EC: 2.7.7.9

UTP-glucose-1-phosphate uridylyltransferase (UTP:alpha-D-glucose-1-phosphate uridylyltransferase)

enzyme lambda metabolic reaction experiment
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose
(( (( (UGPVmax * G1P) ) * UTP) ) / (( (UGPKg1p * UGPKutp) ) * (( (( (( (( (( (UGPKiutp / UGPKutp) ) + (G1P / UGPKg1p)) ) + (( (UGPKiutp * UDG) ) / (UGPKiudg * UGPKutp))) ) + (( (G1P * UDG) ) / (UGPKg1p * UGPKiudg))) ) + (UTP / UGPKutp)) ) + (( (G1P * UTP) ) / (UGPKg1p * UGPKutp))))) buffer: *
PH: 7
Temperature: 36
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]

UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform I]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform II]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform I]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 2 mM NAD+, 0.25 U/ml UDP-glucose dehydrogenase
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform LacZ fusion protein]

UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform LacZ fusion protein]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform II]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * S) ) / (S + Km)) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]

UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]

UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]

UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]

UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]

UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) buffer: *
PH: 7
Temperature: 36
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: *
PH: 7
Temperature: 36
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]

Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]

UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

dTTP + D_Glucose_1_phosphate -> dTDPglucose + Diphosphate
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37
uniprot:P19595 [Mg2+]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate
(( (Vmax * B) ) / (Km + B)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P19595 [Mg2+]

D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 20 mM Tris-HCl
PH: 8
Temperature: 30
uniprot:P19595 [Mg2+]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 50 mM Tris-HCl, 0.5 mM mercaptoethanol, 10 mM NaF, 0.02 mM glucose-1,6-bisphosphate, 0.3 mM NADP, 1.35 U/ml phosphoglucomutase, 1.35 U/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
uniprot:P19595 [Mg2+]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * B) ) / (Km + B)) buffer: 50 mM Tris-HCl, 0.5 mM mercaptoethanol, 10 mM NaF, 0.02 mM glucose-1,6-bisphosphate, 0.3 mM NADP, 1.35 U/ml phosphoglucomutase, 1.35 U/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30
uniprot:P19595 [Mg2+]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: 80 mM Glycylglycine, 1 U/ml PGM, 1 U/ml Glucose-6-P dehydrogenase, 20 mM Cys, 0.02 mM Glucose-1,6-diphospahte, 0.6 mM NADP
PH: 8.5
Temperature: 37
uniprot:P19595 [Mg2+]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * B) ) / (Km + B)) buffer: 80 mM Glycylglycine, 1 U/ml PGM, 1 U/ml Glucose-6-P dehydrogenase, 20 mM Cys, 0.02 mM Glucose-1,6-diphospahte, 0.6 mM NADP
PH: 8.5
Temperature: 37
uniprot:P19595 [Mg2+]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * B) ) / (Km + B)) buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37
uniprot:P19595 [Mg2+]

alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate
(( (Vmax * B) ) / (Km + B)) buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37
uniprot:P19595 [Mg2+]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37
uniprot:P19595 [Mg2+]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-67]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-67]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-37]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-32]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-32]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-101]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-101]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-8]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-8]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]

UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]

UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]

D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S) ) / (Km + S)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-4 (deletion of aa 428-431)]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-8 (deletion of aa 426-433)]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-4 (deletion of aa 428-431)]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-8 (deletion of aa 426-433)]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Y192A]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Y192A]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K183A]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K183A]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * B) ) / (Km + B)) buffer: Step1: 80 mM glycylglycine, 8 mM MgCl2; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 1 U/ml phosphoglucomutase, 100-500 µlof supernatant from step1
PH: 7.5
Temperature: 37
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate
(( (Vmax * B) ) / (Km + B)) buffer: Step1: 80 mM glycylglycine, 5 mM MgCl2; Step2: 80 mM glycylglycine, 20 mM Cys, 0.6 mM NAD+, 0.75 U/ml Glc-6-P-dehydrogenase, 1 U/ml phosphoglucomutase, an aliqot of step 1
PH: 8
Temperature: 37
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km + A)) buffer: Step1: 80 mM glycylglycine, 8 mM MgCl2; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 1 U/ml phosphoglucomutase, 100-500 µlof supernatant from step1
PH: 7.5
Temperature: 37
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP
(( (Vmax * A) ) / (Km + A)) buffer: Step1: 80 mM glycylglycine, 5 mM MgCl2; Step2: 80 mM glycylglycine, 20 mM Cys, 0.6 mM NAD+, 0.75 U/ml Glc-6-P-dehydrogenase, 1 U/ml phosphoglucomutase, an aliqot of step 1
PH: 8
Temperature: 37
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-37]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-21]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K405A]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-21]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K405A]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K260A]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K260A]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K322A]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S2) ) / (Km + S2)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K322A]

Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP
(( (Vmax * S1) ) / (Km + S1)) buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S) ) / (Km + S)) buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S) ) / (Km + S)) buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37
uniprot:P19595 [Glycerate 3-phosphate]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S) ) / (Km + S)) buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37
uniprot:P19595 [Glycerate 3-phosphate]

UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP
(( (Vmax * S) ) / (Km + S)) buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37
uniprot:F4IY62 [Mg2+]

alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose
(( (Vmax * A) ) / (Km_A + A)) buffer: 50 mM Tris-HCl
PH: 7.8
Temperature: 30
uniprot:F4IY62 [Mg2+]

UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose
(( (Vmax * B) ) / (Km_B + B)) buffer: 50 mM Tris-HCl
PH: 7.8
Temperature: 30
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose
(( (Vmax * A) ) / (Km + A)) buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate
(( (Vmax * A) ) / (Km + A)) buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose
(( (Vmax * A) ) / (Km + A)) buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose
(( (Vmax * A) ) / (Km + A)) buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate
(( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate
(( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate
(( (( (kcat * A) ) * E) ) / (Km + A)) buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]

UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate
(( (( (kcat * B) ) * E) ) / (Km + B)) buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25

Pathways

pathway id name
BioCyc:META_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:META_PWY-7328 superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis
BioCyc:YEAST_PWY-7343 UDP-glucose biosynthesis
BioCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
BioCyc:ECOL199310_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOL316407_PWY-7343 UDP-glucose biosynthesis
BioCyc:ECOL316407_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:ECOL316407_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:META_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:META_UDPNAGSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis I
BioCyc:META_PWY-8013 UDP-N-acetyl-D-galactosamine biosynthesis III
BioCyc:META_PWY-7343 UDP-α-D-glucose biosynthesis I
BioCyc:META_PWY-7345 superpathway of anaerobic sucrose degradation
BioCyc:YEAST_PWY3O-1565 dolichyl glucosyl phosphate biosynthesis
BioCyc:ECOL199310_PWY-6415 L-ascorbate biosynthesis V
BioCyc:MOUSE_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:HUMAN_PWY-7343 UDP-α-D-glucose biosynthesis I
BioCyc:META_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:META_PWY-7238 sucrose biosynthesis II
BioCyc:META_PWY-3801 sucrose degradation II (sucrose synthase)
BioCyc:ECO_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECO_PWY-7343 UDP-α-D-glucose biosynthesis I
BioCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
BioCyc:ARA_PWY-6527 stachyose degradation
BioCyc:ECOO157_COLANSYN-PWY colanic acid building blocks biosynthesis
BioCyc:ECOO157_PWY-7343 UDP-glucose biosynthesis
BioCyc:ECOL199310_PWY3DJ-35471 L-ascorbate biosynthesis IV
BioCyc:MOUSE_GALACTMETAB-PWY UDP-galactose biosynthesis (salvage pathway from galactose using UDP-glucose)
BioCyc:MOUSE_PWY3DJ-35471 L-ascorbate biosynthesis VI
BioCyc:MOUSE_P61-PWY UDP-glucose conversion
