EC: 3.1.3.76

lipid-phosphate phosphatase ((9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate phosphohydrolase)

enzyme lambda metabolic reaction experiment
uniprot:P34913 [Mg2+]

4_Nitrophenyl_phosphate + H2O -> p_Nitrophenol + Phosphate		 (( (Vmax * (S ^ n)) ) / (( (Km + S) ) ^ n)) buffer: 100 mM Sodium phosphate, 0.1 mg/ml BSA, 1 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + threo_10_Phosphonooxy_9_hydroxy_octadecanoic_acid -> Phosphate + threo_9,10_Dihydroxyoctadecanoate		 (( (Vmax * (S ^ n)) ) / (( (Km + S) ) ^ n)) buffer: 100 mM Sodium phosphate, 0.1 mg/ml BSA, 1 mM MgCl2
PH: 7.4
Temperature: 30
uniprot:P80299 [Mg2+]

H2O + 4_Nitrophenyl_phosphate -> p_Nitrophenol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 50 mM NaCl
PH: 7.8
Temperature: 37
uniprot:P80299 [Mg2+]

H2O + 4_Methylumbelliferyl_phosphate -> 4_Methylumbelliferone + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 50 mM NaCl
PH: 7.8
Temperature: 37
uniprot:P34913 [Mg2+]

1_Palmitoylglycerol_3_phosphate + H2O -> 1_Hexadecanoyl_sn_glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

H2O + 1_Oleoyl_lysophosphatidate -> Phosphate + sn_1_Monooleoylglycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

1_Stearoylglycerol_3_phosphate + H2O -> Phosphate + 1_Stearoyl_sn_glycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

H2O + Sphingosine_1_phosphate -> Sphingosine + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

1_Oleoyl_lysophosphatidate + H2O -> sn_1_Monooleoylglycerol + Phosphate		 (( (( (E * kcat) ) * (A ^ n)) ) / (( (S_half ^ n) ) + (A ^ n))) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + 1_Arachidonoyl_sn_glycerol_3_phosphate -> 1_Arachidonoylglycerol + Phosphate		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + 1_Stearoylglycerol_3_phosphate -> Phosphate + 1_Stearoyl_sn_glycerol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + N_Acetylsphingosine_1_phosphate -> Phosphate + N_Acetylsphingosine		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + Sphingosine_1_phosphate -> Sphingosine + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

1_Palmitoylglycerol_3_phosphate + H2O -> Phosphate + 1_Hexadecanoyl_sn_glycerol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + 1_Myristoylglycerol_3_phosphate -> Phosphate + 1_Myristoyl_sn_glycerol		 (( (( (E * kcat) ) * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 30
uniprot:P34913 [Mg2+]

H2O + 1_Palmitoylglycerol_3_phosphate -> 1_Hexadecanoyl_sn_glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

1_Arachidonoyl_sn_glycerol_3_phosphate + H2O -> Phosphate + 1_Arachidonoylglycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

1_Octadecyllysophosphatidic_cid + H2O -> 1_O_Octadecyl_sn_glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

H2O + Geranylgeranyl_diphosphate -> Geranylgeranyl_phosphate + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

H2O + 1_Stearoylglycerol_3_phosphate -> 1_Stearoyl_sn_glycerol + Phosphate		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37
uniprot:P34913 [Mg2+]

1_Arachidoylglycerol_3_phosphate + H2O -> Phosphate + 1_Arachidoyl_sn_glycerol		 (( (Vmax * A) ) / (Km + A)) buffer: 25 mM Bis Tris-HCl, 0.1 mg/ml BSA
PH: 7
Temperature: 37

