EC: 2.2.1.1

transketolase (sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glycolaldehydetransferase)

enzyme lambda metabolic reaction experiment
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> D_Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate -> D_Ribose_5_phosphate + D_Xylulose_5_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + D_Erythrose_4_phosphate -> D_Fructose_6_phosphate + D_Glyceraldehyde_3_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADP, Phosphoglucose isomerase, Glucose 6-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> D_Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate -> D_Erythrose_4_phosphate + D_Fructose_6_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADP, Phosphoglucose isomerase, Glucose 6-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + Formaldehyde -> D_Glyceraldehyde_3_phosphate + Dihydroxyacetone		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

Sedoheptulose_7_phosphate + 3_Hydroxypyruvate -> D_Erythrose + D_Fructose_6_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Sorbitol dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Fructose_6_phosphate + Glycolaldehyde -> D_Glyceraldehyde_3_phosphate + D_Xylulose		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + D_Erythrose -> D_Fructose + D_Glyceraldehyde_3_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADP, Phosphoglucose isomerase, Glucose 6-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Sedoheptulose_7_phosphate + Glyceraldehyde -> D_Ribose_5_phosphate + Xylulose		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Sorbitol dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + D_Ribose -> D_Sedoheptulose + D_Glyceraldehyde_3_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

Acetaldehyde + D_Xylulose_5_phosphate -> D_Glyceraldehyde_3_phosphate + D_Erythrose		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:P27302 [transketolase(Enzyme) wildtype]

Glycolaldehyde + D_Fructose_6_phosphate -> D_Xylulose + D_Glyceraldehyde_3_phosphate		 (( (V * S) ) / (Km + S)) buffer: 50 mM glycylglycine, 5 mM MgCl2, 1 mM thiamine diphosphate, NADH, Triosephosphate isomerase, Glycerol 3-phosphate dehydrogenase
PH: 8.5
Temperature: 30
uniprot:O20250 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + D_Ribose_5_phosphate -> Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate		 (( (( (kcat * E) ) * A) ) / (KmA + A)) buffer: 170 mM Tris/HCl, 5 mM MgCl2, 0.5 mM thiamine diphosphate, 0.5 mM Na2HAsO4, 0.3 mM NAD+, 3 mM cysteine, 1 U glyceraldehyde-3-phosphate dehydrogenase
PH: 7.5
Temperature: 36
uniprot:O20250 [transketolase(Enzyme) wildtype]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate		 (( (( (kcat * E) ) * B) ) / (KmB + B)) buffer: 170 mM Tris/HCl, 5 mM MgCl2, 0.5 mM thiamine diphosphate, 0.5 mM Na2HAsO4, 0.3 mM NAD+, 3 mM cysteine, 1 U glyceraldehyde-3-phosphate dehydrogenase
PH: 7.5
Temperature: 36
uniprot:P50137 [transketolase(Enzyme) wildtype]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> D_Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM TEA-DEA, 5 mM MgCl2, 0.1 mM ThPP, 0.15 mM NADH, 1 unit of alpha-Glycerol-3-P dehydrogenase, 1 unit of Triose phosphate isomerase
PH: 7.6
Temperature: 22
uniprot:P50137 [transketolase(Enzyme) wildtype]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> D_Glyceraldehyde_3_phosphate + D_Sedoheptulose_7_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 100 mM TEA-DEA, 5 mM MgCl2, 0.1 mM ThPP, 0.15 mM NADH, 1 unit of alpha-Glycerol-3-P dehydrogenase, 1 unit of Triose phosphate isomerase
PH: 7.6
Temperature: 22
- [Thiamine diphosphate]

D_Xylulose_5_phosphate + D_Ribose_5_phosphate -> Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: 200 mM HEPES-NaOH, 2 mM MgSO4, 0.1% BME
PH: 7.8
Temperature: 25
uniprot:P29401 [transketolase(Enzyme) wildtype]

D_Xylulose_5_phosphate + D_Ribose_5_phosphate -> D_Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 0.01 M Tris-HCl, 12.5 µM MgCl2, 0,05% Thiamin pyrophosphate
PH: 7.6
Temperature: 37
uniprot:P29401 [Thiamine diphosphate]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * C) ) / (Km_C + C)) buffer: 0.12 mM NADH, 2 mM MgCl2, 25 mM Tris, Triosephosphate isomerase, alpha-Glycerophosphate dehydrogenase
PH: 6.8
Temperature: 37
uniprot:P29401 [Thiamine diphosphate]

D_Xylulose_5_phosphate + D_Ribose_5_phosphate -> Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * A) ) / (Km_A + A)) buffer: 0.12 mM NADH, 2 mM MgCl2, 25 mM Tris, Triosephosphate isomerase, alpha-Glycerophosphate dehydrogenase
PH: 6.8
Temperature: 37
uniprot:P29401 [Thiamine diphosphate]

D_Ribose_5_phosphate + D_Xylulose_5_phosphate -> Sedoheptulose_7_phosphate + D_Glyceraldehyde_3_phosphate		 (( (Vmax * B) ) / (Km_B + B)) buffer: 0.12 mM NADH, 2 mM MgCl2, 25 mM Tris, Triosephosphate isomerase, alpha-Glycerophosphate dehydrogenase
PH: 6.8
Temperature: 37

Pathways

pathway id name
BioCyc:ECO_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ARA_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ARA_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
BioCyc:MOUSE_DRIBOPMET-PWY (deoxy)ribose phosphate degradation
BioCyc:MOUSE_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:BSUB_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:HUMAN_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ECO_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:YEAST_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:BSUB_PWY-1861 formaldehyde assimilation II (assimilatory RuMP Cycle)
BioCyc:ECOL316407_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:META_PWY-7218 photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
BioCyc:META_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:MTBH37RV_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:MTBH37RV_PENTOSE-P-PWY pentose phosphate pathway
BioCyc:ECOL316407_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:META_P21-PWY pentose phosphate pathway (partial)
BioCyc:META_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:YEAST_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:ARA_PWY-5723 Rubisco shunt
BioCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
BioCyc:MOUSE_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
BioCyc:BSUB_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
PlantCyc:ARA_PENTOSE-P-PWY pentose phosphate pathway
PlantCyc:ARA_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
PlantCyc:PLANT_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PWY-5723 Rubisco shunt
PlantCyc:PLANT_NONOXIPENT-PWY pentose phosphate pathway (non-oxidative branch)
PlantCyc:PLANT_PWY-5723 Rubisco shunt
PlantCyc:ARA_CALVIN-PWY Calvin-Benson-Bassham cycle
PlantCyc:ARA_PHOTOALL-PWY oxygenic photosynthesis
PlantCyc:PLANT_PENTOSE-P-PWY pentose phosphate pathway
Reactome:R-HSA-9818028 NFE2L2 regulates pentose phosphate pathway genes
Reactome:R-HSA-9755511 KEAP1-NFE2L2 pathway
Reactome:R-HSA-9711123 Cellular response to chemical stress
Reactome:R-HSA-9759194 Nuclear events mediated by NFE2L2
Reactome:R-HSA-163754 Insulin effects increased synthesis of Xylulose-5-Phosphate
Reactome:R-MMU-1430728 Metabolism
Reactome:R-RNO-163685 Integration of energy metabolism
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-2262752 Cellular responses to stress
Reactome:R-MMU-163685 Integration of energy metabolism
Reactome:R-RNO-71336 Pentose phosphate pathway
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-163754 Insulin effects increased synthesis of Xylulose-5-Phosphate
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-163754 Insulin effects increased synthesis of Xylulose-5-Phosphate
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-71336 Pentose phosphate pathway
Reactome:R-HSA-163685 Integration of energy metabolism
Reactome:R-HSA-8953897 Cellular responses to stimuli
Reactome:R-MMU-71336 Pentose phosphate pathway
Reactome:R-RNO-71387 Carbohydrate metabolism
PathBank:SMP0120802 Glucose-6-phosphate Dehydrogenase Deficiency
PathBank:SMP0120585 Transaldolase Deficiency
PathBank:SMP0000520 Transaldolase Deficiency
PathBank:SMP0002406 Riboneogenesis
PathBank:SMP0087246 Pentose Phosphate Pathway
PathBank:SMP0087270 Warburg Effect
PathBank:SMP0012094 Calvin-Benson Cycle
PathBank:SMP0063588 Pentose Phosphate Pathway
PathBank:SMP0120584 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0000519 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0002345 Xylitol Degradation
PathBank:SMP0120804 Transaldolase Deficiency
PathBank:SMP0000031 Pentose Phosphate Pathway
PathBank:SMP0000654 Warburg Effect
PathBank:SMP0012089 Photosynthesis
PathBank:SMP0120803 Ribose-5-phosphate Isomerase Deficiency
PathBank:SMP0120583 Glucose-6-phosphate Dehydrogenase Deficiency
PathBank:SMP0000518 Glucose-6-phosphate Dehydrogenase Deficiency
PathBank:SMP0086930 Warburg Effect
PathBank:SMP0087339 Pentose Phosphate Pathway
PathBank:SMP0087363 Warburg Effect
PathBank:SMP0012443 Pentose Phosphate Pathway