EC: 5.1.3.3

Aldose 1-epimerase (Aldose 1-epimerase)

enzyme lambda metabolic reaction experiment
uniprot:P0A9C3 [H+]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (E * kcat) ) * S) ) / (( (kcat / (c / (( (1 + (H / Ka1)) ) + (Ka2 / (H ^ 2))))) ) + S)) buffer: 50 mM acetate (pH 4.0 - 5.5), 50 mM N-(2-acetamido)-2-iminodiacetic acid (pH 6.0 - 6.5), 50 mM HEPES (pH 7.0 - 8.0), 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid (pH 8.5)
PH: 4
Temperature: 27
uniprot:P0A9C3 [H+]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (E * kcat) ) * S) ) / (( (kcat / (c / (( (1 + (( (10 ^ (0 - pH)) ) / (10 ^ (0 - pKa1)))) ) + (( (10 ^ (0 - pKa2)) ) / (10 ^ (0 - pH)))))) ) + S)) buffer: 50 mM acetate (pH 4.0 - 5.5), 50 mM N-(2-acetamido)-2-iminodiacetic acid (pH 6.0 - 6.5), 50 mM HEPES (pH 7.0 - 8.0), 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid (pH 8.5)
PH: 4
Temperature: 27
uniprot:P0A9C3 [H+]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (E * kcat) ) * S) ) / (( (kcat / (c / (1 + (H / Ka)))) ) + S)) buffer: 50 mM acetate (pH 4.0 - 5.5), 50 mM N-(2-acetamido)-2-iminodiacetic acid (pH 6.0 - 6.5), 50 mM HEPES (pH 7.0 - 8.0), 50 mM N-(2-hydroxyethyl)piperazine-N'-3-propanesulfonic acid (pH 8.5)
PH: 4
Temperature: 27
uniprot:P0A9C3 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 27
uniprot:P0A9C3 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Galactose -> beta_D_Galactose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 27
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM HEPES-NaOH, 50 mM NaCl
PH: 8
Temperature: 20
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) mutant H107A]

alpha_D_Glucose -> beta_D_Glucose		 (( (kcat_Km * E) ) * S) buffer: 10 mM HEPES-NaOH, 3 mM NAD+, 30 U/ml glucose dehydrogenase
PH: 8
Temperature: 27
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Glucose -> beta_D_Glucose		 (( (kcat_Km * E) ) * S) buffer: 10 mM HEPES-NaOH, 3 mM NAD+
PH: 8
Temperature: 27
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Galactose -> beta_D_Galactose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM HEPES-NaOH, 50 mM NaCl
PH: 8
Temperature: 20
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Galactose -> beta_D_Galactose		 (( (kcat_Km * E) ) * S) buffer: 10 mM HEPES-NaOH, 3 mM NAD+
PH: 8
Temperature: 27
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) mutant H107A]

alpha_D_Galactose -> beta_D_Galactose		 (( (kcat_Km * E) ) * S) buffer: 10 mM HEPES-NaOH, 3 mM NAD+, 30 U/ml galactose dehydrogenase
PH: 8
Temperature: 27
uniprot:Q96C23 [aldose 1-epimerase(Enzyme) mutant H176A]

alpha_D_Galactose -> beta_D_Galactose		 (( (kcat_Km * E) ) * S) buffer: 10 mM HEPES-NaOH, 3 mM NAD+, 30 U/ml galactose dehydrogenase
PH: 8
Temperature: 27
- [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Glucose -> beta_D_Glucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25
- [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Galactose -> beta_D_Galactose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25
- [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Quinovopyranose -> beta_D_Quinovose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25
- [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Xylose -> beta_D_Xylose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25
- [aldose 1-epimerase(Enzyme) wildtype]

beta_L_Arabinose -> alpha_L_Arabinose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25
- [aldose 1-epimerase(Enzyme) wildtype]

alpha_D_Fucose -> beta_D_Fucose		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Hepes, 50 mM KCl
PH: 7.6
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:META_PWY-6317 D-galactose degradation I (Leloir pathway)
BioCyc:MOUSE_PWY66-373 sucrose degradation V (mammalian)
BioCyc:MOUSE_PWY-6317 galactose degradation I (Leloir pathway)
BioCyc:ECOL316407_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:HUMAN_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:YEAST_PWY66-422 D-galactose degradation V (Leloir pathway)
BioCyc:YEAST_PWY-7344 UDP-D-galactose biosynthesis
BioCyc:ECO_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
PathBank:SMP0000841 Galactose Metabolism
PathBank:SMP0000903 Galactose Degradation/Leloir Pathway
Reactome:R-BTA-1430728 Metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-70370 Galactose catabolism
Reactome:R-SSC-71387 Carbohydrate metabolism
Reactome:R-SSC-70370 Galactose catabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70370 Galactose catabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-BTA-70370 Galactose catabolism
Reactome:R-HSA-70370 Galactose catabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70370 Galactose catabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-70370 Galactose catabolism
PathBank:SMP0120846 Triosephosphate Isomerase Deficiency
PathBank:SMP0120488 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0120637 Glycogenosis, Type IB
PathBank:SMP0120645 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0000572 Fanconi-Bickel Syndrome
PathBank:SMP0002319 Leloir Pathway
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0087318 Gluconeogenesis
PathBank:SMP0120845 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0120857 Glycogenosis, Type IC
PathBank:SMP0120596 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0120624 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0120636 Fanconi-Bickel Syndrome
PathBank:SMP0000040 Glycolysis
PathBank:SMP0000531 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0087317 Glycolysis
PathBank:SMP0063617 Gluconeogenesis
PathBank:SMP0120708 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0120856 Glycogenosis, Type IB
PathBank:SMP0120864 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0120626 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0120638 Glycogenosis, Type IC
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0063478 Glycolysis
PathBank:SMP0120815 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0120843 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0120855 Fanconi-Bickel Syndrome
PathBank:SMP0120627 Triosephosphate Isomerase Deficiency
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC