) / (Km + A)) buffer:
PH: 7
Temperature: 30
uniprot:O00154 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Palmitoyl_CoA + H2O -> Coenzyme_A + Palmitate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 30
uniprot:O00154 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Oleoyl_CoA + H2O -> Coenzyme_A + Oleate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 30
uniprot:O00154 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Eicosanoyl_CoA + H2O -> Arachidic_acid + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 30
uniprot:O00154 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Lauroyl_CoA + H2O -> Dodecanoate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 30
uniprot:O00154 [palmitoyl-CoA hydrolase(Enzyme) wildtype BACH]

Octanoyl_CoA + H2O -> Octanoate + Coenzyme_A		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Sodium phosphate, 0.1 mM 5,5'-Dithiobis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 30
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

Linoleoyl_CoA + H2O -> Coenzyme_A + Linoleate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

H2O + Arachidonyl_CoA -> Arachidonate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

alpha_Linolenoyl_CoA + H2O -> Coenzyme_A + alpha_Linolenate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

H2O + Palmitoyl_CoA -> Coenzyme_A + Palmitate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

Oleoyl_CoA + H2O -> Oleate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

Stearoyl_CoA + H2O -> Stearate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

H2O + Myristoyl_CoA -> Coenzyme_A + Myristic_acid		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q8N1Q8 [palmitoyl-CoA hydrolase(Enzyme) wildtype delta34Them5]

Palmitoleoyl_CoA + H2O -> Palmitoleic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM HEPES, 50 mM KCl, 0.5 mM DTNB
PH: 7.5
Temperature: 37
uniprot:O14734 [palmitoyl-CoA hydrolase(Enzyme) wildtype]

Decanoyl_CoA + H2O -> Coenzyme_A + Decanoate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M potassium phosphate, 0.1 mM 5,5'-dithiobis(2-nitrobenzoate), 20 mg/ml bovine serum albumin
PH: 8
Temperature: 22
uniprot:Q5T1C6 [Undecan-2-one-CoA]

H2O + Decanoyl_CoA -> Coenzyme_A + Decanoate		 (( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant H152F His6-tagged]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant R206A His6-tagged]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant T177A His6-tagged]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant H152A His6-tagged]

Myristoyl_CoA + H2O -> Coenzyme_A + Myristic_acid		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant D161N His6-tagged]

Myristoyl_CoA + H2O -> Coenzyme_A + Myristic_acid		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant N183A His6-tagged]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant K207A His6-tagged]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant D161E His6-tagged]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) wildtype His6-tagged]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM K+HEPES, 0.2 M KCl, 2 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q8GYW7 [Bovine serum albumin]

Oleoyl_CoA + H2O -> Coenzyme_A + Oleate		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (( (S ^ h) ) * (1 + (S / Ks))))) buffer: 50 mM Potassium phosphate, 200 µM 5,5'-Dithio-bis(2-nitrobenzoic acid)
PH: 8
Temperature: 36
uniprot:Q8GYW7 [palmitoyl-CoA hydrolase(Enzyme) wildtype ACH2 6-His-tagged]

Lauroyl_CoA + H2O -> Dodecanoate + Coenzyme_A		 (( (Vmax * (S ^ h)) ) / (( (S_h ^ h) ) + (( (S ^ h) ) * (1 + (S / Ks))))) buffer: 50 mM Potassium phosphate, 200 µM 5,5'-Dithio-bis(2-nitrobenzoic acid)
PH: 8
Temperature: 36
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Palmitoyl_CoA -> Coenzyme_A + Palmitate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Myristoyl_CoA -> Coenzyme_A + Myristic_acid		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Decanoyl_CoA -> Decanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Octanoyl_CoA -> Octanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Palmitoleoyl_CoA -> Coenzyme_A + Palmitoleic_acid		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Stearoyl_CoA -> Coenzyme_A + Stearate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 5
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 7.2
Temperature: 20
uniprot:Q64559 [palmitoyl-CoA hydrolase(Enzyme) wildtype isoenzyme CTE-II]

H2O + Palmitoyl_CoA -> Coenzyme_A + Palmitate		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Hepes, 50 mM KCl, 0.025% Triton X-100, 0.3 mM DTNB
PH: 7.4
Temperature: 25
- [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + Palmitoyl_CoA -> Palmitate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 10 mM Hepes, 50 mM KCl, 0.025% Triton X-100, 0.3 mM DTNB
PH: 7.4
Temperature: 25
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant G44A]

Lauroyl_CoA + H2O -> Dodecanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 mM DTNB, 80 µg/ml BSA, 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant D154A]

H2O + Lauroyl_CoA -> Coenzyme_A + Dodecanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 mM DTNB, 80 µg/ml BSA, 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant N73A]

Lauroyl_CoA + H2O -> Coenzyme_A + Dodecanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 mM DTNB, 80 µg/ml BSA, 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant S10A]

Lauroyl_CoA + H2O -> Dodecanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 mM DTNB, 80 µg/ml BSA, 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + N_Carbobenzoxy_L_tyrosine_p_nitrophenyl_ester -> p_Nitrophenol + N_Carbobenzoxy_L_tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant N73A]

H2O + N_Carbobenzoxy_L_tyrosine_p_nitrophenyl_ester -> p_Nitrophenol + N_Carbobenzoxy_L_tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant G44A]

N_Carbobenzoxy_L_tyrosine_p_nitrophenyl_ester + H2O -> p_Nitrophenol + N_Carbobenzoxy_L_tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant D154A]

H2O + N_Carbobenzoxy_L_tyrosine_p_nitrophenyl_ester -> N_Carbobenzoxy_L_tyrosine + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant S10A]

N_Carbobenzoxy_L_tyrosine_p_nitrophenyl_ester + H2O -> p_Nitrophenol + N_Carbobenzoxy_L_tyrosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 M sodium phosphate
PH: 7.5
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) wildtype]

Lauroyl_CoA + H2O -> Coenzyme_A + Dodecanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 mM DTNB, 80 µg/ml BSA, 50 mM potassium phosphate
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant G44A]

H2O + p_Nitrophenyl_butyrate -> p_Nitrophenol + Butanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant D154A]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant H157A]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) wildtype]

H2O + p_Nitrophenyl_butyrate -> Butanoate + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant N73A]

H2O + p_Nitrophenyl_butyrate -> p_Nitrophenol + Butanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:P0ADA1 [palmitoyl-CoA hydrolase(Enzyme) mutant S10A]

p_Nitrophenyl_butyrate + H2O -> Butanoate + p_Nitrophenol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM sodium phosphate, 21% (v/v) Triton X-100
PH: 7
Temperature: 37
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + 4_Hydroxybenzoyl_CoA -> Coenzyme_A + 4_Hydroxybenzoate		 (( (( (kcat * E) ) * A) ) / (A + (Km * (1 + (I / Ki))))) buffer: 50 mM K+HEPES, 0.2 M KCl
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Palmitoyl_CoA + H2O -> Coenzyme_A + Palmitate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + Oleoyl_CoA -> Coenzyme_A + Oleate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Arachidonyl_CoA + H2O -> Arachidonate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Lauroyl_CoA + H2O -> Dodecanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Decanoyl_CoA + H2O -> Coenzyme_A + Decanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Tetradecanoyl_[acp] + H2O -> Myristic_acid + Holo_[acyl_carrier_protein]		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 0.3 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + Acetyl_CoA -> Acetate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Succinyl_CoA + H2O -> Succinate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Octanoyl_CoA + H2O -> Octanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Hexanoyl_CoA + H2O -> Hexanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + Propanoyl_CoA -> Coenzyme_A + Propanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

2_Methylpropanoyl_CoA + H2O -> Coenzyme_A + Isobutanoate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Butanoyl_CoA + H2O -> Butanoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

3_Hydroxyphenylacetyl_CoA + H2O -> Coenzyme_A + 3_Hydroxyphenylacetate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + 2_Hydroxybenzoyl_CoA -> Salicylate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + 4_Hydroxybenzoyl_CoA -> 4_Hydroxybenzoate + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) wildtype N-terminal His6-tag]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

Myristoyl_CoA + H2O -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 37
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta99 N-terminal His6-MBP-tag]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25
uniprot:Q5T1C6 [palmitoyl-CoA hydrolase(Enzyme) mutant delta39 C-terminal His6-tag]

