EC: 6.3.5.1

NAD+ synthase (glutamine-hydrolysing) (deamido-NAD+:L-glutamine amido-ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn1 C-terminal His6-tagged]

ATP + L_Glutamine + H2O + Deamino_NAD+ -> NAD+ + Diphosphate + L_Glutamate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 7.5
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn1 C-terminal His6-tagged]

ATP + H2O + Deamino_NAD+ + L_Glutamine -> Diphosphate + L_Glutamate + AMP + NAD+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 7.5
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn1 C-terminal His6-tagged]

ATP + L_Glutamine + Deamino_NAD+ + H2O -> AMP + NAD+ + Diphosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-Cl, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 7.5
Temperature: 37
- [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn2 C-terminal His6-tagged]

Deamino_NAD+ + ATP + L_Glutamine + H2O -> L_Glutamate + NAD+ + Diphosphate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin
PH: 8.8
Temperature: 37
- [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn2 C-terminal His6-tagged]

H2O + L_Glutamine + ATP + Deamino_NAD+ -> Diphosphate + NAD+ + AMP + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin
PH: 8.8
Temperature: 37
- [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn2 C-terminal His6-tagged]

L_Glutamine + H2O + Deamino_NAD+ + ATP -> L_Glutamate + Diphosphate + NAD+ + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin
PH: 8.8
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) mutant NADsyn1 C175S C-terminal His6-tagged]

Deamino_NAD+ + NH4+ + ATP -> Diphosphate + NAD+ + AMP + H+		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 8.8
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn1 C-terminal His6-tagged]

ATP + Deamino_NAD+ + NH4+ -> NAD+ + AMP + H+ + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 8.8
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) mutant NADsyn1 C175S C-terminal His6-tagged]

NH4+ + ATP + Deamino_NAD+ -> H+ + Diphosphate + NAD+ + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 8.8
Temperature: 37
uniprot:Q6IA69 [NAD+ synthase (glutamine-hydrolysing)(Enzyme) mutant NADsyn1 C175S C-terminal His6-tagged]

Deamino_NAD+ + ATP + NH4+ -> NAD+ + Diphosphate + H+ + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin, 2 mM dithiothreitol
PH: 8.8
Temperature: 37
- [NAD+ synthase (glutamine-hydrolysing)(Enzyme) wildtype NADsyn2 C-terminal His6-tagged]

NH4+ + ATP + Deamino_NAD+ -> NAD+ + H+ + AMP + Diphosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES, 56 mM KCl, 5 mM MgCl2, 0.2 µg/µl bovine serum albumin
PH: 8.8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:YEAST_PWY3O-4107 NAD salvage pathway
BioCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
BioCyc:MTBH37RV_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
BioCyc:YEAST_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:ARA_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:MTBH37RV_PYRIDNUCSAL-PWY NAD salvage pathway I (PNC VI cycle)
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:MTBCDC1551_PYRIDNUCSYN-PWY NAD biosynthesis I (from aspartate)
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MTBH37RV_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_PWY-5653 NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_PYRNUCYC-PWY pyridine nucleotide cycling
PlantCyc:ARA_PWY-5381 pyridine nucleotide cycling (plants)
PlantCyc:ARA_PYRIDNUCSYN-PWY NAD de novo biosynthesis I (from aspartate)
PlantCyc:ARA_PWY3O-4107 NAD salvage pathway V (PNC V cycle)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-196807 Nicotinate metabolism
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DME-196807 Nicotinate metabolism
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-RNO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-196807 Nicotinate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-196807 Nicotinate metabolism
Reactome:R-DDI-196807 Nicotinate metabolism
Reactome:R-HSA-196807 Nicotinate metabolism
Reactome:R-MMU-196807 Nicotinate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-196807 Nicotinate metabolism
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-BTA-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DME-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0087169 Glutamate Metabolism
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0087334 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0063643 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0087241 Nicotinate and Nicotinamide Metabolism
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0002338 NAD Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000048 Nicotinate and Nicotinamide Metabolism