EC: 3.1.3.1

alkaline phosphatase (phosphate-monoester phosphohydrolase (alkaline optimum))

enzyme lambda metabolic reaction experiment
uniprot:P00634 [alkaline phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 1 M Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153N]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 1 M Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153A]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 7.5
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153A]

H2O + 2,4_Dinitrophenyl_phosphate -> 2,4_Dinitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 7.5
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153A]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) wildtype]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153N]

4_Nitrophenyl_phosphate + H2O -> Phosphate + p_Nitrophenol		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P00634 [alkaline phosphatase(Enzyme) mutant D153A]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 1 M Tris-HCl, 10 mM MgCl2, 50 µM ZnSO4
PH: 8
Temperature: 24.9
uniprot:P05186 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phosphate + Phenol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM carbonate-bicarbonate, 1 mM MgCl2, 0.06% 4-aminoantipyrin
PH: 10.1
Temperature: 37
uniprot:P09923 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phenol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM carbonate-bicarbonate, 1 mM MgCl2, 0.06% 4-aminoantipyrin
PH: 10.1
Temperature: 37
uniprot:P05187 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phosphate + Phenol		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM carbonate-bicarbonate, 1 mM MgCl2, 0.06% 4-aminoantipyrin
PH: 10.1
Temperature: 37
uniprot:P05186 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phenolic_phosphate -> Phenol + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM carbonate-bicarbonate, 1 mM MgCl2, 0.06% 4-aminoantipyrin
PH: 10.1
Temperature: 37
uniprot:P19111 [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 9.28
Temperature: 30
uniprot:P19111 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.33
Temperature: 30
- [alkaline phosphatase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:P09242 [alkaline phosphatase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
- [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Pyruvate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:P08289 [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:P09242 [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:P08289 [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Phosphate + Pyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:P19111 [alkaline phosphatase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> Pyruvate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6859 all-trans-farnesol biosynthesis
BioCyc:META_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECOL316407_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOL316407_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECOL316407_HISTSYN-PWY histidine biosynthesis
BioCyc:ECOL316407_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation I
BioCyc:ECOL316407_GLUCOSE1PMETAB-PWY glucose and glucose-1-phosphate degradation
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_PWY-7805 (aminomethyl)phosphonate degradation
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:ECO_SALVADEHYPOX-PWY adenosine nucleotides degradation II
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_PWY-6353 purine nucleotides degradation II (aerobic)
BioCyc:ECO_PWY-7805 (aminomethyl)phosphonate degradation
BioCyc:ECOL316407_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:ECOL316407_SERSYN-PWY serine biosynthesis
BioCyc:ECOL316407_SER-GLYSYN-PWY superpathway of serine and glycine biosynthesis I
BioCyc:ECOL316407_SALVADEHYPOX-PWY adenosine nucleotides degradation II
PathBank:SMP0002093 NAD Phosphorylation and Dephosphorylation
PathBank:SMP0002103 Adenosine Nucleotides Degradation
Reactome:R-RNO-1483166 Synthesis of PA
Reactome:R-SCE-1483166 Synthesis of PA
Reactome:R-SCE-597592 Post-translational protein modification
Reactome:R-SCE-163125 Post-translational modification: synthesis of GPI-anchored proteins
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1483206 Glycerophospholipid biosynthesis
Reactome:R-HSA-8935690 Digestion
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-1483166 Synthesis of PA
Reactome:R-RNO-1483206 Glycerophospholipid biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-1483206 Glycerophospholipid biosynthesis
Reactome:R-SCE-8935690 Digestion
Reactome:R-SPO-8935690 Digestion
Reactome:R-SPO-1483257 Phospholipid metabolism
Reactome:R-HSA-1483257 Phospholipid metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8963743 Digestion and absorption
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SPO-1483166 Synthesis of PA
Reactome:R-HSA-8963743 Digestion and absorption
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-1483206 Glycerophospholipid biosynthesis
Reactome:R-RNO-1483257 Phospholipid metabolism
Reactome:R-RNO-8935690 Digestion
Reactome:R-SCE-1483257 Phospholipid metabolism
Reactome:R-SCE-8963743 Digestion and absorption
Reactome:R-SCE-392499 Metabolism of proteins
Reactome:R-SPO-8963743 Digestion and absorption
Reactome:R-SPO-556833 Metabolism of lipids
PathBank:SMP0122247 NAD Phosphorylation and Dephosphorylation
PathBank:SMP0000017 Vitamin B6 Metabolism
PathBank:SMP0000503 Hypophosphatasia