H2O + Myristoyl_CoA -> Myristic_acid + Coenzyme_A		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM K+HEPES, 0.2 M KCl, 1 mM DTNB
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-5996 oleate biosynthesis
BioCyc:HUMAN_PWY-5148 acyl-CoA hydrolysis
BioCyc:META_PWY-7741 phthiocerol biosynthesis
BioCyc:META_FAO-PWY fatty acid β-oxidation I
BioCyc:META_PWY-5996 oleate biosynthesis II (animals and fungi)
BioCyc:MOUSE_PWY-6061 bile acid biosynthesis, neutral pathway
BioCyc:MTBH37RV_PWY-7741 phthiocerol biosynthesis
BioCyc:META_PWY-5148 acyl-CoA hydrolysis
BioCyc:META_PWY-7292 oleate β-oxidation (thioesterase-dependent, yeast)
BioCyc:MTBH37RV_PWY-7742 phenolphthiocerol biosynthesis
BioCyc:HUMAN_LIPASYN-PWY phospholipases
BioCyc:HUMAN_PWY-5972 stearate biosynthesis
BioCyc:HUMAN_FAO-PWY fatty acid β-oxidation
BioCyc:META_PWY-5972 stearate biosynthesis I (animals and fungi)
BioCyc:YEAST_PWY-7292 oleate β-oxidation (thioesterase-dependent)
BioCyc:MOUSE_FAO-PWY fatty acid β-oxidation I
BioCyc:MOUSE_LIPASYN-PWY phospholipases
BioCyc:MOUSE_PWY-5148 acyl-CoA hydrolysis
BioCyc:MOUSE_PWY-5136 fatty acid β-oxidation II (core pathway)
BioCyc:ECOL316407_PWY-5148 acyl-CoA hydrolysis
PathBank:SMP0000817 Fatty Acid Metabolism
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-9918432 Maturation of DENV proteins
Reactome:R-HSA-9918487 Dengue Virus Genome Translation and Replication
Reactome:R-HSA-9839923 Dengue Virus Infection
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-HSA-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-HSA-194068 Bile acid and bile salt metabolism
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-2029480 Fcgamma receptor (FCGR) dependent phagocytosis
Reactome:R-MMU-168256 Immune System
Reactome:R-MMU-168249 Innate Immune System
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-75105 Fatty acyl-CoA biosynthesis
Reactome:R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-MMU-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-RNO-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-2029485 Role of phospholipids in phagocytosis
Reactome:R-SCE-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SCE-194068 Bile acid and bile salt metabolism
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-HSA-1482798 Acyl chain remodeling of CL
Reactome:R-HSA-2029485 Role of phospholipids in phagocytosis
Reactome:R-MMU-2029480 Fcgamma receptor (FCGR) dependent phagocytosis
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
Reactome:R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-RNO-390918 Peroxisomal lipid metabolism
Reactome:R-RNO-192105 Synthesis of bile acids and bile salts
Reactome:R-RNO-159418 Recycling of bile acids and salts
Reactome:R-RNO-168256 Immune System
Reactome:R-RNO-168249 Innate Immune System
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-8978868 Fatty acid metabolism
Reactome:R-SCE-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-SCE-390918 Peroxisomal lipid metabolism
Reactome:R-DME-8978868 Fatty acid metabolism
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-HSA-192105 Synthesis of bile acids and bile salts
Reactome:R-HSA-159418 Recycling of bile acids and salts
Reactome:R-HSA-168256 Immune System
Reactome:R-HSA-168249 Innate Immune System
Reactome:R-MMU-194068 Bile acid and bile salt metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-75105 Fatty acyl-CoA biosynthesis
Reactome:R-RNO-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-RNO-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-RNO-2029480 Fcgamma receptor (FCGR) dependent phagocytosis
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-SCE-192105 Synthesis of bile acids and bile salts
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-75105 Fatty acyl-CoA biosynthesis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-390918 Peroxisomal lipid metabolism
Reactome:R-HSA-1483206 Glycerophospholipid biosynthesis
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-2029485 Role of phospholipids in phagocytosis
Reactome:R-MMU-390918 Peroxisomal lipid metabolism
Reactome:R-MMU-192105 Synthesis of bile acids and bile salts
Reactome:R-MMU-159418 Recycling of bile acids and salts
Reactome:R-RNO-194068 Bile acid and bile salt metabolism
Reactome:R-SCE-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-SCE-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
WikiPathways:WP5064 7-oxo-C and 7-beta-HC pathways
WikiPathways:WP4545 Oxysterols derived from cholesterol
WikiPathways:WP5176 Disorders of bile acid synthesis and biliary transport
WikiPathways:WP2318 Fatty acid oxidation
PathBank:SMP0120662 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120806 3-Hydroxyisobutyric Aciduria
PathBank:SMP0120822 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0120826 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0120440 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120444 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120468 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0120516 Maple Syrup Urine Disease
PathBank:SMP0120589 Isovaleric Acidemia
PathBank:SMP0000032 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0000051 Fatty Acid Metabolism
PathBank:SMP0000173 beta-Ketothiolase Deficiency
PathBank:SMP0000199 Maple Syrup Urine Disease
