EC: 1.13.11.52

indoleamine 2,3-dioxygenase (D-tryptophan:oxygen 2,3-oxidoreductase (decyclizing))

enzyme lambda metabolic reaction experiment
uniprot:P47125 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDO His6-tagged]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 6.5
Temperature: 37
uniprot:P47125 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDO His6-tagged]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q2U3U2 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDOalpha His6-tagged]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q2UJE7 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDObeta His6-tagged]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 7.5
Temperature: 37
uniprot:Q2UJE7 [ indoleamine 2,3-dioxygenase(Enzyme) mutant IDObeta containing first intron (plus 17 aa), His6-tagged]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 7.5
Temperature: 37
uniprot:Q2U3U2 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDOalpha His6-tagged]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q2UJE7 [ indoleamine 2,3-dioxygenase(Enzyme) wildtype IDObeta His6-tagged]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 7.5
Temperature: 37
uniprot:Q2UJE7 [ indoleamine 2,3-dioxygenase(Enzyme) mutant IDObeta containing first intron (plus 17 aa), His6-tagged]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralized with NaOH), 10 µM methylene blue, 200 µg/ml (2,200 unit/ ml) catalase
PH: 7.5
Temperature: 37
uniprot:B3Y9H6 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:P28776 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:P28776 [Heme]

O2 + 5_Hydroxy_L_tryptophan -> 5_Hydroxy_N_formylkynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:B3Y9H8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:B3Y9H7 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:B3Y9H9 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q8R0V5 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:P28776 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (4000 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q8R0V5 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + 5_Hydroxy_L_tryptophan -> 5_Hydroxy_N_formylkynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:P28776 [Heme]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid (neutralised with NaOH), 200 µg/ml catalase, 10 µM methylene blue
PH: 6.5
Temperature: 37
uniprot:B3Y9H7 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
uniprot:A2Q0R5 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:A2Q0R5 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
uniprot:B3Y9H7 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:B3Y9H9 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
uniprot:B3Y9H9 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
uniprot:Q8R0V5 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 6.5
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (kcat * E) ) * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml (2200 U/ml) catalase
PH: 7.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * A) ) / (Km_A + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 100 U/ml catalase
PH: 6.5
Temperature: 37
uniprot:P14902 [1-Methyltryptophan]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * A) ) / (( (KmA * (1 + (I / Ki))) ) + A)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 100 U/ml catalase
PH: 6.5
Temperature: 37
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * A) ) / (KmA + (A * (1 + (I / Ki))))) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 100 U/ml catalase
PH: 6.5
Temperature: 37
uniprot:Q6ZQW0 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6F3I3 [Heme]

O2 + L_Tryptophan -> Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:C3Y9Y8 [Heme]

L_Tryptophan + O2 -> Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:A7SDW8 [Heme]

L_Tryptophan + O2 -> Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:A9UVU0 [Heme]

L_Tryptophan + O2 -> Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 μM methylene blue, 10 μg/ml of catalase (bovine liver), 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 μM methylene blue, 10 μg/ml of catalase (bovine liver), 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 μM methylene blue, 10 μg/ml of catalase (bovine liver), 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 6.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:P14902 [Heme]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 6.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

L_1_Methyltryptophan + O2 -> N_Formyl_methyl_L_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

5_Methoxy_DL_tryptophan + O2 -> 5_Methoxy_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

O2 + 5_Methyltryptophan -> 5_Methyl_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

O2 + 1_Methyl_D_tryptophan -> N_Formyl_methyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

5_Fluorotryptophan + O2 -> 5_Fluoro_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
uniprot:Q6ZQW0 [Heme]

O2 + 6_Methyltryptophan -> 6_Methyl_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 7.5
Temperature: 25
- [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 8
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 8
Temperature: 37
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ksi))))) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ksi))))) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ksi))))) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:P14902 [Heme]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_1_Methyltryptophan -> N_Formyl_methyl_L_kynurenine		 (( (( (E * kcat) ) * S) ) / (Km + (S * (1 + (S / Ksi))))) buffer: 100 mM Tris, 12 µM methylene blue, 100 µg/ml bovine liver catalase, 20 mM L-ascorbate
PH: 7.4
Temperature: 25
uniprot:A0A3B3IEZ6 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:A0A3B3IEZ6 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:H2LMG8 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:H2LMG8 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:P28776 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:P28776 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:Q8R0V5 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7
Temperature: 37
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + (B * (1 + (B / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + (B * (1 + (B / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + (B * (1 + (B / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + B)) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + 5_Hydroxy_L_tryptophan -> 5_Hydroxy_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

D_Tryptophan + O2 -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

5_Methyltryptophan + O2 -> 5_Methyl_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

O2 + 5_Fluorotryptophan -> 5_Fluoro_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 10 µM methylene blue, 10 µg/ml catalase, 20 mM L-ascorbate
PH: 8
Temperature: 25
uniprot:G1SJI2 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 0.025 mM methylene blue, 10 mM ascorrbic acid, 250 µg/ml catalase
PH: 8
Temperature: 24
uniprot:G1SJI2 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 0.025 mM methylene blue, 10 mM ascorrbic acid, 250 µg/ml catalase
PH: 7
Temperature: 24
uniprot:G1SJI2 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM potassium phosphate, 0.025 mM methylene blue, 10 mM ascorrbic acid, 250 µg/ml catalase
PH: 6
Temperature: 24
uniprot:G1SJI2 [Heme]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate
PH: 7.5
Temperature: 37
uniprot:B3Y9H6 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 8
Temperature: 37
uniprot:B3Y9H6 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 8
Temperature: 37
uniprot:B3Y9H7 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:H9GF95 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 6.5
Temperature: 37
uniprot:H9GF95 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 6.5
Temperature: 37
uniprot:A2BD60 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:A2BD60 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
- [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
- [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 20 mM ascorbic acid, 10 µM methylene blue, 200 µg/ml catalase
PH: 7.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (( (E * kcat) ) * A) ) * (1 + (A / Ksi_2))) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 3.6× MgCl2/Ca2+ free PBS, 20 mM ascorbic acid, 10 μM methylene blue, 800 nM catalase
PH: 7
Temperature: 22
uniprot:Q6ZQW0 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 3.6× MgCl2/Ca2+ free PBS, 20 mM ascorbic acid, 10 μM methylene blue, 800 nM catalase
PH: 7
Temperature: 22
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM ascorbic acid, 5 µM methylene blue, 10 µg/ml catalase (bovine)
PH: 6.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + L_1_Methyltryptophan -> N_Formyl_methyl_L_kynurenine		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 10 µg catalase, 20 mM sodium ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

O2 + 5_Hydroxy_L_tryptophan -> 5_Hydroxy_N_formylkynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM ascorbic acid, 5 µM methylene blue, 10 µg/ml catalase (bovine)
PH: 5.5
Temperature: 37
uniprot:P14902 [Heme]

O2 + D_Tryptophan -> N_Formyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM potassium phosphate, 10 mM ascorbic acid, 5 µM methylene blue, 10 µg/ml catalase (bovine)
PH: 7.5
Temperature: 37
uniprot:P14902 [Heme]

L_1_Methyltryptophan + O2 -> N_Formyl_methyl_L_kynurenine		 (( (( (kcat * E) ) * S) ) / (Km + S)) buffer: 50 mM Tris-HCl, 10 µM methylene blue, 10 µg catalase, 20 mM sodium ascorbate
PH: 8
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_1_Methyltryptophan -> N_Formyl_methyl_L_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 6.5
Temperature: 25
uniprot:P14902 [Heme]

1_Methyl_D_tryptophan + O2 -> N_Formyl_methyl_D_kynurenine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM KPi, 20 mM ascorbate, 10 µM methylene blue, 10 µg/ml catalase
PH: 6.5
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.05 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (1 + (A / Ki_eff1)) ) * (( (kcat * E) ) * A)) ) / (Km + (A * (1 + (A / Ki_eff))))) buffer: 0.1 M Tris-HCl, 20 mM ascorbate, 10 µM methylene blue, 30 µg/ml catalase
PH: 8
Temperature: 20
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * B) ) / (Km + (B * (1 + (B / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 150 µM beta-NADH, 1.0 µM cytochrome b5, 140 nM cytochrome b5 reductase, 54 nM copper-zinc superoxide dismutase, 12 nM catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

O2 + L_Tryptophan -> L_Formylkynurenine		 (( (( (( (E * kcat) ) * A) ) * (1 + (A / Ksi_2))) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 20 mM ascorbate, 10 µM methylene blue, 100 U/ml catalase
PH: 7
Temperature: 25
uniprot:P14902 [Heme]

L_Tryptophan + O2 -> L_Formylkynurenine		 (( (( (E * kcat) ) * A) ) / (Km + (A * (1 + (A / Ksi))))) buffer: 20 mM MOPS, 20 mM ascorbate, 10 µM methylene blue, 100 U/ml catalase
PH: 7
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation
BioCyc:HUMAN_PWY66-401 superpathway of tryptophan utilization
BioCyc:META_PWY-3502 superpathway of NAD biosynthesis in eukaryotes
BioCyc:META_PWY-5651 L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:YEAST_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:MOUSE_NADSYN-PWY NAD biosynthesis II (from tryptophan)
BioCyc:MOUSE_TRPCAT-PWY tryptophan degradation I (via anthranilate)
BioCyc:HUMAN_PWY-6309 L-kynurenine degradation
BioCyc:META_NADSYN-PWY NAD de novo biosynthesis II (from tryptophan)
BioCyc:YEAST_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_NADSYN-PWY NAD de novo biosynthesis
BioCyc:HUMAN_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
BioCyc:META_TRYPTOPHAN-DEGRADATION-1 L-tryptophan degradation III (eukaryotic)
BioCyc:META_PWY-6309 L-tryptophan degradation XI (mammalian, via kynurenine)
BioCyc:MOUSE_TRYPTOPHAN-DEGRADATION-1 tryptophan degradation III (eukaryotic)
BioCyc:MOUSE_PWY-5651 tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-71291 Amino acid and derivative metabolism
Reactome:R-HSA-71240 Tryptophan catabolism
Reactome:R-MMU-71240 Tryptophan catabolism
Reactome:R-RNO-71240 Tryptophan catabolism
Reactome:R-SCE-1430728 Metabolism
Reactome:R-HSA-71291 Amino acid and derivative metabolism
Reactome:R-MMU-71291 Amino acid and derivative metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-71291 Amino acid and derivative metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-SCE-71240 Tryptophan catabolism
WikiPathways:WP5078 T cell modulation in pancreatic cancer
WikiPathways:WP465 Tryptophan metabolism
WikiPathways:WP5044 Kynurenine pathway and links to cell senescence
PathBank:SMP0002353 Tryptophan Metabolism
PathBank:SMP0087330 Tryptophan Metabolism
PathBank:SMP0000063 Tryptophan Metabolism
PathBank:SMP0002338 NAD Metabolism