EC: 2.5.1.1

dimethylallyltranstransferase (prenyl-diphosphate:3-methylbut-3-en-1-yl-diphosphate prenyltranstransferase)

enzyme lambda metabolic reaction experiment
- [Mg2+]

Isopentenyl_diphosphate + Dimethylallyl_diphosphate -> Geranyl_diphosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.2
Temperature: 30
- [Mg2+]

Dimethylallyl_diphosphate + Isopentenyl_diphosphate -> Geranyl_diphosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.2
Temperature: 30
- [Mg2+]

Dimethylallyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + Geranyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.7
Temperature: 37
- [Mg2+]

Dimethylallyl_diphosphate + Isopentenyl_diphosphate -> Geranyl_diphosphate + Diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.7
Temperature: 37
- [Mg2+]

Dimethylallyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + Geranyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mopso, 10% (v/v) Glycerol, 2 mM Dithiothreitol
PH: 7
Temperature: 30
- [Mg2+]

Dimethylallyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + Geranyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mopso, 10% (v/v) Glycerol, 2 mM Dithiothreitol
PH: 7
Temperature: 30
- [Mg2+]

Isopentenyl_diphosphate + Dimethylallyl_diphosphate -> Diphosphate + Geranyl_diphosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM Mopso, 10% (v/v) Glycerol, 2 mM Dithiothreitol
PH: 7
Temperature: 30
uniprot:P14324 [dimethylallyltranstransferase(Enzyme) wildtype]

Isopentenyl_diphosphate + Geranyl_diphosphate -> Diphosphate + trans,trans_Farnesyl_diphosphate		 (Vmax / (( (( (( (( (Ks * Kb) ) / (S1 * S2)) ) + (Ka / S1)) ) + (Kb / S2)) ) + 1)) buffer: 10 mM Tris-HCl, 2 mM MgCl2, 2 mM DTT
PH: 7.8
Temperature: 37
uniprot:P14324 [Mn2+]

Isopentenyl_diphosphate + Geranyl_diphosphate -> Diphosphate + trans,trans_Farnesyl_diphosphate		 (( (Vmax * M) ) / (Km_M + M)) buffer: 10 mM Tris-HCl, 2 mM DTT
PH: 7.8
Temperature: 37
uniprot:P14324 [Mg2+]

Geranyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + trans,trans_Farnesyl_diphosphate		 (( (Vmax * M) ) / (Km_M + M)) buffer: 10 mM Tris-HCl, 2 mM DTT
PH: 7.8
Temperature: 37
uniprot:P14324 [dimethylallyltranstransferase(Enzyme) wildtype]

Isopentenyl_diphosphate + Geranyl_diphosphate -> Diphosphate + trans,trans_Farnesyl_diphosphate		 (( (Vmax * S1) ) / (Km1 + S1)) buffer: 10 mM Tris-HCl, 2 mM MgCl2, 2 mM DTT
PH: 7.8
Temperature: 37
uniprot:P14324 [dimethylallyltranstransferase(Enzyme) wildtype]

Isopentenyl_diphosphate + Geranyl_diphosphate -> trans,trans_Farnesyl_diphosphate + Diphosphate		 (( (Vmax * S2) ) / (Km2 + S2)) buffer: 10 mM Tris-HCl, 2 mM MgCl2, 2 mM DTT
PH: 7.8
Temperature: 37
uniprot:P14324 [dimethylallyltranstransferase(Enzyme) wildtype]

Geranyl_diphosphate + Isopentenyl_diphosphate -> Diphosphate + trans,trans_Farnesyl_diphosphate		 (( (Vmax * S1) ) / (Km1 + S1)) buffer: 10 mM Tris-HCl, 2 mM MgCl2
PH: 7.8
Temperature: 37
uniprot:P14324 [dimethylallyltranstransferase(Enzyme) wildtype]

Isopentenyl_diphosphate + Geranyl_diphosphate -> trans,trans_Farnesyl_diphosphate + Diphosphate		 (( (Vmax * S2) ) / (Km2 + S2)) buffer: 10 mM Tris-HCl, 2 mM MgCl2
PH: 7.8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-5120 geranylgeranyldiphosphate biosynthesis
BioCyc:META_PWY-6859 all-trans-farnesol biosynthesis
BioCyc:META_PWY-7182 linalool biosynthesis I
BioCyc:YEAST_HEXPPSYN-PWY-1 hexaprenyl diphosphate biosynthesis
BioCyc:ARA_PWYQT-4449 polyisoprenoid biosynthesis
BioCyc:ARA_PWY-5120 geranylgeranyl diphosphate biosynthesis
BioCyc:MOUSE_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-5122 geranyl diphosphate biosynthesis
BioCyc:META_PWY-7709 (3R)-linalool biosynthesis
BioCyc:META_PWY-5120 geranylgeranyl diphosphate biosynthesis
BioCyc:META_PWY-7492 paspaline biosynthesis
BioCyc:META_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:YEAST_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:MOUSE_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:HUMAN_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:META_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
BioCyc:META_PWY-5863 superpathway of phylloquinol biosynthesis
BioCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
BioCyc:ARA_PWY-5122 geranyl diphosphate biosynthesis
BioCyc:ARA_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:META_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
BioCyc:META_PWY-7410 ipsdienol biosynthesis
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:YEAST_PWY-5122 geranyl diphosphate biosynthesis
BioCyc:YEAST_PWY-5120 geranylgeranyldiphosphate biosynthesis
BioCyc:MTBH37RV_PWY-5120 geranylgeranyl diphosphate biosynthesis
PlantCyc:ARA_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:CANNUUM_PWY-5122 geranyl diphosphate biosynthesis
PlantCyc:CANNUUM_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:CANNUUM_PWY2OL-4 superpathway of linalool biosynthesis
PlantCyc:CANNUUM_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:RUBBERTREE_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:RUBBERTREE_PWY-5122 geranyl diphosphate biosynthesis
PlantCyc:RUBBERTREE_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:RUBBERTREE_PWY-5120 geranylgeranyl diphosphate biosynthesis
PlantCyc:PLANT_PWY-7182 linalool biosynthesis I
PlantCyc:ARA_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:CANNUUM_PWY-7141 (3S)-linalool biosynthesis
PlantCyc:CANNUUM_PWY-5120 geranylgeranyl diphosphate biosynthesis
PlantCyc:RUBBERTREE_PWYQT-4449 polyisoprenoid biosynthesis
PlantCyc:RUBBERTREE_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:CANNUUM_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:PLANT_PWYQT-4449 polyisoprenoid biosynthesis
PlantCyc:PLANT_PWY-5122 geranyl diphosphate biosynthesis
PlantCyc:PLANT_PWY-7709 (3R)-linalool biosynthesis
PlantCyc:ARA_PWY-5120 geranylgeranyl diphosphate biosynthesis
PlantCyc:CANNUUM_PWY-7182 linalool biosynthesis I
PlantCyc:PLANT_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:RUBBERTREE_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:ARA_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:CANNUUM_PWYQT-4449 polyisoprenoid biosynthesis
PlantCyc:PLANT_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:ARA_PWYQT-4449 polyisoprenoid biosynthesis
PlantCyc:ARA_PWY-5122 geranyl diphosphate biosynthesis
PlantCyc:ARA_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:CANNUUM_PWY-7709 (3R)-linalool biosynthesis
PlantCyc:CANNUUM_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:PLANT_PWY-5863 superpathway of phylloquinol biosynthesis
PlantCyc:PLANT_PWY-5120 geranylgeranyl diphosphate biosynthesis
PlantCyc:SWEETWORMWOOD_PWY-7141 (3S)-linalool biosynthesis
PlantCyc:SWEETWORMWOOD_PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PlantCyc:SWEETWORMWOOD_PWY-7709 (3R)-linalool biosynthesis
PlantCyc:SWEETWORMWOOD_PWY-5122 geranyl diphosphate biosynthesis
PlantCyc:SWEETWORMWOOD_PWY2OL-4 superpathway of linalool biosynthesis
PlantCyc:SWEETWORMWOOD_PWY-5123 trans, trans-farnesyl diphosphate biosynthesis
PlantCyc:SWEETWORMWOOD_PWY-5910 superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate)
PlantCyc:SWEETWORMWOOD_PWY-7182 linalool biosynthesis I
Reactome:R-HSA-9969896 Lanosterol biosynthesis
Reactome:R-MMU-9969896 Lanosterol biosynthesis
Reactome:R-SPO-9969896 Lanosterol biosynthesis
Reactome:R-BTA-9969896 Lanosterol biosynthesis
Reactome:R-DDI-9969896 Lanosterol biosynthesis
Reactome:R-RNO-9969896 Lanosterol biosynthesis
Reactome:R-SCE-9969896 Lanosterol biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-191273 Cholesterol biosynthesis
Reactome:R-DDI-191273 Cholesterol biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-8957322 Metabolism of steroids
Reactome:R-DDI-8957322 Metabolism of steroids
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-SCE-1430728 Metabolism
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
WikiPathways:WP4190 Mevalonate arm of cholesterol biosynthesis pathway
PathBank:SMP0120794 Mevalonic Aciduria
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0087326 Steroid Biosynthesis
PathBank:SMP0012042 Epoxysqualene Biosynthesis
PathBank:SMP0120685 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120769 Hypercholesterolemia
PathBank:SMP0120793 Hyper-IgD Syndrome
PathBank:SMP0121055 Mevalonate Pathway
PathBank:SMP0121210 Terpenoid Backbone Biosynthesis
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0120684 CHILD Syndrome
PathBank:SMP0120692 Desmosterolosis
PathBank:SMP0120732 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120792 Cholesteryl Ester Storage Disease
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0121208 MEP/DOXP Pathway
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0120735 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120795 Wolman Disease
PathBank:SMP0121209 Mevalonate Pathway
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002384 Terpenoid Backbone Biosynthesis
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)
PathBank:SMP0012049 Farnesene Biosynthesis