EC: 4.2.1.11

phosphopyruvate hydratase (2-phospho-D-glycerate hydro-lyase (phosphoenolpyruvate-forming))

enzyme lambda metabolic reaction experiment
uniprot:P00925 [phosphopyruvate hydratase(Enzyme) wildtype isozyme Eno2p]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (ENO2 * ENOENO2kcat) ) * (( (P2G / ENOENO2Kp2g) ) - (PEP / (ENOENO2Kp2g * KeqENO)))) ) / (( (1 + (P2G / ENOENO2Kp2g)) ) + (PEP / ENOENO2Kpep))) buffer: *
PH: 7
Temperature: 36
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype isozyme Eno1p]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (ENO1 * ENOENO1kcat) ) * (( (P2G / ENOENO1Kp2g) ) - (PEP / (ENOENO1Kp2g * KeqENO)))) ) / (( (1 + (P2G / ENOENO1Kp2g)) ) + (PEP / ENOENO1Kpep))) buffer: *
PH: 7
Temperature: 36
uniprot:Q97QS2 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 10 mM MgCl2, 7.7 mM KCl
PH: 7.5
Temperature: 22
uniprot:Q8DTS9 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-Cl, 2 mM MgSO4, 0.27 mM disodium ethylenediaminetetraacetate
PH: 7.3
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-Cl, 2 mM MgSO4, 0.27 mM disodium ethylenediaminetetraacetate
PH: 7.4
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-Cl, 2 mM MgSO4, 0.27 mM disodium ethylenediaminetetraacetate
PH: 7.4
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-Cl, 2 mM MgSO4, 0.27 mM disodium ethylenediaminetetraacetate
PH: 7.7
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 0.2 M KCl, 1 mM MgSO4, 0.01 mM EDTA, 0.05 M Imidazole-HCl
PH: 7
Temperature: 25
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 0.2 M KCl, 1 mM MgSO4, 0.01 mM EDTA, 0.05 M Tris-HCl
PH: 8
Temperature: 25
uniprot:P0A6P9 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 0.2 M KCl, 1 mM MgSO4, 0.01 mM EDTA, 0.05 M Tris-HCl
PH: 8
Temperature: 25
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 0.2 M KCl, 1 mM MgSO4, 0.01 mM EDTA, 0.05 M Imidazole-HCl
PH: 7
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 6.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 7.1
Temperature: 25
uniprot:P25704 [Li+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 7.1
Temperature: 25
uniprot:P25704 [Li+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 6.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 7.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM Tris, 25 mM MES
PH: 7.1
Temperature: 25
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM HEPES, 10 mM Magnesiumsulfate
PH: 7
Temperature: 37
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 300 mM potassium, 245 mM glutamate, 50 mM phosphate, 20 mM sodium, 2 mM magnesium, 2.5-10 mM sulphate, 0.5 mM calcium
PH: 6.8
Temperature: 30
uniprot:Q9LEE0 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.15 M Tris-HCl
PH: 7.8
Temperature: 22
uniprot:Q9LEE0 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.15 M Tris-HCl
PH: 7.8
Temperature: 22
uniprot:Q27727 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (( (( (Vpf * VPFvENO) ) * p2gPF) ) / (Kp2gPFvENO * Vpf)) ) * (1 - (pepPF / (p2gPF * KeqPFvENO)))) ) / (( (1 + (p2gPF / (Kp2gPFvENO * Vpf))) ) + (pepPF / (KpepPFvENO * Vpf)))) buffer: *
PH: 7
Temperature: 36
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (cytosol * Vmax_10) ) * (( (P2G / Kp2g_10) ) - (PEP / (Kp2g_10 * Keq_10)))) ) / (( (1 + (P2G / Kp2g_10)) ) + (PEP / Kpep_10))) buffer: *
PH: 7
Temperature: 36
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype isozyme Eno1p, C-terminal GST-tag]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MES, 100 mM KCl, 0.15 mM NADH, 10 mM ADP, 14 U/ml pyruvate kinase, 20 U/ml lactate dehydrogenase, 5 mM MgCl2
PH: 6.5
Temperature: 30
uniprot:P00925 [phosphopyruvate hydratase(Enzyme) wildtype isozyme Eno2p, N-terminal GST-tag]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MES, 100 mM KCl, 0.15 mM NADH, 10 mM ADP, 14 U/ml pyruvate kinase, 20 U/ml lactate dehydrogenase, 5 mM MgCl2
PH: 6.5
Temperature: 30
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype isozyme Eno1p, C-terminal GST-tag]

3_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 100 mM MES, 100 mM KCl, 0.15 mM NADH, 10 mM ADP, 14 U/ml pyruvate kinase, 20 U/ml lactate dehydrogenase, 5 mM MgCl2
PH: 6.5
Temperature: 30
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (cytosol * VmENO) ) / KmENOP2G) ) * (P2G - (PEP / KeqENO))) ) / (( (1 + (P2G / KmENOP2G)) ) + (PEP / KmENOPEP))) buffer: *
PH: 7
Temperature: 36
uniprot:P0CX11 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Tris, 25 mM 2-(N-morpholino)ethanesulphonic acid (MES)
PH: 7.1
Temperature: 36
uniprot:P42222 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Tris, 25 mM 2-(N-morpholino)ethanesulphonic acid (MES)
PH: 7.1
Temperature: 36
uniprot:P00924 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 25 mM Tris, 25 mM 2-(N-morpholino)ethanesulphonic acid (MES)
PH: 7.1
Temperature: 36
- [Ca2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * M) ) / (( (Km_M * (1 + (I / Ki))) ) + M)) buffer: 0.05 M Imidazole, 0.4 M KCl
PH: 7
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Imidazole, 0.4 M KCl
PH: 7
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 0.05 M Imidazole, 0.4 M KCl
PH: 7
Temperature: 36
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 0.05 M Imidazole, 0.4 M KCl
PH: 7
Temperature: 36
uniprot:P0A6P9 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.05 M Tris-HCl, 0.1 M KCl, 0.01 mM EDTA
PH: 8.1
Temperature: 30
uniprot:P00924 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * M) ) / (Km + (( (M * (1 + (M / Kin))) ) / (1 + (M / Kid))))) buffer: 0.05 M Hepes
PH: 7.5
Temperature: 36
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype ENO1]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * M) ) / (Km + (( (M * (1 + (M / Kin))) ) / (1 + (M / Kid))))) buffer: 0.05 M Hepes
PH: 7.5
Temperature: 36
uniprot:P00924 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * M) ) / (Km + (( (M * (1 + (M / Kin))) ) / (1 + (M / Kid))))) buffer: 0.05 M Hepes
PH: 7.5
Temperature: 36
uniprot:P00924 [Co2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * M) ) / (Km + (( (M * (1 + (M / Kin))) ) / (1 + (M / Kid))))) buffer: 0.05 M Hepes
PH: 7.5
Temperature: 36
uniprot:P00924 [Mg2+]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (( (E * kcat) ) * M) ) / (Km + (( (M * (1 + (M / Kin))) ) / (1 + (M / Kid))))) buffer: 0.05 M Hepes
PH: 7.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) * (1 + (Ka / M)))) buffer: 0.5 M Potassium chloride, 0.05 M Tris-Cl
PH: 7.5
Temperature: 30
- [Mn2+]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (Vmax / (( (1 + (Km / S)) ) * (1 + (Ka / M)))) buffer: 0.5 M Potassium chloride, 0.05 M Tris-Cl
PH: 7.5
Temperature: 30
- [Mg2+]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (Vmax / (( (1 + (Km / S)) ) * (1 + (Ka / M)))) buffer: 0.5 M Potassium chloride, 0.05 M Tris-Cl
PH: 7.5
Temperature: 30
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) * (1 + (Ka / M)))) buffer: 0.5 M Potassium chloride, 0.05 M Tris-Cl
PH: 7.5
Temperature: 30
uniprot:Q9UAE6 [phosphopyruvate hydratase(Enzyme) wildtype ENO1]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole, 1.1 mM MgCl2, 0.1 mM EDTA, 250 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q9UAE6 [phosphopyruvate hydratase(Enzyme) mutant ENO1deltaEK]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole, 1.1 mM MgCl2, 0.1 mM EDTA, 250 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q9BPL7 [phosphopyruvate hydratase(Enzyme) wildtype ENO2]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole, 1.1 mM MgCl2, 0.1 mM EDTA, 250 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q9UAE6 [phosphopyruvate hydratase(Enzyme) mutant ENO1deltaEWGYS]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole, 1.1 mM MgCl2, 0.1 mM EDTA, 250 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q9UAE6 [phosphopyruvate hydratase(Enzyme) mutant ENO1deltaEKdeltaEWGYS]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole, 1.1 mM MgCl2, 0.1 mM EDTA, 250 mM KCl
PH: 7.2
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 6.4
Temperature: 37
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 7.5
Temperature: 37
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 6.9
Temperature: 37
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 6.9
Temperature: 37
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 6.4
Temperature: 37
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (V1 * A) ) / Ka) ) - (( (V2 * P) ) / Kp)) ) / (( (1 + (A / Ka)) ) + (P / Kp))) buffer: 0.1 M Imidazole-HCl, 8 mM MgSO4, 0.2 M KCl
PH: 7.5
Temperature: 37
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl
PH: 7.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Bis-Tris, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM PIPES, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl
PH: 6.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM DMG, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM DMG, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM HEPES, 100 mM KCl
PH: 7
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Cacodylate, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM DMG, 100 mM KCl
PH: 5.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM DMG, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM PIPES, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM MES, 100 mM KCl
PH: 5.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM MES, 100 mM KCl
PH: 5.15
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM PIPES, 100 mM KCl
PH: 5.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (Ka + B)) buffer: 50 mM Cacodylate, 100 mM KCl
PH: 5.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM DMG, 100 mM KCl
PH: 5.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM MES, 100 mM KCl
PH: 5.15
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM HEPES, 100 mM KCl
PH: 7.5
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM MES, 100 mM KCl
PH: 6.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM DMG, 100 mM KCl
PH: 6.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM PIPES, 100 mM KCl
PH: 6.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM HEPES, 100 mM KCl
PH: 7
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM PIPES, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM HEPES, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM PIPES, 100 mM KCl
PH: 7
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM HEPES, 100 mM KCl
PH: 6.6
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Cacodylate, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Bis-Tris, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM MES, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Bis-Tris, 100 mM KCl
PH: 5.8
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM DMG, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * B) ) / (( (Ka + B) ) + (( (B ^ 2) ) / Ki))) buffer: 50 mM Cacodylate, 100 mM KCl
PH: 6.2
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 5.43
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 5.97
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 5.75
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.52
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.26
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.37
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.78
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.74
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 6.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM TAPS, 50 mM KCl
PH: 7.81
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM MES, 50 mM KCl
PH: 6.73
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9.25
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.29
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.75
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.02
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (( (1 + (Km / S)) ) + (S / Ki))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.51
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.75
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.43
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.73
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.52
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.97
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.37
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.26
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.74
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.75
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.78
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.29
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.51
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.02
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 7.81
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9.25
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.97
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.52
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.24
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.75
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.73
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 6.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM MES, 50 mM KCl
PH: 5.43
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.78
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.51
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.29
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.26
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 7.37
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 8.02
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM HEPES, 50 mM KCl
PH: 6.74
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 7.81
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9.25
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 9
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.54
Temperature: 36
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (Vmax / (1 + (Km / S))) buffer: 50 mM TAPS, 50 mM KCl
PH: 8.75
Temperature: 36
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (Vmax * S) ) + (( (( (V2max * S) ) * S) ) / Ki)) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM MES, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (Vmax * S) ) + (( (( (V2max * S) ) * S) ) / Ki)) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM MES, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (Vmax * S) ) + (( (( (V2max * S) ) * S) ) / Ki)) ) / (( (Km + S) ) + (( (S * S) ) / Ki))) buffer: 25 mM MES, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM MES, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 2 mM MgSO4, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1 mM ATP, 0.42 mM NADH, 0.1 mM 2,3-bisphosphoglycerate, 2 mM MgSO4, 1 mM dithiothreitol, 2 U/ml Phosphoglycerate mutase from rabbit muscle, 0.025 mg/ml Phosphoglycerate kinase from yeast, 8 U/ml Glyceraldehyde 3-phosphate dehydrogenase from rabbit muscle
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 2 mM MgSO4, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 2 mM MgSO4, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [phosphopyruvate hydratase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1 mM ATP, 0.42 mM NADH, 0.1 mM 2,3-bisphosphoglycerate, 2 mM MgSO4, 1 mM dithiothreitol, 2 U/ml Phosphoglycerate mutase from rabbit muscle, 0.025 mg/ml Phosphoglycerate kinase from yeast, 8 U/ml Glyceraldehyde 3-phosphate dehydrogenase from rabbit muscle
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [phosphopyruvate hydratase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1 mM ATP, 0.42 mM NADH, 0.1 mM 2,3-bisphosphoglycerate, 2 mM MgSO4, 1 mM dithiothreitol, 2 U/ml Phosphoglycerate mutase from rabbit muscle, 0.025 mg/ml Phosphoglycerate kinase from yeast, 8 U/ml Glyceraldehyde 3-phosphate dehydrogenase from rabbit muscle
PH: 7.6
Temperature: 25
uniprot:P00924 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * M) ) / (( (Ka + M) ) + (( (M ^ 2) ) / Ki))) buffer: 0.1 M triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * M) ) / (( (Ka + M) ) + (( (M ^ 2) ) / Ki))) buffer: 0.1 M triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:Q38BV6 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * M) ) / (( (Ka + M) ) + (( (M ^ 2) ) / Ki))) buffer: 0.1 M triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1.1 mM ADP, 0.42 mM NADH, 2 mM MgSO4, 17 mM KCl, 2 U/ml Pyruvate kinase from rabbit muscle, 1.2 U/ml Lactate dehydrogenase from beef heart
PH: 7.6
Temperature: 25
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Triethanolamine/HCl, 1 mM ATP, 0.42 mM NADH, 0.1 mM 2,3-bisphosphoglycerate, 2 mM MgSO4, 1 mM dithiothreitol, 2 U/ml Phosphoglycerate mutase from rabbit muscle, 0.025 mg/ml Phosphoglycerate kinase from yeast, 8 U/ml Glyceraldehyde 3-phosphate dehydrogenase from rabbit muscle
PH: 7.6
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole
PH: 7.1
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole
PH: 7.1
Temperature: 25
- [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole
PH: 7.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype enolase]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) mutant Enolase G37A]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Co2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Co2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) mutant Enolase G41A]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Co2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * A) ) / (( (Km + A) ) + (( (A ^ 2) ) / Ki))) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM Mes, 25 mM Tris
PH: 7.1
Temperature: 25
uniprot:P04764 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (V * S) ) / (Km + S)) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38
uniprot:P04764 [phosphopyruvate hydratase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (V * S) ) / (Km + S)) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38
uniprot:P04764 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (( (Vf * S) ) / Kms) ) - (( (Vr * P) ) / Kmp)) ) / (( (1 + (S / Kms)) ) + (P / Kmp))) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38
uniprot:Q1KYT0 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole, 400 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q1KYT0 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole, 400 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q1KYT0 [Mg2+]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Imidazole, 400 mM KCl
PH: 7.2
Temperature: 25
uniprot:Q8IJN7 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 1.5 mM MgCl2
PH: 7.4
Temperature: 20
uniprot:Q8IJN7 [phosphopyruvate hydratase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris/HCl, 1.5 mM MgCl2
PH: 7.4
Temperature: 20
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris Cl, 10E-3 M MgCl2
PH: 7.81
Temperature: 30
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.05 M Tris Cl, 10E-3 M MgCl2
PH: 7.81
Temperature: 30
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
- [phosphopyruvate hydratase(Enzyme) wildtype]

3_Chloro_2_phospholactate -> HCl + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
- [phosphopyruvate hydratase(Enzyme) wildtype]

3_Chloro_2_phospholactate -> Phosphoenolpyruvate + HCl		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

3_Chloro_2_phospholactate -> HCl + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

3_Chloro_2_phospholactate -> HCl + Phosphoenolpyruvate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
- [phosphopyruvate hydratase(Enzyme) wildtype]

3_Fluoro_2_phospholactate -> Phosphoenolpyruvate + HF		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

3_Fluoro_2_phospholactate -> Phosphoenolpyruvate + HF		 (( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Tris, 5 mM MgSO4, 0.4 M KCl, 0.01 mM EDTA
PH: 7.8
Temperature: 25
uniprot:P00924 [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Co2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Mn2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (M * kcat) ) * E) ) / (( (kcat / kcat_Km) ) + M)) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (M * kcat) ) * E) ) / (( (kcat / kcat_Km) ) + M)) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) mutant S39A]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Co2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P00924 [Ni2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (M * kcat) ) * E) ) / (( (( (kcat / kcat_Km) ) + M) ) + (( (M * M) ) / Ki))) buffer: 0.05 M Hepes/KOH
PH: 7.5
Temperature: 36
uniprot:P25704 [phosphopyruvate hydratase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 37
- [phosphopyruvate hydratase(Enzyme) wildtype]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
- [phosphopyruvate hydratase(Enzyme) wildtype]

Phosphoenolpyruvate + H2O -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: -
PH: 7.6
Temperature: 30
uniprot:Q1ET73 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM imidazole-HCl, 400 mM KCl
PH: 6.8
Temperature: 30
uniprot:Q1ET73 [Mn2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole-HCl, 400 mM KCl
PH: 6.8
Temperature: 30
uniprot:Q1ET73 [phosphopyruvate hydratase(Enzyme) wildtype His-tagged]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole-HCl, 400 mM KCl
PH: 6.8
Temperature: 30
uniprot:Q1ET73 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * M) ) / (Km + M)) buffer: 50 mM imidazole-HCl, 400 mM KCl
PH: 6.8
Temperature: 30
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype Enolase 1]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (kcat * Et) ) * S) ) / (Ks + S)) buffer: 100.0 mM 2-(N-morpholino)ethanesulfonic acid, 5.0 mM magnesium dichloride, NADH, 100.0 mM potassium chloride, 10.0 mM ADP
PH: 6.5
Temperature: 30
uniprot:P00924 [phosphopyruvate hydratase(Enzyme) wildtype Enolase 1]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (( (kcat * Et) ) * S) ) / (Ks + S)) buffer: 100.0 mM 2-(N-morpholino)ethanesulfonic acid, 5.0 mM magnesium dichloride, NADH, 100.0 mM potassium chloride, 10.0 mM ADP
PH: 6.5
Temperature: 30
uniprot:P51555 [phosphopyruvate hydratase(Enzyme) wildtype His-tagged]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 0.15 mM NADH, 0.45 U pyruvate kinase-lactate dehydrogenase, 0.9 mM ADP, 1 mM 2-phosphoglycerate
PH: 7
Temperature: 37
uniprot:P51555 [phosphopyruvate hydratase(Enzyme) wildtype His-tagged]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 0.15 mM NADH, 0.45 U pyruvate kinase-lactate dehydrogenase, 0.9 mM ADP, 1 mM 2-phosphoglycerate
PH: 6
Temperature: 37
uniprot:P51555 [phosphopyruvate hydratase(Enzyme) wildtype His-tagged]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 1 mM EDTA, 2 mM dithiothreitol, 0.15 mM NADH, 1 mM GTP, 0.2 U phosphoglycerate mutase, 1.2 U phosphoglyerate kinase, 1.6 U glyceraldehyde-3-phosphate dehydrogenase
PH: 7
Temperature: 37
uniprot:P51555 [phosphopyruvate hydratase(Enzyme) wildtype His-tagged]

H2O + Phosphoenolpyruvate -> 2_Phospho_D_glycerate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM imidazole, 10 mM Acetic acid/Mes/Tris, 5 mM MgCl2, 1 mM EDTA, 2 mM dithiothreitol, 0.15 mM NADH, 1 mM GTP, 0.2 U phosphoglycerate mutase, 1.2 U phosphoglyerate kinase, 1.6 U glyceraldehyde-3-phosphate dehydrogenase
PH: 6
Temperature: 37
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-chloride, 27 mM Glycine
PH: 7.4
Temperature: 20
uniprot:P25704 [Mg2+]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-chloride, 27 mM Glycine
PH: 7.4
Temperature: 20
uniprot:P00924 [Potassium bromide]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: Tris-Acetate, 1.0 mM Magnesium acetate, 1E-05 M EDTA
PH: 7.4
Temperature: 30
uniprot:Q5XD01 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM HEPES, 3.3 mM MgSO4, 7.7 mM KCl
PH: 7
Temperature: 37
- [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * A) ) / (Km + A)) buffer: 30 mM triethanolamine, 30 mM KCl, 3 mM MgCl2, 0.5 mM ADP, 10 units/ml pyruvate kinase, 10 units/ml lactate dehydrogenase
PH: 7.5
Temperature: 25
uniprot:Q97QS2 [phosphopyruvate hydratase(Enzyme) wildtype]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM HEPES, 10 mM MgCl2, 7.7 mM KCl
PH: 7
Temperature: 37
uniprot:Q3ZC09 [Mg2+]

2_Phospho_D_glycerate -> H2O + Phosphoenolpyruvate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 7.4
Temperature: 22
uniprot:P37869 [phosphopyruvate hydratase(Enzyme) wildtype His-tag]

2_Phospho_D_glycerate -> Phosphoenolpyruvate + H2O		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 20 mM Tris-HCl, 2 mM MgCl2, 400 mM KCl
PH: 8
Temperature: 22

Pathways

pathway id name
BioCyc:HUMAN_PWY66-400 glycolysis
BioCyc:META_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:META_PWY-6886 1-butanol autotrophic biosynthesis (engineered)
BioCyc:META_P341-PWY glycolysis V (Pyrococcus)
BioCyc:META_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:ECO_GLUCONEO-PWY gluconeogenesis I
BioCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:VCHO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:MTBCDC1551_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:MTBCDC1551_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:HPY_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOO157_PWY-7003 glycerol degradation to butanol
BioCyc:ECOO157_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOO157_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:ECOL199310_ANARESP1-PWY respiration (anaerobic)
BioCyc:CAULO_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:CAULO_GLUCONEO-PWY gluconeogenesis I
BioCyc:CAULO_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:SYNEL_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:SYNEL_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ECOL316407_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOL316407_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:CAULONA1000_ANAGLYCOLYSIS-PWY glycolysis III
BioCyc:CAULONA1000_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:BTHE_PWY-6901 superpathway of glucose and xylose degradation
BioCyc:BTHE_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:BTHE_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:BTHE_PWY66-400 glycolysis VI (metazoan)
BioCyc:GCF_000013425_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:GCF_000013425_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:GCF_000013425_GLUCONEO-PWY gluconeogenesis I
BioCyc:HUMAN_PWY66-399 gluconeogenesis
BioCyc:META_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
BioCyc:META_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:META_PWY-6142 gluconeogenesis II (Methanobacterium thermoautotrophicum)
BioCyc:META_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:META_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:META_PWY-8004 Entner-Doudoroff pathway I
BioCyc:META_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:META_PWY-7345 superpathway of anaerobic sucrose degradation
BioCyc:ECO_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:YEAST_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:ARA_PWY-1042 glycolysis IV (plant cytosol)
BioCyc:ARA_GLUCONEO-PWY gluconeogenesis I
BioCyc:VCHO_FAO-PWY fatty acid β-oxidation I
BioCyc:MTBH37RV_GLUCONEO-PWY gluconeogenesis I
BioCyc:MTBCDC1551_GLUCONEO-PWY gluconeogenesis I
BioCyc:MTBCDC1551_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:ECOO157_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:ECOO157_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:ECOL199310_PWY-6901 glucose and xylose degradation
BioCyc:ECOL199310_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:ECOL199310_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:CAULO_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ANTHRA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ANTHRA_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:ANTHRA_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:BSUB_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:BSUB_ANARESP1-PWY respiration (anaerobic)
BioCyc:SYNEL_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL316407_ANARESP1-PWY respiration (anaerobic)
BioCyc:ECOL316407_GLUCONEO-PWY gluconeogenesis I
BioCyc:CAULONA1000_GLUCONEO-PWY gluconeogenesis I
BioCyc:PDIF272563_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:CORYNE_GLUCONEO-PWY gluconeogenesis I
BioCyc:GCF_000013425_P122-PWY heterolactic fermentation
BioCyc:HUMAN_PWY66-407 superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_PWY66-399 gluconeogenesis III
BioCyc:META_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_PWY-6146 Methanobacterium thermoautotrophicum biosynthetic metabolism
BioCyc:ECO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:YEAST_GLYCOLYSIS-YEAST-PWY glycolysis
BioCyc:YEAST_GLUCONEO-YEAST-PWY gluconeogenesis
BioCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_PWY66-399 gluconeogenesis III
BioCyc:VCHO_P441-PWY superpathway of N-acetylneuraminate degradation
BioCyc:VCHO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:MTBH37RV_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:MTBCDC1551_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:HPY_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECOO157_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:ECOO157_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:ECOO157_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOO157_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:ECOL199310_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL199310_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL199310_HEXITOLDEGSUPER-PWY superpathway of hexitol degradation (bacteria)
BioCyc:CAULO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:CAULO_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ANTHRA_PWY-1622 formaldehyde assimilation I (serine pathway)
BioCyc:ANTHRA_GLUCONEO-PWY gluconeogenesis I
BioCyc:BSUB_GLUCONEO-PWY-1 gluconeogenesis I
BioCyc:BSUB_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECOL316407_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:CAULONA1000_PWY-1622 formaldehyde assimilation I (serine pathway)
BioCyc:CAULONA1000_GLYCOLYSIS glycolysis I
BioCyc:PDIF272563_GLUCONEO-PWY gluconeogenesis I
BioCyc:META_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:META_PWY-5723 Rubisco shunt
BioCyc:META_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:META_P461-PWY hexitol fermentation to lactate, formate, ethanol and acetate
BioCyc:META_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:META_PWY-7124 ethylene biosynthesis V (engineered)
BioCyc:ECO_GLYCOLYSIS-E-D superpathway of glycolysis and the Entner-Doudoroff pathway
BioCyc:ECO_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:ARA_PWY-5723 Rubisco shunt
BioCyc:VCHO_GLUCONEO-PWY gluconeogenesis I
BioCyc:VCHO_GLYCOLYSIS-TCA-GLYOX-BYPASS superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass
BioCyc:VCHO_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:MTBCDC1551_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:HPY_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOO157_PWY-6901 glucose and xylose degradation
BioCyc:ECOO157_GLYCOLYSIS glycolysis I (from glucose-6P)
BioCyc:ECOO157_ANARESP1-PWY respiration (anaerobic)
BioCyc:ECOO157_GLYCOLYSIS-E-D superpathway of glycolysis and Entner-Doudoroff
BioCyc:ECOL199310_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:CAULO_ANARESP1-PWY respiration (anaerobic)
BioCyc:CAULO_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:ANTHRA_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:BSUB_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:SYNEL_PWY-5723 Rubisco shunt
BioCyc:SYNEL_GLUCONEO-PWY gluconeogenesis I
BioCyc:ECOL316407_PWY-5484 glycolysis II (from fructose-6P)
BioCyc:CAULONA1000_ANARESP1-PWY respiration (anaerobic)
BioCyc:BTHE_GLUCONEO-PWY gluconeogenesis I
BioCyc:CORYNE_ANAEROFRUCAT-PWY homolactic fermentation
BioCyc:CORYNE_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
BioCyc:CORYNE_PWY-5484 glycolysis II (from fructose 6-phosphate)
BioCyc:CORYNE_ANAGLYCOLYSIS-PWY glycolysis III (from glucose)
BioCyc:GCF_000013425_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
WikiPathways:WP2472 Escherichia coli K-12 peripherome
PathBank:SMP0000807 Glycolysis and Pyruvate Dehydrogenase
PathBank:SMP0000816 D-Glucarate and D-Galactarate Degradation
PathBank:SMP0000839 Gluconeogenesis from L-Malic Acid
PathBank:SMP0000930 Fructose Metabolism
PathBank:SMP0000931 Glycerol Metabolism
PathBank:SMP0000932 Glycerol Metabolism II
PathBank:SMP0000933 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000934 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000935 Glycerol Metabolism V (Glycerophosphoserine)
PlantCyc:ARA_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
PlantCyc:RUBBERTREE_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:RUBBERTREE_GLUCONEO-PWY gluconeogenesis I
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-1042 glycolysis IV (plant cytosol)
PlantCyc:PLANT_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:ARA_PWY66-399 gluconeogenesis III
PlantCyc:ARA_PWY-5723 Rubisco shunt
PlantCyc:ARA_GLYCOLYSIS glycolysis I (from glucose 6-phosphate)
PlantCyc:ARA_GLUCONEO-PWY gluconeogenesis I
PlantCyc:PLANT_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:PLANT_PWY-5723 Rubisco shunt
PlantCyc:PLANT_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:ARA_PWY-5484 glycolysis II (from fructose 6-phosphate)
PlantCyc:ARA_PWY-1042 glycolysis IV (plant cytosol)
PlantCyc:RUBBERTREE_PWY-5723 Rubisco shunt
PlantCyc:RUBBERTREE_PWY-1042 glycolysis IV
PlantCyc:PLANT_PWY-5464 superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle
Reactome:R-HSA-9635486 Infection with Mycobacterium tuberculosis
Reactome:R-HSA-9636667 Manipulation of host energy metabolism
Reactome:R-HSA-9637690 Response of Mtb to phagocytosis
Reactome:R-HSA-9824439 Bacterial Infection Pathways
Reactome:R-BTA-1430728 Metabolism
Reactome:R-CEL-70263 Gluconeogenesis
Reactome:R-DRE-70171 Glycolysis
Reactome:R-DME-71387 Carbohydrate metabolism
Reactome:R-DME-70326 Glucose metabolism
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-352875 Gluconeogenesis
Reactome:R-HSA-70263 Gluconeogenesis
Reactome:R-HSA-1643685 Disease
Reactome:R-MMU-1430728 Metabolism
Reactome:R-PFA-70171 Glycolysis
Reactome:R-RNO-70171 Glycolysis
Reactome:R-HSA-70171 Glycolysis
Reactome:R-BTA-70171 Glycolysis
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-CEL-70326 Glucose metabolism
Reactome:R-DRE-70263 Gluconeogenesis
Reactome:R-DME-1430728 Metabolism
Reactome:R-GGA-352882 Glycolysis
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-70326 Glucose metabolism
Reactome:R-MMU-70171 Glycolysis
Reactome:R-PFA-70263 Gluconeogenesis
Reactome:R-RNO-70263 Gluconeogenesis
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-BTA-70326 Glucose metabolism
Reactome:R-CEL-70171 Glycolysis
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DME-70263 Gluconeogenesis
Reactome:R-GGA-353098 Carbohydrate metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-70326 Glucose metabolism
Reactome:R-PFA-1430728 Metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-BTA-70263 Gluconeogenesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-DRE-71387 Carbohydrate metabolism
Reactome:R-DRE-70326 Glucose metabolism
Reactome:R-DME-70171 Glycolysis
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-5663205 Infectious disease
Reactome:R-MMU-70263 Gluconeogenesis
Reactome:R-PFA-71387 Carbohydrate metabolism
Reactome:R-PFA-70326 Glucose metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
Reactome:R-RNO-70326 Glucose metabolism
WikiPathways:WP2621 Glycolysis
PathBank:SMP0120488 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0120637 Glycogenosis, Type IB
PathBank:SMP0120645 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0121308 Fructose Metabolism
PathBank:SMP0121312 Glycerol Metabolism IV (Glycerophosphoglycerol)
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0000572 Fanconi-Bickel Syndrome
PathBank:SMP0120596 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0120624 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0120636 Fanconi-Bickel Syndrome
PathBank:SMP0121311 Glycerol Metabolism III (sn-Glycero-3-Phosphoethanolamine)
PathBank:SMP0000040 Glycolysis
PathBank:SMP0000531 Glycogenosis, Type VII. Tarui Disease
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0063617 Gluconeogenesis
PathBank:SMP0120626 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0120638 Glycogenosis, Type IC
PathBank:SMP0121277 D-Glucarate and D-Galactarate Degradation
PathBank:SMP0121309 Glycerol Metabolism
PathBank:SMP0121313 Glycerol Metabolism V (Glycerophosphoserine)
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0063478 Glycolysis
PathBank:SMP0120627 Triosephosphate Isomerase Deficiency
PathBank:SMP0121310 Glycerol Metabolism II
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC
PathBank:SMP0086930 Warburg Effect