- 数据之源，洞见之始

EC: 2.5.1.47

cysteine synthase (O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase)

enzyme	lambda	experiment
uniprot:Q9YBL2 [cysteine synthase(Enzyme) wildtype] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * B) ) / (Km + B))`	buffer: 0.2 mM pyridoxal 5'-phosphate, 100 mM potassium phosphate, 1 mM EDTA PH: 6.7 Temperature: 60
uniprot:Q9YBL2 [cysteine synthase(Enzyme) wildtype] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * A) ) / (Km + A))`	buffer: 0.2 mM pyridoxal 5'-phosphate, 100 mM potassium phosphate, 1 mM EDTA PH: 6.7 Temperature: 60
uniprot:P0A1E3 [Thiocyanate] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (( (E * kcat) ) * A) ) / (KmA + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] 3_Chloro_L_alanine + 5_Mercapto_2_nitrobenzoate -> HCl + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> HCl + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (( (1 + (I / Kis)) ) + (( (( (I ^ 2) ) / Kis) ) * Kis2))) ) + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] Hydrogen_sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] Hydrogen_sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] Hydrogen_sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + Hydrogen_sulfide -> Acetate + L_Cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] Hydrogen_sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] Hydrogen_sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (E * kcat) ) * A) ) / (KmA + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + Hydrogen_sulfide -> Acetate + L_Cysteine	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [Thiocyanate] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] Hydrogen_sulfide + 3_Chloro_L_alanine -> L_Cysteine + HCl	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Thiocyanate] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> HCl + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (( (E * kcat) ) * A) ) * B) ) / (( (( (( (KmB * A) ) * (1 + (A / KiA))) ) + (( (KmA * B) ) * (1 + (B / KiB)))) ) + (A * B)))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Maleate] 5_Mercapto_2_nitrobenzoate + O_Acetyl_L_serine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Maleate] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Thiocyanate] 5_Mercapto_2_nitrobenzoate + O_Acetyl_L_serine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (KmA + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Thiocyanate] O_Acetyl_L_serine + 5_Mercapto_2_nitrobenzoate -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [Thiocyanate] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (KmA + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 5_Mercapto_2_nitrobenzoate + O_Acetyl_L_serine -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (( (1 + (I / Kis)) ) + (( (( (I ^ 2) ) / Kis) ) * Kis2))) ) + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 5_Mercapto_2_nitrobenzoate + O_Acetyl_L_serine -> Acetate + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 3_Chloro_L_alanine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P29848 [cysteine synthase(Enzyme) wildtype isozyme B] 3_Chloro_L_alanine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (KmA + (A * (1 + (I / Kii)))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (1 / Kis))) ) + A))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] 5_Mercapto_2_nitrobenzoate + 3_Chloro_L_alanine -> HCl + S_(3_Carboxy_4_nitrophenyl)_L_cysteine	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
uniprot:P0A1E3 [cysteine synthase(Enzyme) wildtype isozyme A] 3_Chloro_L_alanine + 5_Mercapto_2_nitrobenzoate -> S_(3_Carboxy_4_nitrophenyl)_L_cysteine + HCl	`(( (( (E * kcat) ) * A) ) / (( (KmA * (1 + (I / Kis))) ) + (A * (( (1 * I) ) / Kii))))`	buffer: 100 mM Hepes PH: 7 Temperature: 25
- [cysteine synthase(Enzyme) wildtype bound to recombinant Serine acetyltransferase (Arabidopsis thaliana)] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
uniprot:P16703 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
uniprot:P16703 [cysteine synthase(Enzyme) wildtype] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
- [cysteine synthase(Enzyme) wildtype bound to recombinant Serine acetyltransferase (Arabidopsis thaliana)] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
- [cysteine synthase(Enzyme) wildtype bound to recombinant Serine acetyltransferase (Arabidopsis thaliana)] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
- [cysteine synthase(Enzyme) wildtype bound to recombinant Serine acetyltransferase (Arabidopsis thaliana)] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
uniprot:P32260 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
uniprot:P32260 [cysteine synthase(Enzyme) wildtype] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (S ^ h)) ) / (1 + (( (S / Km) ) ^ h)))`	buffer: 50 mM Hepes/HCl, 1 mM dithiothreitol PH: 7.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] L_Cysteine + Cyanide -> 3_Cyano_L_alanine + Hydrogen_sulfide	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] Cyanide + L_Cysteine -> 3_Cyano_L_alanine + Hydrogen_sulfide	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] Cyanide + L_Cysteine -> 3_Cyano_L_alanine + Hydrogen_sulfide	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] O_Acetyl_L_serine + Cyanide -> Acetate + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] Cyanide + O_Acetyl_L_serine -> Acetate + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Cyanide -> 3_Cyano_L_alanine + Thiosulfate	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Cyanide -> 3_Cyano_L_alanine + Thiosulfate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Hydrogen_sulfide -> Thiosulfate + L_Cysteine	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Hydrogen_sulfide -> Thiosulfate + L_Cysteine	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] O_Acetyl_L_serine + Hydrogen_sulfide -> Acetate + L_Cysteine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] O_Acetyl_L_serine + Hydrogen_sulfide -> Acetate + L_Cysteine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] Cyanide + O_Acetyl_L_serine -> Acetate + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] O_Acetyl_L_serine + Cyanide -> Acetate + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] Cyanide + O_Acetyl_L_serine -> 3_Cyano_L_alanine + Acetate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] Hydrogen_sulfide + S_Sulfo_L_cysteine -> Thiosulfate + L_Cysteine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Hydrogen_sulfide -> Thiosulfate + L_Cysteine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Hydrogen_sulfide -> L_Cysteine + Thiosulfate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] Hydrogen_sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * (A ^ n)) ) / (( (A_half ^ n) ) + (A ^ n)))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] Cyanide + O_Acetyl_L_serine -> 3_Cyano_L_alanine + Acetate	`(( (( (E * kcat) ) * A) ) / (Km + A))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 7.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] L_Cysteine + Cyanide -> Hydrogen_sulfide + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] Cyanide + L_Cysteine -> 3_Cyano_L_alanine + Hydrogen_sulfide	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] Cyanide + L_Cysteine -> Hydrogen_sulfide + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mM DTT, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O01592 [Pyridoxal phosphate] S_Sulfo_L_cysteine + Cyanide -> Thiosulfate + 3_Cyano_L_alanine	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:O45679 [Pyridoxal phosphate] Cyanide + S_Sulfo_L_cysteine -> 3_Cyano_L_alanine + Thiosulfate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:Q93244 [Pyridoxal phosphate] Cyanide + S_Sulfo_L_cysteine -> 3_Cyano_L_alanine + Thiosulfate	`(( (( (E * kcat) ) * B) ) / (Km + B))`	buffer: 150 mM Tris-HCl, 1 mg/ml BSA PH: 8.5 Temperature: 25
uniprot:W5S3I4 [Pyridoxal phosphate] Hydrogen_sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris, 1 mM DTT PH: 7.5 Temperature: 36
uniprot:Q9S757 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * (A ^ h)) ) / (K + (A ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:Q9SXS7 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * (A ^ h)) ) / (K + (A ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:Q9S6Z7 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * (A ^ h)) ) / (K + (A ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:Q9S757 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * (B ^ h)) ) / (K + (B ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:Q9S6Z7 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * (B ^ h)) ) / (K + (B ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:Q9SXS7 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * (B ^ h)) ) / (K + (B ^ h)))`	buffer: Tris-HCl PH: 8 Temperature: 30
uniprot:P0ABK5 [cysteine synthase(Enzyme) wildtype bound to serine acetyltransferase] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (( (1 + (( (KiB * C) ) / (KiC * B))) ) + (C / KiC))) ) + (A * (1 + (KmB / B)))))`	buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.1 mM Pyridoxal 5-phosphate PH: 7.5 Temperature: 25
uniprot:P0ABK5 [cysteine synthase(Enzyme) wildtype] Hydrogen_sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (E * kcat) ) * A) ) / (( (KmA * (( (1 + (( (KiB * C) ) / (KiC * B))) ) + (C / KiC))) ) + (A * (1 + (KmB / B)))))`	buffer: 50 mM potassium phosphate, 1 mM EDTA, 0.1 mM Pyridoxal 5-phosphate PH: 7.5 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> L_Cysteine + Acetate	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] Sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P47998 [Pyridoxal phosphate] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (( (kcat * E) ) * S) ) / (Km + S))`	buffer: 100 mM 3-(N-morpholino)-2-hydroxypropanesulfonic acid PH: 7 Temperature: 25
uniprot:P32260 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 5 mM DTT PH: 7.5 Temperature: 25
uniprot:P32260 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Sulfide -> Acetate + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 50 mM Tris-HCl, 5 mM DTT PH: 7.5 Temperature: 25
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] Hydrogen_sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] Hydrogen_sulfide + O_Acetyl_L_serine -> Acetate + L_Cysteine	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] 3_Chloro_L_alanine + Hydrogen_sulfide -> HCl + L_Cysteine	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (Vmax * A) ) / (Km + A))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] O_Acetyl_L_serine + Hydrogen_sulfide -> Acetate + L_Cysteine	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] O_Acetyl_L_serine + Hydrogen_sulfide -> L_Cysteine + Acetate	`(( (Vmax * A) ) / (( (Km + (( (A ^ 2) ) / Ki)) ) + A))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] Hydrogen_sulfide + 3_Chloro_L_alanine -> L_Cysteine + HCl	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 0.1 M sodium phosphate, 10 mM dithiothreitol PH: 8 Temperature: 26
uniprot:P32260 [cysteine synthase(Enzyme) wildtype CysSynB] 3_Chloro_L_alanine + Hydrogen_sulfide -> HCl + L_Cysteine	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26
uniprot:Q00834 [cysteine synthase(Enzyme) wildtype CysSynA] 3_Chloro_L_alanine + Hydrogen_sulfide -> L_Cysteine + HCl	`(( (Vmax * (A ^ n)) ) / (k + (A ^ n)))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26
uniprot:Q43153 [cysteine synthase(Enzyme) wildtype] Hydrogen_sulfide + 3_Chloro_L_alanine -> HCl + L_Cysteine	`(( (Vmax * A) ) / (Km + A))`	buffer: 160 mM 2-amino 2-methyl 1-propanol, 10 mM dithiothreitol PH: 9.8 Temperature: 26

