EC: 3.5.4.5

cytidine deaminase (cytidine/2'-deoxycytidine aminohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P32320 [1-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-4-hydroxy-1,3-diazinan-2-one]

H2O + 2'_Deoxycytidine -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [1-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-4-hydroxy-1,3-diazinan-2-one]

H2O + 2'_Deoxycytidine -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [1-[3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-4-hydroxy-1,3-diazinan-2-one]

H2O + 2'_Deoxycytidine -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68Q]

H2O + 2'_Deoxycytidine -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [6-[3,5(Cytidyl)acryloylamino] hexanoic acid]

H2O + 2'_Deoxycytidine -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant Y60G]

2'_Deoxycytidine + H2O -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [6-[3,5(Cytidyl)acryloylamino] hexanoic acid]

2'_Deoxycytidine + H2O -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [5-Fluorozebularine]

2'_Deoxycytidine + H2O -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [5-Fluorozebularine]

2'_Deoxycytidine + H2O -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) wildtype]

H2O + 2'_Deoxycytidine -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) wildtype]

2'_Deoxycytidine + H2O -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68Q]

H2O + 2'_Deoxycytidine -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant Y60G]

H2O + 2'_Deoxycytidine -> NH3 + 2'_Deoxyuridine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68G]

H2O + 2'_Deoxycytidine -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68G]

Cytarabine + H2O -> Arabinofuranosyluracil + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant Y60G]

Cytarabine + H2O -> NH3 + Arabinofuranosyluracil		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) wildtype]

H2O + Cytarabine -> Arabinofuranosyluracil + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68Q]

Cytarabine + H2O -> Arabinofuranosyluracil + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) wildtype]

H2O + Cytidine -> NH3 + Uridine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant Y60G]

Cytidine + H2O -> Uridine + NH3		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68Q]

H2O + Cytidine -> NH3 + Uridine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [cytidine deaminase(Enzyme) mutant R68G]

H2O + Cytidine -> NH3 + Uridine		 (( (Vmax * S) ) / (Km + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37
uniprot:P32320 [5-Fluorozebularine]

