folate interconversions

Piper MD, Hong SP, Ball GE, Dawes IW. Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J Biol Chem. 2000 Oct 06;275(40):30987–95. doi: 10.1074/jbc.m004248200. PMID: 10871621.; McNeil JB, Bognar AL, Pearlman RE. In Vivo Analysis of Folate Coenzymes and Their Compartmentation in Saccharomyces cerevisiae. 1996 Feb 01;142(2):371–81. doi: 10.1093/genetics/142.2.371.; Appling DR. Compartmentation of folate-mediated one-carbon metabolism in eukaryotes. FASEB J. 1991 Sep;5(12):2645–51. doi: 10.1096/fasebj.5.12.1916088. PMID: 1916088.; Shannon KW, Rabinowitz JC. Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase. Journal of Biological Chemistry. 1988 Jun;263(16):7717–25. doi: 10.1016/s0021-9258(18)68558-8.

Metabolites

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

formate

Formula: CHO2 (44.997654600000004)

CAS ID: 71-47-6

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8

dTMP(2-)

Formula: C10H13N2O8P (320.0409508)

CAS ID: 14057-65-9



Enzyme

EC Number name full name note
1.4.4.2 glycine dehydrogenase (aminomethyl-transferring) glycine:H-protein-lipoyllysine oxidoreductase (decarboxylating, acceptor-amino-methylating)
1.5.1.15 methylenetetrahydrofolate dehydrogenase (NAD+) 5,10-methylenetetrahydrofolate:NAD+ oxidoreductase
1.5.1.20 methylenetetrahydrofolate reductase [NAD(P)H] 5-methyltetrahydrofolate:NAD(P)+ oxidoreductase
1.5.1.3 dihydrofolate reductase 5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase
1.5.1.5 methylenetetrahydrofolate dehydrogenase (NADP+) 5,10-methylenetetrahydrofolate:NADP+ oxidoreductase
1.5.1.53 methylenetetrahydrofolate reductase (NADPH) 5-methyltetrahydrofolate:NADP+ oxidoreductase
1.8.1.4 dihydrolipoyl dehydrogenase protein-N6-(dihydrolipoyl)lysine:NAD+ oxidoreductase
2.1.1.45 thymidylate synthase 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase
2.1.2.1 glycine hydroxymethyltransferase 5,10-methylenetetrahydrofolate:glycine hydroxymethyltransferase
2.1.2.10 aminomethyltransferase [protein]-S8-aminomethyldihydrolipoyllysine:tetrahydrofolate aminomethyltransferase (ammonia-forming)
3.5.4.9 methenyltetrahydrofolate cyclohydrolase 5,10-methenyltetrahydrofolate 5-hydrolase (decyclizing)
6.3.4.3 formate-tetrahydrofolate ligase formate:tetrahydrofolate ligase (ADP-forming)


Proteins

Protein ID name full name
P06785 CDC21 Thymidylate synthase
P07245 ADE3 C-1-tetrahydrofolate synthase, cytoplasmic
P07807 DFR1 Dihydrofolate reductase
P09440 MIS1 C-1-tetrahydrofolate synthase, mitochondrial
P09624 LPD1 Dihydrolipoyl dehydrogenase, mitochondrial
P37291 SHM2 Serine hydroxymethyltransferase, cytosolic
P37292 SHM1 Serine hydroxymethyltransferase, mitochondrial
P46151 MET12 Methylenetetrahydrofolate reductase 1
P48015 GCV1 Aminomethyltransferase, mitochondrial
P49095 GCV2 Glycine dehydrogenase (decarboxylating), mitochondrial
P53128 MET13 Methylenetetrahydrofolate reductase 2
Q02046 MTD1 Methylenetetrahydrofolate dehydrogenase [NAD(+)]