uniprot:Q9A1B7 [glutamate racemase(Enzyme) wildtype]
D_Glutamate -> L_Glutamate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 2 mM DTT
PH: 7.5
Temperature: 30 |
uniprot:Q9A1B7 [glutamate racemase(Enzyme) wildtype]
L_Glutamate -> D_Glutamate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 2 mM DTT
PH: 7.5
Temperature: 30 |
uniprot:Q81LA8 [(4R,2S)-2-Cinnamyl-4-amino-5-hydroxypentanoic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [(2R,4S,E)-2-Amino-4-(3-phenylprop-2-enyl)pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [(2R,4S)-2-Amino-4-(2-naphthyl)methyl pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [(4R,2S)-2-Cinnamyl-4-amino-5-hydroxypentanoic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81UL8 [glutamate racemase(Enzyme) wildtype RacE1]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81UL8 [glutamate racemase(Enzyme) wildtype RacE1]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q81LA8 [glutamate racemase(Enzyme) mutant RacE2 V149A]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q81LA8 [glutamate racemase(Enzyme) mutant RacE2 V149A]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q81UL8 [(2R,4S,E)-2-Amino-4-(3-phenylprop-2-enyl)pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [(2R,4S,E)-2-Amino-4-(3-phenylprop-2-enyl)pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81UL8 [(2R,4S)-2-Amino-4-(2-naphthyl)methyl pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [(2R,4S)-2-Amino-4-(2-naphthyl)methyl pentanedioic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81UL8 [(4R,2S)-2-Cinnamyl-4-amino-5-hydroxypentanoic acid]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) / (1 + (Km / S))) ) * (1 + (I / Ki))) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [glutamate racemase(Enzyme) wildtype RacE2]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM CHES, 5 mM NAD+, 25.5 units/ml bovine L-glutamate adehydrogenase, 2.5 mM ADP, 0.65 mM INT, 2 units/ml diaphorase
PH: 9.2
Temperature: 25 |
uniprot:Q81LA8 [glutamate racemase(Enzyme) wildtype RacE2]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q81UL8 [glutamate racemase(Enzyme) wildtype RacE1]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q81LA8 [glutamate racemase(Enzyme) wildtype RacE2]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM potassium phosphate, 0.2 mM DTT
PH: 8.2
Temperature: 25 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant D10N]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant D10N]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant H186N]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant H186N]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant D36N]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant E152Q]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant D36N]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) mutant E152Q]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) wildtype]
L_Glutamate -> D_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) wildtype]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Trien-HCl, 5 mM NAD+, 37.5 units (S)-glutamate dehydrogenase (37.5 units), 2.5 mM ADP,0.65 mM INT, 2 units diaphorase
PH: 7.8
Temperature: 30 |
uniprot:Q03469 [glutamate racemase(Enzyme) wildtype]
D_Glutamate -> L_Glutamate |
(( (( (kcat * E) ) * S) ) / (Km + S)) |
|
buffer: 10 mM Potassium phosphate, 0.2 mM DTT
PH: 8
Temperature: 30 |
uniprot:P56068 [7-Isobutyl-5-methyl-2-(1-naphthylmethyl)-3-pyridin-4-yl-2H-pyrazolo[3,4-d]pyrimidine-4,6(5H,7H)-dione]
D_Glutamate -> L_Glutamate |
(( (Vmax * A) ) / (Km + (A * (( (( (1 + (A / Ki)) ) + (I / Kiapp)) ) + (( (I * A) ) / (Ki * Kiapp)))))) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
uniprot:P56068 [7-Isobutyl-5-methyl-2-(1-naphthylmethyl)-3-pyridin-4-yl-2H-pyrazolo[3,4-d]pyrimidine-4,6(5H,7H)-dione]
D_Glutamate -> L_Glutamate |
(( (Vmax * A) ) / (Km + (A * (( (( (1 + (A / Ki)) ) + (I / Kiapp)) ) + (( (I * A) ) / (Ki * Kiapp)))))) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
uniprot:P22634 [UDP-MurNAc-Ala]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
uniprot:Q836J0 [glutamate racemase(Enzyme) wildtype His6-tagged]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
uniprot:P56068 [glutamate racemase(Enzyme) mutant E151K]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
uniprot:P56068 [glutamate racemase(Enzyme) mutant A75T]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
uniprot:P56068 [glutamate racemase(Enzyme) wildtype]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
- [glutamate racemase(Enzyme) wildtype His6-tagged]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
- [glutamate racemase(Enzyme) wildtype His6-tagged]
L_Glutamate -> D_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 20 mM Ammonium acetate, 10 mM Magnesium acetate, 2 mM Tris(2-carboxyethyl)phosphine, 40 mM ATP, 400 μM 2-Amino-6-mercapto-7-methylpurine ribonucleoside,10 U/ml Purine nucleoside phosphorylase, 15 μg/ml recombinant E. faecalis MurD
PH: 8
Temperature: 36 |
uniprot:P56068 [7-Isobutyl-5-methyl-2-(1-naphthylmethyl)-3-pyridin-4-yl-2H-pyrazolo[3,4-d]pyrimidine-4,6(5H,7H)-dione]
D_Glutamate -> L_Glutamate |
(( (Vmax * A) ) / (Km + (A * (( (( (1 + (A / Ki)) ) + (I / Kiapp)) ) + (( (I * A) ) / (Ki * Kiapp)))))) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
uniprot:Q836J0 [glutamate racemase(Enzyme) wildtype His6-tagged]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
uniprot:P22634 [glutamate racemase(Enzyme) wildtype]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
- [glutamate racemase(Enzyme) wildtype His6-tagged]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
- [glutamate racemase(Enzyme) wildtype His6-tagged]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * A) ) / (Km + A)) |
|
buffer: 100 mM Tris-HCl, 10 mM beta-NAD, 5 mM DTT, 0.03% PEG-8000, 0.03 mg/ml BSA, 15 U/ml L-Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 36 |
uniprot:Q9X0Z7 [Pyridoxal phosphate]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Bicine, 5 mM NAD+, 0.4 mM Glutamate dehydrogenase from bovine liver
PH: 8
Temperature: 37 |
uniprot:Q9X0Z7 [Pyridoxal phosphate]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Bicine, 5 mM NAD+, 30 µM Glutamate dehydrogenase from Thermotoga maritima
PH: 8
Temperature: 70 |
uniprot:Q73GL9 [Pyridoxal phosphate]
D_Glutamate -> L_Glutamate |
(( (( (E * kcat) ) * S) ) / (Km + S)) |
|
buffer: 50 mM Tris-HCl, 5 mM NAD+, 0.4 U/ml Glutamate dehydrogenase from bovine liver
PH: 8.8
Temperature: 37 |