EC: 1.13.11.31

arachidonate 12-lipoxygenase (arachidonate:oxygen 12-oxidoreductase)

enzyme lambda metabolic reaction experiment
- [Fe2+]

O2 + Arachidonate -> H2O + Leukotriene_A4		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 25 mM Trizma
PH: 7.5
Temperature: 30
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype N-terminal His6 tagged]

O2 + Arachidonate -> 12_HPETE		 (( (Vmax * S) ) / (( (Km + S) ) + (( (S ^ n) ) / Ki))) buffer: 20 mM Hepes-NaOH
PH: 8
Temperature: 22
uniprot:P16050 [13-HPODE]

O2 + Arachidonate -> 12_HPETE		 (( (( (E * kcat) ) * S) ) / (Km + S)) buffer: 25 mM oxygen-saturated HEPES
PH: 7.5
Temperature: 25
- [Fe2+]

Arachidonate + O2 -> Leukotriene_A4 + H2O		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 25 mM Trizma
PH: 7.5
Temperature: 25
- [Fe2+]

Arachidonate + O2 -> H2O + Leukotriene_A4		 (( (( (kcat * E) ) * S) ) / (S + Km)) buffer: 25 mM Trizma
PH: 7.5
Temperature: 10
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype His-tagged]

O2 + Arachidonate -> 12_HPETE		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.006% Tween20
PH: 8
Temperature: 25
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype His-tagged]

Linoleate + O2 -> (9Z,11E)_(13S)_13_Hydroperoxyoctadeca_9,11_dienoate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM sodium phosphate, 0.006% Tween20
PH: 8
Temperature: 25
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype His-tagged]

Arachidonate + O2 -> 12_HPETE		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype His-tagged]

5,6_EET + O2 + H2O -> 5,6_Epoxy_12_hydroxyeicosatrienoic_acid		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37
uniprot:P18054 [arachidonate 12-lipoxygenase(Enzyme) wildtype His-tagged]

O2 + 2 Leukotriene_A4 + 2 H2O -> Lipoxin		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris/HCl
PH: 7.4
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY66-392 lipoxin biosynthesis
BioCyc:HUMAN_PWY66-397 resolvin D biosynthesis
BioCyc:META_PWY66-397 resolvin D biosynthesis
BioCyc:META_PWY66-392 lipoxin biosynthesis
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-556833 Metabolism of lipids
Reactome:R-BTA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-BTA-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-BTA-9025106 Biosynthesis of DPAn-6 SPMs
Reactome:R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-HSA-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
Reactome:R-HSA-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-HSA-9025106 Biosynthesis of DPAn-6 SPMs
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-MMU-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-MMU-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
Reactome:R-MMU-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-MMU-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-MMU-9026290 Biosynthesis of DPAn-3-derived maresins
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
Reactome:R-RNO-2142700 Biosynthesis of Lipoxins (LX)
Reactome:R-RNO-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
Reactome:R-SSC-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-SSC-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
Reactome:R-BTA-8978868 Fatty acid metabolism
Reactome:R-BTA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
Reactome:R-BTA-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-BTA-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-HSA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
Reactome:R-HSA-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-HSA-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-HSA-9026290 Biosynthesis of DPAn-3-derived maresins
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
Reactome:R-MMU-2142700 Biosynthesis of Lipoxins (LX)
Reactome:R-MMU-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-MMU-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
Reactome:R-RNO-2142753 Arachidonate metabolism
Reactome:R-RNO-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-RNO-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-RNO-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-RNO-9018681 Biosynthesis of protectins
Reactome:R-RNO-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-SSC-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-SSC-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-SSC-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-SSC-9018681 Biosynthesis of protectins
Reactome:R-SSC-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-BTA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-BTA-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-BTA-9018681 Biosynthesis of protectins
Reactome:R-BTA-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-HSA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-HSA-9018681 Biosynthesis of protectins
Reactome:R-HSA-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-MMU-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-MMU-9025106 Biosynthesis of DPAn-6 SPMs
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-RNO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
Reactome:R-RNO-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
Reactome:R-RNO-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-RNO-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-RNO-9026290 Biosynthesis of DPAn-3-derived maresins
Reactome:R-SSC-2142753 Arachidonate metabolism
Reactome:R-SSC-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-SSC-9018679 Biosynthesis of EPA-derived SPMs
Reactome:R-SSC-9018683 Biosynthesis of DPA-derived SPMs
Reactome:R-BTA-2142753 Arachidonate metabolism
Reactome:R-BTA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-BTA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-BTA-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-HSA-2142700 Biosynthesis of Lipoxins (LX)
Reactome:R-HSA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-HSA-9026286 Biosynthesis of DPAn-3-derived protectins and resolvins
Reactome:R-MMU-2142753 Arachidonate metabolism
Reactome:R-MMU-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
Reactome:R-MMU-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-MMU-9023661 Biosynthesis of E-series 18(R)-resolvins
Reactome:R-MMU-9018681 Biosynthesis of protectins
Reactome:R-MMU-9025094 Biosynthesis of DPAn-3 SPMs
Reactome:R-RNO-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-RNO-9025106 Biosynthesis of DPAn-6 SPMs
Reactome:R-SSC-8978868 Fatty acid metabolism
Reactome:R-SSC-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
Reactome:R-SSC-9018896 Biosynthesis of E-series 18(S)-resolvins
Reactome:R-SSC-9025106 Biosynthesis of DPAn-6 SPMs
WikiPathways:WP4586 Metabolism of alpha-linolenic acid
WikiPathways:WP4313 Ferroptosis
PathBank:SMP0120730 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0087315 Arachidonic Acid Metabolism