EC: 5.3.3.2

isopentenyl-diphosphate DELTA-isomerase (3-methylbut-3-en-1-yl-diphosphate DELTA3-DELTA2-isomerase)

enzyme lambda metabolic reaction experiment
uniprot:Q46822 [Mg2+]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 200 mM KCl, 0.8 mM Dithiothreitol
PH: 7
Temperature: 37
uniprot:P61615 [isopentenyl-diphosphate Delta-isomerase(Enzyme) wildtype]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.05 M succinate-NaOH, 0.0025 M MgCl2, 9 mM sodium dithionite
PH: 6
Temperature: 60
uniprot:P58052 [Mg2+]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Hepes, 1 mM DTT
PH: 7
Temperature: 37
uniprot:Q9KWG2 [Mg2+]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Hepes, 1 mM DTT
PH: 7
Temperature: 37
uniprot:P58052 [Riboflavin-5-phosphate]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 HEPES, 5 mM MgCl2, 1 mM Dithithreitol, 5 mM NADPH, aerobic
PH: 7
Temperature: 37
uniprot:P58052 [Riboflavin-5-phosphate]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 HEPES, 5 mM MgCl2, 1 mM Dithithreitol, 10 mM Dithionite, aerobic
PH: 7
Temperature: 37
uniprot:P58052 [Riboflavin-5-phosphate]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 0.1 HEPES, 5 mM MgCl2, 1 mM Dithithreitol, 5 mM NADPH, anaerobic
PH: 7
Temperature: 37
uniprot:P58052 [Riboflavin-5-phosphate]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (( (E * kcat) ) * M) ) / (Km + M)) buffer: 0.1 HEPES, 5 mM MgCl2, 1 mM Dithithreitol, 5 mM NADPH, anaerobic
PH: 7
Temperature: 37
- [isopentenyl-diphosphate Delta-isomerase(Enzyme) wildtype]

Isopentenyl_diphosphate -> Dimethylallyl_diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Hepes, 0.8 mM DTT, 20 mM MgCl2
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6859 all-trans-farnesol biosynthesis
BioCyc:META_PWY-7102 bisabolene biosynthesis (engineered)
BioCyc:META_PWY-7392 taxadiene biosynthesis (engineered)
BioCyc:META_PWY-922 mevalonate pathway I
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
BioCyc:ECO_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:YEAST_HEXPPSYN-PWY-1 hexaprenyl diphosphate biosynthesis
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:GCF_000013425_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:META_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:ECO_POLYISOPRENSYN-PWY polyisoprenoid biosynthesis (E. coli)
BioCyc:YEAST_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:YEAST_PWY-922 mevalonate pathway
BioCyc:ARA_PWY-922 mevalonate pathway I
BioCyc:MTBH37RV_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:MTBH37RV_PWY-6383 mono-trans, poly-cis decaprenyl phosphate biosynthesis
BioCyc:MTBH37RV_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:MTBCDC1551_PWY-6383 mono-trans, poly-cis decaprenyl phosphate biosynthesis
BioCyc:ECOL199310_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:MOUSE_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:CORYNE_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:HUMAN_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-6174 mevalonate pathway II (archaea)
BioCyc:META_PWY-7391 isoprene biosynthesis II (engineered)
BioCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:ARA_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:ARA_PWY-7560 methylerythritol phosphate pathway II
BioCyc:ARA_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:MTBCDC1551_PWY-6404 superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis
BioCyc:ECOO157_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL199310_POLYISOPRENSYN-PWY polyisoprenoid biosynthesis (E. coli)
BioCyc:ECOL199310_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:BSUB_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:ECOL316407_POLYISOPRENSYN-PWY polyisoprenoid biosynthesis (E. coli)
BioCyc:HUMAN_PWY-922 mevalonate pathway
BioCyc:META_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:META_POLYISOPRENSYN-PWY polyisoprenoid biosynthesis (E. coli)
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:ECO_UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
BioCyc:MTBCDC1551_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:MOUSE_PWY-922 mevalonate pathway I
BioCyc:BSUB_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:ECOL316407_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:ECOL316407_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:CORYNE_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:CORYNE_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PathBank:SMP0000974 Secondary Metabolites: Methylerythritol Phosphate and Polyisoprenoid Biosynthesis
PathBank:SMP0000991 Secondary Metabolites: Isoprenoid Biosynthesis (Nonmevalonate Pathway)
PlantCyc:ARA_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:ARA_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-922 mevalonate pathway I
PlantCyc:PLANT_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:PLANT_PWY-7560 methylerythritol phosphate pathway II
PlantCyc:ARA_PWY-7560 methylerythritol phosphate pathway II
PlantCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:ARA_PWY-922 mevalonate pathway I
PlantCyc:ARA_NONMEVIPP-PWY methylerythritol phosphate pathway I
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0012042 Epoxysqualene Biosynthesis
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0121210 Terpenoid Backbone Biosynthesis
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0087233 Steroid Biosynthesis
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0121208 MEP/DOXP Pathway
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)
PathBank:SMP0012049 Farnesene Biosynthesis