EC: 2.1.1.45

thymidylate synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82S]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82H]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82Y]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82F]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82G]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> dUMP + Br_ + Hydroxyethyl_disulfide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82R]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> Hydroxyethyl_disulfide + dUMP + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82D]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82V]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Br_ + Hydroxyethyl_disulfide + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82M]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82S]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82N]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82A]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82L]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82H]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82Y]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82F]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (Vmax * A) ) * B) ) / (( (( (( (( (( (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B)) ) + (( (( (Kia * Kb) ) * Q) ) / Kiq)) ) + (( (( (( (Kia * Kb) ) * Kq) ) * P) ) / (Kp * Kiq))) ) + (( (( (( (Kb * Kq) ) * A) ) * P) ) / (Kp * Kiq))) ) + (( (( (Ka * B) ) * Q) ) / Kiq)) ) + (( (( (A * B) ) * P) ) / Kip))) buffer: 0.1 M Pipes, 5 mM bicarbonate, 6.75 mM formaldehyde, 25 mM 2-mercaptoethanol
PH: 6.8
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82M]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82A]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P0A884 [thymidylate synthase(Enzyme) mutant I264Am]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (k1 / (( (( (K1 / S) ) + 1) ) + (S / K2))) ) + (k2 / (( (( (( (K1 * K2) ) / (S ^ 2)) ) + 1) ) + (K2 / S)))) ) * E) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316R]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (k1 / (( (( (K1 / S) ) + 1) ) + (S / K2))) ) + (k2 / (( (( (( (K1 * K2) ) / (S ^ 2)) ) + 1) ) + (K2 / S)))) ) * E) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316G]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (k1 / (( (( (K1 / S) ) + 1) ) + (S / K2))) ) + (k2 / (( (( (( (K1 * K2) ) / (S ^ 2)) ) + 1) ) + (K2 / S)))) ) * E) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316Am]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (k1 / (( (( (K1 / S) ) + 1) ) + (S / K2))) ) + (k2 / (( (( (( (K1 * K2) ) / (S ^ 2)) ) + 1) ) + (K2 / S)))) ) * E) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82S]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> Hydroxyethyl_disulfide + dUMP + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82H]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Br_ + Hydroxyethyl_disulfide + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82F]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Hydroxyethyl_disulfide + Br_ + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82Y]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Hydroxyethyl_disulfide + Br_ + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82M]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82N]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Br_ + Hydroxyethyl_disulfide + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82L]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Br_ + dUMP + Hydroxyethyl_disulfide		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) mutant W82A]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> Hydroxyethyl_disulfide + dUMP + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 22
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant N229D]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant N229D]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant N229D]

5,10_Methylenetetrahydrofolate + dCMP -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant N229D]

5,10_Methylenetetrahydrofolate + dCMP -> Dihydrofolate + 5_Methyldeoxycytidine_5'_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316R]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316Am]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [thymidylate synthase(Enzyme) mutant V316G]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P0A884 [thymidylate synthase(Enzyme) mutant I264Am]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 25 mM MgCl2, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P45352 [Methotrexate]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (Vmax * B) ) / (( (Km * (1 + (I / Ki))) ) + (B * (1 + (I / Ki))))) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [dTMP]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + (A * (1 + (I / Ki))))) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [dTMP]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (Vmax * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [5-Fluoro-dUMP]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (Vmax * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [Methotrexate]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [Cibacron blue 3GA]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P45352 [Cibacron blue 3GA]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (Vmax * B) ) / (( (Km * (1 + (I / Ki))) ) + B)) buffer: 50 mM Tris-HCl, 2 mM Dithiothreitol, 0.15 M NaCl
PH: 7.3
Temperature: 37
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel5 (delta90-139, F87D)]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel5 (delta90-139, F87D)]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel4]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel6]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel6]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM Formaldehyde, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 20
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel1 (delta90-139)]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel2 (delta90-139, K89N)]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> Br_ + Hydroxyethyl_disulfide + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel4]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel6]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> Hydroxyethyl_disulfide + Br_ + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel5]

5_Bromo_dUMP + 2 2_Mercaptoethanol -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P00469 [thymidylate synthase(Enzyme) mutant TSDel3]

