EC: 6.3.2.4

D-Alanine-D-alanine ligase (D-alanine:D-alanine ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q06893 [D-alanine---D-alanine ligase(Enzyme) wildtype VanB]

2 D_Alanine + ATP -> ADP + Phosphate + D_Alanyl_D_alanine		 (( (( (kcat * E) ) * (A ^ 2)) ) / (( (( (Km_A1 * Km_A2) ) + (Km_A2 * A)) ) + (A ^ 2))) buffer: 100 mM HEPES, 10 mM MgCl2, 10 mM KCl, 2.5 mM phosphoenolpyruvate, 0.1 mM NADH, 50 µg/ml lactate dehydrogenase, 0.14 mg/ml pyruvate kinase
PH: 7.8
Temperature: 36
uniprot:Q06893 [D-alanine---D-alanine ligase(Enzyme) wildtype VanB]

ATP + D_Alanine + D_2_Hydroxybutanoate -> D_Alanyl_D_2_hydroxybutanoate + ADP + Phosphate		 (( (kcat * E) ) / (1 + (( (Km_B / B) ) * (( (( (Km_A1 / A) ) + (A / Km_A2)) ) + 1)))) buffer: 100 mM HEPES, 10 mM KCl, 10 mM MgCl2, 2.5 mM phosphoenolpyruvate, 0.1 mM NADH, 50 µg/ml lactate dehydrogenase, 0.14 mg/ml pyruvate kinase
PH: 7.8
Temperature: 36
uniprot:Q06893 [D-alanine---D-alanine ligase(Enzyme) wildtype VanB]

D_Alanine + ATP + D_2_Hydroxyvalerate -> Phosphate + D_Alanyl_D_2_hydroxyvalerate + ADP		 (( (kcat * E) ) / (1 + (( (Km_B / B) ) * (( (( (Km_A1 / A) ) + (A / Km_A2)) ) + 1)))) buffer: 100 mM HEPES, 10 mM KCl, 10 mM MgCl2, 2.5 mM phosphoenolpyruvate, 0.1 mM NADH, 50 µg/ml lactate dehydrogenase, 0.14 mg/ml pyruvate kinase
PH: 7.8
Temperature: 36
uniprot:Q06893 [D-alanine---D-alanine ligase(Enzyme) wildtype VanB]

ATP + D_Lactate + D_Alanine -> D_Alanyl_D_lactate + Phosphate + ADP		 (( (kcat * E) ) / (1 + (( (Km_B / B) ) * (( (( (Km_A1 / A) ) + (A / Km_A2)) ) + 1)))) buffer: 100 mM HEPES, 10 mM KCl, 10 mM MgCl2, 2.5 mM phosphoenolpyruvate, 0.1 mM NADH, 50 µg/ml lactate dehydrogenase, 0.14 mg/ml pyruvate kinase
PH: 7.8
Temperature: 36
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + ATP + D_Alanyl_D_alanine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + ADP + Phosphate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-2-Aminobutyryl-D-alanine]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + ATP + D_Alanyl_D_alanine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Alanyl-D-2-aminobutyrate]

ATP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_alanine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + ADP + Phosphate		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_alanine + ATP -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + ADP		 (( (( (Vmax * (1 / (1 + (Ka / A)))) ) * (1 / (1 + (Kb / B)))) ) * (1 / (1 + (Kc / C)))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Alanyl-D-alanine]

ATP + D_Alanyl_D_serine + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_serine + Phosphate + ADP		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

D_Alanyl_D_serine + ATP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_serine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Alanyl-D-alanine]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + ATP + D_Alanyl_D_threonine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_threonine + Phosphate + ADP		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Alanyl-D-alanine]

ATP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_2_aminobutyrate -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_2_aminobutyrate + ADP + Phosphate		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_threonine + ATP -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_threonine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_2_aminobutyrate + ATP -> ADP + Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_2_aminobutyrate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-2-Aminobutyryl-D-2-aminobutyrate]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_alanine + ATP -> ADP + Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Seryl-D-alanine]

D_Alanyl_D_alanine + ATP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + Phosphate + ADP		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Norvalinyl-D-alanine]

ATP + D_Alanyl_D_alanine + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine -> Phosphate + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + ADP		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Valinyl-D-alanine]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + D_Alanyl_D_alanine + ATP -> UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine + Phosphate + ADP		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-Alanyl-D-valine]

UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysine + ATP + D_Alanyl_D_alanine -> Phosphate + ADP + UDP_N_acetylmuramoyl_L_alanyl_D_glutamyl_L_lysyl_D_alanyl_D_alanine		 (Vmax / (1 + (( (Ka / A) ) * (1 + (B / Kb))))) buffer: 100 mM Tris-HCl, 10 mM MgCl2
PH: 7.6
Temperature: 37
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

2 D_Alanine + ATP -> ADP + D_Alanyl_D_alanine + Phosphate		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM HEPES, 20 mM MgCl2, 10 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.5
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> D_Alanyl_D_alanine + ADP + Phosphate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ADP + Phosphate + D_Alanyl_D_alanine -> ATP + 2 D_Alanine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 0.2 mM NADP, 100 mM KCl, 2.0 mM Glucose, 8.33 units/ml Hexokinase, 1.66 units/ml Glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

D_Alanyl_D_alanine + ADP + Phosphate -> ATP + 2 D_Alanine		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 0.2 mM NADP, 100 mM KCl, 2.0 mM Glucose, 8.33 units/ml Hexokinase, 1.66 units/ml Glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> ADP + D_Alanyl_D_alanine + Phosphate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

2 D_Alanine + ATP -> ADP + D_Alanyl_D_alanine + Phosphate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ADP + D_Alanyl_D_alanine + Phosphate -> 2 D_Alanine + ATP		 (( (( (Vmax * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmB * A)) ) + (KmA * B)) ) + (A * B))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 0.2 mM NADP, 100 mM KCl, 2.0 mM Glucose, 8.33 units/ml Hexokinase, 1.66 units/ml Glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> ADP + Phosphate + D_Alanyl_D_alanine		 (( (Vmax * A) ) / (Km + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> Phosphate + ADP + D_Alanyl_D_alanine		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

D_Alanyl_D_alanine + Phosphate + ADP -> ATP + 2 D_Alanine		 (( (Vmax * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 0.2 mM NADP, 100 mM KCl, 2.0 mM Glucose, 8.33 units/ml Hexokinase, 1.66 units/ml Glucose 6-phosphate dehydrogenase
PH: 7.5
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> D_Alanyl_D_alanine + Phosphate + ADP		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> Phosphate + ADP + D_Alanyl_D_alanine		 (( (Vmax * A) ) / (Km + (A * (1 + (I / Kii))))) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25
- [D-alanine---D-alanine ligase(Enzyme) wildtype]

ATP + 2 D_Alanine -> D_Alanyl_D_alanine + ADP + Phosphate		 (( (Vmax * A) ) / (( (Km * (1 + (I / Kis))) ) + A)) buffer: 100 mM HEPES, 7.5 mM MgCl2, 100 mM KCl, 1.0 mM Phosphoenolpyruvate, 0.2 mM NADH, 11 µg/ml Pyruvate kinase, 11 µg/ml Lactate dehydrogenase
PH: 7.6
Temperature: 25

Pathways

pathway id name
BioCyc:META_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:HPY_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-DAP-containing)
BioCyc:BSUB_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-6470 peptidoglycan biosynthesis V (β-lactam resistance)
BioCyc:GCF_000013425_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:META_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
BioCyc:VCHO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:VCHO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBCDC1551_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:ANTHRA_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ANTHRA_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:AGRO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECO_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBCDC1551_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:AGRO_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:SYNEL_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BTHE_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:BTHE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:CORYNE_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:META_PWY-6386 UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing)
BioCyc:MTBH37RV_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:MTBH37RV_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:HPY_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL199310_PWY-6385 peptidoglycan biosynthesis III (mycobacteria)
BioCyc:ECOL199310_PEPTIDOGLYCANSYN-PWY peptidoglycan biosynthesis I (meso-diaminopimelate containing)
BioCyc:BSUB_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:ECOL316407_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:BTHE_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-DAP-containing)
BioCyc:CORYNE_PWY-6387 UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing)
BioCyc:GCF_000013425_PWY-5265 peptidoglycan biosynthesis II (staphylococci)
PathBank:SMP0000791 D-Alanine Metabolism
PathBank:SMP0000810 L-Alanine Metabolism
PathBank:SMP0000924 Peptidoglycan Biosynthesis I
PathBank:SMP0002074 Peptidoglycan Biosynthesis II