EC: 2.7.1.25

adenylyl-sulfate kinase (ATP:adenylyl-sulfate 3'-phosphotransferase)

enzyme lambda metabolic reaction experiment
uniprot:Q43295 [adenylyl-sulfate kinase(Enzyme) wildtype APK1 His-tagged]

Adenylylsulfate + ATP -> 3'_Phosphoadenylyl_sulfate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM MgCl2, 1 mM KCl, 0.3 mM NADH, 0.4 mM PEP, 35 U/ml pyruvate kinase, 50 U/ml lactic dehydrogenase, 70-150 mM ammonium sulfate, 25 U/ml nuclease P1
PH: 8
Temperature: 30
uniprot:Q9SRW7 [adenylyl-sulfate kinase(Enzyme) wildtype APK3 His-tagged]

ATP + Adenylylsulfate -> ADP + 3'_Phosphoadenylyl_sulfate		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM MgCl2, 1 mM KCl, 0.3 mM NADH, 0.4 mM PEP, 35 U/ml pyruvate kinase, 50 U/ml lactic dehydrogenase, 70-150 mM ammonium sulfate, 25 U/ml nuclease P1
PH: 8
Temperature: 30
uniprot:Q84JF0 [adenylyl-sulfate kinase(Enzyme) wildtype APK4 His-tagged]

ATP + Adenylylsulfate -> 3'_Phosphoadenylyl_sulfate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM MgCl2, 1 mM KCl, 0.3 mM NADH, 0.4 mM PEP, 35 U/ml pyruvate kinase, 50 U/ml lactic dehydrogenase, 70-150 mM ammonium sulfate, 25 U/ml nuclease P1
PH: 8
Temperature: 30
uniprot:O49196 [adenylyl-sulfate kinase(Enzyme) wildtype APK2 His-tagged]

Adenylylsulfate + ATP -> 3'_Phosphoadenylyl_sulfate + ADP		 (( (Vmax * S) ) / (Km + S)) buffer: 0.1 M Tris-HCl, 5 mM MgCl2, 1 mM KCl, 0.3 mM NADH, 0.4 mM PEP, 35 U/ml pyruvate kinase, 50 U/ml lactic dehydrogenase, 70-150 mM ammonium sulfate, 25 U/ml nuclease P1
PH: 8
Temperature: 30
uniprot:O43252 [adenylyl-sulfate kinase(Enzyme) wildtype PAPS synthetase isoform 1]

ATP + Adenylylsulfate -> ADP + 3'_Phosphoadenylyl_sulfate		 (( (Vmax * B) ) / (( (Km_Q + B) ) + (( (B ^ 2) ) / K_IQ))) buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM NaClO3, 50 µM Diadenosine pentaphosphate, nuclease P1
PH: 8
Temperature: 30
uniprot:O43252 [adenylyl-sulfate kinase(Enzyme) wildtype PAPS synthetase isoform 1]

Adenylylsulfate + ATP -> 3'_Phosphoadenylyl_sulfate + ADP		 (( (Vmax * A) ) / (KmA + A)) buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM NaClO3, 50 µM Diadenosine pentaphosphate, nuclease P1
PH: 8
Temperature: 30
uniprot:O43252 [adenylyl-sulfate kinase(Enzyme) wildtype PAPS synthetase isoform 1]

Adenylylsulfate + ATP -> ADP + 3'_Phosphoadenylyl_sulfate		 (( (( (Vmax * A) ) * Q) ) / (( (( (( (( (Kia * KmQ) ) + (KmA * Q)) ) + (KmQ * A)) ) + (A * Q)) ) + (( (A * (Q ^ 2)) ) / K_IQ))) buffer: 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT
PH: 8
Temperature: 30

Pathways

pathway id name
BioCyc:META_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:META_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:ECO_PWY-5340 sulfate activation for sulfonation
BioCyc:YEAST_PWY-5340 sulfate activation for sulfonation
BioCyc:ARA_PWY-5340 sulfate activation for sulfonation
BioCyc:VCHO_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:MTBH37RV_PWY-5345 superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:MTBH37RV_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:MTBCDC1551_PWY-6932 selenate reduction
BioCyc:MOUSE_PWY-5340 sulfate activation for sulfonation
BioCyc:BSUB_PWY-5340 sulfate activation for sulfonation
BioCyc:BSUB_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:ECOL316407_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOL316407_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BTHE_PWY-5345 superpathway of methionine biosynthesis (by sulfhydrylation)
BioCyc:META_PWY-5340 sulfate activation for sulfonation
BioCyc:META_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ECO_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:YEAST_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:MTBH37RV_PWY-5340 sulfate activation for sulfonation
BioCyc:MTBCDC1551_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL199310_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:MOUSE_PWY3DJ-35888 sulfur metabolism
BioCyc:BTHE_PWY-5340 sulfate activation for sulfonation
BioCyc:BTHE_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:META_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:ECO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:YEAST_PWY-821 superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:VCHO_PWY-5340 sulfate activation for sulfonation
BioCyc:VCHO_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:MTBCDC1551_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOO157_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOO157_PWY-5340 sulfate activation for sulfonation
BioCyc:BSUB_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:HUMAN_PWY-5340 sulfate activation for sulfonation
BioCyc:MTBH37RV_SO4ASSIM-PWY assimilatory sulfate reduction I
BioCyc:MTBCDC1551_PWY-5340 sulfate activation for sulfonation
BioCyc:ECOO157_SO4ASSIM-PWY sulfate reduction I (assimilatory)
BioCyc:ECOL199310_PWY-5340 sulfate activation for sulfonation
BioCyc:ECOL199310_SULFATE-CYS-PWY superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_PWY-5340 sulfate activation for sulfonation
PathBank:SMP0000821 Cysteine Biosynthesis
PathBank:SMP0000939 Sulfur Metabolism
PathBank:SMP0000940 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0000941 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0000942 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000943 Sulfur Metabolism (Isethionate)
PathBank:SMP0000944 Sulfur Metabolism (Methanesulfonate)
PlantCyc:ARA_PWY-5340 sulfate activation for sulfonation
PlantCyc:PLANT_PWY-5340 sulfate activation for sulfonation
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6802957 Oncogenic MAPK signaling
Reactome:R-MTU-870392 Mycobacterium tuberculosis biological processes
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-174362 Transport and metabolism of PAPS
Reactome:R-CEL-71387 Carbohydrate metabolism
Reactome:R-MTU-936635 Sulfate assimilation
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-1630316 Glycosaminoglycan metabolism
Reactome:R-HSA-5663202 Diseases of signal transduction by growth factor receptors and second messengers
Reactome:R-HSA-6802952 Signaling by BRAF and RAF1 fusions
Reactome:R-SCE-174362 Transport and metabolism of PAPS
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-1630316 Glycosaminoglycan metabolism
Reactome:R-CEL-174362 Transport and metabolism of PAPS
Reactome:R-MTU-936621 Sulfur compound metabolism
Reactome:R-SCE-1630316 Glycosaminoglycan metabolism
PathBank:SMP0121316 Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0121320 Sulfur Metabolism (Methanesulfonate)
PathBank:SMP0000041 Sulfate/Sulfite Metabolism
PathBank:SMP0000532 Sulfite Oxidase Deficiency
PathBank:SMP0002382 Sulfur Metabolism
PathBank:SMP0121315 Sulfur Metabolism
PathBank:SMP0121319 Sulfur Metabolism (Isethionate)
PathBank:SMP0063587 Selenoamino Acid Metabolism
PathBank:SMP0121317 Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0121282 Cysteine Biosynthesis
PathBank:SMP0121318 Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000029 Selenoamino Acid Metabolism