EC: 6.3.5.2

GMP synthase (glutamine-hydrolysing) (xanthosine-5'-phosphate:L-glutamine amido-ligase (AMP-forming))

enzyme lambda metabolic reaction experiment
uniprot:Q4V7C6 [Mg2+]

XMP + H2O + L_Glutamine + ATP -> AMP + L_Glutamate + Diphosphate + GMP		 (( (Vmax * C) ) / (Km + C)) buffer: 75 mM Tris-HCl
PH: 7.8
Temperature: 37
uniprot:Q4V7C6 [Mg2+]

ATP + L_Glutamine + XMP + H2O -> Diphosphate + GMP + L_Glutamate + AMP		 (( (Vmax * B) ) / (Km + B)) buffer: 75 mM Tris-HCl
PH: 7.8
Temperature: 37
uniprot:Q4V7C6 [Mg2+]

XMP + L_Glutamine + H2O + ATP -> L_Glutamate + AMP + Diphosphate + GMP		 (( (Vmax * A) ) / (Km + A)) buffer: 75 mM Tris-HCl
PH: 7.8
Temperature: 37
uniprot:Q4V7C6 [Mg2+]

NH3 + XMP + ATP -> AMP + GMP + Diphosphate		 (( (Vmax * C) ) / (Km + C)) buffer: 75 mM Tris-HCl
PH: 7.8
Temperature: 37
uniprot:Q4V7C6 [GMP synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + H2O + ATP + XMP -> Diphosphate + AMP + L_Glutamate + GMP		 (( (Vmax * C) ) / (Km + C)) buffer: 0.13 M Tris-HCl, 18 mM magnesium sulfate, 2.68 mM phosphoenolpyruvate, 8.9 U/ml pyruvate kinase (rabbit muscle)
PH: 7.6
Temperature: 37
uniprot:Q4V7C6 [GMP synthase (glutamine-hydrolysing)(Enzyme) wildtype]

L_Glutamine + XMP + H2O + ATP -> L_Glutamate + GMP + Diphosphate + AMP		 (( (Vmax * A) ) / (Km + A)) buffer: 0.13 M Tris-HCl, 18 mM magnesium sulfate, 2.68 mM phosphoenolpyruvate, 8.9 U/ml pyruvate kinase (rabbit muscle)
PH: 7.6
Temperature: 37
uniprot:Q4V7C6 [GMP synthase (glutamine-hydrolysing)(Enzyme) wildtype]

ATP + L_Glutamine + H2O + XMP -> GMP + Diphosphate + AMP + L_Glutamate		 (( (Vmax * B) ) / (Km + B)) buffer: 0.13 M Tris-HCl, 18 mM magnesium sulfate, 2.68 mM phosphoenolpyruvate, 8.9 U/ml pyruvate kinase (rabbit muscle)
PH: 7.6
Temperature: 37

Pathways

pathway id name
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:VCHO_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:VCHO_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:PLASMO_PWY-841 purine nucleotides de novo biosynthesis II
BioCyc:MTBH37RV_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:MTBH37RV_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:MTBCDC1551_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:MTBCDC1551_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:HPY_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-6125 superpathway of guanosine nucleotides de novo biosynthesis II
BioCyc:ANTHRA_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ECOL316407_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:CAULONA1000_PWY-841 purine nucleotides de novo biosynthesis II
BioCyc:PDIF272563_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:BTHE_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:CORYNE_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:GCF_000013425_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:HUMAN_PWY-7228 guanosine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:HPY_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:ANTHRA_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:PDIF272563_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:PDIF272563_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:CORYNE_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:GCF_000013425_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ECO_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:VCHO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:PLASMO_PWY-6121 5-aminoimidazole ribonucleotide biosynthesis I
BioCyc:PLASMO_PWY-6122 5-aminoimidazole ribonucleotide biosynthesis II
BioCyc:MTBH37RV_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:ECOO157_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ECOO157_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:CAULO_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:CAULO_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BTHE_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
BioCyc:CORYNE_GLUTAMINDEG-PWY L-glutamine degradation I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:ANTHRA_DENOVOPURINE2-PWY superpathway of purine nucleotides de novo biosynthesis II
BioCyc:AGRO_PWY-7221 guanosine ribonucleotides de novo biosynthesis
BioCyc:PDIF272563_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BTHE_GLUTAMINDEG-PWY glutamine degradation I
BioCyc:GCF_000013425_PWY-7228 superpathway of guanosine nucleotides de novo biosynthesis I
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
Reactome:R-RNO-9748784 Drug ADME
Reactome:R-SCE-9748784 Drug ADME
Reactome:R-SPO-9748787 Azathioprine ADME
Reactome:R-DDI-9748787 Azathioprine ADME
Reactome:R-HSA-9748787 Azathioprine ADME
Reactome:R-SPO-9748784 Drug ADME
Reactome:R-CEL-9748784 Drug ADME
Reactome:R-MMU-9748784 Drug ADME
Reactome:R-PFA-9748784 Drug ADME
Reactome:R-RNO-9748787 Azathioprine ADME
Reactome:R-SCE-9748787 Azathioprine ADME
Reactome:R-CEL-9748787 Azathioprine ADME
Reactome:R-DDI-9748784 Drug ADME
Reactome:R-HSA-9748784 Drug ADME
Reactome:R-MMU-9748787 Azathioprine ADME
Reactome:R-PFA-9748787 Azathioprine ADME
Reactome:R-DDI-8956320 Nucleotide biosynthesis
Reactome:R-HSA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-RNO-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-CEL-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-PFA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-RNO-8956320 Nucleotide biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-PFA-1430728 Metabolism
Reactome:R-PFA-15869 Nucleotide metabolism
Reactome:R-RNO-1430728 Metabolism
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SPO-8956320 Nucleotide biosynthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-8956320 Nucleotide biosynthesis
Reactome:R-DDI-1430728 Metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-PFA-8956320 Nucleotide biosynthesis
Reactome:R-RNO-15869 Nucleotide metabolism
Reactome:R-SCE-8956320 Nucleotide biosynthesis
Reactome:R-SPO-73817 Purine ribonucleoside monophosphate biosynthesis
PathBank:SMP0120666 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120670 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120714 Homocarnosinosis
PathBank:SMP0120850 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120496 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000339 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0000609 Mercaptopurine Metabolism Pathway
PathBank:SMP0087278 Glutamate Metabolism
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120669 Adenosine Deaminase Deficiency
PathBank:SMP0120673 AICA-Ribosiduria
PathBank:SMP0120709 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120717 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120797 Xanthinuria Type II
PathBank:SMP0120821 Myoadenylate Deaminase Deficiency
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0121307 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0122223 Purine Nucleotides De Novo Biosynthesis 2
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000385 Homocarnosinosis
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000136 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000567 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120716 Hyperinsulinism-Hyperammonemia Syndrome
PathBank:SMP0120728 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120752 Molybdenum Cofactor Deficiency
PathBank:SMP0120756 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120796 Xanthinuria Type I
PathBank:SMP0120820 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0120445 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0087332 Purine Metabolism
PathBank:SMP0063590 Glutamate Metabolism
PathBank:SMP0120659 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120819 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120438 2-Hydroxyglutric Aciduria (D and L Form)
PathBank:SMP0120494 Homocarnosinosis
PathBank:SMP0120631 Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000072 Glutamate Metabolism
PathBank:SMP0000243 4-Hydroxybutyric Aciduria/Succinic Semialdehyde Dehydrogenase Deficiency
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000427 Azathioprine Action Pathway