- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (( (( (( (( (PFKgR * PFKkcat) ) * (( (PFK1 + PFK2) ) - abs((PFK1 - PFK2)))) ) * ATP) ) * (1 - (( (ADP * F16bP) ) / (( (PFKKeq * ATP) ) * F6P)))) ) * F6P) ) * (( (( (( (( (( (1 + (ADP / PFKKadp)) ) + (ATP / PFKKatp)) ) + (F16bP / PFKKf16)) ) + (( (( (PFKgR * ADP) ) * F16bP) ) / (PFKKadp * PFKKf16))) ) + (F6P / PFKKf6p)) ) + (( (( (PFKgR * ATP) ) * F6P) ) / (PFKKatp * PFKKf6p)))) ) / (( (( (2 * PFKKatp) ) * PFKKf6p) ) * (( (( (( (( (( (PFKL0 * (( (1 + (( (PFKCamp * AMP) ) / PFKKamp)) ) ^ 2)) ) * (( (1 + (( (PFKCatp * ATP) ) / PFKKatp)) ) ^ 2)) ) * (( (1 + (( (PFKCiatp * ATP) ) / PFKKiatp)) ) ^ 2)) ) * (( (( (1 + (( (F26bP * PFKCf26) ) / PFKKf26)) ) + (( (PFKCf16 * F16bP) ) / PFKKf16)) ) ^ 2)) ) / (( (( (( (1 + (AMP / PFKKamp)) ) ^ 2) ) * (( (1 + (ATP / PFKKiatp)) ) ^ 2)) ) * (( (( (1 + (F26bP / PFKKf26)) ) + (F16bP / PFKKf16)) ) ^ 2))) ) + (( (( (( (( (( (( (1 + (ADP / PFKKadp)) ) + (ATP / PFKKatp)) ) + (F16bP / PFKKf16)) ) + (( (( (PFKgR * ADP) ) * F16bP) ) / (PFKKadp * PFKKf16))) ) + (F6P / PFKKf6p)) ) + (( (( (PFKgR * ATP) ) * F6P) ) / (PFKKatp * PFKKf6p))) ) ^ 2)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (( (vol * Vmax10) ) * 0.0575) ) * HexP) ) / (Km10F6P + (0.0575 * HexP))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype isoenzyme PFKM]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM triethanolamine-HCl, 10 mM MgCl2, 100 mM KCl, 1 mM phosphoenolpyruvate, 0.18 mM NADH, 10 U/ml porcine muscle lactate dehydrogenase, 10 U/ml rabbit muscle pyruvate kinase
PH: 8
Temperature: 22 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype isoenzyme PFKM]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM triethanolamine-HCl, 10 mM MgCl2, 100 mM KCl, 1 mM phosphoenolpyruvate, 0.18 mM NADH, 10 U/ml porcine muscle lactate dehydrogenase, 10 U/ml rabbit muscle pyruvate kinase
PH: 8
Temperature: 22 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (( (( (Vpf * VPFvPFK) ) * atpPF) ) * f6pPF) ) / (( (( (KatpPFvPFK * Vpf) ) * Kf6pPFvPFK) ) * Vpf)) ) / (( (( (1 + (atpPF / (KatpPFvPFK * Vpf))) ) * (( (1 + (adpPF / (KadpPFvPFK * Vpf))) ) + (atpPF / (KatpPFvPFK * Vpf)))) ) * (( (1 + (f16bpPF / (Kf16bpPFvPFK * Vpf))) ) + (f6pPF / (Kf6pPFvPFK * Vpf))))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris/Mes, 10 mM MgCl2, 0.2 mM NADH, aldolase, triose-phosphate-isomerase, glycerol phosphate dehydrogenase
PH: 6
Temperature: 28 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris/Mes, 10 mM MgCl2, 0.2 mM NADH, aldolase, triose-phosphate-isomerase, glycerol phosphate dehydrogenase
PH: 6
Temperature: 28 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 60 mM Tris/HCl, 10 mM 2-mercaptoethanol, 1 mM magnesium acetate, 10 mM NH4HCO3, 0.2 mM NADH, 100 µg/ml aldolase, 7 µg/ml mixture of triosephosphate isomerase + alpha-glycerolphosphate dehydrogenase
PH: 7.2
Temperature: 30 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + Fructose_6_phosphate -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris/Mes, 10 mM MgCl2, 0.2 mM NADH, aldolase, triose-phosphate-isomerase, glycerol phosphate dehydrogenase
PH: 8.2
Temperature: 28 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.1 M Tris/Mes, 10 mM MgCl2, 0.2 mM NADH, aldolase, triose-phosphate-isomerase, glycerol phosphate dehydrogenase
PH: 8.2
Temperature: 28 |
- [6-phosphofructokinase(Enzyme) wildtype Pfk1p and Pfk2p, C-terminal GST-tagged]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 100 mM MES, 100 mM KCl, 1.5 U/ml aldolase, 50 U/ml triose phosphate isomerase, 4.3 U/ml glycerol-3-phosphate dehydrogenase, 1 mM AMP, 0.2 mM NADH, 5 mM Mg2+
PH: 6.5
Temperature: 30 |
uniprot:P08237 [6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes, 100 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 1.2 U/ml aldolase, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 25 |
uniprot:P90521 [6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes, 100 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 1.2 U/ml aldolase, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 25 |
uniprot:P08237 [6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes, 100 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 1.2 U/ml aldolase, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:P90521 [6-phosphofructokinase(Enzyme) mutant 4 N-terminal and 4 C-terminal subunits]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Hepes, 100 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 1.2 U/ml aldolase, 10 U/ml triosephosphate isomerase, 1 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM PIPES, 0.1 mM KCl , 8 mM MgCl2, 10 mM NH4Cl, 2.5 mM 2-Mercaptoethanol, 0.2 mM NADH, 8.25 units/ml pyruvate kinase, 21.0 units/ml lactic dehydrogenase
PH: 7.23
Temperature: 22 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM PIPES, 0.1 mM KCl , 8 mM MgCl2, 10 mM NH4Cl, 2.5 mM 2-Mercaptoethanol, 0.2 mM NADH, 8.25 units/ml pyruvate kinase, 21.0 units/ml lactic dehydrogenase
PH: 7.52
Temperature: 22 |
- [AMP]
ATP + beta_D_Fructose_6_phosphate -> ADP + beta_D_Fructose_1,6_bisphosphate |
(( (( (( (( (( (( (( (cytosol * Vmax_4) ) * gR_4) ) * F6P) ) / Kf6p_4) ) * ATP) ) / Katp_4) ) * (( (( (1 + (F6P / Kf6p_4)) ) + (ATP / Katp_4)) ) + (( (( (( (gR_4 * F6P) ) / Kf6p_4) ) * ATP) ) / Katp_4))) ) / (( (( (( (( (1 + (F6P / Kf6p_4)) ) + (ATP / Katp_4)) ) + (( (( (( (gR_4 * F6P) ) / Kf6p_4) ) * ATP) ) / Katp_4)) ) ^ 2) ) + (( (( (( (L0_4 * (( (( (1 + (( (Ciatp_4 * ATP) ) / Kiatp_4)) ) / (1 + (ATP / Kiatp_4))) ) ^ 2)) ) * (( (( (1 + (( (Camp_4 * AMP) ) / Kamp_4)) ) / (1 + (AMP / Kamp_4))) ) ^ 2)) ) * (( (( (( (1 + (( (Cf26_4 * F26bP) ) / Kf26_4)) ) + (( (Cf16_4 * F16bP) ) / Kf16_4)) ) / (( (1 + (F26bP / Kf26_4)) ) + (F16bP / Kf16_4))) ) ^ 2)) ) * (( (1 + (( (Catp_4 * ATP) ) / Katp_4)) ) ^ 2)))) |
|
buffer: *
PH: 7
Temperature: 36 |
- [AMP]
ATP + beta_D_Fructose_6_phosphate -> beta_D_Fructose_1,6_bisphosphate + ADP |
(( (( (( (( (( (( (( (cytosol * VmPFK) ) * gR) ) * F6P) ) / KmPFKF6P) ) * ATP) ) / KmPFKATP) ) * (( (( (1 + (F6 / KmF6P)) ) + (AT / KmATP)) ) + (( (( (( (g * F6) ) / KmF6P) ) * AT) ) / KmATP))) ) / (( (( (( (( (1 + (F6 / KmF6P)) ) + (AT / KmATP)) ) + (( (( (( (g * F6) ) / KmF6P) ) * AT) ) / KmATP)) ) ^ 2) ) + (( (( (( (L * (( (( (1 + (( (CiATP * AT) ) / KiATP)) ) / (1 + (AT / KiATP))) ) ^ 2)) ) * (( (( (1 + (( (CAMP * AM) ) / KAMP)) ) / (1 + (AM / KAMP))) ) ^ 2)) ) * (( (( (( (1 + (( (CF26BP * F26) ) / KF26BP)) ) + (( (CF16BP * F16) ) / KF16BP)) ) / (( (1 + (F26 / KF26BP)) ) + (F16 / KF16BP))) ) ^ 2)) ) * (( (1 + (( (CATP * AT) ) / KmATP)) ) ^ 2)))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:O08333 [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U of fructose biphosphate aldolase, 5 U of triose phosphate isomerase, 0.85 U of a-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:O08333 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U of fructose biphosphate aldolase, 5 U of triose phosphate isomerase, 0.85 U of a-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