Pathways

pathway id	name
BioCyc:META_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:META_HSERMETANA-PWY	L-methionine biosynthesis III
BioCyc:META_PWY-5345	superpathway of L-methionine biosynthesis (by sulfhydrylation)
BioCyc:ARA_ASPSYNII-PWY	cyanide detoxification I
BioCyc:ARA_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:ARA_PWY-801	L-homocysteine and L-cysteine interconversion
BioCyc:MTBH37RV_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:MTBH37RV_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_CYSTSYN-PWY	cysteine biosynthesis I
BioCyc:META_PWY-7274	D-cycloserine biosynthesis
BioCyc:META_PWY-8010	L-cysteine biosynthesis IX (Trichomonas vaginalis)
BioCyc:META_PWY-6293	superpathway of L-cysteine biosynthesis (fungi)
BioCyc:META_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:META_PWY-821	superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:ECO_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:YEAST_HSERMETANA-PWY-1	L-methionine biosynthesis
BioCyc:ARA_HOMOCYSDEGR-PWY	L-cysteine biosynthesis III (from L-homocysteine)
BioCyc:MTBCDC1551_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:BSUB_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:SYNEL_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:SYNEL_PWY-6292	superpathway of L-cysteine biosynthesis (mammalian)
BioCyc:SYNEL_LCYSDEG-PWY	L-cysteine degradation II
BioCyc:SYNEL_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOL316407_LCYSDEG-PWY	L-cysteine degradation II
BioCyc:META_PWY-5344	L-homocysteine biosynthesis
BioCyc:META_PWY-I9	L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECO_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:YEAST_PWY-821	superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)
BioCyc:YEAST_PWY-5344	L-homocysteine biosynthesis
BioCyc:ARA_P401-PWY	cyanide degradation
BioCyc:HPY_PWY-I9	L-cysteine biosynthesis VI (from L-methionine)
BioCyc:ECOO157_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:ECOO157_CYSTSYN-PWY	cysteine biosynthesis I
BioCyc:BSUB_SULFATE-CYS-PWY	superpathway of sulfate assimilation and cysteine biosynthesis
BioCyc:SYNEL_HOMOCYSDEGR-PWY	L-cysteine biosynthesis III (from L-homocysteine)
BioCyc:YEAST_PWY-6293	cysteine biosynthesis IV (fungi)
BioCyc:MTBCDC1551_CYSTSYN-PWY	L-cysteine biosynthesis I
BioCyc:HPY_CYSTSYN-PWY	L-cysteine biosynthesis I
PathBank:SMP0000821	Cysteine Biosynthesis
PathBank:SMP0000939	Sulfur Metabolism
PathBank:SMP0000940	Sulfur Metabolism (Butanesulfonate)
PathBank:SMP0000941	Sulfur Metabolism (Propanesulfonate)
PathBank:SMP0000942	Sulfur Metabolism (Ethanesulfonate)
PathBank:SMP0000943	Sulfur Metabolism (Isethionate)
PathBank:SMP0000944	Sulfur Metabolism (Methanesulfonate)
PathBank:SMP0000993	Secondary Metabolites: Cysteine Biosynthesis from Serine
PlantCyc:ARA_P401-PWY	cyanide degradation
PlantCyc:PLANT_P401-PWY	cyanide degradation
PlantCyc:ARA_ASPSYNII-PWY	cyanide detoxification I
PlantCyc:ARA_CYSTSYN-PWY	L-cysteine biosynthesis I
Reactome:R-MTU-870392	Mycobacterium tuberculosis biological processes
Reactome:R-MTU-937250	Sulfur amino acid metabolism
Reactome:R-MTU-936721	Cysteine synthesis from O-acetylserine
Reactome:R-MTU-936621	Sulfur compound metabolism
PathBank:SMP0002382	Sulfur Metabolism
PathBank:SMP0087466	Glycine and Serine Metabolism
PathBank:SMP0087437	Homocysteine Degradation
PathBank:SMP0002309	Cysteine Metabolism
PathBank:SMP0002310	Methionine Metabolism and Salvage
PathBank:SMP0087423	Selenoamino Acid Metabolism
PathBank:SMP0087483	Methionine Metabolism

数据之源，洞见之始

Core Resources

Data & Analytics

Sophia Virtual Cell Workshop 2026

EC: 2.5.1.47

Pathways