EC: 2.8.1.7

cysteine desulfurase (L-cysteine:acceptor sulfurtransferase)

enzyme lambda metabolic reaction experiment
uniprot:P0A6B7 [cysteine desulfurase(Enzyme) wildtype]

L_Cysteine -> L_Alanine + Sulfide		 (( (vmax * s0) ) / (km_s0 + s0)) buffer:
PH: 7.5
Temperature: 37
- [Alkylated protein]

Acceptor + L_Cysteine -> S_sulfanyl_acceptor + L_Alanine		 (Vmax * (( (1 + (I / IC_50)) ) ^ nH)) buffer: 50 mM Mops, 0.5-2 mM Dithiothreitol
PH: 7.4
Temperature: 36
uniprot:Q93WX6 [CpSufE]

Acceptor + L_Cysteine -> S_sulfanyl_acceptor + L_Alanine		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Tris-HCl, 100 mM NaCl, 1 mM Dithiothreitol
PH: 7.4
Temperature: 25
uniprot:Q93WX6 [Pyridoxal phosphate]

L_Cysteine + Acceptor -> L_Alanine + S_sulfanyl_acceptor		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Tris-HCl, 100 mM NaCl, 1 mM Dithiothreitol
PH: 7.4
Temperature: 25
uniprot:O49543 [Frataxin]

L_Cysteine + Acceptor -> S_sulfanyl_acceptor + L_Alanine		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 25 mM Tris-HCl, 100 mM NaCl, 100 µM DTT
PH: 8
Temperature: 37
uniprot:O49543 [Pyridoxal phosphate]

L_Cysteine + Acceptor -> S_sulfanyl_acceptor + L_Alanine		 (( (Vmax * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) buffer: 25 mM Tris-HCl, 100 mM NaCl, 100 µM DTT
PH: 8
Temperature: 37
uniprot:P9WQ71 [Pyridoxal phosphate]

L_Cysteine + Acceptor -> L_Alanine + S_sulfanyl_acceptor		 (( (Vmax * S) ) / (S + Km)) buffer: 200 mM Tris/HCl, 50 mM NaCl, 5 mM DTT
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7888 tRNA-uridine 2-thiolation (cytoplasmic)
BioCyc:HUMAN_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:PLASMO_PWY0-1021 alanine biosynthesis III
BioCyc:MTBH37RV_PWY-7250 [2Fe-2S] iron-sulfur cluster biosynthesis
BioCyc:MTBH37RV_PWY0-1021 L-alanine biosynthesis III
BioCyc:BSUB_PWY0-1021 L-alanine biosynthesis III
BioCyc:SYNEL_PWY0-1021 L-alanine biosynthesis III
BioCyc:HUMAN_PWY-7889 tRNA-uridine 2-thiolation (mammalian mitochondria)
BioCyc:META_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:META_PWY-7889 tRNA-uridine 2-thiolation (mammalian mitochondria)
BioCyc:YEAST_PWY-7250 iron-sulfur cluster biosynthesis
BioCyc:YEAST_PWY-7891 tRNA-uridine 2-thiolation (yeast mitochondria)
BioCyc:MTBH37RV_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:ECOL316407_PWY0-1061 superpathway of alanine biosynthesis
BioCyc:HUMAN_PWY-7250 [2Fe-2S] iron-sulfur cluster biosynthesis
BioCyc:META_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:META_PWY-7250 [2Fe-2S] iron-sulfur cluster biosynthesis
BioCyc:ECO_PWY0-1061 superpathway of L-alanine biosynthesis
BioCyc:ECO_PWY0-1021 L-alanine biosynthesis III
BioCyc:YEAST_PWY0-1021 L-alanine biosynthesis III
BioCyc:MTBCDC1551_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:SYNEL_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:SYNEL_PWY-7250 [2Fe-2S] iron-sulfur cluster biosynthesis
BioCyc:ECOL316407_PWY0-1021 alanine biosynthesis III
BioCyc:META_PWY0-1021 L-alanine biosynthesis III
BioCyc:META_PWY-7892 tRNA-uridine 2-thiolation (bacteria)
BioCyc:META_PWY-7888 tRNA-uridine 2-thiolation (cytoplasmic)
BioCyc:ECO_PWY-7892 tRNA-uridine 2-thiolation (bacteria)
BioCyc:ECO_PWY-6823 molybdenum cofactor biosynthesis
BioCyc:ECOL316407_PWY-6823 molybdenum cofactor biosynthesis
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000810 L-Alanine Metabolism
Reactome:R-MMU-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-RNO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9865881 Complex III assembly
Reactome:R-RNO-9865881 Complex III assembly
Reactome:R-DDI-9865881 Complex III assembly
Reactome:R-DME-9865881 Complex III assembly
Reactome:R-SCE-9865881 Complex III assembly
Reactome:R-SPO-9865881 Complex III assembly
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-947581 Molybdenum cofactor biosynthesis
Reactome:R-DME-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-611105 Respiratory electron transport
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-947581 Molybdenum cofactor biosynthesis
Reactome:R-PFA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-611105 Respiratory electron transport
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-947581 Molybdenum cofactor biosynthesis
Reactome:R-RNO-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-SCE-611105 Respiratory electron transport
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-611105 Respiratory electron transport
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-947581 Molybdenum cofactor biosynthesis
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-PFA-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-947581 Molybdenum cofactor biosynthesis
Reactome:R-SPO-611105 Respiratory electron transport
Reactome:R-DDI-611105 Respiratory electron transport
Reactome:R-DME-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-PFA-1430728 Metabolism
Reactome:R-PFA-947581 Molybdenum cofactor biosynthesis
Reactome:R-PFA-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1362409 Mitochondrial iron-sulfur cluster biogenesis
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-947581 Molybdenum cofactor biosynthesis
PathBank:SMP0121272 L-Alanine Metabolism
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0002415 Alanine Metabolism