EC: 6.3.4.3

formate-tetrahydrofolate ligase (formate:tetrahydrofolate ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q83WS0 [formate---tetrahydrofolate ligase(Enzyme) wildtype]

Formate + ATP + 5,6,7,8_Tetrahydrofolate -> 10_Formyltetrahydrofolate + ADP + Phosphate		 (( (Vmax * S1) ) / (Km1 + S1)) buffer: 0.1 M Tris, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:Q83WS0 [formate---tetrahydrofolate ligase(Enzyme) wildtype]

5,6,7,8_Tetrahydrofolate + Formate + ATP -> ADP + 10_Formyltetrahydrofolate + Phosphate		 (( (Vmax * S3) ) / (Km3 + S3)) buffer: 0.1 M Tris, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:Q83WS0 [formate---tetrahydrofolate ligase(Enzyme) wildtype]

5,6,7,8_Tetrahydrofolate + ATP + Formate -> ADP + Phosphate + 10_Formyltetrahydrofolate		 (( (Vmax * S2) ) / (Km2 + S2)) buffer: 0.1 M Tris, 10 mM MgCl2
PH: 8
Temperature: 30
uniprot:Q4Q735 [formate---tetrahydrofolate ligase(Enzyme) wildtype His6-tagged]

5,6,7,8_Tetrahydrofolate + ATP + Formate -> Phosphate + 10_Formyltetrahydrofolate + ADP		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM K+-HEPES, 100 mM KCl, 10 mM MgSO4
PH: 7.5
Temperature: 30
uniprot:Q4Q735 [formate---tetrahydrofolate ligase(Enzyme) wildtype His6-tagged]

5,6,7,8_Tetrahydrofolate + ATP + Formate -> 10_Formyltetrahydrofolate + ADP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM K+-HEPES, 100 mM KCl, 10 mM MgSO4
PH: 7.5
Temperature: 30

Pathways

pathway id name
BioCyc:HUMAN_PWY-2161 folate polyglutamylation
BioCyc:META_CODH-PWY reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)
BioCyc:META_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:META_PWY-2201 folate transformations I
BioCyc:META_PWY-5030 L-histidine degradation III
BioCyc:YEAST_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ARA_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:ARA_PWY-3841 folate transformations II
BioCyc:ARA_PWY-2161 folate polyglutamylation
BioCyc:ANTHRA_PWY-2161 folate polyglutamylation
BioCyc:PDIF272563_CODH-PWY reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)
BioCyc:BTHE_PWY-2201 folate transformations I
BioCyc:HUMAN_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:META_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:YEAST_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:YEAST_PWY3O-697 folate interconversions
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:ANTHRA_1CMET2-PWY N10-formyl-tetrahydrofolate biosynthesis
BioCyc:MOUSE_PWY-2161 folate polyglutamylation
BioCyc:MOUSE_PWY-2201 folate transformations I
BioCyc:PDIF272563_PWY-2161 folate polyglutamylation
BioCyc:GCF_000013425_PWY-2201 folate transformations I
BioCyc:HUMAN_PWY-5030 histidine degradation
BioCyc:YEAST_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:YEAST_PWY-2161 folate polyglutamylation
BioCyc:MOUSE_PWY-5030 histidine degradation III
BioCyc:MOUSE_PWY-6613 tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate
BioCyc:GCF_000013425_PWY-2161 folate polyglutamylation
BioCyc:HUMAN_PWY-2201 folate transformations I
BioCyc:YEAST_PWY-2201 folate transformations
BioCyc:ANTHRA_PWY-2201 folate transformations I
BioCyc:MOUSE_PWY-3841 folate transformations II (plants)
BioCyc:PDIF272563_PWY-2201 folate transformations I
BioCyc:PDIF272563_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
BioCyc:BTHE_PWY-2161 folate polyglutamylation
BioCyc:BTHE_PWY-1722 formate reduction to 5,10-methylenetetrahydrofolate
BioCyc:GCF_000013425_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
PlantCyc:PLANT_PWY-1722 formate assimilation into 5,10-methylenetetrahydrofolate
PlantCyc:ARA_PWY-2161 folate polyglutamylation
PlantCyc:PLANT_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-3841 folate transformations II
PlantCyc:PLANT_PWY-3841 folate transformations II
PlantCyc:PLANT_PWY-2161 folate polyglutamylation
Reactome:R-BTA-1430728 Metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-196757 Metabolism of folate and pterines
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196757 Metabolism of folate and pterines
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196757 Metabolism of folate and pterines
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196757 Metabolism of folate and pterines
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196757 Metabolism of folate and pterines
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-196757 Metabolism of folate and pterines
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-196757 Metabolism of folate and pterines
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
WikiPathways:WP4259 Disorders of folate metabolism and transport
PathBank:SMP0000432 Methotrexate Action Pathway
PathBank:SMP0000543 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0002323 Serine Metabolism
PathBank:SMP0120656 Folate Malabsorption, Hereditary
PathBank:SMP0002320 Glycine Metabolism
PathBank:SMP0002334 Tetrahydrofolate Biosynthesis
PathBank:SMP0120522 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000053 Folate Metabolism
PathBank:SMP0000724 Folate Malabsorption, Hereditary
PathBank:SMP0000801 Folate Metabolism
PathBank:SMP0012063 Folate Polyglutamylation