EC: 4.2.1.3

aconitate hydratase (citrate(isocitrate) hydro-lyase (cis-aconitate-forming))

enzyme lambda metabolic reaction experiment
uniprot:P36683 [aconitate hydratase(Enzyme) wildtype]

H2O + cis_Aconitate -> Citrate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P36683 [aconitate hydratase(Enzyme) wildtype]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P25516 [aconitate hydratase(Enzyme) wildtype]

Isocitrate -> H2O + cis_Aconitate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P25516 [aconitate hydratase(Enzyme) wildtype]

H2O + cis_Aconitate -> Citrate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P36683 [aconitate hydratase(Enzyme) wildtype]

Citrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P25516 [aconitate hydratase(Enzyme) wildtype]

Citrate -> H2O + cis_Aconitate		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P36683 [aconitate hydratase(Enzyme) wildtype]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
uniprot:P25516 [aconitate hydratase(Enzyme) wildtype]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 20 mM Tris/HCl
PH: 7.4
Temperature: 20
- [aconitate hydratase(Enzyme) wildtype]

Citrate -> Isocitrate		 (( (Vmax * S) ) / (Km + S)) buffer:
PH: 7
Temperature: 37
- [aconitate hydratase(Enzyme) wildtype]

Citrate -> Isocitrate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM KH2PO4-KOH
PH: 7.5
Temperature: 30
uniprot:Q9NJQ8 [[4Fe-4S] cluster]

Citrate -> H2O + cis_Aconitate		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.5
Temperature: 25
uniprot:Q9NJQ8 [[4Fe-4S] cluster]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.5
Temperature: 25
uniprot:Q9NJQ8 [[4Fe-4S] cluster]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 90 mM Tris-HCl
PH: 7.5
Temperature: 25
- [aconitate hydratase(Enzyme) wildtype]

Isocitrate -> Citrate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM KH2PO4-KOH
PH: 7.5
Temperature: 30
- [aconitate hydratase(Enzyme) wildtype late aconitase (12h culture age)]

Citrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate
PH: 7.4
Temperature: 36
- [aconitate hydratase(Enzyme) wildtype early aconitase (5h culture age)]

Citrate -> H2O + cis_Aconitate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate
PH: 7.4
Temperature: 36
- [aconitate hydratase(Enzyme) wildtype early aconitase (5h culture age)]

Isocitrate -> cis_Aconitate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate
PH: 7.4
Temperature: 36
- [aconitate hydratase(Enzyme) wildtype late aconitase (12h culture age)]

Isocitrate -> H2O + cis_Aconitate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M phosphate
PH: 7.4
Temperature: 36

Pathways

pathway id name
BioCyc:META_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:META_PWY-6549 L-glutamine biosynthesis III
BioCyc:META_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECO_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:ECOO157_PWY0-42 2-methylcitrate cycle I
BioCyc:MOUSE_TCA-1 TCA cycle
BioCyc:ECOL316407_TCA TCA cycle I (prokaryotic)
BioCyc:META_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
BioCyc:META_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:META_TCA TCA cycle I (prokaryotic)
BioCyc:META_PWY0-42 2-methylcitrate cycle I
BioCyc:ECO_PWY0-42 2-methylcitrate cycle I
BioCyc:ECO_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:YEAST_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:MTBH37RV_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:MTBH37RV_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:HPY_REDCITCYC TCA cycle VIII (helicobacter)
BioCyc:MOUSE_PWY-5690-1 TCA cycle variation III (eukaryotic)
BioCyc:BSUB_FERMENTATION-PWY mixed acid fermentation
BioCyc:BSUB_PWY0-42 2-methylcitrate cycle I
BioCyc:BSUB_ANARESP1-PWY respiration (anaerobic)
BioCyc:BSUB_TCA TCA cycle I (prokaryotic)
BioCyc:ECOL316407_ANARESP1-PWY respiration (anaerobic)
BioCyc:CORYNE_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:HUMAN_PWY66-398 TCA cycle
BioCyc:META_PWY66-398 TCA cycle III (animals)
BioCyc:META_PWY-6969 TCA cycle V (2-oxoglutarate:ferredoxin oxidoreductase)
BioCyc:ECO_TCA TCA cycle I (prokaryotic)
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:MTBH37RV_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_FERMENTATION-PWY mixed acid fermentation
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOL316407_PWY0-42 2-methylcitrate cycle I
BioCyc:CORYNE_TCA TCA cycle I (prokaryotic)
BioCyc:CORYNE_FERMENTATION-PWY mixed acid fermentation
BioCyc:META_PWY-561 superpathway of glyoxylate cycle and fatty acid degradation
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_FERMENTATION-PWY mixed acid fermentation
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:ECO_FERMENTATION-PWY mixed acid fermentation
BioCyc:YEAST_PWY-5690 TCA cycle II (plants and fungi)
BioCyc:MTBH37RV_PWY0-42 2-methylcitrate cycle I
BioCyc:ECOL316407_GLYOXYLATE-BYPASS glyoxylate cycle
BioCyc:ECOL316407_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
BioCyc:CORYNE_TCA-GLYOX-BYPASS superpathway of glyoxylate bypass and TCA
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000802 TCA Cycle
PathBank:SMP0000957 Propanoate Metabolism
PathBank:SMP0000983 Secondary Metabolites: Glyoxylate Cycle
PathBank:SMP0001018 TCA cycle (ubiquinol-2)
PathBank:SMP0001019 TCA cycle (ubiquinol-3)
PathBank:SMP0001020 TCA cycle (ubiquinol-4)
PathBank:SMP0001021 TCA cycle (ubiquinol-5)
PathBank:SMP0001022 TCA cycle (ubiquinol-6)
PathBank:SMP0001023 TCA cycle (ubiquinol-7)
PathBank:SMP0001024 TCA cycle (ubiquinol-8)
PathBank:SMP0001025 TCA cycle (ubiquinol-9)
PathBank:SMP0001026 TCA cycle (ubiquinol-10)
PathBank:SMP0002035 Glycolate and Glyoxylate Degradation II
PathBank:SMP0002037 TCA cycle (ubiquinol-0)
Reactome:R-DDI-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-MMU-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-RNO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-BTA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-CEL-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-HSA-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SCE-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-SPO-9854311 Maturation of TCA enzymes and regulation of TCA cycle
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-BTA-71403 Citric acid cycle (TCA cycle)
Reactome:R-DDI-382551 Transport of small molecules
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-382551 Transport of small molecules
Reactome:R-RNO-917937 Iron uptake and transport
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SPO-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-389542 NADPH regeneration
Reactome:R-CEL-382551 Transport of small molecules
Reactome:R-DDI-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-8978934 Metabolism of cofactors
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-389542 NADPH regeneration
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-389542 NADPH regeneration
Reactome:R-RNO-196854 Metabolism of vitamins and cofactors
Reactome:R-RNO-389542 NADPH regeneration
Reactome:R-SCE-1430728 Metabolism
Reactome:R-CEL-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-CEL-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-8978934 Metabolism of cofactors
Reactome:R-CEL-917937 Iron uptake and transport
Reactome:R-DDI-389542 NADPH regeneration
Reactome:R-DDI-917937 Iron uptake and transport
Reactome:R-HSA-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-HSA-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-8978934 Metabolism of cofactors
Reactome:R-MMU-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-MMU-71403 Citric acid cycle (TCA cycle)
Reactome:R-MMU-8978934 Metabolism of cofactors
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-RNO-71403 Citric acid cycle (TCA cycle)
Reactome:R-RNO-8978934 Metabolism of cofactors
Reactome:R-SCE-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-SCE-71403 Citric acid cycle (TCA cycle)
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-196854 Metabolism of vitamins and cofactors
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-1428517 Aerobic respiration and respiratory electron transport
Reactome:R-DDI-71403 Citric acid cycle (TCA cycle)
Reactome:R-HSA-382551 Transport of small molecules
Reactome:R-HSA-917937 Iron uptake and transport
Reactome:R-HSA-1430728 Metabolism
Reactome:R-MMU-382551 Transport of small molecules
Reactome:R-MMU-917937 Iron uptake and transport
WikiPathways:WP2624 TCA cycle (Krebs cycle)
WikiPathways:WP5355 Metabolic Epileptic Disorders
PathBank:SMP0121182 Citrate Cycle
PathBank:SMP0000548 Mitochondrial Complex II Deficiency
PathBank:SMP0002295 The Oncogenic Action of Fumarate
PathBank:SMP0002358 The Oncogenic Action of L-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0087270 Warburg Effect
PathBank:SMP0108771 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0000547 Fumarase Deficiency
PathBank:SMP0000551 Pyruvate Dehydrogenase Deficiency (E2)
PathBank:SMP0002292 The Oncogenic Action of Succinate
PathBank:SMP0002336 Glyoxylate Cycle
PathBank:SMP0000057 Citric Acid Cycle
PathBank:SMP0000549 2-Ketoglutarate Dehydrogenase Complex Deficiency
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0002359 The Oncogenic Action of D-2-Hydroxyglutarate in Hydroxyglutaric aciduria
PathBank:SMP0002414 TCA Cycle
PathBank:SMP0002426 Glutamine Metabolism
PathBank:SMP0000546 Congenital Lactic Acidosis
PathBank:SMP0000550 Pyruvate Dehydrogenase Deficiency (E3)
PathBank:SMP0002291 The Oncogenic Action of 2-Hydroxyglutarate
PathBank:SMP0002298 Glutaminolysis and Cancer
PathBank:SMP0087363 Warburg Effect
PathBank:SMP0087527 Warburg Effect