EC: 6.3.2.17

tetrahydrofolate synthase (tetrahydropteroyl-gamma-polyglutamate:L-glutamate gamma-ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P15925 [tetrahydrofolate synthase(Enzyme) wildtype]

n_1_n_1_n_1_ATP + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_diglutamate + n_1_n_1_n_1_L_Glutamate -> n_1_n_1_n_1_ADP + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_triglutamate + n_1_n_1_n_1_Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 50 mM glycine, 10 mM MgCl2, 200 mM KCl, 5 mM DTT, 10 mM beta-mercaptoethanol, 100 µg/ml BSA
PH: 9.75
Temperature: 37
uniprot:P15925 [tetrahydrofolate synthase(Enzyme) wildtype]

n_1_n_1_n_1_L_Glutamate + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_diglutamate + n_1_n_1_n_1_ATP -> n_1_n_1_n_1_ADP + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_triglutamate + n_1_n_1_n_1_Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 50 mM glycine, 10 mM MgCl2, 200 mM KCl, 5 mM DTT, 10 mM beta-mercaptoethanol, 100 µg/ml BSA
PH: 9.75
Temperature: 37
uniprot:P15925 [tetrahydrofolate synthase(Enzyme) wildtype]

n_1_n_1_n_1_L_Glutamate + n_1_n_1_n_1_ATP + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_diglutamate -> n_1_n_1_n_1_ADP + n_1_n_1_n_1_Phosphate + (6R)_5,10_Methylene_5,6,7,8_tetrahydropteroyl_triglutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris, 50 mM glycine, 10 mM MgCl2, 200 mM KCl, 5 mM DTT, 10 mM beta-mercaptoethanol, 100 µg/ml BSA
PH: 9.75
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-2161 folate polyglutamylation
BioCyc:META_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:ECO_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-2161 folate polyglutamylation
BioCyc:ARA_PWY-2161B-PMN folate polyglutamylation II
BioCyc:ARA_PWY-2161 folate polyglutamylation
BioCyc:MTBH37RV_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECOL316407_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:META_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:META_PWY-2161 folate polyglutamylation
BioCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
BioCyc:MTBH37RV_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:MTBH37RV_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:MOUSE_PWY-2161 folate polyglutamylation
BioCyc:BSUB_PWY-2161 folate polyglutamylation
BioCyc:ECOL316407_PWY-6612 superpathway of tetrahydrofolate biosynthesis
BioCyc:ECOL316407_PWY-2161 folate polyglutamylation
BioCyc:META_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:ECO_PWY-6614 tetrahydrofolate biosynthesis
BioCyc:YEAST_PWY-2161 folate polyglutamylation
BioCyc:META_FOLSYN-PWY superpathway of tetrahydrofolate biosynthesis and salvage
BioCyc:ECO_ALL-CHORISMATE-PWY superpathway of chorismate metabolism
BioCyc:MTBH37RV_PWY-2161 folate polyglutamylation
BioCyc:ECOL316407_1CMET2-PWY formylTHF biosynthesis
PathBank:SMP0000925 Folate Biosynthesis
PlantCyc:ARA_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:ARA_PWY-2161B-PMN folate polyglutamylation II
PlantCyc:ARA_PWY-2161 folate polyglutamylation
PlantCyc:PLANT_PWY-2161B-PMN folate polyglutamylation II
PlantCyc:PLANT_PWY-3742 tetrahydrofolate biosynthesis II
PlantCyc:PLANT_PWY-2161 folate polyglutamylation
Reactome:R-DDI-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-1430728 Metabolism
Reactome:R-CEL-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-196757 Metabolism of folate and pterines
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196757 Metabolism of folate and pterines
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196757 Metabolism of folate and pterines
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-CEL-196757 Metabolism of folate and pterines
Reactome:R-DDI-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-196757 Metabolism of folate and pterines
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
PathBank:SMP0000432 Methotrexate Action Pathway
PathBank:SMP0000543 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0087196 Folate Metabolism
PathBank:SMP0120656 Folate Malabsorption, Hereditary
PathBank:SMP0120522 Methylenetetrahydrofolate Reductase Deficiency (MTHFRD)
PathBank:SMP0000053 Folate Metabolism
PathBank:SMP0000724 Folate Malabsorption, Hereditary
PathBank:SMP0000801 Folate Metabolism
PathBank:SMP0012063 Folate Polyglutamylation