EC: 6.3.4.14

biotin carboxylase ([biotin carboxyl-carrier protein]-biotin-N6-L-lysine:hydrogencarbonate ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

Hydrogencarbonate + Holo_[carboxylase] + ATP -> ADP + Carboxybiotin_carboxyl_carrier_protein + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

ATP + Hydrogencarbonate + H2O -> Phosphate + CO2 + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E211A]

ATP + Hydrogencarbonate + H2O -> CO2 + Phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant N290A]

ATP + Hydrogencarbonate + H2O -> ADP + Phosphate + CO2		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E288A]

Hydrogencarbonate + ATP + H2O -> CO2 + Phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R292A]

ATP + H2O + Hydrogencarbonate -> ADP + CO2 + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

Hydrogencarbonate + H2O + ATP -> CO2 + Phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R292A]

H2O + ATP + Hydrogencarbonate -> CO2 + ADP + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant N290A]

ATP + H2O + Hydrogencarbonate -> ADP + CO2 + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E211A]

Hydrogencarbonate + H2O + ATP -> Phosphate + ADP + CO2		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E288A]

H2O + ATP + Hydrogencarbonate -> CO2 + Phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R292A]

ATP + Holo_[carboxylase] + Hydrogencarbonate -> ADP + Carboxybiotin_carboxyl_carrier_protein + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant N290A]

Hydrogencarbonate + ATP + Holo_[carboxylase] -> ADP + Carboxybiotin_carboxyl_carrier_protein + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E288A]

ATP + Holo_[carboxylase] + Hydrogencarbonate -> ADP + Carboxybiotin_carboxyl_carrier_protein + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E211A]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> ADP + Phosphate + Carboxybiotin_carboxyl_carrier_protein		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant N290A]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (( (V1 * A) ) / (K1 + A)) ) + (( (V2 * A) ) / (K2 + A))) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E211A]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E288A]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R292A]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

ATP + Holo_[carboxylase] + Hydrogencarbonate -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

Hydrogencarbonate + H2O + ATP -> Phosphate + CO2 + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

ATP + Hydrogencarbonate + H2O -> Phosphate + CO2 + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

ATP + H2O + Hydrogencarbonate -> CO2 + Phosphate + ADP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E288A]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R292A]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant E211A]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant N290A]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2, 50 mM biotin
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> ADP + Phosphate + Carboxybiotin_carboxyl_carrier_protein		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

ATP + Hydrogencarbonate + Holo_[carboxylase] -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (V * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

Holo_[carboxylase] + Hydrogencarbonate + ATP -> Phosphate + Carboxybiotin_carboxyl_carrier_protein + ADP		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

Hydrogencarbonate + Holo_[carboxylase] + ATP -> ADP + Phosphate + Carboxybiotin_carboxyl_carrier_protein		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338S]

Holo_[carboxylase] + Hydrogencarbonate + ATP -> Phosphate + ADP + Carboxybiotin_carboxyl_carrier_protein		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant R338Q]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> ADP + Carboxybiotin_carboxyl_carrier_protein + Phosphate		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant M169K]

H2O + ATP + Hydrogencarbonate -> Phosphate + CO2 + ADP		 (( (V * (A ^ n)) ) / (K + (A ^ n))) buffer: 100 mM HEPES, 0.5 mM phosphoenolpyruvate, 0.4 mM NADH, 23 U pyruvate kinase, 35 U lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

ADP + Carbamoyl_phosphate -> Carbamate + ATP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant K238Q]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant C230A]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

ATP + H2O + Hydrogencarbonate -> CO2 + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [N-Ethylmaleimide]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> Carboxybiotin_carboxyl_carrier_protein + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 50 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [N-Ethylmaleimide]

Hydrogencarbonate + ATP + Holo_[carboxylase] -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) wildtype]

ATP + Holo_[carboxylase] + Hydrogencarbonate -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant C230A]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> Carboxybiotin_carboxyl_carrier_protein + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant C230A]

