UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)

Sheng J, Huang L, Zhu X, Cai J, Xu Z. Reconstitution of the peptidoglycan cytoplasmic precursor biosynthetic pathway in cell-free system and rapid screening of antisense oligonucleotides for Mur enzymes. Applied Microbiology and Biotechnology. 2014 Jan 07;98(4):1785–94. doi: 10.1007/s00253-013-5467-8.; Murakami R, Muramatsu Y, Minami E, Masuda K, Sakaida Y, Endo S, Suzuki T, Ishida O, Takatsu T, Miyakoshi S, Inukai M, Isono F. A novel assay of bacterial peptidoglycan synthesis for natural product screening. J Antibiot (Tokyo). 2009 Mar;62(3):153–8. doi: 10.1038/ja.2009.4. PMID: 19229285.; Barreteau H, Kovac A, Boniface A, Sova M, Gobec S, Blanot D. Cytoplasmic steps of peptidoglycan biosynthesis. FEMS Microbiol Rev. 2008 Mar;32(2):168–207. doi: 10.1111/j.1574-6976.2008.00104.x. PMID: 18266853.; Delcour J, Ferain T, Deghorain M, Palumbo E, Hols P. The biosynthesis and functionality of the cell-wall of lactic acid bacteria. Antonie Van Leeuwenhoek. 1999 Nov;76(1-4):159–84. doi: 10.1023/a:1002089722581.

Metabolites

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

Magnesium cation

Formula: Mg (23.98505)

CAS ID: 22537-22-0

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

meso-2,6-Diaminoheptanedioate

Formula: C7H14N2O4 (190.0953524)

CAS ID: 922-54-3

Potassium cation

Formula: K (38.963708)

CAS ID: 24203-36-9

NADPH(4-)

Formula: C21H26N7O17P3 (741.0598016)

CAS ID: 53-57-6

ATP(4-)

Formula: C10H12N5O13P3 (502.9644492)

CAS ID:

UDP-N-acetyl-alpha-D-muramate(3-)

Formula: C20H28N3O19P2 (676.0792218)

CAS ID:

UDP-N-acetyl-alpha-D-glucosamine 3-phosphate(4-)

Formula: C17H24N3O20P3 (683.0166014)

CAS ID:

UDP(3-)

Formula: C9H11N2O12P2 (400.9787246)

CAS ID: 86527-70-0

ADP(3-)

Formula: C10H12N5O10P2 (424.0059412)

CAS ID:

NADP(3-)

Formula: C21H25N7O17P3 (740.051977)

CAS ID: 53-59-8

bromoacetate

Formula: C2H2BrO2 (136.9238152)

CAS ID: 68-10-0



Enzyme

EC Number name full name note
1.3.1.98 UDP-N-acetylmuramate dehydrogenase UDP-N-acetyl-alpha-D-muramate:NADP+ oxidoreductase
2.5.1.7 UDP-N-acetylglucosamine 1-carboxyvinyltransferase phosphoenolpyruvate:UDP-N-acetyl-D-glucosamine 1-carboxyvinyltransferase
5.1.1.3 glutamate racemase glutamate racemase
6.3.2.10 UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine:D-alanyl-D-alanine ligase (ADP-forming)
6.3.2.13 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate-2,6-diaminopimelate ligase UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:(L)-meso-2,6-diaminoheptanedioate gamma-ligase (ADP-forming)
6.3.2.4 D-Alanine-D-alanine ligase D-alanine:D-alanine ligase (ADP-forming)
6.3.2.8 UDP-N-acetylmuramate-L-alanine ligase UDP-N-acetylmuramate:L-alanine ligase (ADP-forming)
6.3.2.9 UDP-N-acetylmuramoyl-L-alanine-D-glutamate ligase UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (ADP-forming)


Proteins

Protein ID name full name
P07862 ddlB D-alanine--D-alanine ligase B
P08373 murB UDP-N-acetylenolpyruvoylglucosamine reductase
P0A6J8 ddlA D-alanine--D-alanine ligase A
P0A749 murA UDP-N-acetylglucosamine 1-carboxyvinyltransferase
P11880 murF UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
P14900 murD UDP-N-acetylmuramoylalanine--D-glutamate ligase
P17952 murC UDP-N-acetylmuramate--L-alanine ligase
P22188 murE UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
P22634 murI Glutamate racemase