EC: 3.5.2.9

5-oxoprolinase (ATP-hydrolysing) (5-oxo-L-proline amidohydrolase (ATP-hydrolysing))

enzyme lambda metabolic reaction experiment
- [5-oxoprolinase (ATP-hydrolysing)(Enzyme) wildtype]

2 H2O + ATP + 5_Oxo_L_proline -> L_Glutamate + ADP + Phosphate		 (( (V * A) ) / (Km + A)) buffer: 0.05 M Tris-HCl, 5 mM phosphocreatine, 10 mM MgCl2, 4 mM methionine sulfoximine, 0.15 M KCl, 4 U/ml creatine phosphokinase, 4 mM dithiothreitol
PH: 7.8
Temperature: 37
uniprot:P28273 [K+]

2 H2O + 5_Oxoproline + ATP -> ADP + Phosphate + L_Glutamate		 (( (Vmax * S) ) / (Km + S)) buffer: 50 mM Tris-HCl
PH: 8
Temperature: 37

Pathways

pathway id name
BioCyc:HUMAN_PWY-4041 γ-glutamyl cycle
BioCyc:META_PWY-4041 γ-glutamyl cycle
BioCyc:ARA_PWY-4041 γ-glutamyl cycle
BioCyc:MOUSE_PWY-4041 γ-glutamyl cycle
BioCyc:ARA_PWYQT-4470 γ-glutamyl cycle (plant pathway)
BioCyc:YEAST_PWY-4041 γ-glutamyl cycle
PlantCyc:ARA_PWYQT-4470 γ-glutamyl cycle (plant pathway)
PlantCyc:ARA_PWY-4041 γ-glutamyl cycle
PlantCyc:PLANT_PWY-4041 γ-glutamyl cycle
PlantCyc:PLANT_PWYQT-4470 γ-glutamyl cycle (plant pathway)
Reactome:R-DDI-211859 Biological oxidations
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-RNO-156590 Glutathione conjugation
Reactome:R-DDI-174403 Glutathione synthesis and recycling
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-RNO-211859 Biological oxidations
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-174403 Glutathione synthesis and recycling
Reactome:R-DDI-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-156590 Glutathione conjugation
Reactome:R-MMU-156590 Glutathione conjugation
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-174403 Glutathione synthesis and recycling
Reactome:R-SCE-156580 Phase II - Conjugation of compounds
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-156590 Glutathione conjugation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-174403 Glutathione synthesis and recycling
Reactome:R-MMU-174403 Glutathione synthesis and recycling
Reactome:R-RNO-156580 Phase II - Conjugation of compounds
Reactome:R-SCE-211859 Biological oxidations
Reactome:R-SCE-156590 Glutathione conjugation
WikiPathways:WP4518 Gamma-glutamyl cycle for the biosynthesis and degradation of glutathione, including diseases
PathBank:SMP0120480 gamma-Glutamyltransferase Deficiency
PathBank:SMP0120565 5-Oxoprolinase Deficiency
PathBank:SMP0000015 Glutathione Metabolism
PathBank:SMP0000500 5-Oxoprolinase Deficiency
PathBank:SMP0120485 Glutathione Synthetase Deficiency
PathBank:SMP0120566 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0000337 Glutathione Synthetase Deficiency
PathBank:SMP0000501 gamma-Glutamyltranspeptidase Deficiency
PathBank:SMP0087167 Glutathione Metabolism
PathBank:SMP0120446 5-Oxoprolinuria
PathBank:SMP0000143 5-Oxoprolinuria
PathBank:SMP0000183 gamma-Glutamyltransferase Deficiency
PathBank:SMP0063622 Glutathione Metabolism