EC: 1.14.19.20

DELTA7-sterol 5(6)-desaturase (DELTA7-sterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating)

enzyme lambda metabolic reaction experiment
uniprot:Q39208 [NADH]

O2 + 5alpha_Cholest_7_en_3beta_ol + 2 H+ + 2 Ferrocytochrome_b5 -> 2 H2O + 2 Ferricytochrome_b5 + Cholesta_5,7_dien_3beta_ol		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

2 H+ + 2 Ferrocytochrome_b5 + O2 + 5alpha_Cholest_7_en_3beta_ol -> Cholesta_5,7_dien_3beta_ol + 2 H2O + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:P32353 [NADH]

2 Ferrocytochrome_b5 + 5alpha_Cholest_7_en_3beta_ol + 2 H+ + O2 -> 2 Ferricytochrome_b5 + 2 H2O + Cholesta_5,7_dien_3beta_ol		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

5alpha_Cholest_7_en_3beta_ol + O2 + 2 H+ + 2 Ferrocytochrome_b5 -> Cholesta_5,7_dien_3beta_ol + 2 H2O + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

O2 + 5alpha_Cholest_7_en_3beta_ol + 2 H+ + 2 Ferrocytochrome_b5 -> 2 Ferricytochrome_b5 + 2 H2O + Cholesta_5,7_dien_3beta_ol		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

O2 + 5alpha_Cholest_7_en_3beta_ol + 2 H+ + 2 Ferrocytochrome_b5 -> 2 Ferricytochrome_b5 + 2 H2O + Cholesta_5,7_dien_3beta_ol		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

2 H+ + 5alpha_Cholest_7_en_3beta_ol + 2 Ferrocytochrome_b5 + O2 -> Cholesta_5,7_dien_3beta_ol + 2 H2O + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

5alpha_Cholest_7_en_3beta_ol + 2 Ferrocytochrome_b5 + 2 H+ + O2 -> 2 H2O + Cholesta_5,7_dien_3beta_ol + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

5alpha_Cholest_7_en_3beta_ol + 2 H+ + 2 Ferrocytochrome_b5 + O2 -> Cholesta_5,7_dien_3beta_ol + 2 H2O + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

2 H+ + O2 + 2 Ferrocytochrome_b5 + 5alpha_Cholest_7_en_3beta_ol -> Cholesta_5,7_dien_3beta_ol + 2 H2O + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30
uniprot:Q39208 [NADH]

2 Ferrocytochrome_b5 + 2 H+ + O2 + 5alpha_Cholest_7_en_3beta_ol -> 2 H2O + Cholesta_5,7_dien_3beta_ol + 2 Ferricytochrome_b5		 (( (Vmax * S) ) / (Km + S)) buffer: 1.5 g/l Tween-80
PH: 7
Temperature: 30

Pathways

pathway id name
BioCyc:MOUSE_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:HUMAN_PWY66-341 cholesterol biosynthesis I
BioCyc:META_PWY-6075 ergosterol biosynthesis I
BioCyc:META_PWY66-341 cholesterol biosynthesis I
BioCyc:META_PWY66-4 cholesterol biosynthesis III (via desmosterol)
BioCyc:YEAST_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis
BioCyc:YEAST_PWY-6075 ergosterol biosynthesis
BioCyc:ARA_PWY-2541 plant sterol biosynthesis
BioCyc:MOUSE_PWY66-341 cholesterol biosynthesis I
BioCyc:HUMAN_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:META_PWY66-3 cholesterol biosynthesis II (via 24,25-dihydrolanosterol)
BioCyc:HUMAN_PWY66-4 cholesterol biosynthesis III (via desmosterol)
BioCyc:META_ERGOSTEROL-SYN-PWY superpathway of ergosterol biosynthesis I
BioCyc:META_PWY66-5 superpathway of cholesterol biosynthesis
BioCyc:MOUSE_PWY66-4 cholesterol biosynthesis III (via desmosterol)
PlantCyc:ARA_PWY-2541 phytosterol biosynthesis (plants)
PlantCyc:PLANT_PWY-2541 phytosterol biosynthesis (plants)
Reactome:R-HSA-9969901 Cholesterol biosynthesis from zymosterol (modified Kandutsch-Russell pathway)
Reactome:R-HSA-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-8957322 Metabolism of steroids
Reactome:R-SCE-8957322 Metabolism of steroids
Reactome:R-SPO-1430728 Metabolism
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-191273 Cholesterol biosynthesis
Reactome:R-HSA-2426168 Activation of gene expression by SREBF (SREBP)
Reactome:R-RNO-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-MMU-191273 Cholesterol biosynthesis
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-8957322 Metabolism of steroids
Reactome:R-SCE-556833 Metabolism of lipids
Reactome:R-SCE-191273 Cholesterol biosynthesis
Reactome:R-SPO-8957322 Metabolism of steroids
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-8957322 Metabolism of steroids
Reactome:R-HSA-1655829 Regulation of cholesterol biosynthesis by SREBP (SREBF)
Reactome:R-MMU-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-RNO-191273 Cholesterol biosynthesis
Reactome:R-SCE-6807047 Cholesterol biosynthesis via desmosterol (Bloch pathway)
Reactome:R-SPO-556833 Metabolism of lipids
Reactome:R-SPO-191273 Cholesterol biosynthesis
PathBank:SMP0120794 Mevalonic Aciduria
PathBank:SMP0120464 CHILD Syndrome
PathBank:SMP0120472 Desmosterolosis
PathBank:SMP0120512 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120573 Cholesteryl Ester Storage Disease
PathBank:SMP0121057 Bloch Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000023 Steroid Biosynthesis
PathBank:SMP0000387 CHILD Syndrome
PathBank:SMP0000112 Risedronate Action Pathway
PathBank:SMP0000508 Cholesteryl Ester Storage Disease
PathBank:SMP0002438 Cholesterol Biosynthesis and Metabolism CE(12:0)
PathBank:SMP0087326 Steroid Biosynthesis
PathBank:SMP0120685 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120769 Hypercholesterolemia
PathBank:SMP0120793 Hyper-IgD Syndrome
PathBank:SMP0120515 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120576 Wolman Disease
PathBank:SMP0000389 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0000319 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0000082 Simvastatin Action Pathway
PathBank:SMP0000111 Cerivastatin Action Pathway
PathBank:SMP0000131 Atorvastatin Action Pathway
PathBank:SMP0000511 Wolman Disease
PathBank:SMP0002381 Steroid Biosynthesis
PathBank:SMP0002436 Cholesterol biosynthesis and metabolism CE(10:0)
PathBank:SMP0002441 Cholesterol biosynthesis and metabolism CE(18:0)
PathBank:SMP0120684 CHILD Syndrome
PathBank:SMP0120692 Desmosterolosis
PathBank:SMP0120732 Lysosomal Acid Lipase Deficiency (Wolman Disease)
PathBank:SMP0120792 Cholesteryl Ester Storage Disease
PathBank:SMP0070041 Cholesterol Biosynthesis and Metabolism
PathBank:SMP0120465 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0120549 Hypercholesterolemia
PathBank:SMP0120574 Hyper-IgD Syndrome
PathBank:SMP0121060 Kandutsch-Russell Pathway (Cholesterol Biosynthesis)
PathBank:SMP0000386 Desmosterolosis
PathBank:SMP0000089 Pravastatin Action Pathway
PathBank:SMP0000095 Alendronate Action Pathway
PathBank:SMP0000099 Lovastatin Action Pathway
PathBank:SMP0000117 Pamidronate Action Pathway
PathBank:SMP0000509 Hyper-IgD Syndrome
PathBank:SMP0120735 Smith-Lemli-Opitz Syndrome (SLOS)
PathBank:SMP0120795 Wolman Disease
PathBank:SMP0120575 Mevalonic Aciduria
PathBank:SMP0121236 Steroid Biosynthesis
PathBank:SMP0000388 Chondrodysplasia Punctata II, X-Linked Dominant (CDPX2)
PathBank:SMP0000079 Ibandronate Action Pathway
PathBank:SMP0000092 Rosuvastatin Action Pathway
PathBank:SMP0000209 Hypercholesterolemia
PathBank:SMP0000107 Zoledronate Action Pathway
PathBank:SMP0000119 Fluvastatin Action Pathway
PathBank:SMP0000510 Mevalonic Aciduria
PathBank:SMP0002435 Cholesterol biosynthesis and metabolism CE(14:0)
PathBank:SMP0002440 Cholesterol Biosynthesis and Metabolism CE(16:0)
PathBank:SMP0063670 Steroid Biosynthesis