EC: 2.3.2.2

gamma-glutamyltransferase ((5-L-glutamyl)-peptide:amino-acid 5-glutamyltransferase)

enzyme lambda metabolic reaction experiment
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + L_gamma_Glutamyl_p_nitroanilide -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 185 mM Tris-HCl
PH: 8.2
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + L_gamma_Glutamyl_p_nitroanilide -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 185 mM Tris-HCl
PH: 8.2
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + Glycylglycine -> 4_Nitroaniline + L_Glutamylglycylglycine		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (alpha * (Km_D / Ki_A)) ) * (Km_A / Km_DA))) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 100 mM Tris-HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + L_gamma_Glutamyl_p_nitroanilide -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris
PH: 8
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_3_carboxy_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 5_Amino_2_nitrobenzoic_acid		 (( (( (E * Tt) ) * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (( (1 + (D / Ki_D)) ) * (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA))) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (( (( (alpha * Km_D) ) / Ki_A) ) * Km_A) ) / Km_DA)) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 185 mM Tris/HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + L_gamma_Glutamyl_p_nitroanilide -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (( (E * Tt) ) * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (( (( (alpha * Km_D) ) / Ki_A) ) * Km_A) ) / Km_DA)) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 185 mM Tris/HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_3_carboxy_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 5_Amino_2_nitrobenzoic_acid		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (alpha * (Km_D / Ki_A)) ) * (Km_A / Km_DA))) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 100 mM Tris-HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_3_carboxy_p_nitroanilide + Glycylglycine -> 5_Amino_2_nitrobenzoic_acid + L_Glutamylglycylglycine		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (alpha * (Km_D / Ki_A)) ) * (Km_A / Km_DA))) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 100 mM Tris-HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (( (( (1 + (( (alpha * (Km_D / Ki_A)) ) * (Km_A / Km_DA))) ) * D) ) * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2))) ) + (( (Km_D / Ki_A) ) * (A ^ 2)))) buffer: 100 mM Tris-HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (D * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2)))) buffer: 185 mM tris(hydroxymethyl)aminomethane HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_3_carboxy_p_nitroanilide + Glycylglycine -> 5_Amino_2_nitrobenzoic_acid + L_Glutamylglycylglycine		 (( (Vmax * (( (D * A) ) + (( (( (alpha * Km_A) ) / Km_DA) ) * (D ^ 2)))) ) / (( (( (( (( (Km_A + (( (( (alpha * Km_D) ) * Km_A) ) / Km_DA)) ) * D) ) + (Km_D * A)) ) + (D * A)) ) + (( (Km_A / Km_DA) ) * (D ^ 2)))) buffer: 100 mM tris(hydroxymethyl)aminomethane HCl
PH: 8.25
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_alpha_Methyl_gamma_glutamyl_L_alpha_aminobutyrate + L_Methionine -> L_alpha_Aminobutyrate + L_alpha_Methyl_gamma_glutamylmethionine		 (( (( (Vab * A) ) * B) ) / (( (( (( (Kah * Kbt) ) + (Kat * B)) ) + (Kbt * A)) ) + (A * B))) buffer: 0.1 M Tris-HCl
PH: 9
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_alpha_Methyl_gamma_glutamyl_L_alpha_aminobutyrate + L_Methionine -> L_alpha_Methyl_gamma_glutamylmethionine + L_alpha_Aminobutyrate		 (( (Va * A) ) / (Kah + (( (A * (1 + (B / Kbt))) ) / (1 + (B / Kibb))))) buffer: 0.1 M Tris-HCl
PH: 9
Temperature: 37
uniprot:Q4ZH57 [ADP]

L_Glutamine + Hydroxylamine -> NH3 + L_gamma_Glutamyl_hydroxamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 135 mM Imidazole-HCl, 25 mM K-arsenate
PH: 6.4
Temperature: 37
uniprot:Q4ZH57 [ADP]

Hydroxylamine + L_Glutamine -> L_gamma_Glutamyl_hydroxamate + NH3		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 135 mM Imidazole-HCl, 25 mM K-arsenate
PH: 6.4
Temperature: 37
uniprot:Q4ZH57 [ADP]

L_Glutamine + Hydroxylamine -> NH3 + L_gamma_Glutamyl_hydroxamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 135 mM Imidazole-HCl, 25 mM K-arsenate
PH: 6.4
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

Reduced_glutathione + H2O -> L_Glutamate + L_Cysteinylglycine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 8.73
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype isoform I]

1_Aminocyclopropane_1_carboxylate + Reduced_glutathione -> 1_(gamma_Glutamyl)cyclopropane_1_carboxylic_acid + L_Cysteinylglycine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-Cl
PH: 8
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype isoform II]

Reduced_glutathione + 1_Aminocyclopropane_1_carboxylate -> 1_(gamma_Glutamyl)cyclopropane_1_carboxylic_acid + L_Cysteinylglycine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-Cl
PH: 8
Temperature: 30
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + H2O -> L_Glutamate + 4_Nitroaniline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 8.73
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_Arginine + L_gamma_Glutamyl_p_nitroanilide -> L_gamma_Glutamyl_L_arginine + 4_Nitroaniline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 8.73
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + L_gamma_Glutamyl_p_nitroanilide -> 4_Nitroaniline + L_Glutamylglycylglycine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 9
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

L_gamma_Glutamyl_p_nitroanilide + Glycylglycine -> L_Glutamylglycylglycine + 4_Nitroaniline		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 9
Temperature: 37
- [gamma-glutamyltransferase(Enzyme) wildtype]

Glycylglycine + Reduced_glutathione -> L_Cysteinylglycine + L_Glutamylglycylglycine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-hydrochloride
PH: 9
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-4061 glutathione-mediated detoxification I
BioCyc:META_PWY-6842 glutathione-mediated detoxification II
BioCyc:YEAST_PWY-6842-1 glutathione-mediated detoxification II
BioCyc:HUMAN_PWY66-375 leukotriene biosynthesis
BioCyc:HUMAN_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-4061 glutathione-mediated detoxification I
BioCyc:YEAST_PWY-7559 glutathione degradation (DUG pathway)
BioCyc:ARA_PWY-6842 glutathione-mediated detoxification II
BioCyc:ARA_PWY-4041 γ-glutamyl cycle
BioCyc:MOUSE_PWY-4041 γ-glutamyl cycle
BioCyc:ARA_PWYQT-4432 glutathione degradation
BioCyc:META_PWY66-375 leukotriene biosynthesis
BioCyc:META_PWY-7559 glutathione degradation (DUG pathway - yeast)
BioCyc:ARA_PWY-6745 phytochelatins biosynthesis
PlantCyc:ARA_PWY-4041 γ-glutamyl cycle
PlantCyc:ARA_PWY-6842 glutathione-mediated detoxification II
PlantCyc:ARA_PWY-6745 phytochelatins biosynthesis
PlantCyc:PLANT_PWYQT-4432 glutathione degradation
PlantCyc:PLANT_PWY-4041 γ-glutamyl cycle
PlantCyc:PLANT_PWY-6842 glutathione-mediated detoxification II
PlantCyc:ARA_PWYQT-4432 glutathione degradation
Reactome:R-BTA-9748784 Drug ADME
Reactome:R-MMU-9753281 APAP ADME
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-SCE-9748784 Drug ADME
Reactome:R-BTA-9753281 APAP ADME
Reactome:R-RNO-9753281 APAP ADME
Reactome:R-SCE-9753281 APAP ADME
Reactome:R-SPO-9748784 Drug ADME
Reactome:R-HSA-9658195 Leishmania infection
Reactome:R-HSA-9664433 Leishmania parasite growth and survival
Reactome:R-HSA-9753281 APAP ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-HSA-9662851 Anti-inflammatory response favouring Leishmania parasite infection
Reactome:R-HSA-9664535 LTC4-CYSLTR mediated IL4 production
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-SPO-9753281 APAP ADME
Reactome:R-HSA-9824443 Parasitic Infection Pathways
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-156590 Glutathione conjugation
Reactome:R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-5423646 Aflatoxin activation and detoxification
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-5423646 Aflatoxin activation and detoxification
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-156590 Glutathione conjugation
Reactome:R-SCE-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-SPO-5423646 Aflatoxin activation and detoxification
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-BTA-5423646 Aflatoxin activation and detoxification
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-2142753 Arachidonate metabolism
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-RNO-5423646 Aflatoxin activation and detoxification
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-8978868 Fatty acid metabolism
Reactome:R-SCE-174403 Glutathione synthesis and recycling
Reactome:R-SPO-8978868 Fatty acid metabolism
Reactome:R-SPO-211859 Biological oxidations
Reactome:R-SPO-174403 Glutathione synthesis and recycling
Reactome:R-BTA-174403 Glutathione synthesis and recycling
Reactome:R-HSA-156590 Glutathione conjugation
Reactome:R-MMU-156590 Glutathione conjugation
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-RNO-174403 Glutathione synthesis and recycling
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-156580 Phase II - Conjugation of compounds
Reactome:R-SCE-5423646 Aflatoxin activation and detoxification
Reactome:R-SPO-156580 Phase II - Conjugation of compounds
Reactome:R-BTA-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-174403 Glutathione synthesis and recycling
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-2142753 Arachidonate metabolism
Reactome:R-MMU-174403 Glutathione synthesis and recycling
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
Reactome:R-SCE-2142753 Arachidonate metabolism
Reactome:R-SCE-211859 Biological oxidations
Reactome:R-SCE-156590 Glutathione conjugation
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-2142753 Arachidonate metabolism
Reactome:R-SPO-156590 Glutathione conjugation
WikiPathways:WP4518 Gamma-glutamyl cycle for the biosynthesis and degradation of glutathione, including diseases
PathBank:SMP0120480 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120565 5-Oxoprolinase Deficiency
PathBank:SMP0121212 Camalexin Biosynthesis
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0120705 Glutathione Synthetase Deficiency
PathBank:SMP0120785 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0002317 Glutathione Metabolism
PathBank:SMP0087277 Glutathione Metabolism
PathBank:SMP0087309 Taurine and Hypotaurine Metabolism
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0120700 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120784 5-Oxoprolinase Deficiency
PathBank:SMP0120485 Glutathione Synthetase Deficiency
PathBank:SMP0120566 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0087167 Glutathione Metabolism
PathBank:SMP0120667 5-Oxoprolinuria
PathBank:SMP0120446 5-Oxoprolinuria
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000029 Selenoamino Acid Metabolism
PathBank:SMP0000021 Taurine and Hypotaurine Metabolism
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0087214 Taurine and Hypotaurine Metabolism
PathBank:SMP0063622 Glutathione Metabolism
PathBank:SMP0063675 Taurine and Hypotaurine Metabolism