EC: 2.2.1.7

1-deoxy-D-xylulose-5-phosphate synthase (pyruvate:D-glyceraldehyde-3-phosphate acetaldehydetransferase (decarboxylating))

enzyme lambda metabolic reaction experiment
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM TEA, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM Tris, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM TEA, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM phosphate, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM phosphate, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM Tris, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

D_Glyceraldehyde_3_phosphate + Pyruvate -> 1_Deoxy_D_xylulose_5_phosphate + CO2		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37
uniprot:P77488 [Thiamine diphosphate]

Pyruvate + D_Glyceraldehyde_3_phosphate -> CO2 + 1_Deoxy_D_xylulose_5_phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 40 mM HEPES, 1 mg/ml BSA, 2 mM MgCl2, 2.5 mM tris(2-carboxyethyl)phosphine, 100 µM NADPH, 1 µM reductoisomerase IspC
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7392 taxadiene biosynthesis (engineered)
BioCyc:ECO_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:VCHO_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOO157_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL199310_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:AGRO_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:CAULONA1000_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:META_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:META_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:META_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:META_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECOO157_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:SYNEL_PWY-7560 methylerythritol phosphate pathway II
BioCyc:CAULONA1000_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:PDIF272563_PWY-7560 methylerythritol phosphate pathway II
BioCyc:BTHE_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:CORYNE_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:META_PWY-7560 methylerythritol phosphate pathway II
BioCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:ARA_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:ARA_PWY-7560 methylerythritol phosphate pathway II
BioCyc:MTBH37RV_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL199310_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL199310_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:CAULO_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:CAULO_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ANTHRA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:ANTHRA_PWY-7560 methylerythritol phosphate pathway II
BioCyc:AGRO_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:BSUB_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:SYNEL_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:BTHE_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECO_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:ECO_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:MTBCDC1551_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:HPY_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:CAULO_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ANTHRA_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:AGRO_NONMEVIPP-PWY methylerythritol phosphate pathway I
BioCyc:ECOL316407_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis I
BioCyc:ECOL316407_NONMEVIPP-PWY methylerythritol phosphate pathway
BioCyc:ECOL316407_PWY0-845 superpathway of pyridoxal 5'-phosphate biosynthesis and salvage
BioCyc:CAULONA1000_PWY-5121 superpathway of geranylgeranyldiphosphate biosynthesis II (via MEP)
BioCyc:CAULONA1000_PYRIDOXSYN-PWY pyridoxal 5'-phosphate biosynthesis
BioCyc:CORYNE_NONMEVIPP-PWY methylerythritol phosphate pathway I
PathBank:SMP0000974 Secondary Metabolites: Methylerythritol Phosphate and Polyisoprenoid Biosynthesis
PathBank:SMP0000991 Secondary Metabolites: Isoprenoid Biosynthesis (Nonmevalonate Pathway)
PathBank:SMP0002055 Thiazole Biosynthesis I
PlantCyc:PLANT_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:PLANT_PWY-7560 methylerythritol phosphate pathway II
PlantCyc:ARA_PWY-7560 methylerythritol phosphate pathway II
PlantCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:ARA_NONMEVIPP-PWY methylerythritol phosphate pathway I
PathBank:SMP0121328 Secondary Metabolites: Isoprenoid Biosynthesis (Nonmevalonate Pathway)
PathBank:SMP0121325 Secondary Metabolites: Methylerythritol Phosphate and Polyisoprenoid Biosynthesis