PathBank:SMP0000384 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000235 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0000482 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0000318 Congenital Bile Acid Synthesis Defect Type III
PathBank:SMP0000141 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0000524 Isovaleric Acidemia
PathBank:SMP0000540 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0000544 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0000568 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0002324 Biosynthesis of Unsaturated Fatty Acids
PathBank:SMP0002346 Biosynthesis of Unsaturated Fatty Acids (Icosanoyl-CoA)
PathBank:SMP0087294 Fatty Acid Elongation in Mitochondria
PathBank:SMP0087358 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063612 Fatty Acid Elongation In Mitochondria
PathBank:SMP0120661 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0120665 3-Methylglutaconic Aciduria Type IV
PathBank:SMP0120745 Methylmalonic Aciduria
PathBank:SMP0120757 Propionic Acidemia
PathBank:SMP0120805 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0120825 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0120439 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120443 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120459 beta-Ketothiolase Deficiency
PathBank:SMP0120463 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0120467 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0120495 Zellweger Syndrome
PathBank:SMP0120503 Isovaleric Aciduria
PathBank:SMP0120523 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120588 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120608 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0120632 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0000054 Fatty Acid Elongation in Mitochondria
PathBank:SMP0000035 Bile Acid Biosynthesis
PathBank:SMP0000138 3-Hydroxy-3-methylglutaryl-CoA Lyase Deficiency
PathBank:SMP0000140 3-Methylglutaconic Aciduria Type III
PathBank:SMP0000238 Isovaleric Aciduria
PathBank:SMP0000185 Glutaric Aciduria Type I
PathBank:SMP0000481 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0000314 Congenital Bile Acid Synthesis Defect Type II
PathBank:SMP0000315 Cerebrotendinous Xanthomatosis (CTX)
PathBank:SMP0000523 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000539 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0002329 Biosynthesis of Unsaturated Fatty Acids (Docosanoyl-CoA)
PathBank:SMP0063641 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0063689 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0120652 27-Hydroxylase Deficiency
PathBank:SMP0120660 2-Methyl-3-hydroxybutryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120664 3-Methylglutaconic Aciduria Type III
PathBank:SMP0120696 Ethylmalonic Encephalopathy
PathBank:SMP0120736 Maple Syrup Urine Disease
PathBank:SMP0120740 Short-Chain Acyl-CoA Dehydrogenase Deficiency (SCAD Deficiency)
PathBank:SMP0120808 Isovaleric Acidemia
PathBank:SMP0120824 Very-Long-Chain Acyl-CoA Dehydrogenase Deficiency (VLCAD)
PathBank:SMP0120828 Trifunctional Protein Deficiency
PathBank:SMP0120441 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0120477 Familial Hypercholanemia (FHCA)
PathBank:SMP0120525 Methylmalonic Aciduria
PathBank:SMP0120537 Propionic Acidemia
PathBank:SMP0120586 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000137 2-Methyl-3-hydroxybutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0000237 3-Methylcrotonyl-CoA Carboxylase Deficiency Type I
PathBank:SMP0000200 Methylmalonic Aciduria
PathBank:SMP0000317 Familial Hypercholanemia (FHCA)
PathBank:SMP0000521 3-Hydroxyisobutyric Acid Dehydrogenase Deficiency
PathBank:SMP0000541 Carnitine Palmitoyl Transferase Deficiency II
PathBank:SMP0000545 Trifunctional Protein Deficiency
PathBank:SMP0002325 Biosynthesis of Unsaturated Fatty Acids (Tetracosanoyl-CoA)
PathBank:SMP0087280 Fatty Acid Metabolism
PathBank:SMP0087360 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids
PathBank:SMP0063640 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0120663 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120679 beta-Ketothiolase Deficiency
PathBank:SMP0120723 Isovaleric Aciduria
PathBank:SMP0120743 Methylmalonate Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120807 Isobutyryl-CoA Dehydrogenase Deficiency
PathBank:SMP0120823 Long Chain Acyl-CoA Dehydrogenase Deficiency (LCAD)
PathBank:SMP0120827 Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase Deficiency (LCHAD)
PathBank:SMP0120851 Short-Chain 3-Hydroxyacyl-CoA Dehydrogenase Deficiency (SCHAD)
PathBank:SMP0120442 3-Methylglutaconic Aciduria Type I
PathBank:SMP0120587 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000236 Propionic Acidemia
PathBank:SMP0000139 3-Methylglutaconic Aciduria Type I
PathBank:SMP0000181 Ethylmalonic Encephalopathy
PathBank:SMP0000480 Mitochondrial Beta-Oxidation of Short Chain Saturated Fatty Acids
PathBank:SMP0000316 Zellweger Syndrome
PathBank:SMP0000522 3-Hydroxyisobutyric Aciduria
PathBank:SMP0000538 Carnitine Palmitoyl Transferase Deficiency I
PathBank:SMP0000542 Medium Chain Acyl-CoA Dehydrogenase Deficiency (MCAD)
PathBank:SMP0000720 27-Hydroxylase Deficiency
PathBank:SMP0087327 Valine, Leucine, and Isoleucine Degradation
PathBank:SMP0087359 Mitochondrial Beta-Oxidation of Medium Chain Saturated Fatty Acids
PathBank:SMP0063601 Bile Acid Biosynthesis
PathBank:SMP0063639 Mitochondrial Beta-Oxidation of Long Chain Saturated Fatty Acids