2'_Deoxycytidine + H2O -> 2'_Deoxyuridine + NH3		 (( (Vmax * S) ) / (( (Km * (1 + (I / Ki))) ) + S)) buffer: 100 mM Tris-HCl
PH: 7.5
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:META_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:ECO_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:ECO_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:ECO_PWY0-181 salvage pathways of pyrimidine deoxyribonucleotides
BioCyc:YEAST_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:MTBH37RV_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:MTBH37RV_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:MTBCDC1551_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:ECOO157_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:ECOL199310_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ECOL199310_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:MOUSE_PWY-6556 pyrimidine ribonucleosides degradation II
BioCyc:MOUSE_DRIBOPMET-PWY (deoxy)ribose phosphate degradation
BioCyc:ECOL316407_PWY0-181 salvage pathways of pyrimidine deoxyribonucleotides
BioCyc:HUMAN_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:HUMAN_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:META_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:META_PWY-6556 pyrimidine ribonucleosides salvage II
BioCyc:META_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:ECO_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:ECO_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
BioCyc:ARA_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:VCHO_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:MTBH37RV_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBCDC1551_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBCDC1551_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:ECOO157_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:ECOL199310_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:MOUSE_P1-PWY purine and pyrimidine metabolism
BioCyc:MOUSE_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:MOUSE_PWY0-181 salvage pathways of pyrimidine deoxyribonucleotides
BioCyc:BSUB_P1-PWY purine and pyrimidine metabolism
BioCyc:ECOL316407_PWY-6556 pyrimidine ribonucleosides salvage II
BioCyc:HUMAN_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:META_PWY-7209 superpathway of pyrimidine ribonucleosides degradation
BioCyc:ECO_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:ECO_PWY-6556 pyrimidine ribonucleosides salvage II
BioCyc:YEAST_PWY-6556 pyrimidine ribonucleosides degradation II
BioCyc:YEAST_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:ARA_PWY-6556 pyrimidine ribonucleosides salvage II
BioCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:VCHO_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:VCHO_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
BioCyc:MTBH37RV_PWY-6556 pyrimidine ribonucleosides salvage II
BioCyc:MTBH37RV_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:MTBCDC1551_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:ECOO157_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:ECOL199310_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:ECOL199310_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:ECOL316407_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:ECOL316407_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:ECOL316407_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:ECOL316407_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:HUMAN_PWY-7209 pyrimidine ribonucleosides degradation
BioCyc:HUMAN_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:META_PWY0-1295 pyrimidine ribonucleosides degradation
BioCyc:META_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:META_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:ECO_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:VCHO_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:VCHO_PWY-7199 pyrimidine deoxyribonucleosides salvage
BioCyc:VCHO_PWY-7193 pyrimidine ribonucleosides salvage I
BioCyc:ECOO157_PWY-7181 pyrimidine deoxyribonucleosides degradation
BioCyc:ECOL199310_PWY0-1298 superpathway of pyrimidine deoxyribonucleosides degradation
BioCyc:MOUSE_PWY0-1298 pyrimidine deoxyribonucleosides degradation
BioCyc:BSUB_PWY0-163 salvage pathways of pyrimidine ribonucleotides
BioCyc:BSUB_PWY0-1295 pyrimidine ribonucleosides degradation
PathBank:SMP0002038 Pyrimidine Ribonucleosides Degradation
PathBank:SMP0002073 Salvage Pathways of Pyrimidine Deoxyribonucleotides
PathBank:SMP0002075 Pyrimidine Deoxyribonucleosides Degradation
PlantCyc:ARA_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:PLANT_PWY-7193 pyrimidine ribonucleosides salvage I
PlantCyc:PLANT_PWY-6556 pyrimidine ribonucleosides salvage II
PlantCyc:PLANT_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:PLANT_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
PlantCyc:ARA_PWY-6556 pyrimidine ribonucleosides salvage II
PlantCyc:PLANT_PWY-7200 superpathway of pyrimidine deoxyribonucleoside salvage
PlantCyc:ARA_PWY-7193 pyrimidine ribonucleosides salvage I
PlantCyc:ARA_PWY-7199 pyrimidine deoxyribonucleosides salvage
PlantCyc:ARA_PWY-7196 superpathway of pyrimidine ribonucleosides salvage
Reactome:R-DDI-8956321 Nucleotide salvage
Reactome:R-MMU-1430728 Metabolism
Reactome:R-SCE-73614 Pyrimidine salvage
Reactome:R-SPO-1430728 Metabolism
Reactome:R-SPO-15869 Nucleotide metabolism
Reactome:R-DDI-15869 Nucleotide metabolism
Reactome:R-HSA-8956321 Nucleotide salvage
Reactome:R-MMU-8956321 Nucleotide salvage
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SPO-73614 Pyrimidine salvage
Reactome:R-HSA-73614 Pyrimidine salvage
Reactome:R-MMU-73614 Pyrimidine salvage
Reactome:R-SCE-15869 Nucleotide metabolism
Reactome:R-SPO-8956321 Nucleotide salvage
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-73614 Pyrimidine salvage
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-15869 Nucleotide metabolism
Reactome:R-MMU-15869 Nucleotide metabolism
Reactome:R-SCE-8956321 Nucleotide salvage
PathBank:SMP0000178 Dihydropyrimidinase Deficiency
PathBank:SMP0000469 Capecitabine Action Pathway
PathBank:SMP0012077 Pyrimidine Deoxyribonucleosides Salvage
PathBank:SMP0000046 Pyrimidine Metabolism
PathBank:SMP0000219 UMP Synthase Deficiency (Orotic Aciduria)
PathBank:SMP0000202 MNGIE (Mitochondrial Neurogastrointestinal Encephalopathy)
PathBank:SMP0000172 beta-Ureidopropionase Deficiency
PathBank:SMP0000607 Capecitabine Metabolism Pathway