EC: 2.4.1.1

glycogen phosphorylase ((1->4)-alpha-D-glucan:phosphate alpha-D-glucosyltransferase)

enzyme lambda metabolic reaction experiment
uniprot:B5AMJ8 [phosphorylase(Enzyme) wildtype]

alpha_D_Glucose_1_phosphate + Amylopectin -> Amylopectin + Phosphate		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM 2-(N-morpholino)- ethanesulfonic acid
PH: 6
Temperature: 37
uniprot:B5AMJ8 [phosphorylase(Enzyme) wildtype]

D_Glucose_1_phosphate + Glycogen -> Phosphate + Glycogen		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM 2-(N-morpholino)- ethanesulfonic acid
PH: 6
Temperature: 37
uniprot:P00490 [phosphorylase(Enzyme) mutant Y538F]

Maltoheptaose + Phosphate -> Maltohexaose + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * G) ) / (( (Km + G) ) + (( (G ^ 2) ) / Ki))) buffer: -
PH: 7
Temperature: 30
uniprot:P00490 [phosphorylase(Enzyme) wildtype]

Phosphate + Maltoheptaose -> alpha_D_Glucose_1_phosphate + Maltohexaose		 (( (( (E * kcat) ) * G) ) / (( (Km + G) ) + (( (G ^ 2) ) / Ki))) buffer: -
PH: 7
Temperature: 30
uniprot:P00490 [phosphorylase(Enzyme) mutant E637D]

Maltoheptaose + Phosphate -> Maltohexaose + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * G) ) / (( (Km + G) ) + (( (G ^ 2) ) / Ki))) buffer: -
PH: 7
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334A]

Starch + Phosphate -> Amylose + D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) wildtype]

Phosphate + Starch -> Amylose + D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334Q]

Phosphate + Starch -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334N]

Phosphate + Starch -> Amylose + D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334A]

Phosphate + Maltopentaose -> Maltotetraose + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334N]

Maltopentaose + Phosphate -> Maltotetraose + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) wildtype]

Maltopentaose + Phosphate -> Maltotetraose + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334Q]

Phosphate + Glycogen -> alpha_D_Glucan + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) wildtype]

Glycogen + Phosphate -> alpha_D_Glucose_1_phosphate + alpha_D_Glucan		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334N]

Phosphate + Glycogen -> alpha_D_Glucose_1_phosphate + alpha_D_Glucan		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334A]

Glycogen + Phosphate -> alpha_D_Glucan + alpha_D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [D-Glucono-1,5-lactone]

Phosphate + Starch -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / K_ic))) ) + (S * (1 + (I / K_iuc))))) buffer: 10 mM sodium acetate, 10 mM potassium sulfate, 50 mM Phosphate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [D-Glucono-1,5-lactone]

Starch + Phosphate -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / K_ic))) ) + (S * (1 + (I / K_iuc))))) buffer: 10 mM sodium acetate, 10 mM potassium sulfate, 50 mM Phosphate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [D-Glucono-1,5-lactone]

Phosphate + Starch -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (( (Km * (1 + (I / K_ic))) ) + (S * (1 + (I / K_iuc))))) buffer: 10 mM sodium acetate, 10 mM potassium sulfate, 50 mM Phosphate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334Q]

Phosphate + Maltopentaose -> alpha_D_Glucose_1_phosphate + Maltotetraose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) wildtype]

Amylose + D_Glucose_1_phosphate -> Phosphate + Starch		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334A]

Starch + Phosphate -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334N]

Starch + Phosphate -> Amylose + D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) wildtype]

Starch + Phosphate -> Amylose + D_Glucose_1_phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334Q]

Starch + Phosphate -> D_Glucose_1_phosphate + Amylose		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334Q]

D_Glucose_1_phosphate + Amylose -> Phosphate + Starch		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334N]

Amylose + D_Glucose_1_phosphate -> Starch + Phosphate		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
uniprot:Q8KQ56 [phosphorylase(Enzyme) mutant H334A]

