EC: 6.3.1.2

glutamine synthetase (L-glutamate:ammonia ligase (ADP-forming))

enzyme lambda metabolic reaction experiment
uniprot:P14654 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;2]

L_Glutamate + ATP + NH3 -> ADP + Phosphate + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:P14654 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;2]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:P14656 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;1]

ATP + L_Glutamate + NH3 -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:P14656 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;1]

L_Glutamate + NH3 + ATP -> L_Glutamine + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:P14656 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;1]

L_Glutamate + NH3 + ATP -> L_Glutamine + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:P14654 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme OsGLN1;2]

L_Glutamate + NH3 + ATP -> Phosphate + L_Glutamine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-mercaptoethanol
PH: 7.8
Temperature: 30
- [Magnesium chloride]

ATP + L_Glutamate + NH3 -> L_Glutamine + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

NH3 + L_Glutamate + ATP -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

NH3 + L_Glutamate + ATP -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

ATP + NH3 + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

ATP + L_Glutamate + NH3 -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

NH3 + ATP + L_Glutamate -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [MnCl2]

NH3 + ATP + L_Glutamate -> L_Glutamine + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

L_Glutamate + NH3 + ATP -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [Magnesium chloride]

Hydroxylamine + L_Glutamate + ATP -> L_gamma_Glutamyl_hydroxamate + ADP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

ATP + L_Glutamate + Hydroxylamine -> Phosphate + L_gamma_Glutamyl_hydroxamate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

Hydroxylamine + ATP + L_Glutamate -> L_gamma_Glutamyl_hydroxamate + ADP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

Hydroxylamine + L_Glutamate + ATP -> L_gamma_Glutamyl_hydroxamate + ADP + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

ATP + Hydroxylamine + L_Glutamate -> ADP + Phosphate + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [Magnesium chloride]

L_Glutamate + Hydroxylamine + ATP -> L_gamma_Glutamyl_hydroxamate + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 7.5
Temperature: 70
- [MnCl2]

Hydroxylamine + L_Glutamate + ATP -> Phosphate + ADP + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + Hydroxylamine + L_Glutamate -> Phosphate + L_gamma_Glutamyl_hydroxamate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + L_Glutamate + Hydroxylamine -> L_gamma_Glutamyl_hydroxamate + Phosphate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + Hydroxylamine + L_Glutamate -> ADP + L_gamma_Glutamyl_hydroxamate + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + Hydroxylamine + L_Glutamate -> Phosphate + ADP + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + Hydroxylamine + L_Glutamate -> Phosphate + ADP + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + L_Glutamate + NH3 -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

ATP + L_Glutamate + NH3 -> Phosphate + L_Glutamine + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
- [MnCl2]

L_Glutamate + ATP + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole-acetate
PH: 6.3
Temperature: 70
uniprot:P12425 [glutamate---ammonia ligase(Enzyme) wildtype]

L_Glutamate + ATP + NH3 -> Phosphate + L_Glutamine + ADP		 (( (Vmax * (S ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:P12425 [glutamate---ammonia ligase(Enzyme) mutant S186F]

NH3 + L_Glutamate + ATP -> ADP + Phosphate + L_Glutamine		 (( (Vmax * (S ^ n)) ) / (( (S ^ n) ) + (S_half ^ n))) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:P12425 [glutamate---ammonia ligase(Enzyme) wildtype]

NH3 + ATP + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:P12425 [glutamate---ammonia ligase(Enzyme) mutant S186F]

L_Glutamate + NH3 + ATP -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

NH3 + ATP + L_Glutamate -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S1) ) / (Km + S1)) buffer: *
PH: 7
Temperature: 36
uniprot:P12425 [ADP]

L_Glutamine + Hydroxylamine -> NH3 + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium arsenate, 25 mM imidazole, 25 mM Tris, 0.33 mM MnCl2 and 0.4 M K2SO4
PH: 7.5
Temperature: 36
uniprot:P12425 [ADP]

L_Glutamine + Hydroxylamine -> NH3 + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium arsenate, 25 mM imidazole, 25 mM Tris, 0.33 mM MnCl2 and 0.4 M K2SO4
PH: 7.5
Temperature: 36
uniprot:P12425 [ADP]

L_Glutamine + Hydroxylamine -> L_gamma_Glutamyl_hydroxamate + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium arsenate, 25 mM imidazole, 25 mM Tris, 0.33 mM MnCl2 and 0.4 M K2SO4
PH: 7.5
Temperature: 36
uniprot:P12425 [ADP]

Hydroxylamine + L_Glutamine -> NH3 + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM sodium arsenate, 25 mM imidazole, 25 mM Tris, 0.33 mM MnCl2 and 0.4 M K2SO4
PH: 7.5
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

ATP + NH3 + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S1) ) / (Km + S1)) buffer: *
PH: 7
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

L_Glutamate + ATP + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S2) ) / (Km + S2)) buffer: *
PH: 7
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

NH3 + ATP + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * S2) ) / (Km + S2)) buffer: *
PH: 7
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

L_Glutamate + ATP + Hydroxylamine -> ADP + L_gamma_Glutamyl_hydroxamate + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: *
PH: 7
Temperature: 36
uniprot:O04998 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GS1a, his-tagged]

Hydroxylamine + L_Glutamate + ATP -> ADP + Phosphate + L_gamma_Glutamyl_hydroxamate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: *
PH: 7
Temperature: 36
uniprot:P12425 [L-Methionine sulfoximine]

L_Glutamate + NH3 + ATP -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:P12425 [L-Methionine sulfoximine]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 0.05 M imidazole, 40 mM hydroxylamine, 100 mM MgCl2
PH: 7
Temperature: 36
uniprot:P04771 [Mg2+]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

L_Glutamate + NH3 + ATP -> ADP + Phosphate + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + ATP + L_Glutamate -> L_Glutamine + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + ATP + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:Q8LCE1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;2]

ATP + L_Glutamate + NH3 -> Phosphate + L_Glutamine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q8LCE1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;2]

L_Glutamate + NH3 + ATP -> L_Glutamine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q8LCE1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;2]

ATP + L_Glutamate + NH3 -> ADP + L_Glutamine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q56WN1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;1]

ATP + NH3 + L_Glutamate -> L_Glutamine + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q56WN1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;1]

NH3 + ATP + L_Glutamate -> L_Glutamine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q56WN1 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;1]

NH3 + ATP + L_Glutamate -> Phosphate + L_Glutamine + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q4ZH57 [Mg2+]

ATP + NH3 + L_Glutamate -> L_Glutamine + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 94 mM Imidazole
PH: 6.2
Temperature: 37
uniprot:Q4ZH57 [Mg2+]

NH3 + ATP + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 94 mM Imidazole
PH: 6.2
Temperature: 37
uniprot:Q4ZH57 [Mg2+]

L_Glutamate + ATP + NH3 -> L_Glutamine + Phosphate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 94 mM Imidazole
PH: 6.2
Temperature: 37
uniprot:Q4ZH57 [Mg2+]

L_Glutamate + NH3 + ATP -> Phosphate + L_Glutamine + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 94 mM Imidazole
PH: 6.2
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + L_Glutamate + ATP -> L_Glutamine + ADP + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

ATP + NH3 + L_Glutamate -> Phosphate + ADP + L_Glutamine		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + L_Glutamate + ATP -> L_Glutamine + Phosphate + ADP		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + ATP + L_Glutamate -> Phosphate + ADP + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

NH3 + L_Glutamate + ATP -> ADP + Phosphate + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

ATP + L_Glutamate + NH3 -> L_Glutamine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P04771 [Mg2+]

ATP + NH3 + L_Glutamate -> ADP + Phosphate + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
- [glutamate---ammonia ligase(Enzyme) wildtype GS1]

ATP + L_Glutamate + NH3 -> L_Glutamine + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
- [glutamate---ammonia ligase(Enzyme) wildtype GS1]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
- [glutamate---ammonia ligase(Enzyme) wildtype GS1]

NH3 + L_Glutamate + ATP -> L_Glutamine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;4]

NH3 + L_Glutamate + ATP -> ADP + L_Glutamine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;3]

L_Glutamate + NH3 + ATP -> ADP + Phosphate + L_Glutamine		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;4]

NH3 + L_Glutamate + ATP -> L_Glutamine + ADP + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;3]

ATP + NH3 + L_Glutamate -> ADP + L_Glutamine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;4]

ATP + NH3 + L_Glutamate -> L_Glutamine + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) wildtype isoenzyme GLN1;3]

ATP + L_Glutamate + NH3 -> L_Glutamine + Phosphate + ADP		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;3]

ATP + NH3 + L_Glutamate -> L_Glutamine + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_A174S]

ATP + L_Glutamate + NH3 -> L_Glutamine + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_K49Q]

ATP + L_Glutamate + NH3 -> L_Glutamine + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;3]

NH3 + ATP + L_Glutamate -> Phosphate + L_Glutamine + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_K49Q]

L_Glutamate + NH3 + ATP -> L_Glutamine + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_K49Q_A174S]

ATP + L_Glutamate + NH3 -> L_Glutamine + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;4]

NH3 + ATP + L_Glutamate -> ADP + Phosphate + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;4_Q49K]

NH3 + L_Glutamate + ATP -> L_Glutamine + ADP + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_A174S]

L_Glutamate + ATP + NH3 -> L_Glutamine + Phosphate + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9LVI8 [glutamate---ammonia ligase(Enzyme) mutant GLN1;3_K49Q_A174S]

NH3 + ATP + L_Glutamate -> Phosphate + ADP + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;4_S174A]

ATP + NH3 + L_Glutamate -> Phosphate + L_Glutamine + ADP		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;4_Q49K_S174A]

L_Glutamate + ATP + NH3 -> Phosphate + ADP + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) mutant GLN1;4_Q49K]

L_Glutamate + NH3 + ATP -> ADP + L_Glutamine + Phosphate		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) wildtype GLN1;4]

ATP + NH3 + L_Glutamate -> ADP + Phosphate + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) mutant GLN1;4_S174A]

ATP + L_Glutamate + NH3 -> Phosphate + ADP + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30
uniprot:Q9FMD9 [glutamate---ammonia ligase(Enzyme) mutant GLN1;4_Q49K_S174A]

NH3 + L_Glutamate + ATP -> ADP + Phosphate + L_Glutamine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 100 mM Tricine-HCl, 20 mM MgSO4, 8 mM 2-Mercaptoethanol
PH: 7.8
Temperature: 30

Pathways

pathway id name
BioCyc:META_PWY-6964 ammonia assimilation cycle II
BioCyc:META_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:META_PWY-5505 L-glutamate and L-glutamine biosynthesis
BioCyc:META_PWY-6549 L-glutamine biosynthesis III
BioCyc:ECO_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:YEAST_PWY-6963 ammonia assimilation cycle I
BioCyc:MTBCDC1551_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ECOO157_GLNSYN-PWY glutamine biosynthesis I
BioCyc:ECOL199310_GLNSYN-PWY glutamine biosynthesis I
BioCyc:BSUB_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:HUMAN_GLUDEG-I-PWY GABA shunt
BioCyc:HUMAN_PWY0-1305 glutamate dependent acid resistance
BioCyc:META_PWY-6963 ammonia assimilation cycle I
BioCyc:META_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ARA_PWY-6549 L-glutamine biosynthesis III
BioCyc:ARA_PWY-7060 ornithine-citrulline shuttle
BioCyc:VCHO_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ECOL316407_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:HUMAN_GLNSYN-PWY glutamine biosynthesis
BioCyc:META_PWY-3282 superpathway of ammonia assimilation (plants)
BioCyc:META_PWY-5675 nitrate reduction V (assimilatory)
BioCyc:ECO_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:YEAST_GLNSYN-PWY glutamine biosynthesis
BioCyc:ARA_PWY-6963 ammonia assimilation cycle I
BioCyc:MTBH37RV_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:ECOO157_AMMASSIM-PWY ammonia assimilation cycle III
BioCyc:ECOL316407_GLNSYN-PWY glutamine biosynthesis I
BioCyc:META_PWY-381 nitrate reduction II (assimilatory)
BioCyc:ARA_PWY-381 nitrate reduction II (assimilatory)
BioCyc:ARA_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:ARA_PWY-7061 glutamate-glutamine shuttle
BioCyc:ARA_PWY-6964 ammonia assimilation cycle II
BioCyc:VCHO_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:MTBCDC1551_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:HPY_GLNSYN-PWY L-glutamine biosynthesis I
BioCyc:MOUSE_GLUTAMINE-SYN2-PWY glutamine biosynthesis II
BioCyc:BSUB_GLNSYN-PWY L-glutamine biosynthesis I
PathBank:SMP0000778 Nitrogen Metabolism
PathBank:SMP0000792 D-Glutamine and D-Glutamate Metabolism
PathBank:SMP0000811 L-Glutamate Metabolism
Plant Reactome:R-OSA-1119443 Glutamate synthase cycle
Plant Reactome:R-OSA-2744345 Metabolism and regulation
Plant Reactome:R-OSA-2744343 Amino acid metabolism
Plant Reactome:R-OSA-1119293 Glutamine biosynthesis I
Plant Reactome:R-OSA-5655122 Amino acid biosynthesis
Plant Reactome:R-OSA-5368291 Inorganic nutrients metabolism
PlantCyc:ARA_PWY-6963 ammonia assimilation cycle I
PlantCyc:ARA_GLNSYN-PWY L-glutamine biosynthesis I
PlantCyc:PLANT_PWY-381 nitrate reduction II (assimilatory)
PlantCyc:PLANT_PWY-7061 glutamate-glutamine shuttle
PlantCyc:ARA_PWY-6964 ammonia assimilation cycle II
PlantCyc:PLANT_GLNSYN-PWY L-glutamine biosynthesis I
PlantCyc:ARA_PWY-7060 ornithine-citrulline shuttle
PlantCyc:PLANT_PWY-3282 superpathway of ammonia assimilation (plants)
PlantCyc:ARA_PWY-3282 superpathway of ammonia assimilation (plants)
PlantCyc:ARA_PWY-7061 glutamate-glutamine shuttle
PlantCyc:PLANT_PWY-6549 L-glutamine biosynthesis III
PlantCyc:ARA_PWY-381 nitrate reduction II (assimilatory)
PlantCyc:PLANT_PWY-6963 ammonia assimilation cycle I
PlantCyc:PLANT_PWY-7060 ornithine-citrulline shuttle
PlantCyc:PLANT_PWY-6964 ammonia assimilation cycle II
PlantCyc:ARA_PWY-6549 L-glutamine biosynthesis III
Reactome:R-RNO-8964539 Glutamate and glutamine metabolism
Reactome:R-SCE-8964539 Glutamate and glutamine metabolism
Reactome:R-SPO-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-HSA-8964539 Glutamate and glutamine metabolism
Reactome:R-BTA-8964539 Glutamate and glutamine metabolism
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-71291 Amino acid and derivative metabolism
Reactome:R-BTA-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-HSA-112315 Transmission across Chemical Synapses
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
Reactome:R-RNO-112316 Neuronal System
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-SCE-112315 Transmission across Chemical Synapses
Reactome:R-SPO-112316 Neuronal System
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-112316 Neuronal System
Reactome:R-HSA-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
Reactome:R-MMU-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-RNO-112315 Transmission across Chemical Synapses
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-112316 Neuronal System
Reactome:R-SPO-71291 Amino acid and derivative metabolism
Reactome:R-BTA-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-HSA-112316 Neuronal System
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-MMU-112315 Transmission across Chemical Synapses
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-RNO-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-SCE-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-SPO-112315 Transmission across Chemical Synapses
Reactome:R-SPO-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-BTA-112315 Transmission across Chemical Synapses
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-112313 Neurotransmitter uptake and metabolism In glial cells
Reactome:R-MMU-112316 Neuronal System
Reactome:R-RNO-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
Reactome:R-SCE-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
Reactome:R-SPO-210455 Astrocytic Glutamate-Glutamine Uptake And Metabolism
WikiPathways:WP5298 Dravet syndrome: Scn1a-A1783V point mutation model
WikiPathways:WP5083 Neuroinflammation and glutamatergic signaling
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0063579 Ammonia Recycling
PathBank:SMP0002403 Nitrogen Metabolism
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0002426 Glutamine Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency