L-threonine degradation III (to methylglyoxal)

Epperly BR, Dekker EE. Inactivation of Escherichia coli L-threonine dehydrogenase by 2,3-butanedione. Evidence for a catalytically essential arginine residue. J Biol Chem. 1989 Nov 05;264(31):18296–301. PMID: 2681195.; Boylan SA, Dekker EE. Growth, enzyme levels, and some metabolic properties of an Escherichia coli mutant grown on L-threonine as the sole carbon source. J Bacteriol. 1983 Oct;156(1):273–80. doi: 10.1128/jb.156.1.273-280.1983.; Boylan SA, Dekker EE. L-threonine dehydrogenase. Purification and properties of the homogeneous enzyme from Escherichia coli K-12. Journal of Biological Chemistry. 1981 Feb;256(4):1809–15. doi: 10.1016/s0021-9258(19)69880-7.; Komatsubara S, Murata K, Kisumi M, Chibata I. Threonine degradation by Serratia marcescens. J Bacteriol. 1978 Aug;135(2):318–23. doi: 10.1128/jb.135.2.318-323.1978.; Higgins IJ, Turner JM, Willetts AJ. Enzyme mechanism of aminoacetone metabolism by micro-organisms. Nature. 1967 Aug 19;215(5103):887–8. doi: 10.1038/215887a0. PMID: 4292865.

Metabolites

Beryllium

Formula: Be (9.012183)

CAS ID: 7440-41-7

Cadmium

Formula: Cd (113.903361)

CAS ID: 7440-43-9

CO2

Formula: CO2 (43.98983)

CAS ID: 124-38-9

Copper

Formula: Cu (62.929599)

CAS ID: 7440-50-8

Cyclohexane-1,2-dione

Formula: C6H8O2 (112.05242679999999)

CAS ID: 765-87-7

Diacetyl

Formula: C4H6O2 (86.0367776)

CAS ID: 431-03-8

Fe2+

Formula: Fe (55.934939)

CAS ID: 15438-31-0

H+

Formula: H (1.0078246)

CAS ID: 12408-02-5

H2O

Formula: H2O (18.0105642)

CAS ID: 7732-18-5

Hydrogen peroxide

Formula: H2O2 (34.0054792)

CAS ID: 7722-84-1

Manganese(2+)

Formula: Mn (54.938046)

CAS ID: 7439-96-5

Mercaptoethanol

Formula: C2H6OS (78.0139346)

CAS ID: 60-24-2

Mercury(2+)

Formula: Hg (201.970632)

CAS ID: 14302-87-5

Methylglyoxal

Formula: C3H4O2 (72.0211284)

CAS ID: 78-98-8

Nickel(2+)

Formula: Ni (57.935347)

CAS ID: 14701-22-5

Oxygen

Formula: O2 (31.98983)

CAS ID: 7782-44-7

Zinc cation

Formula: Zn (63.929145)

CAS ID: 23713-49-7

2,3-Pentanedione

Formula: C5H8O2 (100.05242679999999)

CAS ID: 600-14-6

Phenylglyoxal

Formula: C8H6O2 (134.0367776)

CAS ID: 1074-12-0

NAD(1-)

Formula: C21H26N7O14P2 (662.1012936000001)

CAS ID: 53-84-9



Enzyme

EC Number name full name note
1.1.1.1 alcohol dehydrogenase alcohol:NAD+ oxidoreductase
1.1.1.103 L-threonine 3-dehydrogenase L-threonine:NAD+ oxidoreductase
1.4.3.21 primary-amine oxidase primary-amine:oxygen oxidoreductase (deaminating)


Proteins

Protein ID name full name
P07913 tdh L-threonine 3-dehydrogenase
P37686 yiaY Probable alcohol dehydrogenase
P46883 tynA Primary amine oxidase