methylerythritol phosphate pathway I

Feurle J, Espinosa E, Eckstein S, Pont F, Kunzmann V, Fournié JJ, Herderich M, Wilhelm M. Escherichia coli produces phosphoantigens activating human gamma delta T cells. J Biol Chem. 2002 Jan 04;277(1):148–54. doi: 10.1074/jbc.m106443200. PMID: 11675382.; Hecht S, Eisenreich W, Adam P, Amslinger S, Kis K, Bacher A, Arigoni D, Rohdich F. Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein. Proc. Natl. Acad. Sci. U.S.A. 2001 Dec 18;98(26):14837–42. doi: 10.1073/pnas.201399298.; Kim SW, Keasling JD. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol Bioeng. 2001 Feb 20;72(4):408–15. doi: 10.1002/1097-0290(20000220)72:4<408::aid-bit1003>3.0.co;2-h. PMID: 11180061.; Charon L, Hoeffler JF, Pale-Grosdemange C, Lois LM, Campos N, Boronat A, Rohmer M. Deuterium-labelled isotopomers of 2-C-methyl-D-erythritol as tools for the elucidation of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis. Biochem J. 2000 Mar 15;346 Pt 3():737–42. PMID: 10698701; PMCID: PMC1220907.; Lüttgen H, Rohdich F, Herz S, Wungsintaweekul J, Hecht S, Schuhr CA, Fellermeier M, Sagner S, Zenk MH, Bacher A, Eisenreich W. Biosynthesis of terpenoids: YchB protein of Escherichia coli phosphorylates the 2-hydroxy group of 4-diphosphocytidyl-2C-methyl- d -erythritol. Proc. Natl. Acad. Sci. U.S.A. 2000 Feb;97(3):1062–7. doi: 10.1073/pnas.97.3.1062.; Schwender J, Seemann M, Lichtenthaler HK, Rohmer M. Biosynthesis of isoprenoids (carotenoids, sterols, prenyl side-chains of chlorophylls and plastoquinone) via a novel pyruvate/glyceraldehyde 3-phosphate non-mevalonate pathway in the green alga Scenedesmus obliquus. Biochem J. 1996 May 15;316 ( Pt 1)():73–80. PMID: 8645235; PMCID: PMC1217352.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Cobalt ion

Formula: Co (58.933198)

CAS ID: 22541-53-3

Diphosphate

Formula: H4P2O7 (177.9432294)

CAS ID: 2466-09-3

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

iodoacetamide

Formula: C2H4INO (184.9337644)

CAS ID: 144-48-9

pyruvate

Formula: C3H3O3 (87.00821880000001)

CAS ID: 57-60-3

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8



Enzyme

EC Number name full name note
1.1.1.267 1-deoxy-D-xylulose-5-phosphate reductoisomerase 2-C-methyl-D-erythritol-4-phosphate:NADP+ oxidoreductase (isomerizing)
1.17.7.3 (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin) (E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized flavodoxin oxidoreductase
1.17.7.4 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase isopentenyl-diphosphate:ferredoxin oxidoreductase
2.2.1.7 1-deoxy-D-xylulose-5-phosphate synthase pyruvate:D-glyceraldehyde-3-phosphate acetaldehydetransferase (decarboxylating)
2.7.1.148 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ATP:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol 2-phosphotransferase
2.7.7.60 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase CTP:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
4.6.1.12 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming)
5.3.3.2 isopentenyl-diphosphate DELTA-isomerase 3-methylbut-3-en-1-yl-diphosphate DELTA3-DELTA2-isomerase


Proteins

Protein ID name full name
P45568 dxr 1-deoxy-D-xylulose 5-phosphate reductoisomerase
P62615 ispE 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
P62617 ispF 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
P62620 ispG 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)
P62623 ispH 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
P77488 dxs 1-deoxy-D-xylulose-5-phosphate synthase
Q46822 idi Isopentenyl-diphosphate Delta-isomerase
Q46893 ispD 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase