EC: 1.5.1.3

dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant K54Q]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 22
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant K54Q]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 22
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype hDHFR]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 22
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype hDHFR]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl
PH: 7.5
Temperature: 22
uniprot:P0ABQ4 [dihydrofolate reductase(Enzyme) mutant H45R, W47Y, I50F; G51P, insertion of K52]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris, 25 mM Ethanolamine, 100 mM NaCl
PH: 5
Temperature: 25
uniprot:P0ABQ4 [dihydrofolate reductase(Enzyme) mutant H45R, W47Y, I50F]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris, 25 mM Ethanolamine, 100 mM NaCl
PH: 5
Temperature: 25
uniprot:P0ABQ4 [dihydrofolate reductase(Enzyme) mutant H45R, W47Y, I50F; G51P, insertion of K52]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris, 25 mM Ethanolamine, 100 mM NaCl
PH: 8
Temperature: 25
uniprot:P0ABQ4 [dihydrofolate reductase(Enzyme) mutant H45R, W47Y, I50F]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris, 25 mM Ethanolamine, 100 mM NaCl
PH: 8
Temperature: 25
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 37 mM potassium phosphate, 7.4 mM beta-mercaptoethanol
PH: 7.5
Temperature: 25
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * A) ) / (Km + A)) buffer: 37 mM potassium phosphate, 7.4 mM beta-mercaptoethanol
PH: 7.5
Temperature: 25
uniprot:Q920D2 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * B) ) / (Km + B)) buffer: 37 mM potassium phosphate, 7.4 mM beta-mercaptoethanol
PH: 7.5
Temperature: 25
uniprot:P00375 [dihydrofolate reductase(Enzyme) wildtype]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * B) ) / (Km + B)) buffer: 37 mM potassium phosphate, 7.4 mM beta-mercaptoethanol
PH: 7.5
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) mutant W21L]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 7
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

NADP+ + 5,6,7,8_Tetrahydrofolate -> Dihydrofolate + H+ + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 10
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) mutant W21L]

NADP+ + 5,6,7,8_Tetrahydrofolate -> H+ + Dihydrofolate + NADPH		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 10
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Folate + H+ -> Dihydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 7
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 7
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 25 mM Tris(hydroxymethyl)aminomethane, 25 mM Ethanolamine, 500 mM Potassium chloride, 1 mM Ethylenediaminetetraacetate, 1 mM Dithioerythritol
PH: 7
Temperature: 25
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM sodium phosphate
PH: 7
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM sodium acetate
PH: 4.7
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

2 NADPH + Folate + 2 H+ -> 5,6,7,8_Tetrahydrofolate + 2 NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 7
Temperature: 30
uniprot:P07382 [dihydrofolate reductase(Enzyme) wildtype]

Folate + 2 NADPH + 2 H+ -> 5,6,7,8_Tetrahydrofolate + 2 NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium acetate
PH: 5
Temperature: 30
uniprot:P42556 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + (S * (1 + (S / Ki))))) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:P42556 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:P42556 [dihydrofolate reductase(Enzyme) wildtype]

2 NADPH + 2 H+ + Folate -> 5,6,7,8_Tetrahydrofolate + 2 NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:P42556 [dihydrofolate reductase(Enzyme) wildtype]

Folate + 2 H+ + 2 NADPH -> 2 NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

Folate + 2 NADPH + 2 H+ -> 5,6,7,8_Tetrahydrofolate + 2 NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:Q27783 [dihydrofolate reductase(Enzyme) wildtype GST-tagged]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium citrate, 1 mM EDTA, 81 µM cytochrome c, 1% DMSO, 70 µM NaOH
PH: 7.4
Temperature: 36
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

2 NADPH + Folate + 2 H+ -> 2 NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium acetate
PH: 4.7
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

2 H+ + Folate + 2 NADPH -> 2 NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:Q27793 [dihydrofolate reductase(Enzyme) mutant DHFR domain only]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol
PH: 7
Temperature: 25
uniprot:Q27793 [dihydrofolate reductase(Enzyme) mutant DHFR domain only]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol
PH: 7
Temperature: 25
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

2 NADPH + Folate + 2 H+ -> 5,6,7,8_Tetrahydrofolate + 2 NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:P42556 [dihydrofolate reductase(Enzyme) wildtype]

Folate + NADPH + H+ -> NADP+ + Dihydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM K2HPO4/KH2PO4, 1 mM EDTA
PH: 6
Temperature: 22
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
uniprot:Q01782 [dihydrofolate reductase(Enzyme) wildtype]

Folate + 2 NADPH + 2 H+ -> 2 NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM sodium phosphate
PH: 6
Temperature: 30
- [dihydrofolate reductase(Enzyme) mutant R67 Y69L]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype R67]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) mutant R67 T51S]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) mutant R67 V66A]

H+ + NADPH + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) mutant R67 V66T]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) mutant R67 Y46F]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Km_A * Km_B) ) + (Km_A * B)) ) + (Km_B * A)) ) + (A * B))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype R67]

NADH + H+ + Dihydrofolate -> NAD+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (Km_A * B)) ) + (Km_A * Kd1_B)) ) + (Km_B * A)) ) + (( (Km_A * (B ^ 2)) ) / Kd2_B)) ) + (( (Km_B * (A ^ 2)) ) / Kd2_A))) buffer: 100 mM Tris, 50 mM MES, 50 mM acetic acid
PH: 7
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 9 µmoles 2-mercaptoethanol
PH: 6.5
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype]

H+ + NADPH + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 9 µmoles 2-mercaptoethanol
PH: 6.5
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 9 µmoles 2-mercaptoethanol
PH: 6.5
Temperature: 30
- [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 9 µmoles 2-mercaptoethanol
PH: 6.5
Temperature: 30
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22R]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22W]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22Y]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22Y]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22W]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant L22R]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium chloride, 0.02 % sodium azide
PH: 7.65
Temperature: 20
uniprot:P00381 [Carbononitridic bromide]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
uniprot:P00381 [Carbononitridic bromide]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
uniprot:P00381 [Cyanamide]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
uniprot:P00381 [Cyanamide]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype]

H+ + NADPH + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate, 0.1 M KCl
PH: 5.8
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype MTX-resistant]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 1 mg/ml BSA
PH: 7.8
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype MTX-resistant]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 1 mg/ml BSA
PH: 7
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype MTX-resistant]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 1 mg/ml BSA
PH: 7
Temperature: 25
uniprot:P00381 [dihydrofolate reductase(Enzyme) wildtype MTX-resistant]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 1 mg/ml BSA
PH: 7.8
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tes, 75 mM 2-mercaptoethanol, 1 mM EDTA, 1 mg/ml BSA
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N/164L]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N/213A]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N/213A]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N/164L]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant N64S]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35S/N64S]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35S]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35K]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant N64S]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35K]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35S/N64S]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35S]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P13922 [dihydrofolate reductase(Enzyme) wildtype]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P13922 [dihydrofolate reductase(Enzyme) mutant 51I/59R/108N]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM TES, 1 mM EDTA, 75 mM 2-Mercaptoethanol, 1 mg/ml Bovine serum albumin
PH: 7
Temperature: 25
uniprot:P00374 [dihydrofolate reductase(Enzyme) wildtype]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:Q9UUP5 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:Q9UUP5 [dihydrofolate reductase(Enzyme) wildtype]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [dihydrofolate reductase(Enzyme) wildtype]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [Methotrexate]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [Trimethoprim]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [PY957]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35K/N64F]

NADPH + H+ + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant N64F]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant N64F]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35K/N64F]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Methotrexate]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:Q9UUP5 [Methotrexate]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [Trimethoprim]

Dihydrofolate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:Q9UUP5 [Trimethoprim]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [PY957]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:Q9UUP5 [PY957]

Dihydrofolate + NADPH + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant F31R]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium acetate, 0.02% (w/v) sodium azide
PH: 7.6
Temperature: 23
uniprot:P00374 [dihydrofolate reductase(Enzyme) mutant Q35E]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM MES, 25 mM acetate, 50 mM Tris, 100 mM sodium acetate, 0.02% (w/v) sodium azide
PH: 7.6
Temperature: 23
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1+2+3]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1+2+3+4]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H R67]

Dihydrofolate + H+ + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Quad3]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * A) ) / (KmA + A)) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1]

NADPH + Dihydrofolate + H+ -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * A) ) / (KmA + A)) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Quad3]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * B) ) / (KmB + B)) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (kcat * E) ) * B) ) / (KmB + B)) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
- [dihydrofolate reductase(Enzyme) wildtype]

H+ + NADPH + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Phosphate, 2.5 mM 2,3-Dimercaptopropanol
PH: 6.3
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype]

NADPH + H+ + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Phosphate, 2.5 mM 2,3-Dimercaptopropanol
PH: 6.3
Temperature: 25
- [dihydrofolate reductase(Enzyme) wildtype]

Dihydropteroyltriglutamate + NADPH + H+ -> NADP+ + 5,6,7,8_Tetrahydropteroyltriglutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Phosphate, 2.5 mM 2,3-Dimercaptopropanol
PH: 6.3
Temperature: 25
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1+3]

NADPH + Dihydrofolate + H+ -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1+4]

H+ + Dihydrofolate + NADPH -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:P00383 [dihydrofolate reductase(Enzyme) mutant Q67H:1+2]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (kcat * E) ) * A) ) * B) ) / (( (( (( (( (( (A * B) ) + (KmA * B)) ) + (KmA * Kd1B)) ) + (KmB * A)) ) + (( (KmA * (B ^ 2)) ) / Kd2B)) ) + (( (KmB * (A ^ 2)) ) / Kd2A))) buffer: 50 mM MES, 100 mM Tris, 50 mM acetic acid, 10 mM beta-mercaptoethanol
PH: 7
Temperature: 28
uniprot:Q920D2 [Trimethoprim]

7,8_Dihydrobiopterin + H+ + NADPH -> NADP+ + Tetrahydrobiopterin		 (( (( (kcat * E) ) * S1) ) / (( (Km * (1 + (I / Ki))) ) + S1)) buffer: 33 mM Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:Q920D2 [dihydrofolate reductase(Enzyme) wildtype]

H+ + 7,8_Dihydrobiopterin + NADPH -> NADP+ + Tetrahydrobiopterin		 (( (( (kcat * E) ) * S1) ) / (Km + S1)) buffer: 33 mM Potassium phosphate
PH: 6.8
Temperature: 25
uniprot:P16184 [dihydrofolate reductase(Enzyme) mutant K37Q]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [dihydrofolate reductase(Enzyme) mutant K37Q]

H+ + NADPH + Dihydrofolate -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [Trimethoprim]

Dihydrofolate + H+ + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [Methotrexate]

H+ + NADPH + Dihydrofolate -> 5,6,7,8_Tetrahydrofolate + NADP+		 (( (( (( (0 - (( (I + (Ki * (1 + (S / Km)))) ) - E)) ) + (( (( (( (( (I + (Ki * (1 + (S / Km)))) ) - E) ) ^ 2) ) + (( (4 * (Ki * (1 + (S / Km)))) ) * E)) ) ^ 0.5)) ) / (2 * E)) ) * (( (( (E * kcat) ) * S) ) / (Km + S))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37
uniprot:P16184 [PY957]

H+ + Dihydrofolate + NADPH -> NADP+ + 5,6,7,8_Tetrahydrofolate		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + (S * (1 + (I / (alpha * Ki)))))) buffer: 41 mM sodium phosphate, 8.9 mM mercaptoethanol, 150 mM KCl
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:META_PWY-3841 folate transformations II
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY0-1433 tetrahydromonapterin biosynthesis
BioCyc:ECO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-3841 folate transformations II
BioCyc:MTBH37RV_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:BSUB_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:MTBH37RV_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MTBCDC1551_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECOL199310_PWY0-1433 tetrahydromonapterin biosynthesis
BioCyc:BSUB_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:BSUB_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:HUMAN_PWY66-385 dTMP de novo biosynthesis (mitochondrial)
BioCyc:META_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:META_PWY0-1433 tetrahydromonapterin biosynthesis
BioCyc:ECO_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:MTBH37RV_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY0-1433 tetrahydromonapterin biosynthesis
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:YEAST_PWY66-385 dTMP de novo biosynthesis (mitochondrial)
BioCyc:YEAST_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:MTBCDC1551_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MOUSE_PWY-3841 folate transformations II (plants)
BioCyc:BSUB_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECOL316407_1CMET2-PWY formylTHF biosynthesis
PathBank:SMP0000925 Folate Biosynthesis
PathBank:SMP0001750 One Carbon Pool by Folate
PathBank:SMP0002057 Tetrahydromonapterin Biosynthesis
PlantCyc:SOY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:SOY_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:SOY_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:PLANT_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:SOY_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
PlantCyc:SOY_PWY-3841 folate transformations II
PlantCyc:SOY_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:ARA_PWY-6612 superpathway of tetrahydrofolate biosynthesis
PlantCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
PlantCyc:SOY_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-3841 folate transformations II
PlantCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:ARA_PWY-6614 tetrahydrofolate biosynthesis
PlantCyc:PLANT_PWY-3841 folate transformations II
PlantCyc:PLANT_PWY-6612 superpathway of tetrahydrofolate biosynthesis
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
Reactome:R-HSA-69206 G1/S Transition
Reactome:R-HSA-69205 G1/S-Specific Transcription
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196757 Metabolism of folate and pterines
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196757 Metabolism of folate and pterines
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196757 Metabolism of folate and pterines
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-8978934 Metabolism of cofactors
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196757 Metabolism of folate and pterines
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-196757 Metabolism of folate and pterines
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-196757 Metabolism of folate and pterines
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-453279 Mitotic G1 phase and G1/S transition
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-196757 Metabolism of folate and pterines
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
WikiPathways:WP4688 Serine metabolism
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0120742 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0120774 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0000005 Pterine Biosynthesis
PathBank:SMP0000432 Methotrexate Action Pathway
PathBank:SMP0000488 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0002369 One Carbon Pool by Folate I
PathBank:SMP0012014 Folate Biosynthesis
PathBank:SMP0012077 Pyrimidine Deoxyribonucleosides Salvage
PathBank:SMP0120773 Hyperphenylalaninemia Due to 6-Pyruvoyltetrahydropterin Synthase Deficiency (ptps)
PathBank:SMP0000487 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0000491 Sepiapterin Reductase Deficiency
PathBank:SMP0000543 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0120772 Hyperphenylalaninemia Due to Guanosine Triphosphate Cyclohydrolase Deficiency
PathBank:SMP0120776 Sepiapterin Reductase Deficiency
PathBank:SMP0000489 Hyperphenylalaninemia Due to DHPR-Deficiency
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis
PathBank:SMP0120771 DOPA-Responsive Dystonia
PathBank:SMP0120775 Segawa Syndrome
PathBank:SMP0120875 Folate Malabsorption, Hereditary
PathBank:SMP0000053 Folate Metabolism
PathBank:SMP0000486 DOPA-Responsive Dystonia
PathBank:SMP0000490 Segawa Syndrome
PathBank:SMP0000724 Folate Malabsorption, Hereditary
PathBank:SMP0087295 Folate Metabolism
PathBank:SMP0087343 Pterine Biosynthesis