EC: 1.3.3.6

acyl-CoA oxidase (acyl-CoA:oxygen 2-oxidoreductase)

enzyme lambda metabolic reaction experiment
uniprot:Q15067 [acyl-CoA oxidase(Enzyme) wildtype his-tagged]

O2 + Palmitoyl_CoA -> trans_Hexadec_2_enoyl_CoA + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 0.82 mM 4-aminoantipyrine, 10.6 mM phenol, 10 µM FAD, 5IU/ml horseradish peroxidase
PH: 7.4
Temperature: 30
- [FAD]

O2 + Palmitoyl_CoA -> H2O2 + trans_Hexadec_2_enoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Lauroyl_CoA + O2 -> H2O2 + 2_trans_Dodecenoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Myristoyl_CoA + O2 -> H2O2 + trans_Tetradec_2_enoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Octanoyl_CoA + O2 -> trans_Oct_2_enoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Butanoyl_CoA + O2 -> Crotonoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Decanoyl_CoA + O2 -> trans_Dec_2_enoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

O2 + Stearoyl_CoA -> trans_2_Octadecenoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

Oleoyl_CoA + O2 -> H2O2 + 2_trans_9_trans_Octadecendienoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

O2 + Eicosanoyl_CoA -> trans_Eicos_2_enoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [FAD]

O2 + Hexanoyl_CoA -> H2O2 + trans_Hex_2_enoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate, 0.825 mM 4-aminoantipyrine, 10.6 mM phenol, 30 U/ml peroxidase
PH: 7.4
Temperature: 30
- [acyl-CoA oxidase(Enzyme) wildtype]

O2 + Palmitoyl_CoA -> H2O2 + trans_Hexadec_2_enoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate
PH: 7.4
Temperature: 30
- [acyl-CoA oxidase(Enzyme) wildtype]

Lauroyl_CoA + O2 -> 2_trans_Dodecenoyl_CoA + H2O2		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate
PH: 7.4
Temperature: 30
- [acyl-CoA oxidase(Enzyme) wildtype]

Myristoyl_CoA + O2 -> H2O2 + trans_Tetradec_2_enoyl_CoA		 (( (Vmax * A) ) / (A + Km)) buffer: 50 mM potassium phosphate
PH: 7.4
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-7338 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast)
BioCyc:YEAST_PWY-6837 fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
BioCyc:YEAST_PWY-7288 fatty acid β-oxidation (peroxisome)
BioCyc:YEAST_PWY-7291 oleate β-oxidation (isomerase-dependent)
BioCyc:ARA_PWY-6837 fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
BioCyc:META_PWY-7726 (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)
BioCyc:META_PWY-6837 fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
BioCyc:META_PWY-7340 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast)
BioCyc:META_PWY-7337 10-cis-heptadecenoyl-CoA degradation (yeast)
BioCyc:YEAST_PWY-7338 10-trans-heptadecenoyl-CoA degradation (reductase-dependent)
BioCyc:META_PWY-7288 fatty acid β-oxidation (peroxisome, yeast)
BioCyc:META_PWY-7291 oleate β-oxidation (isomerase-dependent, yeast)
BioCyc:META_PWY-735 jasmonic acid biosynthesis
BioCyc:YEAST_PWY-7337 10-cis-heptadecenoyl-CoA degradation
BioCyc:HUMAN_PWY66-391 fatty acid β-oxidation (peroxisome)
BioCyc:META_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
BioCyc:META_PWY-6920 6-gingerol analog biosynthesis (engineered)
BioCyc:META_PWY-5136 fatty acid β-oxidation II (peroxisome)
BioCyc:META_PWY66-391 fatty acid β-oxidation VI (peroxisome)
BioCyc:META_PWY-7606 docosahexaenoate biosynthesis III (6-desaturase, mammals)
BioCyc:YEAST_YEAST-FAO-PWY fatty acid oxidation (non-cyclic)
BioCyc:YEAST_PWY-7340 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent)
PlantCyc:PLANT_PWY-5136 fatty acid β-oxidation II (peroxisome)
PlantCyc:ARA_PWY-6837 fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
PlantCyc:ARA_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
PlantCyc:ARA_PWY-5136 fatty acid β-oxidation II (peroxisome)
PlantCyc:ARA_PWY-735 jasmonic acid biosynthesis
PlantCyc:PLANT_PWY-6837 fatty acid beta-oxidation V (unsaturated, odd number, di-isomerase-dependent)
PlantCyc:PLANT_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
PlantCyc:PLANT_PWY-735 jasmonic acid biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-DDI-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-DDI-390918 Peroxisomal lipid metabolism
Reactome:R-DDI-192105 Synthesis of bile acids and bile salts
Reactome:R-DME-390918 Peroxisomal lipid metabolism
Reactome:R-HSA-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-HSA-9033500 TYSND1 cleaves peroxisomal proteins
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-MMU-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-DDI-8978868 Fatty acid metabolism
Reactome:R-DDI-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-DDI-194068 Bile acid and bile salt metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-DME-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-9033241 Peroxisomal protein import
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-RNO-390918 Peroxisomal lipid metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-8978868 Fatty acid metabolism
Reactome:R-SCE-390918 Peroxisomal lipid metabolism
Reactome:R-BTA-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-DDI-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-DDI-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
Reactome:R-DME-8978868 Fatty acid metabolism
Reactome:R-HSA-9609507 Protein localization
Reactome:R-MMU-390247 Beta-oxidation of very long chain fatty acids
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-2046106 alpha-linolenic acid (ALA) metabolism
Reactome:R-RNO-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-BTA-390918 Peroxisomal lipid metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-DDI-389887 Beta-oxidation of pristanoyl-CoA
Reactome:R-DME-556833 Metabolism of lipids
Reactome:R-DME-2046104 alpha-linolenic (omega3) and linoleic (omega6) acid metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-390918 Peroxisomal lipid metabolism
Reactome:R-MMU-390918 Peroxisomal lipid metabolism
Reactome:R-RNO-390247 Beta-oxidation of very long chain fatty acids
WikiPathways:WP1941 Peroxisomal beta-oxidation of tetracosanoyl-CoA
PathBank:SMP0121206 Jasmonic Acid Biosynthesis
PathBank:SMP0012060 Fatty Acid Beta-Oxidation II
PathBank:SMP0087236 Bile Acid Biosynthesis