EC: 1.4.3.21

primary-amine oxidase (primary-amine:oxygen oxidoreductase (deaminating))

enzyme lambda metabolic reaction experiment
- [primary-amine oxidase(Enzyme) wildtype]

H2O + O2 + Tyramine -> NH3 + H2O2 + 4_Hydroxyphenylacetaldehyde		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
- [primary-amine oxidase(Enzyme) wildtype]

H2O + Benzylamine + O2 -> Benzaldehyde + NH3 + H2O2		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
- [primary-amine oxidase(Enzyme) wildtype]

O2 + H2O + 2_Aminomethylpyridine -> H2O2 + Pyridine_2_aldehyde + NH3		 (( (V * substrate) ) / (Km + substrate)) buffer: 0.1 M potassium phosphate, at 120 mM ionic strength
PH: 7.2
Temperature: 25
uniprot:Q43077 [primary-amine oxidase(Enzyme) wildtype]

H2O + Putrescine + O2 -> H2O2 + NH3 + 4_Aminobutanal		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
- [primary-amine oxidase(Enzyme) wildtype]

O2 + H2O + Putrescine -> NH3 + H2O2 + 4_Aminobutanal		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:Q43077 [primary-amine oxidase(Enzyme) wildtype]

O2 + H2O + Cadaverine -> H2O2 + 5_Aminopentanal + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
- [primary-amine oxidase(Enzyme) wildtype]

Cadaverine + O2 + H2O -> NH3 + H2O2 + 5_Aminopentanal		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:Q43077 [primary-amine oxidase(Enzyme) wildtype]

N_Methylputrescine + H2O + O2 -> H2O2 + NH3 + 4_Methylaminobutanal		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
- [primary-amine oxidase(Enzyme) wildtype]

H2O + O2 + N_Methylputrescine -> NH3 + 4_Methylaminobutanal + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:A4GZ88 [primary-amine oxidase(Enzyme) wildtype TRX-His6-MPO1]

1,3_Diaminopropane + H2O + O2 -> NH3 + 3_Aminopropanal + H2O2		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:A4GZ88 [primary-amine oxidase(Enzyme) wildtype TRX-His6-MPO1]

Putrescine + O2 + H2O -> H2O2 + 4_Aminobutanal + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:A4GZ88 [primary-amine oxidase(Enzyme) wildtype TRX-His6-MPO1]

O2 + H2O + Cadaverine -> H2O2 + 5_Aminopentanal + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
uniprot:A4GZ88 [primary-amine oxidase(Enzyme) wildtype TRX-His6-MPO1]

N_Methylputrescine + O2 + H2O -> NH3 + H2O2 + 4_Methylaminobutanal		 (( (Vmax * S) ) / (Km + S)) buffer: 0.2 M phosphate, 1200 U/ml glutamate dehydrogenase, 285 mM 2-Oxoglutarate, 9.5 mM NADH
PH: 7.4
Temperature: 37
- [primary-amine oxidase(Enzyme) wildtype]

Benzylamine + H2O + O2 -> NH3 + Benzaldehyde + H2O2		 (( (Vmax * A) ) / (Km + A)) buffer: 66 mM Potassium phosphate
PH: 7.2
Temperature: 36
- [primary-amine oxidase(Enzyme) wildtype]

H2O + O2 + 4_(Aminomethyl)benzenesulfonamide -> H2O2 + NH3 + 4_Formylbenzenesulfonamide		 (( (Vmax * A) ) / (Km + A)) buffer: 66 mM Potassium phosphate
PH: 7.2
Temperature: 36
uniprot:O75106 [primary-amine oxidase(Enzyme) wildtype AOC2]

O2 + H2O + 2_Phenylethylamine -> NH3 + H2O2 + Phenylacetaldehyde		 (( (Vmax * A) ) / (Km + (( (1 + (A / Ki)) ) * A))) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) wildtype AOC3]

O2 + 2_Phenylethylamine + H2O -> H2O2 + NH3 + Phenylacetaldehyde		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:O75106 [primary-amine oxidase(Enzyme) wildtype AOC2]

Benzylamine + O2 + H2O -> Benzaldehyde + H2O2 + NH3		 (( (Vmax * A) ) / (Km + (( (1 + (A / Ki)) ) * A))) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) wildtype AOC3]

H2O + O2 + Methylamine -> H2O2 + Formaldehyde + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:O75106 [primary-amine oxidase(Enzyme) wildtype AOC2]

H2O + O2 + Methylamine -> H2O2 + Formaldehyde + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:O75106 [primary-amine oxidase(Enzyme) wildtype AOC2]

Tyramine + H2O + O2 -> NH3 + H2O2 + 4_Hydroxyphenylacetaldehyde		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) mutant AOC3 M211V-Y394N-L469G]

2_Phenylethylamine + O2 + H2O -> H2O2 + Phenylacetaldehyde + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) wildtype AOC3]

H2O + 2_Phenylethylamine + O2 -> H2O2 + Phenylacetaldehyde + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:O75106 [primary-amine oxidase(Enzyme) wildtype AOC2]

H2O + O2 + Tryptamine -> Indole_3_acetaldehyde + H2O2 + NH3		 (( (Vmax * A) ) / (Km + (( (1 + (A / Ki)) ) * A))) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) mutant AOC3 M211V-Y394N-L469G]

O2 + Methylamine + H2O -> H2O2 + Formaldehyde + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37
uniprot:Q16853 [primary-amine oxidase(Enzyme) wildtype AOC3]

H2O + O2 + Methylamine -> Formaldehyde + H2O2 + NH3		 (( (Vmax * A) ) / (Km + A)) buffer: Krebs-Ringer phosphate glucose
PH: 7
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-6307 tryptophan degradation via tryptamine
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:HUMAN_PWY-6402 superpathway of melatonin degradation
BioCyc:META_PWY-6307 L-tryptophan degradation X (mammalian, via tryptamine)
BioCyc:META_PWY6666-2 dopamine degradation
BioCyc:META_PWY-6313 serotonin degradation
BioCyc:META_PWY-6342 noradrenaline and adrenaline degradation
BioCyc:META_THRDLCTCAT-PWY L-threonine degradation III (to methylglyoxal)
BioCyc:META_PWY-6071 superpathway of phenylethylamine degradation
BioCyc:ECO_2PHENDEG-PWY phenylethylamine degradation I
BioCyc:MOUSE_THRDLCTCAT-PWY threonine degradation III (to methylglyoxal)
BioCyc:META_2PHENDEG-PWY phenylethylamine degradation I
BioCyc:META_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:ECO_THREOCAT-PWY superpathway of L-threonine metabolism
BioCyc:ECO_THRDLCTCAT-PWY L-threonine degradation III (to methylglyoxal)
BioCyc:ECOL316407_THRDLCTCAT-PWY threonine degradation III (to methylglyoxal)
BioCyc:HUMAN_PWY-6117 spermine and spermidine degradation I
BioCyc:HUMAN_PWY-6313 serotonin degradation
BioCyc:META_PWY-0 putrescine degradation III
BioCyc:ECO_PWY-6071 superpathway of phenylethylamine degradation
BioCyc:MOUSE_PWY6666-2 dopamine degradation
BioCyc:ECOL316407_2PHENDEG-PWY phenylethylamine degradation I
BioCyc:HUMAN_PWY6666-2 dopamine degradation
BioCyc:HUMAN_PWY-0 putrescine degradation III
BioCyc:HUMAN_PWY-6342 noradrenaline and adrenaline degradation
BioCyc:HUMAN_2PHENDEG-PWY phenylethylamine degradation I
BioCyc:HUMAN_PWY-6399 melatonin degradation II
BioCyc:META_PWY-6318 L-phenylalanine degradation IV (mammalian, via side chain)
BioCyc:META_PWY-2 putrescine degradation IV
BioCyc:MOUSE_PWY-0 putrescine degradation III
BioCyc:MOUSE_PWY-3981 β-alanine biosynthesis I
BioCyc:ECOL316407_PWY-6071 superpathway of phenylethylamine degradation
BioCyc:ECOL316407_THREOCAT-PWY superpathway of threonine metabolism
PathBank:SMP0002041 Phenylethylamine Metabolism
PathBank:SMP0002118 L-Threonine Degradation to Methylglyoxal
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-211945 Phase I - Functionalization of compounds
Reactome:R-BTA-112310 Neurotransmitter release cycle
Reactome:R-BTA-379397 Enzymatic degradation of dopamine by COMT
Reactome:R-DRE-112315 Transmission across Chemical Synapses
Reactome:R-DRE-112311 Neurotransmitter clearance
Reactome:R-DRE-380615 Clearance of seratonin
Reactome:R-DRE-140179 Amine Oxidase reactions
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-112315 Transmission across Chemical Synapses
Reactome:R-HSA-112311 Neurotransmitter clearance
Reactome:R-HSA-380615 Clearance of seratonin
Reactome:R-HSA-1643685 Disease
Reactome:R-HSA-6785807 Interleukin-4 and Interleukin-13 signaling
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-181430 Norepinephrine Neurotransmitter Release Cycle
Reactome:R-MMU-379398 Enzymatic degradation of Dopamine by monoamine oxidase
Reactome:R-RNO-211945 Phase I - Functionalization of compounds
Reactome:R-RNO-112316 Neuronal System
Reactome:R-RNO-379401 Clearance of dopamine
Reactome:R-RNO-380612 Metabolism of serotonin
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-BTA-112316 Neuronal System
Reactome:R-BTA-379401 Clearance of dopamine
Reactome:R-BTA-380612 Metabolism of serotonin
Reactome:R-DRE-181430 Norepinephrine Neurotransmitter Release Cycle
Reactome:R-DRE-379398 Enzymatic degradation of Dopamine by monoamine oxidase
Reactome:R-DRE-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-141333 Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB
Reactome:R-HSA-181430 Norepinephrine Neurotransmitter Release Cycle
Reactome:R-HSA-379398 Enzymatic degradation of Dopamine by monoamine oxidase
Reactome:R-HSA-5579012 Defective MAOA causes BRUNS
Reactome:R-HSA-449147 Signaling by Interleukins
Reactome:R-MMU-141333 Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB
Reactome:R-MMU-112310 Neurotransmitter release cycle
Reactome:R-MMU-379397 Enzymatic degradation of dopamine by COMT
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-RNO-112315 Transmission across Chemical Synapses
Reactome:R-RNO-112311 Neurotransmitter clearance
Reactome:R-RNO-380615 Clearance of seratonin
Reactome:R-BTA-140179 Amine Oxidase reactions
Reactome:R-BTA-181430 Norepinephrine Neurotransmitter Release Cycle
Reactome:R-BTA-379398 Enzymatic degradation of Dopamine by monoamine oxidase
Reactome:R-DRE-112316 Neuronal System
Reactome:R-DRE-379401 Clearance of dopamine
Reactome:R-DRE-380612 Metabolism of serotonin
Reactome:R-DRE-1430728 Metabolism
Reactome:R-DRE-141333 Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB
Reactome:R-HSA-211945 Phase I - Functionalization of compounds
Reactome:R-HSA-112316 Neuronal System
Reactome:R-HSA-379401 Clearance of dopamine
Reactome:R-HSA-380612 Metabolism of serotonin
Reactome:R-HSA-5668914 Diseases of metabolism
Reactome:R-HSA-5579029 Metabolic disorders of biological oxidation enzymes
Reactome:R-HSA-168256 Immune System
Reactome:R-MMU-211945 Phase I - Functionalization of compounds
Reactome:R-MMU-112315 Transmission across Chemical Synapses
Reactome:R-MMU-112311 Neurotransmitter clearance
Reactome:R-MMU-380615 Clearance of seratonin
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-140179 Amine Oxidase reactions
Reactome:R-RNO-112310 Neurotransmitter release cycle
Reactome:R-RNO-379397 Enzymatic degradation of dopamine by COMT
Reactome:R-BTA-141333 Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB
Reactome:R-BTA-112315 Transmission across Chemical Synapses
Reactome:R-BTA-112311 Neurotransmitter clearance
Reactome:R-BTA-380615 Clearance of seratonin
Reactome:R-DRE-112310 Neurotransmitter release cycle
Reactome:R-DRE-379397 Enzymatic degradation of dopamine by COMT
Reactome:R-DRE-211859 Biological oxidations
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-140179 Amine Oxidase reactions
Reactome:R-HSA-112310 Neurotransmitter release cycle
Reactome:R-HSA-379397 Enzymatic degradation of dopamine by COMT
Reactome:R-HSA-1280215 Cytokine Signaling in Immune system
Reactome:R-MMU-140179 Amine Oxidase reactions
Reactome:R-MMU-112316 Neuronal System
Reactome:R-MMU-379401 Clearance of dopamine
Reactome:R-MMU-380612 Metabolism of serotonin
Reactome:R-RNO-141333 Biogenic amines are oxidatively deaminated to aldehydes by MAOA and MAOB
Reactome:R-RNO-181430 Norepinephrine Neurotransmitter Release Cycle
Reactome:R-RNO-379398 Enzymatic degradation of Dopamine by monoamine oxidase
WikiPathways:WP5298 Dravet syndrome: Scn1a-A1783V point mutation model
WikiPathways:WP4157 GABA metabolism (aka GHB)
WikiPathways:WP5372 Involvement of -secretase in neurodegenerative diseases
WikiPathways:WP4156 Biosynthesis and regeneration of tetrahydrobiopterin and catabolism of phenylalanine
PathBank:SMP0120540 Phenylketonuria
PathBank:SMP0000190 Hawkinsinuria
PathBank:SMP0000242 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000223 Non-Ketotic Hyperglycinemia
PathBank:SMP0000484 Dimethylglycine Dehydrogenase Deficiency
PathBank:SMP0000492 Ureidopropionase Deficiency
PathBank:SMP0120471 Cystathionine beta-Synthase Deficiency
PathBank:SMP0120487 Glycine N-Methyltransferase Deficiency
PathBank:SMP0120491 Hawkinsinuria
PathBank:SMP0120511 Tyrosinemia Type 3 (TYRO3)
PathBank:SMP0120519 Methionine Adenosyltransferase Deficiency
PathBank:SMP0120527 S-Adenosylhomocysteine (SAH) Hydrolase Deficiency
PathBank:SMP0000004 Glycine and Serine Metabolism
PathBank:SMP0000169 Alkaptonuria
PathBank:SMP0000429 Disulfiram Action Pathway
PathBank:SMP0000721 3-Phosphoglycerate Dehydrogenase Deficiency
PathBank:SMP0063474 Tropane, Piperidine, and Pyridine Alkaloid Biosynthesis
PathBank:SMP0063637 Methionine Metabolism
PathBank:SMP0063684 Tyrosine Metabolism
PathBank:SMP0120453 Alkaptonuria
PathBank:SMP0120461 Canavan Disease
PathBank:SMP0120493 Histidinemia
PathBank:SMP0120509 Tyrosinemia Type I
PathBank:SMP0120513 Tyrosinemia Type 2 (or Richner-Hanhart Syndrome)
PathBank:SMP0120598 Monoamine Oxidase-A Deficiency (MAO-A)
PathBank:SMP0120634 Homocystinuria-Megaloblastic Anemia Due to Defect in Cobalamin Metabolism, cblG Complementation Type
PathBank:SMP0000044 Histidine Metabolism
PathBank:SMP0000351 GABA-Transaminase Deficiency
PathBank:SMP0000191 Histidinemia
PathBank:SMP0000218 Tyrosinemia Type I
PathBank:SMP0000179 Dihydropyrimidine Dehydrogenase Deficiency (DHPD)
PathBank:SMP0000485 Hyperglycinemia, Non-Ketotic
PathBank:SMP0000493 Carnosinuria, Carnosinemia
PathBank:SMP0000533 Monoamine Oxidase-A Deficiency (MAO-A)
PathBank:SMP0063632 Histidine Metabolism
PathBank:SMP0120498 Hypermethioninemia
PathBank:SMP0120502 Hypoacetylaspartia
PathBank:SMP0120559 Tyrosinemia, Transient, of the Newborn
PathBank:SMP0120563 Dopamine beta-Hydroxylase Deficiency
PathBank:SMP0000007 beta-Alanine Metabolism
PathBank:SMP0000006 Tyrosine Metabolism
PathBank:SMP0000244 Sarcosinemia
PathBank:SMP0000424 Citalopram Action Pathway
PathBank:SMP0000494 Tyrosinemia, Transient, of the Newborn
PathBank:SMP0000498 Dopamine beta-Hydroxylase Deficiency
PathBank:SMP0000627 Citalopram Metabolism Pathway
PathBank:SMP0063471 Isoquinoline Alkaloid Biosynthesis
PathBank:SMP0063597 Aspartate Metabolism
PathBank:SMP0063649 Phenylalanine and Tyrosine Metabolism