2 2_Mercaptoethanol + 5_Bromo_dUMP -> dUMP + Hydroxyethyl_disulfide + Br_		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM N-Methylmorpholine hydrochloride, 25 mM MgCl2, 1 mM EDTA, 75 mM beta-Mercaptoethanol
PH: 7.4
Temperature: 30
uniprot:P0A886 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) wildtype]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant G257K]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant E86K]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant G23K-E86K]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant E86K]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant G257K]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P0A886 [thymidylate synthase(Enzyme) mutant G23K-E86K]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM TES, 25 mM MgCl2, 6.5 mM HCHO, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 25
uniprot:P04818 [E-5-(2-Bromovinyl)-2'-deoxyuridine monophosphate]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (( (Km_A * (1 + (I / Ki))) ) + A)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA, 100 mM beta-mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:P04818 [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km_A + A)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA, 100 mM beta-mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:P04818 [thymidylate synthase(Enzyme) wildtype]

E_5_(2_Bromovinyl)_2'_deoxyuridine_monophosphate + 2_Mercaptoethanol -> 1_(3_Hydroxy_4_hydroxymethyl_cyclopentyl)_5_[1,4]oxathian_2_yl_1H_pyrimidine_2,4_dione		 (( (( (E * kcat) ) * A) ) / (Km_A + A)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P04818 [E-5-(2-Bromovinyl)-2'-deoxyuridine monophosphate]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (( (Km_A * (1 + (I / Ki))) ) + A)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA, 100 mM beta-mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:P04818 [thymidylate synthase(Enzyme) wildtype]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * B) ) / (Km_B + B)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA, 100 mM beta-mercaptoethanol
PH: 7.5
Temperature: 30
uniprot:P04818 [5,10-Methylenetetrahydrofolate]

E_5_(2_Bromovinyl)_2'_deoxyuridine_monophosphate + 2_Mercaptoethanol -> 1_(3_Hydroxy_4_hydroxymethyl_cyclopentyl)_5_[1,4]oxathian_2_yl_1H_pyrimidine_2,4_dione		 (( (( (E * kcat) ) * A) ) / (( (A * (1 + (I / Ki))) ) + (Km_A * (1 + (I / Ki))))) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P04818 [dUMP]

2_Mercaptoethanol + E_5_(2_Bromovinyl)_2'_deoxyuridine_monophosphate -> 1_(3_Hydroxy_4_hydroxymethyl_cyclopentyl)_5_[1,4]oxathian_2_yl_1H_pyrimidine_2,4_dione		 (( (( (E * kcat) ) * A) ) / (( (Km_A * (1 + (I / Ki))) ) + A)) buffer: 40 mM Tris-HCl, 25 mM MgCl2, 1 mM EDTA
PH: 7.5
Temperature: 30
- [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ks))))) buffer: 100 mM Tris, 50 mM beta-mercaptoethanol, 1 mM EDTA, 5 mM CH2O
PH: 7.5
Temperature: 20
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dCMP -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dCMP + 5,10_Methylenetetrahydrofolate -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dCMP -> Dihydrofolate + 5_Methyldeoxycytidine_5'_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dCMP + 5,10_Methylenetetrahydrofolate -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dCMP + 5,10_Methylenetetrahydrofolate -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dCMP -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dCMP -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
uniprot:P00469 [Mg2+]

5,10_Methylenetetrahydrofolate + dCMP -> 5_Methyldeoxycytidine_5'_phosphate + Dihydrofolate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM TES, 6.5 mM formaldehyde, 1 mM EDTA, 75 mM beta-mercaptoethanol
PH: 7.4
Temperature: 36
- [thymidylate synthase(Enzyme) wildtype MTX-resistant]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 5 mM H2CO, 1 mg/ml BSA
PH: 7.8
Temperature: 25
- [thymidylate synthase(Enzyme) wildtype MTX-resistant]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 5 mM H2CO, 1 mg/ml BSA
PH: 7.8
Temperature: 25
- [thymidylate synthase(Enzyme) wildtype MTX-resistant]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 5 mM H2CO, 1 mg/ml BSA
PH: 7.8
Temperature: 25
- [thymidylate synthase(Enzyme) mutant S167T]

5,10_Methylenetetrahydrofolate + dUMP -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167C]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167G]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) wildtype]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167A]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167T]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167G]

dUMP + 5,10_Methylenetetrahydrofolate -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167C]

dUMP + 5,10_Methylenetetrahydrofolate -> dTMP + Dihydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) mutant S167A]

5,10_Methylenetetrahydrofolate + dUMP -> Dihydrofolate + dTMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 27
- [thymidylate synthase(Enzyme) wildtype]

1,4_Dithiothreitol + 5_Bromo_dUMP -> Oxidized_dithiothreitol + Hydrobromic_acid + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 25
- [thymidylate synthase(Enzyme) mutant S167G]

5_Bromo_dUMP + 1,4_Dithiothreitol -> Hydrobromic_acid + Oxidized_dithiothreitol + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 25
- [thymidylate synthase(Enzyme) mutant S167A]

5_Bromo_dUMP + 1,4_Dithiothreitol -> Hydrobromic_acid + Oxidized_dithiothreitol + dUMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM Acetic acid
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:META_PWY-3841 folate transformations II
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECO_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-3841 folate transformations II
BioCyc:VCHO_PWY-3841 folate transformations II
BioCyc:VCHO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:MTBH37RV_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:ECOL199310_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:CAULO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ANTHRA_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:ANTHRA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:AGRO_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:BSUB_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOL316407_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:BTHE_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:BTHE_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:GCF_000013425_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:YEAST_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:VCHO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:CAULO_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ANTHRA_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ANTHRA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:MOUSE_P1-PWY purine and pyrimidine metabolism
BioCyc:BSUB_P1-PWY purine and pyrimidine metabolism
BioCyc:BSUB_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:BTHE_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:BTHE_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:CORYNE_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:CORYNE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY66-385 dTMP de novo biosynthesis (mitochondrial)
BioCyc:META_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:META_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:VCHO_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBH37RV_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECOO157_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:ECOL199310_1CMET2-PWY formylTHF biosynthesis
BioCyc:ECOL199310_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:CAULO_1CMET2-PWY formylTHF biosynthesis I
BioCyc:CAULO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-3841 folate transformations II
BioCyc:AGRO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:BTHE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:GCF_000013425_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:GCF_000013425_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:GCF_000013425_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:META_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY66-385 dTMP de novo biosynthesis (mitochondrial)
BioCyc:YEAST_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:VCHO_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:MTBH37RV_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:MTBH37RV_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOO157_1CMET2-PWY formylTHF biosynthesis
BioCyc:ECOL199310_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ANTHRA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ANTHRA_PWY0-166 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)
BioCyc:AGRO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:AGRO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:AGRO_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MOUSE_PWY-3841 folate transformations II (plants)
BioCyc:ECOL316407_1CMET2-PWY formylTHF biosynthesis
BioCyc:CORYNE_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:GCF_000013425_PWY-7199 pyrimidine deoxyribonucleosides salvage
PathBank:SMP0000959 Pyrimidine Metabolism
PathBank:SMP0001750 One Carbon Pool by Folate
PlantCyc:SOY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:SOY_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:SOY_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:PLANT_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:SOY_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
PlantCyc:SOY_PWY-3841 folate transformations II
PlantCyc:SOY_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
PlantCyc:SOY_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-3841 folate transformations II
PlantCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-3841 folate transformations II
PlantCyc:PLANT_PWY-6612 superpathway of tetrahydrofolate biosynthesis
Reactome:R-HSA-69206 G1/S Transition
Reactome:R-HSA-69205 G1/S-Specific Transcription
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-SPO-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-SCE-1430728 Metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-DME-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SCE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-453279 Mitotic G1 phase and G1/S transition
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-499943 Interconversion of nucleotide di- and triphosphates
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
WikiPathways:WP4688 Serine metabolism
WikiPathways:WP4259 Disorders of folate metabolism and transport
PathBank:SMP0121324 Pyrimidine Metabolism
PathBank:SMP0122040 One Carbon Pool by Folate
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000469 Capecitabine Action Pathway
PathBank:SMP0002369 One Carbon Pool by Folate I
PathBank:SMP0012014 Folate Biosynthesis
PathBank:SMP0012077 Pyrimidine Deoxyribonucleosides Salvage
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0000608 Fluorouracil Metabolism Pathway
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000470 Fluorouracil Action Pathway
PathBank:SMP0002370 Pyrimidine Metabolism
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0000603 Gemcitabine Metabolism Pathway
PathBank:SMP0000607 Capecitabine Metabolism Pathway