uniprot:O08333 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U of fructose biphosphate aldolase, 5 U of triose phosphate isomerase, 0.85 U of a-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 30 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:O15648 [AMP]
ATP + Fructose_6_phosphate -> ADP + Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M triethanolamine hydrochloride, 1 mM EDTA, 0.6 mM NADH, 2.5 mM MgCl2, 1 mM NaHCO3, 0.9 U/ml aldolase, 5 U/ml TIM, 1.7 U/ml GPDH
PH: 7.6
Temperature: 25 |
uniprot:O15648 [AMP]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M triethanolamine hydrochloride, 1 mM EDTA, 0.42 mM NADH, 2.2 mM PEP, 2.5 mM MgCl2, 1 mM NaHCO3, 1.1 U/ml PyK/LDH (1/1)
PH: 7.6
Temperature: 25 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(Vo + (( (( (V - Vo) ) * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n)))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(Vo + (( (( (V - Vo) ) * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n)))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(Vo + (( (( (V - Vo) ) * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n)))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(Vo + (( (( (V - Vo) ) * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n)))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(Vo + (( (V - Vo) ) * (( (( (S / S_half) ) ^ n) ) / (1 + (( (S / S_half) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(Vo + (( (V - Vo) ) * (( (( (S / S_half) ) ^ n) ) / (1 + (( (S / S_half) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q4E657 [Mg2+]
ATP + beta_D_Fructose_6_phosphate -> ADP + beta_D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine/HCl, 2.5 mM MgCl2, 0.42 mM NADH, 0.4 U/ml aldolase, 0.8 U/ml glycerol-3-phosphate dehydrogenase, 20 U/ml triosephosphate isomerase
PH: 8
Temperature: 25 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / S_half) ) ^ n)) ) / (1 + (( (S / S_half) ) ^ n))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S / (Ks + S))) ) * (( (1 + (( (S / Kr) ) ^ n)) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM imidazol/HCl, 25 mM KH2PO4/K2HPO4, 10 mM MgCl2, 100 KCl, 0.25 mM NADH, 0.5 mM phosphoenolpyruvate, 2 U/ml lactate dehydrogenase, 2 U/ml pyruvate kinase, 0.5 µg/ml phosphofructokinase, 0.2 U/ml fructose-1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (( (S / S) ) + Ks)) ) * (1 / (1 + (Lo * (( (( (1 + (S / Kt)) ) / (1 + (S / Kr))) ) ^ 8))))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (( (S / S) ) + Ks)) ) * (1 / (1 + (Lo * (( (( (1 + (S / Kt)) ) / (1 + (S / Kr))) ) ^ 8))))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q9BIC6 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM TEA/HCl, 2.5 mM MgCl2, 0.42 mM NADH, 0.4 U/ml aldolase, 0.8 U/ml glycerol-3-phosphate dehydrogenase, 20 U/ml triosephosphate isomerase
PH: 8
Temperature: 25 |
uniprot:Q03215 [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / Ks) ) + S)) ) * (( (( (1 + (S / Kr)) ) ^ n) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(Vo + (( (V - Vo) ) * (( (( (S / S_half) ) ^ n) ) / (1 + (( (S / S_half) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / S) ) + Ks)) ) * (1 / (1 + (Lo * (( (( (1 + (S / Kt)) ) / (1 + (S / Kr))) ) ^ 8))))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q8NJU8 [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(Vo + (( (V - Vo) ) * (( (( (S / S_half) ) ^ n) ) / (1 + (( (S / S_half) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 100 mM KCl, 10 mM MgCl2, 20 mM potassium phosphate, 0.2 mM NADH, 0.6 mM PEP, 8.5 U/ml pyruvate kinase, 7 U/ml lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (( (S / Ks) ) + S)) ) * (( (( (1 + (S / Kr)) ) ^ n) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (( (S / Ks) ) + S)) ) * (( (( (1 + (S / Kr)) ) ^ n) ) / (( (( (1 + (S / Kr)) ) ^ n) ) + (Lo * (( (1 + (S / Kt)) ) ^ n))))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:Q03215 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole/HCl, 20 mM potassium phosphate, 10 mM MgCl2, 100 mM KCl, 0.2 mM NADH, 0.6 mM Phosphoenolpyruvate, 8.5 U/ml Pyruvate kinase, 7 U/ml Lactate dehydrogenase, 1 U/ml Fructose 1,6-bisphosphatase
PH: 6.6
Temperature: 25 |
uniprot:P47857 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (A ^ n)) ) / (( (S_h ^ n) ) + (A ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 10 mM NH2PO4, 10 mM MgCl2
PH: 7.4
Temperature: 37 |
uniprot:P30835 [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S - (P / Keq))) ) / (Ks * (( (1 + (S / Ks)) ) + (P / Kp)))) |
|
buffer: 50 mM Hepes / 10 mM sodium phosphate, 100 mM KCl, 10 mM MgCl2, 1 mg/ml trypsin inhibitor, 0.28 mM NADH, 2.5 mM phosphocreatine, 5 U/ml creatine kinase, 1 U/ml fructose-bisphosphate aldolase, 5 U/ml triose-phosphate isomerase, 3 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.4
Temperature: 35 |
uniprot:P47858 [6-phosphofructokinase(Enzyme) wildtype]
ATP + beta_D_Fructose_6_phosphate -> ADP + beta_D_Fructose_1,6_bisphosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38 |
uniprot:P47858 [6-phosphofructokinase(Enzyme) wildtype]
beta_D_Fructose_6_phosphate + ATP -> ADP + beta_D_Fructose_1,6_bisphosphate |
(( (V * A) ) / (Km_A + A)) |
|
buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (V * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 50 mM triethanolamine chloride, 3 mM MgCl2, 5 mM mercaptoethanol, 0.16 mM NADH, 0.64 mM AMP, 3.3 µg/ml aldolase, 0.2 µg/ml triose phosphate isomerase, 1.48 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P30835 [6-phosphofructokinase(Enzyme) wildtype]
ADP + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + ATP |
(( (( (V * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Ka * B)) ) + (Kb * A)) ) + (A * B))) |
|
buffer: 50 mM triethanolamine chloride, 3 mM MgCl2, 5 mM mercaptoethanol, 0.35 mM NADP+, 5.0 mM glucose, 0.64 mM AMP, 8.8 µg/ml hexokinase, 4.4 µg/ml phosphoglucose isomerase, 4.4 µg/ml glucose 6-phosphate dehydrogenase, 4.4 µg/ml 6-phosphogluconate dehydrogenase
PH: 8
Temperature: 30 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 6
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 7.6
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 5.5
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 7.6
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 50 mM KCl, 1.0 mM phosphoenolpyruvate, 0.5 mM NADH, 30 µg/ml pyruvate kinase, 30 µg/ml lactate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q5FKG6 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ nH)) ) / (( (Sh ^ nH) ) + (S ^ nH))) |
|
buffer: 50 mM triethanolamine HCl, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 8.5
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 5.5
Temperature: 25 |
uniprot:Q88VY1 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * S) ) / (Km + S)) |
|
buffer: 0.1 M phosphate, 4.0 mM MgCl2, 0.5 mM NADH, 60 µg/ml fructose-1,6-bisphosphate aldolase, 5 µg/ml triosephosphate isomerase, 40 µg/ml glycerol 1-phosphate dehydrogenase
PH: 7.6
Temperature: 25 |
uniprot:Q4E657 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 2 U/ml Aldolase, 2 U/ml Triosephosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase, 0.2 mM NADH, 90 mM KCl, 4 mM Mercaptoethanol
PH: 7.4
Temperature: 25 |
uniprot:Q4E657 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 2 U/ml Aldolase, 2 U/ml Triosephosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase, 0.2 mM NADH, 90 mM KCl, 4 mM Mercaptoethanol
PH: 7.4
Temperature: 25 |
uniprot:Q4E657 [6-phosphofructokinase(Enzyme) wildtype]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 2 U/ml Aldolase, 2 U/ml Triosephosphate isomerase, 2 U/ml Glycerol-3-phosphate dehydrogenase, 0.2 mM NADH, 90 mM KCl, 4 mM Mercaptoethanol
PH: 7.4
Temperature: 25 |
uniprot:Q4E657 [Mn2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
uniprot:Q4E657 [Mn2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
uniprot:Q4E657 [Mn2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * M) ) / (KmM + M)) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
uniprot:Q4E657 [Mn2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (KmB + B)) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
uniprot:Q4E657 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (Vmax * A) ) * B) ) / (( (( (( (KmA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
uniprot:Q4E657 [Mn2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (KmA + A)) |
|
buffer: 50 mM HEPES, 90 mM KCl, 5 mM mercaptoethanol, 0.2 mM NADH, 50 U/ml aldolase, 50 U/ml triosephosphate isomerase, 50 U/ml glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 23 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole-HCl, 10 mM MgCl2, 2 mM Mercaptoethanol, 0.5 mM Phosphoenolpyruvate, 0.15 mM NADH, Pyruvate kinase, Lactate dehydrogenase
PH: 6.6
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * (S ^ n)) ) / (( (S_h ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole-HCl, 10 mM MgCl2, 2 mM Mercaptoethanol, 0.5 mM Phosphoenolpyruvate, 0.15 mM NADH, Pyruvate kinase, Lactate dehydrogenase
PH: 6.6
Temperature: 25 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 37 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 37 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 50 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 50 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 25 |
uniprot:Q9L9E3 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (V * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 5 mM MgCl2, 0.3 mM NADH, 10 µg/ml Aldolase, 5 µg/ml Glycerol 1-phosphate dehydrogenase, 2 µg/ml Triosephosphate isomerase
PH: 7
Temperature: 25 |
uniprot:Q01813 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
ITP + D_Fructose_6_phosphate -> IDP + D_Fructose_1,6_bisphosphate |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ITP -> IDP + D_Fructose_1,6_bisphosphate |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(V / (( (( (1 + (KmS / (S ^ n))) ) + (KmA / A)) ) + (KmAS / (( (S ^ n) ) * A)))) |
|
buffer: 0.2 M Tris HCl, 6 mM KCl, 10 mg/ml Fructose diphosphate aldolase, 2 mg/ml Triosephosphate isomerase, 2 mg/ml Glycerophosphate dehydrogenase, 0.2 mM NADH
PH: 8
Temperature: 25 |
uniprot:P30835 [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM KCl, 50 mM Mops, 0.5 mM EGTA
PH: 7
Temperature: 37 |
uniprot:P30835 [NH4+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM KCl, 50 mM Mops, 0.5 mM EGTA
PH: 7
Temperature: 37 |
uniprot:P30835 [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM KCl, 50 mM Mops, 0.5 mM EGTA
PH: 7
Temperature: 37 |
uniprot:P30835 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Mops, 5 mM MgCl2, 1 mM EDTA, 0.15 mM NADH, 0.36 U/ml Aldolase, 9 U/ml Triosephosphate isomerase, 3.1 U/ml alpha-Glyceraldehyde 3-phosphate dehydrogenase
PH: 7
Temperature: 37 |
uniprot:P30835 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Mops, 5 mM MgCl2, 1 mM EDTA, 0.15 mM NADH, 0.36 U/ml Aldolase, 9 U/ml Triosephosphate isomerase, 3.1 U/ml alpha-Glyceraldehyde 3-phosphate dehydrogenase
PH: 7
Temperature: 37 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM imidazole-HCl, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 IU aldolase, 1 IU triosephosphate isomerase, 2 IU alpha-glycerolphosphate dehydrogenase
PH: 7
Temperature: 6 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM imidazole-HCl, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 IU aldolase, 1 IU triosephosphate isomerase, 2 IU alpha-glycerolphosphate dehydrogenase
PH: 7
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM imidazole-HCl, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 IU aldolase, 1 IU triosephosphate isomerase, 2 IU alpha-glycerolphosphate dehydrogenase
PH: 7
Temperature: 6 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM imidazole-HCl, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 IU aldolase, 1 IU triosephosphate isomerase, 2 IU alpha-glycerolphosphate dehydrogenase
PH: 7
Temperature: 20 |
uniprot:P0A796 [6-phosphofructokinase(Enzyme) wildtype His-tagged]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (Vmax * (A ^ n)) ) * B) ) / (( (( (( (( (KmA ^ n) ) * KmB) ) + (KmB * (A ^ n))) ) + (( (KmA ^ n) ) * B)) ) + (( (A ^ n) ) * B))) |
|
buffer: 100 mM MOPS, 2 mM DTT,0.1 mM NADP, 5 mM MgCl2, 1.8 U/ml ALD, 31 U/ml G3PDH, 90 U/ml TIM
PH: 7.2
Temperature: 37 |
uniprot:Q8VU09 [Diphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (K50 + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q8VU09 [Diphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (K50 + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q8VU09 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (K50 + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
- [6-phosphofructokinase(Enzyme) mutant Y41L]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41F]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41W]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41A]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41A]
ITP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41F]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41L]
ITP + D_Fructose_6_phosphate -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41W]
D_Fructose_6_phosphate + ITP -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ITP + D_Fructose_6_phosphate -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77A]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77L]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82A]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77D]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77E]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82E]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76A]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76Y]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76W]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82E]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111A]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82A]
UTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + UDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77D]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77L]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77E]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [Mg2+]
D_Fructose_1,6_bisphosphate + ADP -> ATP + D_Fructose_6_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 85 |
- [6-phosphofructokinase(Enzyme) mutant F76W]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76A]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76Y]
ITP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76W]
CTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76Y]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76A]
CTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76Y]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76A]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76W]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77A]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111A]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77L]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82A]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82E]
CTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77A]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77E]
CTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77D]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
CTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
D_Fructose_6_phosphate + ITP -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
D_Fructose_6_phosphate + UTP -> D_Fructose_1,6_bisphosphate + UDP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
UTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + UDP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK1]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 8
Temperature: 25 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Pipes, 100 mM KCl, 5 mM magnesium acetate,0.25 μM aldolase, 0.41 μM triose-phosphate isomerase, 0.9 μM glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 20 mM Imidazole-HCl, 5 mM MgCl2, 20 mM KCl, 0.15 mM NADH, 0.5 U aldolase, 0.5 U triosephosphate isomerase, 1 U glycerol 3-phosphate dehydrogenase
PH: 7.2
Temperature: 22 |
uniprot:A1XM06 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES/NaOH, 0.5 mM MgCl2, 0.15 mM NADH, 2 mM dithiothreitol, 50 U/ml triosephosphate isomerase, 5 U/ml glycerol 3-phosphate dehydrogenase, 5 U/ml aldolase
PH: 7.8
Temperature: 30 |
uniprot:A1XM06 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES/NaOH, 0.5 mM MgCl2, 0.15 mM NADH, 2 mM dithiothreitol, 50 U/ml triosephosphate isomerase, 5 U/ml glycerol 3-phosphate dehydrogenase, 5 U/ml aldolase, 15 U/ml creatine kinase, 1 mM creatine phosphate
PH: 7.8
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41W]
D_Fructose_6_phosphate + CTP -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41F]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41A]
D_Fructose_6_phosphate + CTP -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41L]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41A]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41F]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41W]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111A]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111A]
ITP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77D]
ITP + D_Fructose_6_phosphate -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77A]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77L]
D_Fructose_6_phosphate + ITP -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82E]
ITP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82A]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77E]
D_Fructose_6_phosphate + ITP -> IDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Ka + A)) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Ka + A)) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Ka + A)) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Ka + A)) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes-KOH, 50 mM KCl, 2 mM DTT, 0.1 mM NADH, 1.6 U/ml Aldolase, 6.3 U/ml Triose-phosphate isomerase, 3.0 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 20 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 85 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 85 |
- [Mg2+]
D_Fructose_1,6_bisphosphate + ADP -> D_Fructose_6_phosphate + ATP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 85 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 50 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl
PH: 6
Temperature: 50 |
uniprot:Q8VU09 [Diphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (K50 + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q8VU09 [Diphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K50 + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q8VU09 [Diphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
uniprot:Q8VU09 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 1 mM KCl, 3 mM NH4Cl, 5 mM dithiothreitol, 0.15 mM NADH, 0.9 U FBP aldolase, 5 U triose phosphate isomerase, 0.85 U alpha-glycerol-3-phosphate dehydrogenase
PH: 7.5
Temperature: 37 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76W]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76A]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant F76Y]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41A]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41W]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41L]
D_Fructose_6_phosphate + UTP -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41F]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant Y41L]
D_Fructose_6_phosphate + CTP -> D_Fructose_1,6_bisphosphate + CDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111A]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77L]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77E]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82A]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77D]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R77A]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R82E]
D_Fructose_6_phosphate + GTP -> D_Fructose_1,6_bisphosphate + GDP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 150 mM Tes/KOH, 1 mM EDTA, 2.5 mM MgCl2, 0.2 mM NADH, 1 mM GDP, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triosephosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> ATP + D_Fructose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 25 mM MgCl2
PH: 7
Temperature: 50 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> ATP + D_Fructose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 25 mM MgCl2
PH: 7
Temperature: 50 |
uniprot:O42938 [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(Vmax + (( (V - Vmax) ) * (( (( (M / M_half) ) ^ nH) ) / (1 + (( (M / M_half) ) ^ nH))))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(Vmax * (( (( (S / S_half) ) ^ nH) ) / (1 + (( (S / S_half) ) ^ nH)))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(Vmax * (( (( (S / S_half) ) ^ nH) ) / (1 + (( (S / S_half) ) ^ nH)))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S / (S + Ks))) ) * (( (1 / (1 + (( (m0 * (( (1 + (S / K_T)) ) / (1 + (M / K_R)))) ) ^ 8))) ) + (alpha * (( (( (m0 * (( (1 + (S / K_T)) ) / (1 + (M / K_R)))) ) ^ 8) ) / (1 + (( (m0 * (( (1 + (S / K_T)) ) / (1 + (M / K_R)))) ) ^ 8)))))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(Vmax * (( (( (S / S_half) ) ^ nH) ) / (1 + (( (S / S_half) ) ^ nH)))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(Vmax + (( (V - Vmax) ) * (( (( (M / M_half) ) ^ nH) ) / (1 + (( (M / M_half) ) ^ nH))))) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:O42938 [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM imidazole/HCl, 5 mM MgCl2, 0.2 mM NADH, 1 U/ml aldolase, 1.5 U/ml glycerol phosphate dehydrogenase, 5 U/ml triosephosphate isomerase
PH: 7.2
Temperature: 25 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 75 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 50 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 75 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 50 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 80 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
ADP + D_Fructose_1,6_bisphosphate -> ATP + D_Fructose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 25 mM MgCl2
PH: 7
Temperature: 75 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 75 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 10 mM MgCl2
PH: 7
Temperature: 75 |
uniprot:Q9WY52 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> ATP + D_Fructose_6_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM triethanolamine, 25 mM MgCl2
PH: 7
Temperature: 75 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
D_Fructose_6_phosphate + ITP -> D_Fructose_1,6_bisphosphate + IDP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype PFK2]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES-KOH, 5 mM Mg-acetate, 0.15 mM NADH, 1 U/ml aldolase, 10 U/ml triose-phosphate isomerase, 1 U/ml glycerol 3-phosphate dehydrogenase
PH: 7.3
Temperature: 25 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM EPPS, 10 mM MgCl2, 10 mM NH4Cl, 0.1 mM EDTA, 2 mM DTT, 250 µg aldolase, 50 µg glycerol-3-phosphate dehydrogenase, 25 mg triose phosphate isomerase
PH: 8
Temperature: 25 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM EPPS, 10 mM MgCl2, 10 mM NH4Cl, 0.1 mM EDTA, 2 mM DTT, 250 µg aldolase, 50 µg glycerol-3-phosphate dehydrogenase, 25 mg triose phosphate isomerase
PH: 6
Temperature: 25 |
uniprot:P80019 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM EPPS, 10 mM MgCl2, 10 mM NH4Cl, 0.1 mM EDTA, 2 mM DTT, 250 µg aldolase, 50 µg glycerol-3-phosphate dehydrogenase, 25 mg triose phosphate isomerase
PH: 8
Temperature: 25 |
- [GDP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.50 mM Mg2+
PH: 8.5
Temperature: 30 |
- [GDP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.10 mM Mg2+
PH: 8.5
Temperature: 30 |
- [GDP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.05 mM Mg2+
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R72H]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.0094 - 4.94 mM Mg2+
PH: 8.5
Temperature: 30 |
uniprot:P16861 [6-phosphofructokinase(Enzyme) wildtype 6-phosphofructokinase subunit alpha]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * Et) ) * S) ) / (Ks + S)) |
|
buffer: 100.0 mM 2-(N-morpholino)ethanesulfonic acid, 5.0 mM magnesium dichloride, 0.2 mM NADH, 1.0 mM AMP, 100.0 mM potassium chloride
PH: 6.5
Temperature: 30 |
uniprot:P16861 [6-phosphofructokinase(Enzyme) wildtype 6-phosphofructokinase subunit alpha]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * Et) ) * S) ) / (Ks + S)) |
|
buffer: 100.0 mM 2-(N-morpholino)ethanesulfonic acid, 5.0 mM magnesium dichloride, 0.2 mM NADH, 1.0 mM AMP, 100.0 mM potassium chloride
PH: 6.5
Temperature: 30 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Mes, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 8
Temperature: 20 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R111E]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
Adenosine_5_O_(3_thiotriphosphate) + D_Fructose_6_phosphate -> D_Fructose_6_phosphate_gamma_S + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
uniprot:P00512 [beta,gamma-Methylene ATP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [beta,gamma-Methylene ATP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [Adenylyl imidodiphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [Adenylyl imidodiphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + (B * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [Arabinose 5-phosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (K + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [Arabinose 5-phosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> ATP + D_Fructose_6_phosphate |
(( (( (E * kcat) ) * B) ) / (( (K * (1 + (I / Kis))) ) + (B * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADP+, 20 µg/ml hexokinase, 3 mM glucose, 10 µg/ml glucose-3-phosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (K + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 10 µg/ml pyruvate kinase, 10 µg/ml lactate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> D_Fructose_6_phosphate + ATP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADP+, 20 µg/ml hexokinase, 3 mM glucose, 10 µg/ml glucose-3-phosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-Cl, 4 mM Magnesium sulfate, 50 mM Potassium chloride, 1 mM Dithiothreitol, 0.2 mM EDTA, 0.2 mM Phosphoenolpyruvate, 0.16 mM NADH
PH: 7.8
Temperature: 28 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype]
2,5_Anhydro_D_mannitol_1_phosphate + ATP -> 2,5_Anhydro_D_mannitol_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-Cl, 4 mM Magnesium sulfate, 50 mM Potassium chloride, 1 mM Dithiothreitol, 0.2 mM EDTA, 0.2 mM Phosphoenolpyruvate, 0.16 mM NADH
PH: 7.8
Temperature: 28 |
uniprot:P47858 [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 0.2 mM EDTA, 5 mM MgCl2, 1 mM NH4Cl, 0.16 mM NADH, 2.5 mM DTT, 4.5 mM ATP, 0.25 mM citrate, 0.1 mM AMP, 5 mM Pi
PH: 7.2
Temperature: 25 |
- [Mg2+]
ATP + Sedoheptulose_7_phosphate -> Sedoheptulose_1,7_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
- [Mg2+]
Sedoheptulose_7_phosphate + ATP -> Sedoheptulose_1,7_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 5 mM Mg2+
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant I126A]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 5 mM Mg2+
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R72H]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 5 mM Mg2+
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.011 - 5.1 mM Mg2+
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant I126A]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 0.1 M MES, 0.051 M N-ethylmorpholine, 0.051 M diethanolamine, 1 mM GDP, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 54 µg/ml aldolase, 10 µg/ml glycerol-3-phosphate dehydrogenase, 2.24 µg/ml triosephosphate isomerase, 4.0055 - 4.55 mM Mg2+
PH: 8.5
Temperature: 30 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
Adenosine + ATP -> AMP + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose -> ADP + D_Fructose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
D_Ribose_5_phosphate + ATP -> ADP + D_Ribose_1,5_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
ATP + Glucose_6_phosphate -> ADP + Glucose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
Ribose + ATP -> ADP + Ribose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Mes, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 20 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Mes, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Mes, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 6.5
Temperature: 20 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_1,6_bisphosphate + ADP -> D_Fructose_6_phosphate + ATP |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kia * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADP+, 10 µg/ml phosphoglucoisomerase, 10 µg/ml glucose-3-phosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
ADP + D_Fructose_1,6_bisphosphate -> ATP + D_Fructose_6_phosphate |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADP+, 10 µg/ml phosphoglucoisomerase, 10 µg/ml glucose-3-phosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 10 µg/ml pyruvate kinase, 200 µM phosphoenolpyruvate, 10 µg/ml lactate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
ADP + D_Fructose_1,6_bisphosphate -> D_Fructose_6_phosphate + ATP |
(( (( (E * kcat) ) * B) ) / (( (K * (1 + (I / Kis))) ) + B)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADP+, 10 µg/ml phosphoglucoisomerase, 10 µg/ml glucose-3-phosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * B) ) / (B + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + UTP -> D_Fructose_1,6_bisphosphate + UDP |
(( (( (E * kcat) ) * B) ) / (B + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * B) ) / (B + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + GTP -> GDP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * B) ) / (B + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00511 [Ammonium sulfate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
- [Ammonium sulfate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Ammonium sulfate]
ATP + Sedoheptulose_7_phosphate -> Sedoheptulose_1,7_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
- [Ammonium sulfate]
Sedoheptulose_7_phosphate + ATP -> Sedoheptulose_1,7_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Mg2+]
ATP + Sedoheptulose_7_phosphate -> Sedoheptulose_1,7_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Mg2+]
ATP + Sedoheptulose_7_phosphate -> ADP + Sedoheptulose_1,7_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Mg2+]
Sedoheptulose_7_phosphate + ATP -> ADP + Sedoheptulose_1,7_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, NADH, Fructose 1,6 bisphosphate aldolase, triosephosphate isomerase, glycerol-3-phosphate dehydrogenase, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
uniprot:Q9YG89 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM sodium phosphate, 10 µg Enzyme, 8 mM MgCl2
PH: 6.7
Temperature: 85 |
- [Glycerate 3-phosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [2-Oxoglutarate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Imidazole, 0.1 mM NADH, 1 IU aldolase, 10 IU triosephosphate isomerase, 1 IU glycerol-3-phosphate dehydrogenase
PH: 7.2
Temperature: 20 |
- [6-phosphofructokinase(Enzyme) mutant M169L]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_1_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + Adenosine_5_O_(3_thiotriphosphate) -> D_Fructose_6_phosphate_gamma_S + ADP |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + 1_Carboxymethyl_ATP -> D_Fructose_1,6_bisphosphate + 1_Carboxymethyl_ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tes/KOH, 1 mM EDTA, 0.2 mM NADH, 1 mM GDP, 4 mM MgCl2, 1 mM dithiothreitol, 0.6 U/ml aldolase, 0.3 U/ml triose-phosphate isomerase, 0.3 U/ml glycerophosphate dehydrogenase
PH: 7.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (Kib * Kb) ) + (Kb * A)) ) + (Ka * B)) ) + (A * B))) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (( (K * (1 + (I / Kis))) ) + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (A + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
UTP + D_Fructose_6_phosphate -> UDP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (A + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
CTP + D_Fructose_6_phosphate -> CDP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (A + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00512 [6-phosphofructokinase(Enzyme) wildtype]
GTP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + GDP |
(( (( (E * kcat) ) * A) ) / (A + Km)) |
|
buffer: 100 mM Tris-HCl, 10 mM MgCl2, 5 mM NH4Cl, 0.2 mM NADH, 20 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml alpha-glycerophosphate dehydrogenase
PH: 8.2
Temperature: 30 |
uniprot:P00511 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
uniprot:P00511 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris-HCl, 0.15 mM NADH, 2.5 mM 2-mercaptoethanol
PH: 7.5
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl (pH7.2), 0.15 mM NADH + H+, auxiliary enzymes (200 munits rabbit muscle aldolase, 200 munits alpha-glycerophosphate dehydrogenase and 1.13 units triose phosphate isomerase)
PH: 7.2
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl (pH7.2), 0.15 mM NADH + H+, auxiliary enzymes (200 munits rabbit muscle aldolase, 200 munits alpha-glycerophosphate dehydrogenase and 1.13 units triose phosphate isomerase)
PH: 7.2
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl (pH7.2), 0.15 mM NADH + H+, auxiliary enzymes (200 munits rabbit muscle aldolase, 200 munits alpha-glycerophosphate dehydrogenase and 1.13 units triose phosphate isomerase)
PH: 7.2
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * A) ) / (Km + A)) |
|
buffer: 33 mM Tris-HCl (pH7.2), 0.15 mM NADH + H+, auxiliary enzymes (200 munits rabbit muscle aldolase, 200 munits alpha-glycerophosphate dehydrogenase and 1.13 units triose phosphate isomerase)
PH: 7.2
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 7.5 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 25 |
uniprot:P47860 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 0.05 M Tris-HCl, 3 mM MgCl2, 5 mM 2-mercaptoethanol, 0.34 mM NADH, 3.3 µg/ml aldolase, 1.48 µg/ml alpha-glycerolphosphate dehydrogenase, 0.2 µg/ml triosephosphate isomerase
PH: 7.6
Temperature: 30 |
uniprot:P47860 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Tris-HCl, 3 mM MgCl2, 5 mM 2-mercaptoethanol, 0.34 mM NADH, 3.3 µg/ml aldolase, 1.48 µg/ml alpha-glycerolphosphate dehydrogenase, 0.2 µg/ml triosephosphate isomerase
PH: 8.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant D127S:R252Q]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant D127S]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R252Q]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant D127S]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant R252Q]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 8
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer:
PH: 7
Temperature: 25 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [D-Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine/Cl, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 1 mM EDTA, 50 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 8
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Triethanolamine/Cl, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 1 mM EDTA, 50 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 8
Temperature: 30 |
uniprot:P47860 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 0.05 M Tris-HCl, 3 mM MgCl2, 5 mM 2-mercaptoethanol, 0.34 mM NADH, 3.3 µg/ml aldolase, 1.48 µg/ml alpha-glycerolphosphate dehydrogenase, 0.2 µg/ml triosephosphate isomerase
PH: 8.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Tris/Cl, 100 mM KCl, 0.15 mM NADH, 50 µg/ml aldolase, 1 µg/ml triose phosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) |
|
buffer: 0.1 M 2-[N-morpholino]ethane sulfonic acid, 0.051 M N-ethylmorpholine, 0.051 M Diethanolamine, 0.2 mM NADH, 1 mM dithiothreitol, 1 mM EDTA, 1 mM GDP, 54 µg/ml aldolase, 10 µg/ml glyerol-3-phosphate dehydrogenase, 2.2 µg/ml triosephosphate isomerase, 5 mM MgCl2
PH: 8.5
Temperature: 30 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Potassium phosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
uniprot:P47857 [Cu2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes, 100 mM KCl, 10 mM NaH2PO4, 10 mM MgCl2
PH: 7.4
Temperature: 37 |
uniprot:P47857 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Hepes, 100 mM KCl, 10 mM NaH2PO4, 10 mM MgCl2
PH: 7.4
Temperature: 37 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [AMP]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 7.5 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 7.5 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 25 |
- [AMP]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 25 |
- [D-Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 5 mM MgCl2, 0.13 mM NADH, 4 mM 2-mercaptoethanol, 0.2 mM EDTA, 25 mM KCl, 0.25 U/ml aldolase, 1 U/ml alpha-glycerophosphate dehydrogenase, 4.5 U/ml triosephosphate isomerase
PH: 7.4
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S2 ^ n)) ) / (( (S2_half ^ n) ) + (S2 ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Fructose 2,6-bisphosphate]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n))) |
|
buffer: 50 mM Imidazole, 50 mM KCl, 5 mM MgCl2, 0.15 mM NADH, 0.5 U Aldolase, 1 U Triose phosphate isomerase, 2 U alpha-Glycerol phosphate dehydrogenase
PH: 7
Temperature: 23 |
- [Citrate]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
Fructose_6_phosphate + ATP -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P17858 [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P17858 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P17858 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P08237 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P17858 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P08237 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P08237 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:P08237 [Mg2+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:Q01813 [Mg2+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:Q01813 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
uniprot:Q01813 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM Glycyl-glycine, 1 mM (NH4)2SO4, 0.5 mg/ml Bovine albumin, 5 mM Dithiothreitol, 100 mM KCl, 0.2 mM NADH, 0.5 IU/ml Aldolase, 10 IU/ml Triose phosphate isomerase, 0.5 IU/ml Glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 30 |
- [AMP]
Fructose_6_phosphate + ATP -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [AMP]
ATP + Fructose_6_phosphate -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [AMP]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
ATP + Fructose_6_phosphate -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [Citrate]
Fructose_6_phosphate + ATP -> ADP + Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 50 mM HEPES, 100 mM KCl, 3 mM MgCI2, 20 mM (NH4)2SO4, 2 mM DTT, 0.1 mM Na4EDTA, 0.2 mM NADH, 0.5 U/ml fructose 1,6-bisphosphate aldolase, 1 U/ml triosephosphate isomerase, 0.5 U/ml glycerol-3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype from infected cells]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 100 mM (NH4)2SO4
PH: 7.4
Temperature: 37 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 100 mM (NH4)2SO4
PH: 7.4
Temperature: 37 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 67 mM Glycylglycine, 0.15 mM NADH, 0.05 mg/ml Bovine serum albumin, 0.12 mM 1,4-Dithiothreitol, 0.6 IU aldolase, 0.6 IU glycerol-3-phosphate dehydrogenase, 1.0 IU triosephosphate isomerase
PH: 9
Temperature: 37 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM McCl2, 100 mM KCl, 0.15 mM NADH, 0.1 mM AMP, 1 mM NH4Cl, 5 mM KH2PO4, 0.5 U/ml aldolase, 0.5 U/ml alpha-glycerolphosphate dehydrogenase, 5 U/ml triose phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM McCl2, 100 mM KCl, 0.15 mM NADH, 0.1 mM AMP, 1 mM NH4Cl, 5 mM KH2PO4, 0.5 U/ml aldolase, 0.5 U/ml alpha-glycerolphosphate dehydrogenase, 5 U/ml triose phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM McCl2, 100 mM KCl, 0.15 mM NADH, 0.1 mM AMP, 1 mM NH4Cl, 5 mM KH2PO4, 0.5 U/ml aldolase, 0.5 U/ml alpha-glycerolphosphate dehydrogenase, 5 U/ml triose phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM McCl2, 100 mM KCl, 0.15 mM NADH, 0.1 mM AMP, 1 mM NH4Cl, 5 mM KH2PO4, 0.5 U/ml aldolase, 0.5 U/ml alpha-glycerolphosphate dehydrogenase, 5 U/ml triose phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7
Temperature: 25 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM HEPES, 5 mM McCl2, 100 mM KCl, 0.15 mM NADH, 0.1 mM AMP, 1 mM NH4Cl, 5 mM KH2PO4, 0.5 U/ml aldolase, 0.5 U/ml alpha-glycerolphosphate dehydrogenase, 5 U/ml triose phosphate dehydrogenase, 1 U/ml phosphoglucose isomerase
PH: 7
Temperature: 25 |
- [Glycerate 3-phosphate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM imidazole-HCl, 2.5 mM MgCl2, 0.1 mM NADH, 17 nkat/ml aldolase, 200 nkat/ml triose phosphate isomerase, 17 nkat/ml alpha-glycerophosphate dehydrogenase
PH: 7
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM imidazole-HCl, 2.5 mM MgCl2, 0.1 mM NADH, 17 nkat/ml aldolase, 200 nkat/ml triose phosphate isomerase, 17 nkat/ml alpha-glycerophosphate dehydrogenase
PH: 7
Temperature: 36 |
uniprot:P00511 [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S_half ^ n) ) + (S1 ^ n))) |
|
buffer: 0.1 M Imidazole-HCl, 0.2 M KCl
PH: 7.6
Temperature: 22 |
uniprot:P00511 [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S_half ^ n) ) + (S1 ^ n))) |
|
buffer: 0.1 M Imidazole-HCl, 0.2 M KCl
PH: 7.6
Temperature: 22 |
- [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM imidazole-HCl, 2.5 mM MgCl2, 0.1 mM NADH, 17 nkat/ml aldolase, 200 nkat/ml triose phosphate isomerase, 17 nkat/ml alpha-glycerophosphate dehydrogenase
PH: 7
Temperature: 36 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM imidazole-HCl, 2.5 mM MgCl2, 0.1 mM NADH, 17 nkat/ml aldolase, 200 nkat/ml triose phosphate isomerase, 17 nkat/ml alpha-glycerophosphate dehydrogenase