D_Glucose_1_phosphate + Amylose -> Phosphate + Starch		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 10 mM sodium acetate, 10 mM potassium sulfate
PH: 6
Temperature: 30
- [phosphorylase(Enzyme) wildtype]

Phosphate + (1,4_alpha_D_Glucosyl)n -> (1,4_alpha_D_Glucosyl)n + D_Glucose_1_phosphate		 (( (V * S) ) / (Km + S)) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38
- [phosphorylase(Enzyme) wildtype]

D_Glucose_1_phosphate + (1,4_alpha_D_Glucosyl)n -> (1,4_alpha_D_Glucosyl)n + Phosphate		 (( (V * S) ) / (Km + S)) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38
- [phosphorylase(Enzyme) wildtype]

Phosphate + (1,4_alpha_D_Glucosyl)n -> (1,4_alpha_D_Glucosyl)n + D_Glucose_1_phosphate		 (( (( (( (Vf * S) ) / Kms) ) - (( (Vr * P) ) / Kmp)) ) / (( (1 + (S / Kms)) ) + (P / Kmp))) buffer: 10 mM K2HPO4/KH2PO4, 20 mM imidazole-HCl, 150 mM KCl, 5 mM MgCl2
PH: 7.2
Temperature: 38

Pathways

pathway id name
BioCyc:META_PWY-5941 glycogen degradation II
BioCyc:YEAST_PWY-5941 glycogen degradation II
BioCyc:YEAST_PWY-6981 chitin biosynthesis
BioCyc:MTBCDC1551_PWY-5941 glycogen degradation II
BioCyc:MTBCDC1551_GLYCOCAT-PWY glycogen degradation I
BioCyc:META_GLYCOCAT-PWY glycogen degradation I
BioCyc:ECO_GLYCOCAT-PWY glycogen degradation I
BioCyc:MOUSE_PWY-5767 glycogen degradation III
BioCyc:MOUSE_PWY-5941 glycogen degradation II
BioCyc:BSUB_GLYCOCAT-PWY glycogen degradation I
BioCyc:HUMAN_PWY-5941 glycogenolysis
BioCyc:MOUSE_PWY-842 starch degradation
BioCyc:ECOL316407_GLYCOCAT-PWY glycogen degradation I
BioCyc:ARA_PWY-7238 sucrose biosynthesis II
BioCyc:MTBCDC1551_PWY-6737 starch degradation V
PathBank:SMP0000958 Starch and Sucrose Metabolism
PathBank:SMP0000984 Secondary Metabolites: Trehalose Biosynthesis and Metabolism
PlantCyc:PLANT_PWY-7238 sucrose biosynthesis II
PlantCyc:ARA_PWY-7238 sucrose biosynthesis II
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-DME-71387 Carbohydrate metabolism
Reactome:R-HSA-8982491 Glycogen metabolism
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-DME-1430728 Metabolism
Reactome:R-DME-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-RNO-8982491 Glycogen metabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-DME-8982491 Glycogen metabolism
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-SCE-8982491 Glycogen metabolism
Reactome:R-BTA-8982491 Glycogen metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-70221 Glycogen breakdown (glycogenolysis)
Reactome:R-MMU-8982491 Glycogen metabolism
Reactome:R-RNO-71387 Carbohydrate metabolism
WikiPathways:WP500 Glycogen synthesis and degradation
PathBank:SMP0120838 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0000552 Glycogen Synthetase Deficiency
PathBank:SMP0000556 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0120837 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease
PathBank:SMP0000555 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0087345 Starch and Sucrose Metabolism
PathBank:SMP0120836 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0120840 Sucrase-Isomaltase Deficiency
PathBank:SMP0000553 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0000557 Sucrase-Isomaltase Deficiency
PathBank:SMP0087252 Starch and Sucrose Metabolism
PathBank:SMP0120835 Glycogen Synthetase Deficiency
PathBank:SMP0120839 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0000058 Starch and Sucrose Metabolism
PathBank:SMP0000554 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease