uniprot:Q2PYM9 [lactoylglutathione lyase(Enzyme) wildtype GLO I]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M potassium phosphate
PH: 6.8
Temperature: 30 |
uniprot:Q2PYM9 [lactoylglutathione lyase(Enzyme) wildtype GLO I]
Trypanothione_methylglyoxal_hemithioacetal -> (R)_S_Lactoyltrypanothione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M potassium phosphate
PH: 6.8
Temperature: 30 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 3_Methoxyphenylglyoxal -> 3_Methoxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 3_Methoxyphenylglyoxal -> 3_Methoxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Methoxyphenylglyoxal -> 4_Methoxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Phenylphenylglyoxal -> 4_Phenyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Methoxyphenylglyoxal -> 4_Methoxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Bromophenylglyoxal -> 4_Bromo_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Methylphenylglyoxal -> 4_Methyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 2,4_Dimethylphenylglyoxal -> 2,4_Dimethyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
2,4_Dimethylphenylglyoxal + Reduced_glutathione -> 2,4_Dimethyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Methylglyoxal + Reduced_glutathione -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + Phenylglyoxal -> S_Mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + p_Chlorophenylglyoxal -> p_Chloro_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + Kethoxal -> 3_Ethoxy_2_hydroxybutanoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + Methylglyoxal -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + Phenylglyoxal -> S_Mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + p_Chlorophenylglyoxal -> p_Chloro_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
p_Hydroxyphenylglyoxal + Reduced_glutathione -> p_Hydroxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Kethoxal + Reduced_glutathione -> 3_Ethoxy_2_hydroxybutanoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
4_Phenylphenylglyoxal + Reduced_glutathione -> 4_Phenyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + 4_Bromophenylglyoxal -> 4_Bromo_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
4_Methylphenylglyoxal + Reduced_glutathione -> 4_Methyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.2 M Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:Q6P7Q4 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + p_Hydroxyphenylglyoxal -> p_Hydroxy_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (Vmax / Kms) ) * (S - (P / Keq))) ) / (( (1 + (S / Kms)) ) + (P / Kmp))) |
|
buffer: 0.1 M Mes/NaOH
PH: 6.5
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Mes/NaOH
PH: 6.5
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Mes/NaOH
PH: 6.5
Temperature: 30 |
uniprot:Q68RJ8 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_ethyl_ester_methylglyoxal_hemithioacetal -> S_Lactoylglutathionyl_ethyl_ester |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [S-2,4-Dinitrophenylglutathionylspermidine]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_isopropyl_ester_methylglyoxal_hemithioacetal -> S_Lactoylglutathionyl_isopropyl_ester |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Trypanothione_methylglyoxal_hemithioacetal -> (R)_S_Lactoyltrypanothione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_isopropyl_ester_methylglyoxal_hemithioacetal -> S_Lactoylglutathionyl_isopropyl_ester |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [lactoylglutathione lyase(Enzyme) wildtype]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [lactoylglutathione lyase(Enzyme) wildtype]
Trypanothione_methylglyoxal_hemithioacetal -> (R)_S_Lactoyltrypanothione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:Q04760 [S-(2,4-Dinitrophenyl)glutathione]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [S-4-Bromobenzylglutathione]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_ethyl_ester_methylglyoxal_hemithioacetal -> S_Lactoylglutathionyl_ethyl_ester |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [S-2,4-Dinitrophenylglutathionylspermidine]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q04760 [S-4-Bromobenzylglutathionylspermidine]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate
PH: 7
Temperature: 27 |
uniprot:Q68RJ8 [S-4-Bromobenzylglutathionylspermidine]
N1_Glutathionylspermidine_methylglyoxal_hemithioacetal -> S_Lactoylglutathionylspermidine |
(( (( (kcat * E) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) |
|
buffer: 100 mM Na-Phosphate, 20 µM NiCl2, 0.05% (w/v) BSA
PH: 7
Temperature: 27 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) mutant E163Q]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) mutant E318Q]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) mutant E163Q, E318Q]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M sodium phosphate, 0.1 mM free glutathione
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157Q]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157A]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157H]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157Q]
Glutathione_phenylglyoxal_hemithioacetal -> (R)_S_Mandeloylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157A]
Glutathione_phenylglyoxal_hemithioacetal -> (R)_S_Mandeloylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) mutant M157H]
Glutathione_phenylglyoxal_hemithioacetal -> (R)_S_Mandeloylglutathione |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M Sodium phosphate
PH: 7
Temperature: 30 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Methylglyoxal + Reduced_glutathione -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P0AC81 [lactoylglutathione lyase(Enzyme) wildtype]
Methylglyoxal + Reduced_glutathione -> (R)_S_Lactoylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Phenylglyoxal + Reduced_glutathione -> S_Mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P50107 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + p_Chlorophenylglyoxal -> p_Chloro_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P0AC81 [lactoylglutathione lyase(Enzyme) wildtype]
2,4,6_Trimethylphenylglyoxal + Reduced_glutathione -> 2,4,6_Trimethyl_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P0AC81 [lactoylglutathione lyase(Enzyme) wildtype]
Phenylglyoxal + Reduced_glutathione -> S_Mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
uniprot:P0AC81 [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + p_Chlorophenylglyoxal -> p_Chloro_S_mandelylglutathione |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: Phosphate, 10 mM MgCl2
PH: 7
Temperature: 25 |
- [lactoylglutathione lyase(Enzyme) wildtype]
Methylglyoxal + Reduced_glutathione -> (R)_S_Lactoylglutathione |
(( (( (kcat * E) ) * S1) ) / (Km + S1)) |
|
buffer: 0.05 M Sodium phosphate
PH: 6.6
Temperature: 36 |
- [lactoylglutathione lyase(Enzyme) wildtype]
Reduced_glutathione + Phenylglyoxal -> S_Mandelylglutathione |
(( (( (kcat * E) ) * S1) ) / (Km + S1)) |
|
buffer: 0.05 M Sodium phosphate
PH: 6.6
Temperature: 36 |
uniprot:Q04760 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM potassium phosphate, 100 mM KCl
PH: 7
Temperature: 30 |
uniprot:Q71KM3 [lactoylglutathione lyase(Enzyme) wildtype]
Glutathione_methylglyoxal_hemithioacetal -> (R)_S_Lactoylglutathione |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM potassium phosphate, 100 mM KCl
PH: 7
Temperature: 30 |