EC: 2.7.4.14

UMP/CMP kinase (ATP:CMP(UMP) phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype His-tagged]

UMP + ATP -> ADP + UDP
(( (V * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris/HCl, 50 mM KCl, 5 mM MgCl2, 0.2 mM NADH, 10 mM Dithiothreitol, 1 mM Phosphoenolpyruvate, 4 U Pyruvate kinase, 4 U Lactate dehydrogenase, 4 U NDP kinase from Dictyostelium
PH: 7.4
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione S-transferase]

UMP + ATP -> UDP + ADP
(( (V * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris/HCl, 50 mM KCl, 5 mM MgCl2, 0.2 mM NADH, 10 mM Dithiothreitol, 1 mM Phosphoenolpyruvate, 4 U Pyruvate kinase, 4 U Lactate dehydrogenase, 4 U NDP kinase from Dictyostelium
PH: 7.4
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype His-tagged]

CMP + ATP -> ADP + CDP
(( (V * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris/HCl, 50 mM KCl, 5 mM MgCl2, 0.2 mM NADH, 10 mM Dithiothreitol, 1 mM Phosphoenolpyruvate, 4 U Pyruvate kinase, 4 U Lactate dehydrogenase, 4 U NDP kinase from Dictyostelium
PH: 7.4
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione S-transferase]

CMP + ATP -> CDP + ADP
(( (V * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris/HCl, 50 mM KCl, 5 mM MgCl2, 0.2 mM NADH, 10 mM Dithiothreitol, 1 mM Phosphoenolpyruvate, 4 U Pyruvate kinase, 4 U Lactate dehydrogenase, 4 U NDP kinase from Dictyostelium
PH: 7.4
Temperature: 37
uniprot:P20425 [UMP/CMP kinase(Enzyme) wildtype]

UMP + ATP -> ADP + UDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM beta-mercaptoethanol, Pyruvate kinase
PH: 7.4
Temperature: 30
uniprot:P20425 [UMP/CMP kinase(Enzyme) wildtype]

UMP + ATP -> ADP + UDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM beta-mercaptoethanol, Pyruvate kinase
PH: 7.4
Temperature: 30
uniprot:P20425 [UMP/CMP kinase(Enzyme) wildtype]

ATP + CMP -> ADP + CDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM beta-mercaptoethanol, Pyruvate kinase
PH: 7.4
Temperature: 30
uniprot:P20425 [UMP/CMP kinase(Enzyme) wildtype]

ATP + CMP -> ADP + CDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 1 mM beta-mercaptoethanol, Pyruvate kinase
PH: 7.4
Temperature: 30
uniprot:P30085 [Mg2+]

ATP + CMP -> ADP + CDP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

ATP + dCMP -> dCDP + ADP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

dCMP + ATP -> dCDP + ADP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

ATP + dCMP -> ADP + dCDP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

dCMP + ATP -> dCDP + ADP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

ATP + CMP -> ADP + CDP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [Mg2+]

CMP + ATP -> ADP + CDP
(( (V * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM NaF, 2 mM DTT, creatine phosphate, creatine kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype recombinant 228 aa]

UMP + ATP -> UDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

UMP + ATP -> ADP + UDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + CMP -> CDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype recombinant 228 aa]

ATP + CMP -> ADP + CDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + dCMP -> dCDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + dCMP -> ADP + dCDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + dCMP -> dCDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype recombinant 228 aa]

ATP + dCMP -> ADP + dCDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

UMP + ATP -> UDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

CMP + ATP -> CDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + 1_beta_D_Arabinofuranosylcytosine_5'_monophosphate -> 1_beta_D_Arabinofuranosylcytosine_5'_diphosphate + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + Gemcitabine_monophosphate -> Gemcitabine_diphosphate + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + D_ddCMP -> D_ddCDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

L_ddCMP + ATP -> L_ddCDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

L_OddCMP + ATP -> ADP + L_OddCDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + L_SddCMP -> ADP + L_SddCDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

L_FSddCMP + ATP -> L_FSddCDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype]

ATP + L_Fd4CMP -> ADP + L_Fd4CDP
(( (Vmax * S) ) / (Km + S)) buffer: 0.02 M Tris-HCl, 5.4 U/ml creatine kinase, 3 mM phospho-creatine, 1 % albumin, 1 U/ml NDP kinase
PH: 7.5
Temperature: 37
uniprot:Q5EBM0 [UMP/CMP kinase(Enzyme) wildtype CMPK2delta21-6His]

ATP + UMP -> UDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q5EBM0 [UMP/CMP kinase(Enzyme) wildtype CMPK2delta21-6His]

ATP + CMP -> CDP + ADP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q5EBM0 [UMP/CMP kinase(Enzyme) wildtype CMPK2delta21-6His]

ATP + dCMP -> ADP + dCDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 6 mM MgCl2
PH: 8
Temperature: 37
uniprot:Q5EBM0 [UMP/CMP kinase(Enzyme) wildtype CMPK2delta21-6His]

ATP + dUMP -> ADP + dUDP
(( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 5 mM MgCl2
PH: 8
Temperature: 37
- [UMP/CMP kinase(Enzyme) wildtype]

dCMP + ATP -> dCDP + ADP
(( (Vm * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCL, 2 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.2 mM NADH, 2 units lactate dehydrogenase, 2 units pyruvate kinase, 2 units CMP kinase
PH: 7.4
Temperature: 30
- [UMP/CMP kinase(Enzyme) wildtype]

ATP + CMP -> ADP + CDP
(( (Vm * S) ) / (( (Km + S) ) + (( (S ^ 2) ) / Ki))) buffer: 50 mM Tris-HCl, 50 mM KCL, 2 mM MgCl2, 1 mM Phosphoenolpyruvate, 0.2 mM NADH, 2 units lactate dehydrogenase, 2 units pyruvate kinase, 2 units CMP kinase
PH: 7.4
Temperature: 30
uniprot:P30085 [1,4-Dithiothreitol]

ATP + dCMP -> dCDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [1,4-Dithiothreitol]

UMP + ATP -> UDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [1,4-Dithiothreitol]

ATP + dUMP -> dUDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [1,4-Dithiothreitol]

CMP + ATP -> CDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione-S-transferase]

ATP + dCMP -> dCDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione-S-transferase]

ATP + UMP -> ADP + UDP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione-S-transferase]

dUMP + ATP -> dUDP + ADP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [UMP/CMP kinase(Enzyme) wildtype fusion protein to glutathione-S-transferase]

ATP + CMP -> ADP + CDP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [1,4-Dithiothreitol]

2',2'_Difluorodeoxycytidine_monophosphate + ATP -> ADP + 2',2'_Difluorodeoxycytidine_diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37
uniprot:P30085 [1,4-Dithiothreitol]

1_beta_D_Arabinofuranosylcytosine_5'_monophosphate + ATP -> ADP + 1_beta_D_Arabinofuranosylcytosine_5'_diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethylamine phosphate, 5% (v/v) methanol
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-7205 CMP phosphorylation
BioCyc:META_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:YEAST_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:ARA_PWY-5687 pyrimidine ribonucleotides interconversion
BioCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:HUMAN_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:HUMAN_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:META_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
BioCyc:META_PWY-7208 superpathway of pyrimidine nucleobases salvage
BioCyc:META_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
BioCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:HUMAN_PWY-7205 CMP phosphorylation
BioCyc:META_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:YEAST_PWY-7205 CMP phosphorylation
BioCyc:YEAST_PYRIMID-RNTSYN-PWY de novo biosynthesis of pyrimidine ribonucleotides
BioCyc:ARA_PWY-7205 CMP phosphorylation
BioCyc:ARA_PWY-7176 UTP and CTP de novo biosynthesis
BioCyc:ARA_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:ARA_PWY-7176 UTP and CTP de novo biosynthesis
PlantCyc:ARA_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
PlantCyc:PLANT_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:PLANT_PWY-7197 pyrimidine deoxyribonucleotide phosphorylation
PlantCyc:ARA_PWY-7208 superpathway of pyrimidine nucleobases salvage
PlantCyc:PLANT_PWY-5687 pyrimidine ribonucleotides interconversion
PlantCyc:PLANT_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
PlantCyc:PLANT_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:PLANT_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:PLANT_PWY-7176 UTP and CTP de novo biosynthesis
PlantCyc:ARA_PWY0-162 superpathway of pyrimidine ribonucleotides de novo biosynthesis
PlantCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
PlantCyc:ARA_PWY-7205 CMP phosphorylation
PlantCyc:ARA_PWY-5687 pyrimidine ribonucleotides interconversion
PlantCyc:PLANT_PWY-7211 superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-SPO-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-XTR-15869 Nucleotide metabolism
Reactome:R-XTR-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-DDI-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-15869 Nucleotide metabolism
Reactome:R-SSC-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DRE-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SCE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-XTR-1430728 Metabolism
Reactome:R-DRE-15869 Nucleotide metabolism
Reactome:R-DRE-499943 Interconversion of nucleotide di- and triphosphates
Reactome:R-DDI-1430728 Metabolism
PathBank:SMP0120862 Congenital Disorder of Glycosylation CDG-IId
PathBank:SMP0000444 Lactose Synthesis
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000580 GLUT-1 Deficiency Syndrome
PathBank:SMP0000649 Lamivudine Metabolism Pathway
PathBank:SMP0087229 Lactose Synthesis
PathBank:SMP0012077 Pyrimidine Deoxyribonucleosides Salvage
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000446 Gemcitabine Action Pathway
PathBank:SMP0000579 Congenital Disorder of Glycosylation CDG-IId
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0087448 Lactose Synthesis
PathBank:SMP0120863 GLUT-1 Deficiency Syndrome
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0000603 Gemcitabine Metabolism Pathway
PathBank:SMP0087323 Lactose Synthesis