EC: 6.3.2.9

UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase (UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P14900 [Formate]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Formate]

UDP_N_acetylmuramoyl_L_alanine + D_Glutamate + ATP -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Formate]

UDP_N_acetylmuramoyl_L_alanine + ATP + D_Glutamate -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

UDP_N_acetylmuramoyl_L_alanine + D_Glutamate + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

UDP_N_acetylmuramoyl_L_alanine + ATP + D_Glutamate -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

D_Glutamate + UDP_N_acetylmuramoyl_L_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

D_Glutamate + UDP_N_acetylmuramoyl_L_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

D_Glutamate + UDP_N_acetylmuramoyl_L_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

ATP + UDP_N_acetylmuramoyl_L_alanine + D_Glutamate -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Formate]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Formate]

D_Glutamate + UDP_N_acetylmuramoyl_L_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Formate]

ATP + UDP_N_acetylmuramoyl_L_alanine + D_Glutamate -> ADP + Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

UDP_N_acetylmuramoyl_L_alanine + D_Glutamate + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

UDP_N_acetylmuramoyl_L_alanine + D_Glutamate + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Acetate]

UDP_N_acetylmuramoyl_L_alanine + D_Glutamate + ATP -> ADP + Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

D_Glutamate + UDP_N_acetylmuramoyl_L_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

ATP + D_Glutamate + UDP_N_acetylmuramoyl_L_alanine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37
uniprot:P14900 [Mg2+]

UDP_N_acetylmuramoyl_L_alanine + ATP + D_Glutamate -> ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamate + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 0.1 M Tris/HCl
PH: 9.4
Temperature: 37

Pathways

pathway id name
BioCyc:META_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:HPY_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-DAP-containing)
BioCyc:CAULO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-DAP-containing)
BioCyc:BSUB_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-6470 peptidoglycan biosynthesis V (β-lactam resistance)
BioCyc:GCF_000013425_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:META_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
BioCyc:ECOL199310_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:ANTHRA_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ANTHRA_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:AGRO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:AGRO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULONA1000_PWY-5746 peptidoglycan biosynthesis I
BioCyc:PDIF272563_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:PDIF272563_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:MTBH37RV_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBH37RV_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:HPY_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECOL199310_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CAULO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BSUB_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:GCF_000013425_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
PathBank:SMP0000792 D-Glutamine and D-Glutamate Metabolism
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0002074 Peptidoglycan Biosynthesis II