EC: 2.1.2.10

aminomethyltransferase ([protein]-S8-aminomethyldihydrolipoyllysine:tetrahydrofolate aminomethyltransferase (ammonia-forming))

enzyme lambda metabolic reaction experiment
- [aminomethyltransferase(Enzyme) wildtype]

Glycine + 5,6,7,8_Tetrahydrofolate + NAD+ -> CO2 + 5,10_Methylenetetrahydrofolate + NADH + NH3 + H+		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 5 mM MOPS, 5 mM Tris, 1 mM ß-mercaptoethanol, 1 mM MgCl2, 1 mM EGTA, 20 µM Pyridoxalphosphate
PH: 7.2
Temperature: 25

Pathways

pathway id name
BioCyc:META_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:META_PWY-2201 folate transformations I
BioCyc:META_GLYCLEAV-PWY glycine cleavage
BioCyc:YEAST_GLYCLEAV-PWY glycine cleavage
BioCyc:YEAST_GLYCINE-SYN2-PWY glycine biosynthesis II
BioCyc:ECOL199310_GLYCLEAV-PWY glycine cleavage
BioCyc:ECOL199310_PWY-2201 folate transformations I
BioCyc:BSUB_GLYCLEAV-PWY glycine cleavage
BioCyc:SYNEL_PWY-181 photorespiration
BioCyc:SYNEL_GLYCLEAV-PWY glycine cleavage
BioCyc:META_GLYCINE-SYN2-PWY glycine biosynthesis II
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:ECOL199310_GLYCINE-SYN2-PWY glycine biosynthesis II
BioCyc:MOUSE_GLYCLEAV-PWY glycine cleavage complex
BioCyc:BTHE_GLYCINE-SYN2-PWY glycine biosynthesis II
BioCyc:BTHE_GLYCLEAV-PWY glycine cleavage
BioCyc:GCF_000013425_PWY-2201 folate transformations I
BioCyc:ECO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECOO157_PWY-2201 folate transformations I
BioCyc:ECOL199310_1CMET2-PWY formylTHF biosynthesis
BioCyc:ANTHRA_GLYCLEAV-PWY glycine cleavage
BioCyc:MOUSE_PWY3DJ-35778 Serine degradation II
BioCyc:SYNEL_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:SYNEL_PWY-2201 folate transformations I
BioCyc:GCF_000013425_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:GCF_000013425_GLYCLEAV-PWY glycine cleavage
BioCyc:HUMAN_GLYCLEAV-PWY glycine cleavage
BioCyc:ECO_GLYCLEAV-PWY glycine cleavage
BioCyc:YEAST_PWY-2201 folate transformations
BioCyc:MTBH37RV_GLYCLEAV-PWY glycine cleavage
BioCyc:ECOO157_1CMET2-PWY formylTHF biosynthesis
BioCyc:ECOO157_GLYCLEAV-PWY glycine cleavage
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0001750 One Carbon Pool by Folate
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-BTA-6783984 Glycine degradation
Reactome:R-CFA-1430728 Metabolism
Reactome:R-DDI-6783984 Glycine degradation
Reactome:R-HSA-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-BTA-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-CFA-71291 Amino acid and derivative metabolism
Reactome:R-CFA-6783984 Glycine degradation
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-DDI-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-6783984 Glycine degradation
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-6783984 Glycine degradation
Reactome:R-SCE-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-SPO-6783984 Glycine degradation
Reactome:R-CFA-389661 Glyoxylate metabolism and glycine degradation
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-6783984 Glycine degradation
PathBank:SMP0000009 Ammonia Recycling
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0002369 One Carbon Pool by Folate I
PathBank:SMP0087177 Ammonia Recycling
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0120653 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0120475 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0002428 Glycine Metabolism
PathBank:SMP0087245 Glycine and Serine Metabolism
PathBank:SMP0120521 Sarcosinemia
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0002320 Glycine Metabolism
PathBank:SMP0002443 Serine Metabolism
PathBank:SMP0120474 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0120534 Non-Ketotic Hyperglycinemia
PathBank:SMP0120550 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0063626 Glycine and Serine Metabolism