EC: 3.5.4.9

methenyltetrahydrofolate cyclohydrolase (5,10-methenyltetrahydrofolate 5-hydrolase (decyclizing))

enzyme lambda metabolic reaction experiment
- [methenyltetrahydrofolate cyclohydrolase(Enzyme) wildtype]

5,10_Methenyltetrahydrofolate + H2O -> 10_Formyltetrahydrofolate + H+
(( (Vmax * A) ) / (Km + A)) buffer: 0.2 M potassium maleate, 280 mM mercaptoethanol
PH: 6.5
Temperature: 25
uniprot:P51696 [methenyltetrahydrofolate cyclohydrolase(Enzyme) wildtype]

H2O + 5,10_Methenyltetrahydrofolate -> 10_Formyltetrahydrofolate + H+
(( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Triethanolamine hydrochloride, 200 mM 2-Mercaptoethanol
PH: 7.2
Temperature: 22

Pathways

pathway id name
BioCyc:HUMAN_PWY-2161 folate polyglutamylation
BioCyc:META_CODH-PWY reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)
BioCyc:META_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:META_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:META_PWY-2201 folate transformations I
BioCyc:META_PWY-5030 L-histidine degradation III
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:ARA_PWY-3841 folate transformations II
BioCyc:ECOL199310_PWY-2201 folate transformations I
BioCyc:BSUB_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:PDIF272563_P164-PWY purine nucleobases degradation I (anaerobic)
BioCyc:PDIF272563_CODH-PWY reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)
BioCyc:BTHE_PWY-2201 folate transformations I
BioCyc:HUMAN_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:META_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:YEAST_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:ARA_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECOO157_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:ECOO157_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECOL199310_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECOL199310_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:ANTHRA_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ANTHRA_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:AGRO_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MOUSE_PWY-2161 folate polyglutamylation
BioCyc:MOUSE_PWY-2201 folate transformations I
BioCyc:CAULONA1000_PWY-2201 folate transformations
BioCyc:CAULONA1000_PWY-5497 purine degradation II (anaerobic)
BioCyc:CAULONA1000_P164-PWY purine degradation III (anaerobic)
BioCyc:PDIF272563_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:BTHE_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:CORYNE_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:GCF_000013425_PWY-2201 folate transformations I
BioCyc:HUMAN_PWY-5030 histidine degradation
BioCyc:ECO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:YEAST_PWY-2161 folate polyglutamylation
BioCyc:MTBH37RV_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MTBH37RV_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:MTBH37RV_PWY-7909 formaldehyde oxidation VII (THF pathway)
BioCyc:ECOO157_PWY-2201 folate transformations I
BioCyc:ECOO157_PWY-1722 formate reduction to 5,10-methylenetetrahydrofolate
BioCyc:ECOL199310_1CMET2-PWY formylTHF biosynthesis
BioCyc:ANTHRA_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:AGRO_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:MOUSE_PWY-5030 histidine degradation III
BioCyc:MOUSE_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:BSUB_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:SYNEL_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:SYNEL_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:SYNEL_PWY-2161 folate polyglutamylation
BioCyc:SYNEL_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:SYNEL_PWY-2201 folate transformations I
BioCyc:CAULONA1000_PWY-3841 formylTHF biosynthesis II
BioCyc:PDIF272563_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:BTHE_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:CORYNE_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:CORYNE_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:GCF_000013425_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:GCF_000013425_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:HUMAN_PWY-2201 folate transformations I
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:YEAST_PWY-2201 folate transformations
BioCyc:ECOO157_1CMET2-PWY formylTHF biosynthesis
BioCyc:ANTHRA_PWY-2201 folate transformations I
BioCyc:AGRO_PWY-7909 formaldehyde oxidation VII (THF pathway)
BioCyc:AGRO_PWY-2201 folate transformations I
BioCyc:AGRO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MOUSE_PWY-3841 folate transformations II (plants)
BioCyc:BSUB_PWY-2201 folate transformations I
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:SYNEL_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:ECOL316407_1CMET2-PWY formylTHF biosynthesis
BioCyc:CAULONA1000_1CMET2-PWY formylTHF biosynthesis I
BioCyc:CAULONA1000_PWY-1722 formaldehyde oxidation V (tetrahydrofolate pathway)
BioCyc:CAULONA1000_FOLSYN-PWY tetrahydrofolate biosynthesis
BioCyc:PDIF272563_PWY-2201 folate transformations I
BioCyc:PDIF272563_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:PDIF272563_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:BTHE_PWY-1722 formate reduction to 5,10-methylenetetrahydrofolate
BioCyc:GCF_000013425_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:GCF_000013425_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
PathBank:SMP0001750 One Carbon Pool by Folate
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-7909 formaldehyde oxidation VII (THF pathway)
PlantCyc:ARA_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
PlantCyc:ARA_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
PlantCyc:ARA_PWY-3841 folate transformations II
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196757 Metabolism of folate and pterines
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196757 Metabolism of folate and pterines
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196757 Metabolism of folate and pterines
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196757 Metabolism of folate and pterines
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-196757 Metabolism of folate and pterines
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-196757 Metabolism of folate and pterines
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
WikiPathways:WP4259 Disorders of folate metabolism and transport
PathBank:SMP0120742 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0122040 One Carbon Pool by Folate
PathBank:SMP0000432 Methotrexate Action Pathway
PathBank:SMP0000543 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0002428 Glycine Metabolism
PathBank:SMP0087196 Folate Metabolism
PathBank:SMP0120656 Folate Malabsorption, Hereditary
PathBank:SMP0002320 Glycine Metabolism
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis
PathBank:SMP0002443 Serine Metabolism
PathBank:SMP0120875 Folate Malabsorption, Hereditary
PathBank:SMP0120522 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000053 Folate Metabolism
PathBank:SMP0000724 Folate Malabsorption, Hereditary
PathBank:SMP0000801 Folate Metabolism
PathBank:SMP0087295 Folate Metabolism