EC: 4.4.1.13

cysteine-S-conjugate beta-lyase (L-cysteine-S-conjugate thiol-lyase (deaminating; pyruvate-forming))

enzyme lambda metabolic reaction experiment
uniprot:O15382 [cysteine-S-conjugate beta-lyase(Enzyme) wildtype isoenzym BCAT(m)]

3_Chloro_L_alanine + H2O -> Pyruvate + NH3 + Cl_		 (( (V * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 37.5 µg/ml lactate dehydrogenase, 0.05 mM NADH
PH: 7.4
Temperature: 23
uniprot:O15382 [cysteine-S-conjugate beta-lyase(Enzyme) wildtype isoenzym BCAT(m)]

H2O + S_(1,1,2,2_Tetrafluoroethyl)_L_cysteine -> Pyruvate + NH3 + 1,1,2,2_Tetrafluoroethanethiol		 (( (V * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 37.5 µg/ml lactate dehydrogenase, 0.05 mM NADH
PH: 7.4
Temperature: 23
uniprot:Q73GL9 [Pyridoxal phosphate]

L_Cystathionine + H2O -> L_Homocysteine + NH3 + Pyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.8 mM DTNB
PH: 8.8
Temperature: 37
uniprot:Q4FME3 [Pyridoxal phosphate]

L_Cystathionine + H2O -> Pyruvate + NH3 + L_Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.8 mM DTNB
PH: 8.8
Temperature: 37
uniprot:Q9X0Z7 [Pyridoxal phosphate]

H2O + L_Cystathionine -> NH3 + Pyruvate + L_Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Bicine, 0.8 mM DTNB
PH: 8
Temperature: 37
uniprot:Q9X0Z7 [Pyridoxal phosphate]

L_Cystathionine + H2O -> NH3 + L_Homocysteine + Pyruvate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Bicine, 0.8 mM DTNB
PH: 8
Temperature: 70
uniprot:Q9X0Z7 [Pyridoxal phosphate]

H2O + L_Cystathionine -> NH3 + Pyruvate + L_Homocysteine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 0.8 mM DTNB
PH: 8.8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-4361 S-methyl-5-thio-α-D-ribose 1-phosphate degradation
BioCyc:META_PWY-6842 glutathione-mediated detoxification II
BioCyc:META_PWY-7527 L-methionine salvage cycle III
BioCyc:META_PWY-801 homocysteine and cysteine interconversion
BioCyc:YEAST_PWY-6842-1 glutathione-mediated detoxification II
BioCyc:ARA_PWY-801 L-homocysteine and L-cysteine interconversion
BioCyc:ECOL316407_P4-PWY superpathway of lysine, threonine and methionine biosynthesis I
BioCyc:ECOL316407_METSYN-PWY homoserine and methionine biosynthesis
BioCyc:ECOL316407_PWY0-781 aspartate superpathway
BioCyc:META_PWY-6293 superpathway of L-cysteine biosynthesis (fungi)
BioCyc:META_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ARA_PWY-702 L-methionine biosynthesis II
BioCyc:ECOL316407_LCYSDEG-PWY L-cysteine degradation II
BioCyc:ECOL316407_HOMOSER-METSYN-PWY methionine biosynthesis I
BioCyc:ECOL316407_MET-SAM-PWY superpathway of S-adenosyl-L-methionine biosynthesis
BioCyc:HUMAN_PWY-7527 methionine salvage cycle III
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:META_PWY-4361 S-methyl-5-thio-α-D-ribose 1-phosphate degradation I
BioCyc:YEAST_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
BioCyc:ARA_PWY-6936 seleno-amino acid biosynthesis
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:YEAST_PWY-801 homocysteine and cysteine interconversion
BioCyc:YEAST_PWY-6293 cysteine biosynthesis IV (fungi)
PathBank:SMP0000834 Methionine Biosynthesis
PathBank:SMP0000939 Sulfur Metabolism
PathBank:SMP0000940 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0000941 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0000942 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000943 Sulfur Metabolism (Isethionate)
PathBank:SMP0000944 Sulfur Metabolism (Methanesulfonate)
PlantCyc:ARA_PWY-702 L-methionine biosynthesis II (plants)
PlantCyc:ARA_PWY-6842 glutathione-mediated detoxification II
PlantCyc:ARA_PWY-6395 superpathway of seleno-compound metabolism
PlantCyc:ARA_PWY-724 superpathway of L-lysine, L-threonine and L-methionine biosynthesis II
PlantCyc:ARA_PWY-801 homocysteine and cysteine interconversion
PlantCyc:PLANT_PWY-6842 glutathione-mediated detoxification II
PlantCyc:ARA_PWY-6936 seleno-amino acid biosynthesis (plants)
PlantCyc:PLANT_PWY-801 homocysteine and cysteine interconversion
Reactome:R-RNO-8964539 Glutamate and glutamine metabolism
Reactome:R-HSA-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-RNO-8964208 Phenylalanine metabolism
Reactome:R-DDI-8964539 Glutamate and glutamine metabolism
Reactome:R-MMU-8964539 Glutamate and glutamine metabolism
Reactome:R-RNO-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-DDI-8964208 Phenylalanine metabolism
Reactome:R-MMU-8964208 Phenylalanine metabolism
Reactome:R-HSA-8964539 Glutamate and glutamine metabolism
Reactome:R-DDI-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-MMU-8963691 Phenylalanine and tyrosine metabolism
Reactome:R-HSA-8964208 Phenylalanine metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-DDI-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-MMU-71240 Tryptophan catabolism
Reactome:R-RNO-71240 Tryptophan catabolism
Reactome:R-DDI-71240 Tryptophan catabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
WikiPathways:WP465 Tryptophan metabolism
PathBank:SMP0002309 Cysteine Metabolism
PathBank:SMP0002371 Selenocompound Metabolism