EC: 2.3.3.1

citrate (Si)-synthase (acetyl-CoA:oxaloacetate C-acetyltransferase [thioester-hydrolysing, (pro-S)-carboxymethyl forming])

enzyme lambda metabolic reaction experiment
- [citrate (Si)-synthase(Enzyme) wildtype]

H2O + Acetyl_CoA + Oxaloacetate -> Citrate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM KH2PO4-KOH, 10 mM MgCl2, 50 mM KCl, 0.1 mM DTNB
PH: 8
Temperature: 30
- [citrate (Si)-synthase(Enzyme) wildtype]

Acetyl_CoA + Oxaloacetate + H2O -> Citrate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 25 mM KH2PO4-KOH, 10 mM MgCl2, 50 mM KCl, 0.1 mM DTNB
PH: 8
Temperature: 30
- [citrate (Si)-synthase(Enzyme) wildtype]

Oxaloacetate + Acetyl_CoA + H2O -> Coenzyme_A + Citrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 83 µM DTNB
PH: 8.1
Temperature: 25
- [citrate (Si)-synthase(Enzyme) wildtype]

Oxaloacetate + H2O + Acetyl_CoA -> Coenzyme_A + Citrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 83 µM DTNB
PH: 8.1
Temperature: 25
- [ATP]

Oxaloacetate + Acetyl_CoA + H2O -> Coenzyme_A + Citrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 83 µM DTNB
PH: 8.1
Temperature: 25
- [ATP]

Oxaloacetate + H2O + Acetyl_CoA -> Coenzyme_A + Citrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 0.1 M potassium phosphate, 83 µM DTNB
PH: 8.1
Temperature: 25
uniprot:P00890 [citrate (Si)-synthase(Enzyme) wildtype]

H2O + Acetyl_CoA + Oxaloacetate -> Citrate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 0.1 mM 5,5′-dithio-bis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 36
uniprot:P00890 [citrate (Si)-synthase(Enzyme) mutant S462A]

H2O + Acetyl_CoA + Oxaloacetate -> Citrate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 0.1 mM 5,5′-dithio-bis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 36
uniprot:P00890 [citrate (Si)-synthase(Enzyme) mutant S462E]

Acetyl_CoA + Oxaloacetate + H2O -> Citrate + Coenzyme_A		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 0.1 mM 5,5′-dithio-bis(2-nitrobenzoic acid)
PH: 7.4
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:MOUSE_TCA-1 TCA cycle
BioCyc:MOUSE_PWY-5690-1 TCA cycle variation III (eukaryotic)
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:HUMAN_PWY66-398 TCA cycle
BioCyc:META_PWY66-398 TCA cycle III (animals)
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:YEAST_PWY-5690 TCA cycle II (plants and fungi)
Reactome:R-DRE-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SSC-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-BTA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-HSA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-BTA-71403 Citric acid cycle (TCA cycle)
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-372987 The tricarboxylic acid cycle
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-XTR-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-XTR-71403 Citric acid cycle (TCA cycle)
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DRE-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SCE-71403 Citric acid cycle (TCA cycle)
Reactome:R-XTR-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SSC-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SSC-71403 Citric acid cycle (TCA cycle)
WikiPathways:WP5220 Metabolic reprogramming in pancreatic cancer
PathBank:SMP0120830 Fumarase Deficiency
PathBank:SMP0120834 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0120613 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000548 Mitochondrial Complex II Deficiency
PathBank:SMP0002295 The Oncogenic Action of Fumarate
PathBank:SMP0002358 The Oncogenic Action of L-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087254 Transfer of Acetyl Groups into Mitochondria
PathBank:SMP0087270 Warburg Effect
PathBank:SMP0087274 Citric Acid Cycle
PathBank:SMP0120829 Congenital Lactic Acidosis
PathBank:SMP0120833 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0108760 Glutaminolysis and Cancer
PathBank:SMP0108771 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0120612 Mitochondrial Complex II Deficiency
PathBank:SMP0120922 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120946 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120970 LPS and Citrate Signaling and Inflammation
PathBank:SMP0000547 Fumarase Deficiency
PathBank:SMP0000551 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0002292 The Oncogenic Action of Succinate
PathBank:SMP0120832 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0108758 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0108773 Glutaminolysis and Cancer
PathBank:SMP0120610 Congenital Lactic Acidosis
PathBank:SMP0120614 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0000057 Citric Acid Cycle
PathBank:SMP0000549 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0002359 The Oncogenic Action of D-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087163 Citric Acid Cycle
PathBank:SMP0100046 LPS and Citrate Signaling and Inflammation
PathBank:SMP0120831 Mitochondrial Complex II Deficiency
PathBank:SMP0120611 Fumarase Deficiency
PathBank:SMP0120615 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0000546 Congenital Lactic Acidosis
PathBank:SMP0000550 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0002291 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0002304 TCA Cycle
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087347 Transfer of Acetyl Groups into Mitochondria
PathBank:SMP0087363 Warburg Effect
PathBank:SMP0063477 Citric Acid Cycle
PathBank:SMP0063681 Transfer of Acetyl Groups into Mitochondria