Hydrogencarbonate + ATP + H2O -> CO2 + ADP + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant K238Q]

H2O + Hydrogencarbonate + ATP -> ADP + CO2 + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 8 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.2 mM NADH, 20 U/ml pyruvate kinase, 36 U/ml lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant K238Q]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [biotin carboxylase(Enzyme) mutant C230A]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 5 U hexokinase, 5 U glucose-6-phosphate dehydrogenase, 8 mM MgCl2
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

H2O + ATP + Hydrogencarbonate -> ADP + Phosphate + CO2		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

H2O + Hydrogencarbonate + ATP -> ADP + CO2 + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

Hydrogencarbonate + H2O + ATP -> CO2 + Phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

ATP + Holo_[carboxylase] + Hydrogencarbonate -> Phosphate + Carboxybiotin_carboxyl_carrier_protein + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> ADP + Phosphate + Carboxybiotin_carboxyl_carrier_protein		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Hydrogencarbonate + ATP + H2O -> Phosphate + CO2 + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

H2O + Hydrogencarbonate + ATP -> CO2 + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

H2O + ATP + Hydrogencarbonate -> CO2 + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Hydrogencarbonate + H2O + ATP -> ADP + CO2 + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

ATP + H2O + Hydrogencarbonate -> ADP + CO2 + Phosphate		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Biotin]

ATP + Hydrogencarbonate + H2O -> CO2 + Phosphate + ADP		 (( (Vmax * B) ) / (Km + B)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Holo_[carboxylase] + ATP + Hydrogencarbonate -> Phosphate + ADP + Carboxybiotin_carboxyl_carrier_protein		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

ATP + Hydrogencarbonate + Holo_[carboxylase] -> Carboxybiotin_carboxyl_carrier_protein + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM Phosphoenolpyruvate, 0.2 mM NADH, 10 U/ml Pyruvate kinase, 18 U/ml Lactate dehydrogenase
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Carbamoyl_phosphate + ADP -> Carbamate + ATP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

Carbamoyl_phosphate + ADP -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25
uniprot:P24182 [Mg2+]

ADP + Carbamoyl_phosphate -> ATP + Carbamate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM HEPES, 0.5 mM glucose, 0.4 mM NADP, 2.5 U/ml Hexokinase, 2.5 U/ml Glucose 6-phosphate dehydrogenase, 100 mM KCl
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:ECO_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:YEAST_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:MTBH37RV_PROPIONMET-PWY propanoyl CoA degradation I
BioCyc:MTBH37RV_PWY0-1264 biotin-carboxyl carrier protein assembly
BioCyc:BSUB_PWY0-1264 biotin-carboxyl carrier protein assembly
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000818 Palmitate Biosynthesis
PathBank:SMP0000819 Fatty Acid Elongation (Saturated)
PathBank:SMP0000919 Fatty Acid Biosynthesis
PathBank:SMP0002058 Palmitate Biosynthesis 2
PathBank:SMP0002079 Biotin-Carboxyl Carrier Protein Assembly
PlantCyc:PLANT_PWY0-1264 biotin-carboxyl carrier protein assembly
PlantCyc:ARA_PWY0-1264 biotin-carboxyl carrier protein assembly
Reactome:R-DDI-75105 Fatty acyl-CoA biosynthesis
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-196780 Biotin transport and metabolism
Reactome:R-SCE-75105 Fatty acyl-CoA biosynthesis
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-75105 Fatty acyl-CoA biosynthesis
Reactome:R-DDI-8978868 Fatty acid metabolism
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-8978868 Fatty acid metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-196780 Biotin transport and metabolism
Reactome:R-SPO-8978868 Fatty acid metabolism
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-556833 Metabolism of lipids
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-196780 Biotin transport and metabolism
PathBank:SMP0002304 TCA Cycle
PathBank:SMP0002355 Pyruvate Metabolism