EC: 4.3.2.2

adenylosuccinate lyase (N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming))

enzyme lambda metabolic reaction experiment
uniprot:P30566 [Adenosine phosphonobutyrate diphosphate]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide + Fumarate		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 50 mM potassium phosphate, 150 mM KCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol
PH: 7
Temperature: 25
uniprot:P12047 [Adenosine phosphonobutyrate diphosphate]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide + Fumarate		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P30566 [adenylosuccinate lyase(Enzyme) wildtype]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 150 mM KCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) wildtype]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P30566 [Adenosine phosphonobutyrate diphosphate]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 50 mM potassium phosphate, 150 mM KCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol
PH: 7
Temperature: 25
uniprot:P12047 [Adenosine phosphonobutyrate diphosphate]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (Vmax * S) ) / (S + (Km * (1 + (I / Ki))))) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P30566 [adenylosuccinate lyase(Enzyme) wildtype]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM potassium phosphate, 150 mM KCl, 1 mM EDTA, 1 mM DTT, 10 % glycerol
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) wildtype]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) wildtype His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant M10L His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) wildtype His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant T367S His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant M10L His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant I123R His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant T367R His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant I123W His6-tagged]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant T367S His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant I123W His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant T367R His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide + Fumarate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant I123R His6-tagged]

1_(5'_Phosphoribosyl)_5_amino_4_(N_succinocarboxamide)_imidazole -> Fumarate + 1_(5'_Phosphoribosyl)_5_amino_4_imidazolecarboxamide		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM HEPES
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) wildtype]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141R]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141A + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [H+]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (V0 / (( (1 + (10 ^ (pK1 - pH))) ) + (10 ^ (pH - pK2)))) buffer: 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM HEPES
PH: 6
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141R + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141Q + mutant R67M:H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141Q + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141Q + mutant H68K]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141L + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141Q + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141Q + mutant H68A]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [adenylosuccinate lyase(Enzyme) mutant H141E + mutant H68Q]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 20 mM potassium phosphate, 20 mM NaCl
PH: 7
Temperature: 25
uniprot:P12047 [H+]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (V0 / (( (1 + (10 ^ (pK1 - pH))) ) + (10 ^ (pH - pK2)))) buffer: 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM HEPES
PH: 6
Temperature: 25
uniprot:P12047 [H+]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (V0 / (( (1 + (10 ^ (pK1 - pH))) ) + (10 ^ (pH - pK2)))) buffer: 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM HEPES
PH: 6
Temperature: 25
uniprot:P12047 [H+]

N6_(1,2_Dicarboxyethyl)_AMP -> Fumarate + AMP		 (V0 / (( (1 + (10 ^ (pK1 - pH))) ) + (10 ^ (pH - pK2)))) buffer: 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM HEPES
PH: 6
Temperature: 25
uniprot:P12047 [H+]

N6_(1,2_Dicarboxyethyl)_AMP -> AMP + Fumarate		 (V0 / (( (1 + (10 ^ (pK1 - pH))) ) + (10 ^ (pH - pK2)))) buffer: 50 mM 2-(N-morpholino)ethanesulfonic acid, 50 mM HEPES
PH: 6
Temperature: 25

Pathways

pathway id name
BioCyc:HUMAN_PWY-6124 inosine-5'-phosphate biosynthesis
BioCyc:META_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:META_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:ECO_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:YEAST_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:YEAST_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:ECOL199310_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:GCF_000013425_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:HUMAN_PWY66-409 superpathway of purine nucleotide salvage
BioCyc:META_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:HPY_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:ECOL199310_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:ECOL199310_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:ECOL316407_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:GCF_000013425_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:META_PWY-6148 tetrahydromethanopterin biosynthesis
BioCyc:ECO_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:YEAST_PWY-6126 adenosine nucleotides de novo biosynthesis
BioCyc:HPY_PWY-7229 superpathway of adenosine nucleotides de novo biosynthesis I
BioCyc:ECOL199310_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:ECOL199310_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:BSUB_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:BSUB_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:ECOL316407_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:HUMAN_PWY-841 purine nucleotides de novo biosynthesis
BioCyc:HUMAN_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:META_PWY-841 superpathway of purine nucleotides de novo biosynthesis I
BioCyc:META_PWY-7234 inosine-5'-phosphate biosynthesis III
BioCyc:META_PWY-6123 inosine-5'-phosphate biosynthesis I
BioCyc:MOUSE_PWY-6124 inosine-5'-phosphate biosynthesis II
BioCyc:BSUB_PWY-7219 adenosine ribonucleotides de novo biosynthesis
BioCyc:GCF_000013425_PWY-7219 adenosine ribonucleotides de novo biosynthesis
PathBank:SMP0000809 Aspartate Metabolism
PathBank:SMP0000927 Purine Nucleotides De Novo Biosynthesis
PathBank:SMP0002047 Purine Nucleotides De Novo Biosynthesis 2
Reactome:R-GGA-15869 Nucleotide metabolism
Reactome:R-GGA-1660598 Metabolism
Reactome:R-GGA-421203 De novo synthesis of AMP
Reactome:R-HSA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-CEL-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-GGA-419470 Nucleotide metabolism
Reactome:R-GGA-419140 De novo synthesis of IMP
Reactome:R-HSA-8956320 Nucleotide biosynthesis
Reactome:R-MMU-8956320 Nucleotide biosynthesis
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-CEL-15869 Nucleotide metabolism
Reactome:R-GGA-1430728 Metabolism
Reactome:R-GGA-8956320 Nucleotide biosynthesis
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SPO-8956320 Nucleotide biosynthesis
Reactome:R-CEL-1430728 Metabolism
Reactome:R-CEL-8956320 Nucleotide biosynthesis
Reactome:R-GGA-73817 Purine ribonucleoside monophosphate biosynthesis
Reactome:R-GGA-419474 Purine metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-SCE-8956320 Nucleotide biosynthesis
Reactome:R-SPO-73817 Purine ribonucleoside monophosphate biosynthesis
WikiPathways:WP4792 Purine metabolism
WikiPathways:WP4224 Purine metabolism and related disorders
PathBank:SMP0120448 Adenosine Deaminase Deficiency
PathBank:SMP0120452 AICA-Ribosiduria
PathBank:SMP0120508 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0120532 Molybdenum Cofactor Deficiency
PathBank:SMP0120536 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0120577 Xanthinuria Type I
PathBank:SMP0120601 Mitochondrial DNA Depletion Syndrome
PathBank:SMP0000167 Adenylosuccinate Lyase Deficiency
PathBank:SMP0000220 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0000512 Xanthinuria Type I
PathBank:SMP0000536 Mitochondrial DNA Depletion Syndrome-3
PathBank:SMP0087165 Aspartate Metabolism
PathBank:SMP0063668 Purine Metabolism
PathBank:SMP0120600 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0000050 Purine Metabolism
PathBank:SMP0000144 Adenosine Deaminase Deficiency
PathBank:SMP0000203 Molybdenum Cofactor Deficiency
PathBank:SMP0000428 Mercaptopurine Action Pathway
PathBank:SMP0000535 Adenine Phosphoribosyltransferase Deficiency (APRT)
PathBank:SMP0120449 Adenylosuccinate Lyase Deficiency
PathBank:SMP0120461 Canavan Disease
PathBank:SMP0120489 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0120497 Xanthine Dehydrogenase Deficiency (Xanthinuria)
PathBank:SMP0120578 Xanthinuria Type II
PathBank:SMP0120602 Myoadenylate Deaminase Deficiency
PathBank:SMP0000067 Aspartate Metabolism
PathBank:SMP0000365 Gout or Kelley-Seegmiller Syndrome
PathBank:SMP0000210 Purine Nucleoside Phosphorylase Deficiency
PathBank:SMP0000175 Canavan Disease
PathBank:SMP0000430 Thioguanine Action Pathway
PathBank:SMP0000513 Xanthinuria Type II
PathBank:SMP0000537 Myoadenylate Deaminase Deficiency
PathBank:SMP0120502 Hypoacetylaspartia
PathBank:SMP0000364 Lesch-Nyhan Syndrome (LNS)
PathBank:SMP0000168 AICA-Ribosiduria
PathBank:SMP0000192 Hypoacetylaspartia
PathBank:SMP0000427 Azathioprine Action Pathway
PathBank:SMP0087239 Purine Metabolism
PathBank:SMP0063597 Aspartate Metabolism