EC: 3.6.1.23

dUTP diphosphatase (dUTP nucleotidohydrolase)

enzyme lambda metabolic reaction experiment
- [Mg2+]

dUTP + H2O -> Diphosphate + dUMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-hydrochloride, 2 mM 2-mercaptoethanol, 0.1% (w/v) bovine serum albumin
PH: 8
Temperature: 37
uniprot:P33316 [Mg2+]

dUTP + H2O -> Diphosphate + dUMP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris-HCl, 2 mM Mercaptoethanol, 0.1% Bovine serum albumin
PH: 7.5
Temperature: 37
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

dUTP + H2O -> Diphosphate + dUMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

dTTP + H2O -> dTMP + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

dATP + H2O -> dAMP + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

dUDP + H2O -> dUMP + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

dGTP + H2O -> dGMP + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

H2O + dCTP -> dCMP + Diphosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25
uniprot:O15826 [dUTP diphosphatase(Enzyme) wildtype]

UTP + H2O -> Diphosphate + UMP		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.5 mM Cresol Red, 25 mM BICINE, 25 mM MgCl2, 0.01% C12E8
PH: 8
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ECO_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:ECO_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBH37RV_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:MTBH37RV_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBCDC1551_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:MTBCDC1551_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ECOL316407_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOL316407_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:PDIF272563_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:PDIF272563_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:BTHE_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBH37RV_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBCDC1551_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HPY_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOO157_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:BTHE_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:BTHE_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:CORYNE_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:CORYNE_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:CORYNE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:META_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:ECO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOO157_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:AGRO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:META_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECO_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:ARA_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
BioCyc:MTBH37RV_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:MTBH37RV_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:ECOO157_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:CAULO_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
BioCyc:AGRO_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
BioCyc:AGRO_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
BioCyc:CORYNE_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PathBank:SMP0000959 Pyrimidine Metabolism
PlantCyc:ARA_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7206 pyrimidine deoxyribonucleotides dephosphorylation
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7184 pyrimidine deoxyribonucleotides de novo biosynthesis I
PlantCyc:ARA_PWY-7187 pyrimidine deoxyribonucleotides de novo biosynthesis II
PlantCyc:ARA_PWY-6545 pyrimidine deoxyribonucleotides de novo biosynthesis III
PlantCyc:PLANT_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
Reactome:R-HSA-9609690 HCMV Early Events
Reactome:R-HSA-9609646 HCMV Infection
Reactome:R-HSA-9824446 Viral Infection Pathways
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-SPO-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DDI-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-SCE-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SCE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-MMU-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-RNO-499943 Interconversion of nucleotide di- and triphosphates
PathBank:SMP0120678 beta-Ureidopropionase Deficiency
PathBank:SMP0120750 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0121324 Pyrimidine Metabolism
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0120693 Dihydropyrimidinase Deficiency
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0087340 Pyrimidine Metabolism
PathBank:SMP0120727 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000172 beta-Ureidopropionase Deficiency