EC: 3.1.3.11

fructose-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P0A993 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+
PH: 7.5
Temperature: 22
uniprot:P0A993 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+
PH: 7.5
Temperature: 22
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.2
Temperature: 36
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 36
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 36
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.2
Temperature: 36
uniprot:P0A993 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (V0 + (( (( (Vmax - V0) ) * A) ) / (A_half + A))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+.
PH: 7.5
Temperature: 22
uniprot:P0A993 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+
PH: 7.5
Temperature: 22
uniprot:P0A993 [Citrate]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (V0 + (( (( (Vmax - V0) ) * A) ) / (A_half + A))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+.
PH: 7.5
Temperature: 22
uniprot:P0A993 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (V0 + (( (( (Vmax - V0) ) * A) ) / (A_half + A))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+.
PH: 7.5
Temperature: 22
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.2
Temperature: 36
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 36
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.2
Temperature: 36
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP, 0.6 U/ml Glucose 6-phosphate dehydrogenase, 1.2 U/ml Phosphoglucose isomerase
PH: 8.8
Temperature: 36
uniprot:P00636 [AMP]

H2O + Fructose_1,6_bisphosphate -> Fructose_6_phosphate + Phosphate		 (Vmax / (( (( (1 + (Ka / (A ^ 2))) ) + (( (Ka * (I ^ 2)) ) / (Ki * (A ^ 2)))) ) + (( (( (I ^ 2) ) / Kii) ) * (1 + (B / Kiis))))) buffer: 50 mM Hepes
PH: 7.5
Temperature: 22
uniprot:P00636 [Mg2+]

H2O + Fructose_1,6_bisphosphate -> Phosphate + Fructose_6_phosphate		 (Vmax / (( (1 + (Kb / B)) ) + (( (( (I ^ 2) ) / Kii) ) * (1 + (B / Kiis))))) buffer: 50 mM Hepes
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (Vmax / (( (1 + (Km / (A ^ 2))) ) + (( (Km / Ki) ) * (( (I / A) ) ^ 2)))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (Vmax / (( (1 + (Km / (A ^ 2))) ) + (( (Km / Ki) ) * (( (I / A) ) ^ 2)))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Fructose 2,6-bisphosphate]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (Vmax / (( (1 + (Km / A)) ) + (( (Km / Ki) ) * (I / A)))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (Vmax / (( (1 + (Km / A)) ) + (( (Km / Ki) ) * (I / A)))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Hepes, 10 µM EDTA, 150 mM KCl
PH: 7.5
Temperature: 22
- [Mn2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole-HCl, 0.4 mM NAD+, 1 U/ml glucose-6-phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7.5
Temperature: 22
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole-HCl, 0.4 mM NAD+, 1 U/ml glucose-6-phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7.5
Temperature: 22
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole-HCl, 0.4 mM NAD+, 1 U/ml glucose-6-phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7.5
Temperature: 22
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
- [1,4-Dithiothreitol]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [1,4-Dithiothreitol]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Ca2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:P22418 [Ca2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
uniprot:Q8RW99 [fructose-bisphosphatase(Enzyme) wildtype His-tagged]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.2 mM EDTA, 5 mM MgCl2, 0.3 mM NADP, 1.2 U phosphoglucose isomerase, 0.9 U glucose-6-phosphate dehydrogenase
PH: 6.6
Temperature: 25
uniprot:Q8RW99 [fructose-bisphosphatase(Enzyme) wildtype]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M MES, 0.2 mM EDTA, 5 mM MgCl2, 0.3 mM NADP, 1.2 U phosphoglucose isomerase, 0.9 U glucose-6-phosphate dehydrogenase
PH: 6.6
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
- [1,4-Dithiothreitol]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
uniprot:P22418 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (M ^ n)) ) / (( (S_half ^ n) ) + (M ^ n))) buffer: 100 mM Tris-Cl, 0.1 mM EGTA-Na, 0.3 mM NADP, 1.75 U/ml phosphoglucose isomerase, 0.7 U/ml glucose-6-phosphate dehydrogenase
PH: 7.9
Temperature: 25
uniprot:P22418 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 1.5 U/ml phosphoglucose isomerase, 0.5 U/ml glucose 6-phosphate dehydrogenase
PH: 8
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
- [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.3 mM NADP+, 3 units/ml glucose-6-phosphate isomerase, 1 units/ml glucose 6-phosphate dehydrogenase, 0.03 % Triton X-100
PH: 8
Temperature: 25
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 7.5
Temperature: 25
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 8.5
Temperature: 22
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (1 + (( (I ^ n) ) / Kii)) ) + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ n) ) / Ki)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 8.5
Temperature: 22
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ n) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (( (Kia / (A ^ 2)) ) * Kb) ) / B) ) * (( (1 + (I / Ki)) ) + (( (I ^ n) ) / Kii)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 8.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / (kcat * E)) ) * (( (1 + (( (I ^ n) ) / Kii)) ) + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ n) ) / Ki)))))) buffer: 50 mM Tris, 0.1 M KCl
PH: 8.5
Temperature: 22
- [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / Vmax) ) * (( (( (1 + (( (Ka / A) ) * (1 + (( (I ^ 2) ) / Kiiz)))) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A * B)) ) * (1 + (( (I ^ 2) ) / Kiz)))))) buffer: 50 mM diethanolamine, 0.1 M KCl
PH: 9.5
Temperature: 25
- [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (1 / (( (1 / Vmax) ) * (( (( (1 + (( (Ka / A) ) * (1 + (I / Kiiy)))) ) + (Kb / B)) ) + (( (( (Kia * kb) ) / (A * B)) ) * (1 + (I / Kiy)))))) buffer: 50 mM Diethanolamine, 0.1 M KCl
PH: 9.5
Temperature: 25
- [Cd2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (1 / (( (1 / Vmax) ) * (( (( (1 + (Ka / A)) ) + (( (Kb / B) ) * (1 + (I / Kiix)))) ) + (( (( (Kia * Kb) ) / (A * B)) ) * (1 + (I / Kix)))))) buffer: 50 mM Diethanolamine, 0.1 M KCl
PH: 9.5
Temperature: 25
- [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 50 mM Diethanolamine, 0.1 M KCl
PH: 9.5
Temperature: 25
- [fructose-bisphosphatase(Enzyme) wildtype]

D_Fructose_6_phosphate + Phosphate -> H2O + D_Fructose_1,6_bisphosphate		 (V / (( (( (1 + (Kq / Q)) ) + (Kp / P)) ) + (( (Kiq * Kp) ) / (P * Q)))) buffer: 25 mM Tris, 25 mM Histidine, 8 mM Mg2+, 125 µM NADH, 4.6 units aldolase, 29 units triosephosphate isomerase, 2.6 units glycerolphosphate dehydrogenase
PH: 6.5
Temperature: 28
- [fructose-bisphosphatase(Enzyme) wildtype]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (V / (1 + (( (Ka / A) ) * (1 + (Q / Kiq))))) buffer: 25 mM Tris, 25 mM Histidine, 5 mM MgSO4, 190 µM NAD, 6.3 units phosphoglucoisomerase, 4.3 units glucose-6-phosphate dehydrogenase, 190 µM NAD, 6.3 units phosphoglucoisomerase, 4.3 units glucose-6-phosphate dehydrogenase
PH: 6.5
Temperature: 28
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki_S))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki_S))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki_S))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki_S))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ki_S))))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ki_S))))) buffer: 100 mM Tris/HCl, 2 mM (NH4)2SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (A ^ h)) ) / (( (S_h ^ h) ) + (A ^ h))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))) ) + (S * (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))) ) + (S * (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 100 mM Tris/HCl, 2 mM NH4SO4, 2.5 mM 2-mercaptoethanol, 0.05 mM EDTA, 0.2 mM NADP+
PH: 7.5
Temperature: 30
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (( (E * kcat) ) * (A ^ 2)) ) * B) ) / (( (( (( (( (A ^ 2) ) * B) ) + (Ka * B)) ) + (Kb * (A ^ 2))) ) + (Kia * Kb))) buffer: 50 mM Hepes, 150 mM KCl, 0.1 mM EDTA
PH: 7.5
Temperature: 22
uniprot:P00636 [D-Fructose 2,6-bisphosphate]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (( (E * kcat) ) * (A ^ 2)) ) * B) ) / (( (( (( (Ka * B) ) + (( (Kia * Kb) ) * (1 + (I / Kiy)))) ) + (( (A ^ 2) ) * B)) ) + (( (Kb * (1 + (I / Kiiy))) ) * (A ^ 2)))) buffer: 50 mM Hepes, 150 mM KCl, 0.1 µM or 0.1 mM EDTA
PH: 7.5
Temperature: 22
uniprot:P00636 [Cd2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (E * kcat) ) * (( (A ^ 2) ) * B)) ) / (( (( (( (Kb * (A ^ 2)) ) + (( (Kia * Kb) ) * (1 + (I / Kix)))) ) + (( (A ^ 2) ) * B)) ) + (( (Ka * (1 + (I / Kiix))) ) * B))) buffer: 50 mM Hepes, 150 mM KCl, 0.1 µM EDTA
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (( (( (1 + (Ka / (A ^ 2))) ) + (Kb / B)) ) + (( (Kab / (A ^ 2)) ) * B))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (( (( (1 + (Ka / (A ^ 2))) ) + (Kb / B)) ) + (( (Kab / (A ^ 2)) ) * B))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (( (( (1 + (Ka / (A ^ 2))) ) + (Kb / B)) ) + (( (Kab / (A ^ 2)) ) * B))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (1 + (( (Ka / (A ^ 2)) ) * (1 + (I / Ki))))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (1 + (( (Ka / (A ^ 2)) ) * (1 + (( (I ^ 2) ) / Ki))))) buffer: Hepes
PH: 7.5
Temperature: 24
uniprot:P0A9C9 [Mn2+]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tricine, 50 mM KCl, 0.25 mM NADP, 0.2 U/ml Phosphoglucose isomerase, 0.4 U/ml Glucose 6-phosphate dehydrogenase
PH: 7.7
Temperature: 22
uniprot:Q6M6E7 [Mn2+]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tricine, 100 mM KCl, 0.25 mM NADP+, 0.4 U/ml Yeast Glucose 6-phosphate dehydrogenase, 0.7 U/ml Yeast Phosphoglucoisomerase
PH: 7.7
Temperature: 30
uniprot:Q6M6E7 [Mn2+]

H2O + Fructose_1,6_bisphosphate -> Fructose_6_phosphate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tricine, 100 mM KCl, 0.25 mM NADP+, 0.4 U/ml Yeast Glucose 6-phosphate dehydrogenase, 0.7 U/ml Yeast Phosphoglucoisomerase
PH: 7.7
Temperature: 30
uniprot:O00757 [fructose-bisphosphatase(Enzyme) wildtype]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [fructose-bisphosphatase(Enzyme) mutant T177M/Q179C]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [fructose-bisphosphatase(Enzyme) mutant K20E]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [fructose-bisphosphatase(Enzyme) mutant K20E/T177M/Q179C]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [fructose-bisphosphatase(Enzyme) wildtype]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [fructose-bisphosphatase(Enzyme) mutant T177M/Q179C]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [fructose-bisphosphatase(Enzyme) mutant K20E/T177M/Q179C]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [fructose-bisphosphatase(Enzyme) mutant K20E]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:O00757 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
uniprot:P09467 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * (I ^ n)) ) / (( (I_half ^ n) ) + (I ^ n))) buffer: 50 mM BTP, 2 mM MgCl2, 150 mM KCl, 1 mM EDTA, 0.2 mM NADP+, 10 U/ml Glucose 6-phosphate dehydrogenase, 10 U/ml Glucose 6-phosphate isomerase
PH: 7.5
Temperature: 37
- [fructose-bisphosphatase(Enzyme) wildtype]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 150 mM KCl
PH: 7.6
Temperature: 37
uniprot:P00636 [AMP]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (Vmax * (S_half ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+, 150 mM KCl; coupling assay: phosphoglucose isomerase and glucose-6 phosphate dehydrogenase, measurement of formation of NADPH
PH: 7.5
Temperature: 22
uniprot:P0A993 [AMP]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (Vmax * (S_half ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+; coupling assay: phosphoglucose isomerase and glucose-6 phosphate dehydrogenase, measurement of formation of NADPH
PH: 7.5
Temperature: 22
uniprot:P0A993 [AMP]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (Vmax * (S_half ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+; coupling assay: phosphoglucose isomerase and glucose-6 phosphate dehydrogenase, measurement of formation of NADPH.
PH: 7.5
Temperature: 22
uniprot:P00636 [AMP]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (Vmax * (S_half ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 50 mM Hepes, 100 µM EDTA, 150 µM NADP+, 150 mM KCl; coupling assay: phosphoglucose isomerase and glucose-6 phosphate dehydrogenase, measurement of formation of NADPH
PH: 7.5
Temperature: 22
- [fructose-bisphosphatase(Enzyme) wildtype]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM triethanolamine/diethanolamine, 5 mM MgCl2, 0.2 mM NADP, 2 µg/ml phosphoglucose isomerase, 2 µg/ml glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 7.5
- [fructose-bisphosphatase(Enzyme) wildtype]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 40 mM triethanolamine/diethanolamine, 5 mM MgCl2, 0.2 mM NADP, 2 µg/ml phosphoglucose isomerase, 2 µg/ml glucose-6-phosphate dehydrogenase
PH: 7.4
Temperature: 25
uniprot:P14766 [fructose-bisphosphatase(Enzyme) wildtype]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole-HCl, 1 mM MgSO4, 1 mM NADP, 0.2 units/ml Glucose 6-phosphate dehydrogenase, 0.4 units/ml Phosphoglucose isomerase
PH: 7.5
Temperature: 36
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [Mg2+]

H2O + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + Phosphate		 (( (E * kcat) ) / (1 + (( (( (Ka / (A ^ h)) ) + (Kb / B)) ) + (( (( (Kia * Kb) ) / (A ^ h)) ) * B)))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00636 [AMP]

D_Fructose_1,6_bisphosphate + H2O -> Phosphate + D_Fructose_6_phosphate		 (( (E * kcat) ) / (( (( (1 + (( (Ka / (A ^ 2)) ) * (( (( (( (I + (I / Ki)) ) + (( (I ^ h) ) / Kii)) ) + (I / Kiii)) ) + (( (I ^ h) ) / Kiv)))) ) + (Kb / B)) ) + (( (( (Kia / (A ^ 2)) ) * B) ) * (( (1 + (I / Ki)) ) + (( (I ^ h) ) / Kii))))) buffer: 50 mM Hepes, 0.1 M KCl
PH: 7.5
Temperature: 24
uniprot:P00637 [fructose-bisphosphatase(Enzyme) wildtype]

D_Fructose_1,6_bisphosphate + H2O -> beta_D_Fructose_6_phosphate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM EDTA, glucose 6-phosphate dehydrogenase, NADP+
PH: 9.4
Temperature: 36
uniprot:P09467 [AMP]

H2O + Fructose_1,6_bisphosphate -> Phosphate + Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris/HCl, 0.2 mM EDTA, 0.2 mM NADP, 7.0 U/ml phosphoglucose isomerase, 3.5 U/ml glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris/HCl, 0.2 mM EDTA, 0.2 mM NADP, 7.0 U/ml phosphoglucose isomerase, 3.5 U/ml glucose-6-phosphate dehydrogenase
PH: 7.5
Temperature: 37
uniprot:P09467 [Mg2+]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris/HCl, 0.2 mM EDTA, 0.2 mM NADP, 7.0 U/ml phosphoglucose isomerase, 3.5 U/ml glucose-6-phosphate dehydrogenase
PH: 9.5
Temperature: 37
uniprot:A0A5B9GD00 [AMP]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (Ki ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 0.5 mM NADP+, 1 U/ml glucose-6-phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7.5
Temperature: 22
uniprot:A0A5B9GD00 [Mg2+]

H2O + Fructose_1,6_bisphosphate -> Phosphate + Fructose_6_phosphate		 (( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 50 mM Tris-HCl, 0.5 mM NADP+, 1 U/ml glucose-6-phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7.5
Temperature: 22
uniprot:Q57573 [fructose-bisphosphatase(Enzyme) wildtype]

Fructose_1,6_bisphosphate + H2O -> Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 20 mM MgCl2
PH: 8.5
Temperature: 85
uniprot:O30298 [fructose-bisphosphatase(Enzyme) wildtype]

H2O + Fructose_1,6_bisphosphate -> Fructose_6_phosphate + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 20 mM MgCl2
PH: 8.5
Temperature: 85
uniprot:P22255 [fructose-bisphosphatase(Enzyme) wildtype His6-tag]

Fructose_1,6_bisphosphate + H2O -> Phosphate + Fructose_6_phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q59943 [Mg2+]

H2O + beta_D_Fructose_1,6_bisphosphate -> beta_D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (A ^ h)) ) / (( (S_half ^ h) ) + (A ^ h))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.4 mM NADP+, 0.5 U/ml glucose-6-phosphate dehydrogenase, 1.5 U/ml phosphoglucose isomerase
PH: 8
Temperature: 36
uniprot:Q31QY2 [Mg2+]

beta_D_Fructose_1,6_bisphosphate + H2O -> beta_D_Fructose_6_phosphate + Phosphate		 (( (Vmax * (A ^ h)) ) / (( (S_half ^ h) ) + (A ^ h))) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.4 mM NADP+, 0.5 U/ml glucose-6-phosphate dehydrogenase, 1.5 U/ml phosphoglucose isomerase
PH: 8
Temperature: 36
uniprot:Q31QY2 [Mg2+]

H2O + beta_D_Fructose_1,6_bisphosphate -> Phosphate + beta_D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.4 mM NADP+, 0.5 U/ml glucose-6-phosphate dehydrogenase, 1.5 U/ml phosphoglucose isomerase
PH: 8
Temperature: 36
uniprot:Q59943 [Mg2+]

H2O + beta_D_Fructose_1,6_bisphosphate -> Phosphate + beta_D_Fructose_6_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl, 0.5 mM EDTA, 0.4 mM NADP+, 0.5 U/ml glucose-6-phosphate dehydrogenase, 1.5 U/ml phosphoglucose isomerase
PH: 8
Temperature: 36

Pathways

pathway id name
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:META_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:META_PWY-2301 myo-inositol biosynthesis
BioCyc:META_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:ECO_GLUCONEO-PWY gluconeogenesis I
BioCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:VCHO_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:VCHO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:MTBH37RV_NAD-BIOSYNTHESIS-II NAD salvage pathway III (to nicotinamide riboside)
BioCyc:MTBH37RV_NADPHOS-DEPHOS-PWY NAD phosphorylation and dephosphorylation
BioCyc:HPY_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOO157_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOO157_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:CAULO_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:CAULO_GLUCONEO-PWY gluconeogenesis I
BioCyc:CAULO_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:MOUSE_GLYCOLYSIS glycolysis I
BioCyc:MOUSE_GLUCONEO-PWY gluconeogenesis I
BioCyc:SYNEL_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOL316407_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOL316407_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:BTHE_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:BTHE_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:GCF_000013425_GLUCONEO-PWY gluconeogenesis I
BioCyc:HUMAN_PWY66-399 gluconeogenesis
BioCyc:META_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:META_PWY-7345 superpathway of anaerobic sucrose degradation
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ARA_GLUCONEO-PWY gluconeogenesis I
BioCyc:MTBH37RV_PWY-6348 phosphate acquisition
BioCyc:MTBH37RV_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOO157_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:CAULO_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:CAULO_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:BSUB_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:SYNEL_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:PDIF272563_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:CORYNE_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_PWY66-399 gluconeogenesis III
BioCyc:META_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:YEAST_GLUCONEO-YEAST-PWY gluconeogenesis
BioCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:ARA_PWY66-399 gluconeogenesis III
BioCyc:VCHO_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:VCHO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:MTBH37RV_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:HPY_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECOO157_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:ECOO157_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:ECOO157_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOO157_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:CAULO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:CAULO_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:BSUB_GLUCONEO-PWY-1 gluconeogenesis I
BioCyc:BSUB_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:SYNEL_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:PDIF272563_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:ECO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:VCHO_GLUCONEO-PWY gluconeogenesis I
BioCyc:VCHO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:VCHO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:HPY_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOO157_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOO157_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOO157_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:BSUB_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:SYNEL_GLUCONEO-PWY gluconeogenesis I
BioCyc:SYNEL_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:ECOL316407_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:BTHE_GLUCONEO-PWY gluconeogenesis I
BioCyc:CORYNE_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:CORYNE_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:GCF_000013425_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PathBank:SMP0000807 Glycolysis and Pyruvate Dehydrogenase
PathBank:SMP0000839 Gluconeogenesis from L-Malic Acid
PathBank:SMP0000931 Glycerol Metabolism
PathBank:SMP0000932 Glycerol Metabolism II
PathBank:SMP0000933 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000934 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000935 Glycerol Metabolism V (Glycerophosphoserine)
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:PLANT_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:PLANT_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:ARA_PWY66-399 gluconeogenesis III
PlantCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:ARA_GLUCONEO-PWY gluconeogenesis I
PlantCyc:PLANT_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:PLANT_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:ARA_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-DDI-70263 Gluconeogenesis
Reactome:R-HSA-70263 Gluconeogenesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SCE-70326 Glucose metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SSC-71387 Carbohydrate metabolism
Reactome:R-SSC-70326 Glucose metabolism
Reactome:R-DDI-71387 Carbohydrate metabolism
Reactome:R-DDI-70326 Glucose metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-70326 Glucose metabolism
Reactome:R-MTU-936635 Sulfate assimilation
Reactome:R-RNO-70263 Gluconeogenesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-BTA-70326 Glucose metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70326 Glucose metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-70263 Gluconeogenesis
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-SPO-70326 Glucose metabolism
Reactome:R-SSC-70263 Gluconeogenesis
Reactome:R-BTA-70263 Gluconeogenesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-MMU-70263 Gluconeogenesis
Reactome:R-MTU-936621 Sulfur compound metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-70326 Glucose metabolism
Reactome:R-SPO-70263 Gluconeogenesis
PathBank:SMP0121312 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000064 Fructose and Mannose Degradation
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000520 Transaldolase Deficiency
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0087197 Fructose and Mannose Degradation
PathBank:SMP0087246 Pentose Phosphate Pathway
PathBank:SMP0012094 Calvin-Benson Cycle
PathBank:SMP0012445 Fructose Metabolism
PathBank:SMP0121311 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000519 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0000725 Fructose Intolerance, Hereditary
PathBank:SMP0121309 Glycerol Metabolism
PathBank:SMP0121313 Glycerol Metabolism V (Glycerophosphoserine)
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000031 Pentose Phosphate Pathway
PathBank:SMP0000561 Fructosuria
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0087223 Gluconeogenesis
PathBank:SMP0012089 Photosynthesis
PathBank:SMP0121310 Glycerol Metabolism II
PathBank:SMP0000518 Glucose-6-phosphate Dehydrogenase Deficiency
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC