EC: 2.7.1.33

pantothenate kinase (ATP:(R)-pantothenate 4'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q923S8 [pantothenate kinase(Enzyme) wildtype]

(R)_Pantothenate + ATP -> ADP + D_4'_Phosphopantothenate		 (( (V * A) ) / (Km + A)) buffer: 40 mM Tris/maleate, 10 mM MgCl2, 2 mM dithiothreitol, 10 mM sodium/potassium tartrate
PH: 6.1
Temperature: 37
uniprot:P37564 [pantothenate kinase(Enzyme) wildtype PanK type III]

(R)_Pantothenate + ATP -> ADP + D_4'_Phosphopantothenate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM HEPES, 20 mM KCl, 10 mM MgCl2, 2 mM Phosphoenolpyruvate, 0.3 mM NADH, 16.7 U/ml Lactate dehydrogenase, 8.3 U/ml Pyruvate kinase
PH: 7.6
Temperature: 25
uniprot:O25533 [pantothenate kinase(Enzyme) wildtype PanK type III]

ATP + (R)_Pantothenate -> ADP + D_4'_Phosphopantothenate		 (( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KiA * KmB) ) + (KmA * B)) ) + (KmB * A)) ) + (A * B))) buffer: 100 mM HEPES, 20 mM KCl, 10 mM MgCl2, 2 mM Phosphoenolpyruvate, 0.3 mM NADH, 16.7 U/ml Lactate dehydrogenase, 8.3 U/ml Pyruvate kinase
PH: 7.6
Temperature: 25
- [pantothenate kinase(Enzyme) wildtype CoaA]

(R)_Pantothenate + ATP -> D_4'_Phosphopantothenate + ADP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.3 mM NADH, 0.5 mM Phosphoenolpyruvate, 10 mM MgCl2, 20 mM KCl, 0.01 units/µl Pyruvate kinase, 0.01 units/µl Lactic dehydrogenase
PH: 7.6
Temperature: 25
- [pantothenate kinase(Enzyme) wildtype CoaA]

N_Pentylpantothenamide + ATP -> ADP + N_Pentylpantothenamide_4_phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.3 mM NADH, 0.5 mM Phosphoenolpyruvate, 10 mM MgCl2, 20 mM KCl, 0.01 units/µl Pyruvate kinase, 0.01 units/µl Lactic dehydrogenase
PH: 7.6
Temperature: 25
uniprot:Q923S8 [Coenzyme A]

ATP + (R)_Pantothenate -> D_4'_Phosphopantothenate + ADP		 (( (Vmax * A) ) / (Km + (1 + (( (I / (Ki * (1 + (M / Ka)))) ) * A)))) buffer: 50 mM Triethanolamine, 10 mM Dithiothreitol, 6 mM MgCl2
PH: 7
Temperature: 37
uniprot:Q923S8 [pantothenate kinase(Enzyme) wildtype]

ATP + (R)_Pantothenate -> ADP + D_4'_Phosphopantothenate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 5 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q923S8 [Coenzyme A]

ATP + (R)_Pantothenate -> ADP + D_4'_Phosphopantothenate		 (( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 5 mM MgCl2
PH: 7.4
Temperature: 37
uniprot:Q5JHF1 [pantothenate kinase(Enzyme) wildtype N-terminal His-tag]

(R)_Pantothenate + ATP -> ADP + D_4'_Phosphopantothenate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.2 mM NADH, 5 mM phosphoenolpyruvate, 14.8 U/ml PK, 18.6 U/ml LDH, 10 mM MgCl2
PH: 8
Temperature: 42

Pathways

pathway id name
BioCyc:SYNEL_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECOL316407_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:GCF_000013425_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:META_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
BioCyc:ECO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:CAULO_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:HUMAN_COA-PWY-1 coenzyme A biosynthesis
BioCyc:META_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:META_COA-PWY-1 superpathway of coenzyme A biosynthesis III (mammals)
BioCyc:YEAST_PANTO-PWY phosphopantothenate biosynthesis
BioCyc:MTBH37RV_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:MTBCDC1551_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:MTBCDC1551_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:HPY_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:AGRO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:BSUB_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:PDIF272563_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:BTHE_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:META_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:ECO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ARA_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
BioCyc:ARA_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:CAULO_PWY-4221 pantothenate and coenzyme A biosynthesis II
BioCyc:CAULO_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:AGRO_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:BSUB_PANTOSYN-PWY superpathway of coenzyme A biosynthesis I (bacteria)
BioCyc:ECOL316407_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:CORYNE_PANTOSYN-PWY pantothenate and coenzyme A biosynthesis I
BioCyc:CORYNE_PANTO-PWY phosphopantothenate biosynthesis I
BioCyc:GCF_000013425_PANTO-PWY phosphopantothenate biosynthesis I
PathBank:SMP0000848 Pantothenate and CoA Biosynthesis
PlantCyc:ARA_PANTO-PWY phosphopantothenate biosynthesis I
PlantCyc:PLANT_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
PlantCyc:ARA_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
PlantCyc:ARA_PWY-4221 superpathway of coenzyme A biosynthesis II (plants)
PlantCyc:PLANT_PANTO-PWY phosphopantothenate biosynthesis I
PlantCyc:PLANT_PWY-4221ARA superpathway of pantothenate and coenzymeA biosynthesis
Reactome:R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-HSA-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-MMU-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-HSA-196854 Metabolism of vitamins and cofactors
Reactome:R-MMU-196854 Metabolism of vitamins and cofactors
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SCE-199220 Vitamin B5 (pantothenate) metabolism
Reactome:R-HSA-196783 Coenzyme A biosynthesis
Reactome:R-MMU-196783 Coenzyme A biosynthesis
Reactome:R-SPO-196854 Metabolism of vitamins and cofactors
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-196854 Metabolism of vitamins and cofactors
Reactome:R-SPO-196849 Metabolism of water-soluble vitamins and cofactors
Reactome:R-SPO-199220 Vitamin B5 (pantothenate) metabolism
PathBank:SMP0000374 Glycogen Storage Disease Type 1A (GSD1A) or Von Gierke Disease
PathBank:SMP0000560 Phosphoenolpyruvate Carboxykinase Deficiency 1 (PEPCK1)
PathBank:SMP0000563 Triosephosphate Isomerase Deficiency
PathBank:SMP0002307 beta-Alanine Metabolism
PathBank:SMP0000027 Pantothenate and CoA Biosynthesis
PathBank:SMP0000128 Gluconeogenesis
PathBank:SMP0000573 Glycogenosis, Type IB
PathBank:SMP0000581 Glycogenosis, Type IA. Von Gierke Disease
PathBank:SMP0087207 Pantothenate and CoA Biosynthesis
PathBank:SMP0087223 Gluconeogenesis
PathBank:SMP0000562 Fructose-1,6-diphosphatase Deficiency
PathBank:SMP0000574 Glycogenosis, Type IC
PathBank:SMP0002364 Pantothenate and CoA Biosynthesis