EC: 1.17.4.1

ribonucleoside-diphosphate reductase (2'-deoxyribonucleoside-5'-diphosphate:thioredoxin-disulfide 2'-oxidoreductase)

enzyme lambda metabolic reaction experiment
- [dATP]

1,4_Dithiothreitol + CTP -> Oxidized_dithiothreitol + dCTP + H2O		 (( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 1 mM EDTA, 0.01 mM adenosylcobalamin, 10 mM CaCl2
PH: 8.2
Temperature: 35
- [ribonucleoside-diphosphate reductase(Enzyme) wildtype]

CDP + Reduced_glutaredoxin -> dCDP + H2O + Oxidized_glutaredoxin		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 4 mM GSH, 1 mM NADPH, excess GSH reductase
PH: 7.6
Temperature: 37
- [ribonucleoside-diphosphate reductase(Enzyme) wildtype]

Reduced_glutaredoxin + CDP -> H2O + dCDP + Oxidized_glutaredoxin		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 4 mM GSH, 1 mM NADPH, excess GSH reductase
PH: 7.6
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:META_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:META_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ARA_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBH37RV_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis I
BioCyc:AGRO_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOL316407_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7228 guanosine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:HUMAN_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:META_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:YEAST_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:YEAST_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBCDC1551_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:AGRO_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:META_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:AGRO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7210 pyrimidine deoxyribonucleotides biosynthesis from CTP
BioCyc:YEAST_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ARA_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBH37RV_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:AGRO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0000959 Pyrimidine Metabolism
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
PlantCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7226 guanosine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
PlantCyc:ARA_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
PlantCyc:PLANT_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7227 adenosine deoxyribonucleotides de novo biosynthesis
Reactome:R-HSA-9645723 Diseases of programmed cell death
Reactome:R-HSA-9693928 Defective RIPK1-mediated regulated necrosis
Reactome:R-HSA-9686347 Microbial modulation of RIPK1-mediated regulated necrosis
Reactome:R-HSA-69206 G1/S Transition
Reactome:R-HSA-69205 G1/S-Specific Transcription
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-74160 Gene expression (Transcription)
Reactome:R-HSA-73857 RNA Polymerase II Transcription
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-HSA-5213460 RIPK1-mediated regulated necrosis
Reactome:R-HSA-1640170 Cell Cycle
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-CEL-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DME-15869 Nucleotide metabolism
Reactome:R-DME-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-5357801 Programmed Cell Death
Reactome:R-HSA-69278 Cell Cycle, Mitotic
Reactome:R-HSA-212436 Generic Transcription Pathway
Reactome:R-HSA-3700989 Transcriptional Regulation by TP53
Reactome:R-HSA-8953750 Transcriptional Regulation by E2F6
Reactome:R-RNO-1430728 Metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-HSA-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-HSA-5218859 Regulated Necrosis
Reactome:R-HSA-453279 Mitotic G1 phase and G1/S transition
Reactome:R-HSA-5628897 TP53 Regulates Metabolic Genes
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-499943 Interconversion of nucleotide di- and triphosphates
WikiPathways:WP4584 Biomarkers for pyrimidine metabolism disorders
WikiPathways:WP4225 Pyrimidine metabolism and related diseases
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120678 beta-Ureidopropionase Deficiency
PathBank:SMP0120750 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120693 Dihydropyrimidinase Deficiency
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0087473 Pyrimidine Metabolism
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0002370 Pyrimidine Metabolism
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0087340 Pyrimidine Metabolism
PathBank:SMP0120727 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0000603 Gemcitabine Metabolism Pathway