Pathways

pathway id name
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-556833 Metabolism of lipids
Reactome:R-RNO-8978868 Fatty acid metabolism
Reactome:R-RNO-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-RNO-9018682 Biosynthesis of maresins
Reactome:R-SSC-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
Reactome:R-SSC-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-SSC-9018682 Biosynthesis of maresins
Reactome:R-HSA-556833 Metabolism of lipids
Reactome:R-HSA-8978868 Fatty acid metabolism
Reactome:R-MMU-8978868 Fatty acid metabolism
Reactome:R-MMU-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-MMU-9018682 Biosynthesis of maresins
Reactome:R-RNO-2142753 Arachidonate metabolism
Reactome:R-RNO-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
Reactome:R-RNO-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-SSC-1430728 Metabolism
Reactome:R-SSC-556833 Metabolism of lipids
Reactome:R-SSC-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-HSA-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-RNO-1430728 Metabolism
Reactome:R-RNO-556833 Metabolism of lipids
Reactome:R-SSC-2142753 Arachidonate metabolism
Reactome:R-HSA-1430728 Metabolism
Reactome:R-HSA-2142753 Arachidonate metabolism
Reactome:R-HSA-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
Reactome:R-HSA-9018677 Biosynthesis of DHA-derived SPMs
Reactome:R-HSA-9018682 Biosynthesis of maresins
Reactome:R-MMU-2142753 Arachidonate metabolism
Reactome:R-MMU-2142670 Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET)
Reactome:R-MMU-9018678 Biosynthesis of specialized proresolving mediators (SPMs)
Reactome:R-SSC-8978868 Fatty acid metabolism
WikiPathways:WP5064 7-oxo-C and 7-beta-HC pathways
WikiPathways:WP4545 Oxysterols derived from cholesterol
PathBank:SMP0120730 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000083 Acetylsalicylic Acid Action Pathway
PathBank:SMP0000087 Rofecoxib Action Pathway
PathBank:SMP0000094 Sulindac Action Pathway
PathBank:SMP0000104 Indomethacin Action Pathway
PathBank:SMP0000120 Naproxen Action Pathway
PathBank:SMP0000106 Meloxicam Action Pathway
PathBank:SMP0000693 Antrafenine Action Pathway
PathBank:SMP0000697 Flurbiprofen Action Pathway
PathBank:SMP0000701 Phenylbutazone Action Pathway
PathBank:SMP0000705 Tiaprofenic Acid Action Pathway
PathBank:SMP0000709 Salicylic Acid Action Pathway
PathBank:SMP0000075 Arachidonic Acid Metabolism
PathBank:SMP0000086 Ibuprofen Action Pathway
PathBank:SMP0000093 Diclofenac Action Pathway
PathBank:SMP0000098 Ketorolac Action Pathway
PathBank:SMP0000102 Bromfenac Action Pathway
PathBank:SMP0000114 Nabumetone Action Pathway
PathBank:SMP0000692 Antipyrine Action Pathway
PathBank:SMP0000696 Fenoprofen Action Pathway
PathBank:SMP0000700 Lornoxicam Action Pathway
PathBank:SMP0000704 Tolmetin Action Pathway
PathBank:SMP0000708 Salicylate-Sodium Action Pathway
PathBank:SMP0063595 Arachidonic Acid Metabolism
PathBank:SMP0000077 Piroxicam Action Pathway
PathBank:SMP0000084 Etodolac Action Pathway
PathBank:SMP0000109 Mefenamic Acid Action Pathway
PathBank:SMP0000289 Diflunisal Action Pathway
PathBank:SMP0000116 Valdecoxib Action Pathway
PathBank:SMP0000694 Carprofen Action Pathway
PathBank:SMP0000698 Magnesium Salicylate Action Pathway
PathBank:SMP0000702 Nepafenac Action Pathway
PathBank:SMP0000706 Tenoxicam Action Pathway
PathBank:SMP0000710 Acetaminophen Action Pathway
PathBank:SMP0120510 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000353 Leukotriene C4 Synthesis Deficiency
PathBank:SMP0000085 Ketoprofen Action Pathway
PathBank:SMP0000096 Celecoxib Action Pathway
PathBank:SMP0000101 Suprofen Action Pathway
PathBank:SMP0000113 Oxaprozin Action Pathway
PathBank:SMP0000695 Etoricoxib Action Pathway
PathBank:SMP0000699 Lumiracoxib Action Pathway
PathBank:SMP0000703 Trisalicylate-Choline Action Pathway
PathBank:SMP0000707 Salsalate Action Pathway
PathBank:SMP0087315 Arachidonic Acid Metabolism