PH: 7
Temperature: 36 |
uniprot:Q27778 [Hycanthone]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K_2 + (S ^ n))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (K_2 + (S ^ n))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K_2 + (S ^ n))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(1 / (( (( (Km / (Vmax * S)) ) * (1 + (I / Ki))) ) + (( (1 / Vmax) ) * (1 + (I / Ki))))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
uniprot:Q27778 [Hycanthone]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (K_2 + (S ^ n))) |
|
buffer: 50 mM Mops-KOH, 100 mM KCl, 100 µM EDTA, 2 mM DTT, 0.2 mM NADH, 400 µg/ml aldolase, 40 µg/ml triosephosphate isomerase-glycerol- 3-phosphate dehydrogenase
PH: 7
Temperature: 25 |
- [3',5'-Cyclic AMP]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * M) ) / (Km + M)) |
|
buffer: 20 mM imidazole, 150 mM potassium acetate, 20 mM (NH4)2SO4, 5 mM MgCl2, 0.01 % BSA
PH: 7.4
Temperature: 37 |
uniprot:P00511 [NH4+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [NH4+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [NH4+]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [NH4+]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
uniprot:P00511 [NH4+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 40 mM sodium glycylglycine, 2 mM MgCl2, 3 mM dithiothreitol
PH: 7.2
Temperature: 28 |
- [AMP]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 50 mM KCl, 50 mM 2-Mercaptoethanol, 10 mg/ml Aldolase, 10 mg/ml alpha-Glycerolphosphate dehydrogenase and Triosephosphate isomerase mixture, 0.2 mM NADH
PH: 7
Temperature: 36 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 40 mM Tris-HCl, 100 mM KCl, 0.1 mM NADH, 0.04 U/ml Aldolase, 2.4 U/ml Triosephosphate isomerase, 0.32 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.9
Temperature: 24 |
- [Mg2+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * M) ) / (Km + M)) |
|
buffer: 40 mM Tris-HCl, 100 mM KCl, 0.1 mM NADH, 0.04 U/ml Aldolase, 2.4 U/ml Triosephosphate isomerase, 0.32 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.9
Temperature: 24 |
- [Mg2+]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 40 mM Tris-HCl, 100 mM KCl, 0.1 mM NADH, 0.04 U/ml Aldolase, 2.4 U/ml Triosephosphate isomerase, 0.32 U/ml Glycerol-3-phosphate dehydrogenase
PH: 7.9
Temperature: 24 |
- [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S1 ^ n)) ) / (( (S_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Tris-HCl, 5 mM MgCl2, 50 mM KCl, 50 mM 2-Mercaptoethanol, 10 mg/ml Aldolase, 10 mg/ml alpha-Glycerolphosphate dehydrogenase and Triosephosphate isomerase mixture, 0.2 mM NADH
PH: 7
Temperature: 36 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 100 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 100 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 100 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 100 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ h)) ) / (( (S_half ^ h) ) + (S ^ h))) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 100 µg/ml aldolase, 10 µg/ml triosephosphate isomerase, 10 µg/ml glycerol-3-phosphate dehydrogenase
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 10 µg/ml lactate dehydrogenase, 10 µg/ml pyruvate kinase, 0.2 mM phosphoenolpyruvate
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 10 µg/ml lactate dehydrogenase, 10 µg/ml pyruvate kinase, 0.2 mM phosphoenolpyruvate
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 10 µg/ml lactate dehydrogenase, 10 µg/ml pyruvate kinase, 0.2 mM phosphoenolpyruvate
PH: 6.7
Temperature: 37 |
uniprot:O15648 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM imidazole/HCl, 10 mM MgSO4, 100 mM KCl, 5 mM 2-mercaptoethanol, 0.2 mM NADH, 10 µg/ml lactate dehydrogenase, 10 µg/ml pyruvate kinase, 0.2 mM phosphoenolpyruvate
PH: 6.7
Temperature: 37 |
uniprot:P47860 [6-phosphofructokinase(Enzyme) wildtype isoenzyme Pfkp]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ h)) ) / (( (S05 ^ h) ) + (S ^ h))) |
|
buffer: 50 mM glycylglycine, 50 mM KCl, 1.5 mM MgCl2, 0.5 mM (NH4)2SO4, 0.5 mM EDTA, 5 mM DTT, 1 mg/ml BSA, 1.4 units/ml aldolase, 1.5 units/ml glycerol-3-phosphate dehydrogenase, 4.5 units/ml triosephosphate isomerase, 0.2 mM NADH
PH: 7.35
Temperature: 37 |
uniprot:P47858 [6-phosphofructokinase(Enzyme) wildtype isoenzyme Pfkm]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (Vmax * (S ^ h)) ) / (( (S05 ^ h) ) + (S ^ h))) |
|
buffer: 50 mM glycylglycine, 50 mM KCl, 1.5 mM MgCl2, 0.5 mM (NH4)2SO4, 0.5 mM EDTA, 5 mM DTT, 1 mg/ml BSA, 1.4 units/ml aldolase, 1.5 units/ml glycerol-3-phosphate dehydrogenase, 4.5 units/ml triosephosphate isomerase, 0.2 mM NADH
PH: 7.35
Temperature: 37 |
uniprot:P30835 [6-phosphofructokinase(Enzyme) wildtype isoenzyme Pfkl]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ h)) ) / (( (S05 ^ h) ) + (S ^ h))) |
|
buffer: 50 mM glycylglycine, 50 mM KCl, 1.5 mM MgCl2, 0.5 mM (NH4)2SO4, 0.5 mM EDTA, 5 mM DTT, 1 mg/ml BSA, 1.4 units/ml aldolase, 1.5 units/ml glycerol-3-phosphate dehydrogenase, 4.5 units/ml triosephosphate isomerase, 0.2 mM NADH
PH: 7.35
Temperature: 37 |
uniprot:P78985 [Citrate]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [Citrate]
D_Fructose_6_phosphate + ATP -> ADP + D_Fructose_1,6_bisphosphate |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [6-phosphofructokinase(Enzyme) wildtype]
ATP + D_Fructose_6_phosphate -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [6-phosphofructokinase(Enzyme) wildtype]
GTP + D_Fructose_6_phosphate -> GDP + D_Fructose_1,6_bisphosphate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:P78985 [6-phosphofructokinase(Enzyme) wildtype]
D_Fructose_6_phosphate + ATP -> D_Fructose_1,6_bisphosphate + ADP |
(( (( (kcat * E) ) * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 100 mM Tris-HCl, 5 mM beta-mercaptoethanol, 0.24 mM NADH, 1.5 U/ml Fructose bisphosphate aldolase, 0.8 U/ml Triose phosphate isomerase, 0.8 U/ml Glycerol 3-phosphate dehydrogenase, 0.2 mM MgCl2
PH: 7.8
Temperature: 30 |
uniprot:Q65G82 [K+]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: 20 mM Imidazole-HCl, 3.0 mM MgCl2, 1.0 mM 2-Mercaptoethanol, 0.06 mg/ml BSA, 0.16 mg/ml Aldolase, 1.28 U/ml Triosephosphate isomerase, 0.32 U/ml Glycerol-3-phosphate dehydrogenase
PH: 6.82
Temperature: 30 |
- [6-phosphofructokinase(Enzyme) mutant M-1]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * S2) ) / (Km2 + S2)) |
|
buffer: 50 mM Tris-HCl, 1 mM dithiothreitol, 0.2 mM NADH, 80 mM KCl, 5 mM MgCl2, 10 mM NH4Cl, Fructose 1,6-bisphosphate aldolase, Triosephosphate isomerase, alpha-Glycerophosphate dehydrogenase
PH: 8
Temperature: 28 |
- [6-phosphofructokinase(Enzyme) mutant M-1]
ATP + D_Fructose_6_phosphate -> ADP + D_Fructose_1,6_bisphosphate |
(( (Vmax * (S1 ^ n)) ) / (( (S1_half ^ n) ) + (S1 ^ n))) |
|
buffer: 50 mM Tris-HCl, 1 mM dithiothreitol, 0.2 mM NADH, 80 mM KCl, 5 mM MgCl2, 10 mM NH4Cl, Fructose 1,6-bisphosphate aldolase, Triosephosphate isomerase, alpha-Glycerophosphate dehydrogenase
PH: 8
Temperature: 28 |