BioCyc:GCF_000013425_PWY-7343 UDP-α-D-glucose biosynthesis I
BioCyc:ECO_GALACTMETAB-PWY galactose degradation I (Leloir pathway)
BioCyc:ARA_PWY-7238 sucrose biosynthesis II
BioCyc:ARA_PWY-7343 UDP-glucose biosynthesis
BioCyc:ARA_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
BioCyc:ECOO157_PWY3DJ-35471 L-ascorbate biosynthesis IV
BioCyc:ECOO157_PWY-6415 L-ascorbate biosynthesis V
BioCyc:ECOO157_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
BioCyc:ECOL199310_PWY-7343 UDP-glucose biosynthesis
PathBank:SMP0000841 Galactose Metabolism
PathBank:SMP0000903 Galactose Degradation/Leloir Pathway
PathBank:SMP0000914 Amino Sugar and Nucleotide Sugar Metabolism III
PathBank:SMP0000967 Colanic Acid Building Blocks Biosynthesis
PlantCyc:ARA_PWY-6527 stachyose degradation
PlantCyc:ARA_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-7343 UDP-α-D-glucose biosynthesis I
PlantCyc:PLANT_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
PlantCyc:PLANT_PWY-7238 sucrose biosynthesis II
PlantCyc:PLANT_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:ARA_PWY-7343 UDP-α-D-glucose biosynthesis I
PlantCyc:ARA_UDPNACETYLGALSYN-PWY UDP-N-acetyl-D-glucosamine biosynthesis II
PlantCyc:ARA_PWY-7238 sucrose biosynthesis II
PlantCyc:ARA_PWY-3801 sucrose degradation II (sucrose synthase)
PlantCyc:PLANT_PWY-7345 superpathway of anaerobic sucrose degradation
PlantCyc:PLANT_PWY-3801 sucrose degradation II (sucrose synthase)
PlantCyc:ARA_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
PlantCyc:ARA_SUCSYN-PWY sucrose biosynthesis I (from photosynthesis)
PlantCyc:PLANT_PWYQT-4466 superpathway of sucrose and starch metabolism I (non-photosynthetic tissue)
Reactome:R-BTA-1430728 Metabolism
Reactome:R-BTA-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-DDI-8982491 Glycogen metabolism
Reactome:R-DDI-211859 Biological oxidations
Reactome:R-HSA-8982491 Glycogen metabolism
Reactome:R-HSA-211859 Biological oxidations
Reactome:R-HSA-156588 Glucuronidation
Reactome:R-MMU-1430728 Metabolism
Reactome:R-MMU-211859 Biological oxidations
Reactome:R-MMU-156588 Glucuronidation
Reactome:R-SCE-71387 Carbohydrate metabolism
Reactome:R-SPO-1430728 Metabolism
Reactome:R-BTA-3322077 Glycogen synthesis
Reactome:R-BTA-211859 Biological oxidations
Reactome:R-BTA-156588 Glucuronidation
Reactome:R-DDI-71387 Carbohydrate metabolism
Reactome:R-DDI-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-HSA-71387 Carbohydrate metabolism
Reactome:R-HSA-156580 Phase II - Conjugation of compounds
Reactome:R-MMU-3322077 Glycogen synthesis
Reactome:R-MMU-156580 Phase II - Conjugation of compounds
Reactome:R-SCE-1430728 Metabolism
Reactome:R-SCE-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-SPO-3322077 Glycogen synthesis
Reactome:R-SPO-211859 Biological oxidations
Reactome:R-SPO-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-BTA-71387 Carbohydrate metabolism
Reactome:R-DDI-3322077 Glycogen synthesis
Reactome:R-DDI-156580 Phase II - Conjugation of compounds
Reactome:R-HSA-3322077 Glycogen synthesis
Reactome:R-HSA-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-MMU-71387 Carbohydrate metabolism
Reactome:R-MMU-173599 Formation of the active cofactor, UDP-glucuronate
Reactome:R-SCE-8982491 Glycogen metabolism
Reactome:R-SCE-156580 Phase II - Conjugation of compounds
Reactome:R-SPO-71387 Carbohydrate metabolism
Reactome:R-SPO-156580 Phase II - Conjugation of compounds
Reactome:R-BTA-8982491 Glycogen metabolism
Reactome:R-BTA-156580 Phase II - Conjugation of compounds
Reactome:R-DDI-1430728 Metabolism
Reactome:R-DDI-156588 Glucuronidation
Reactome:R-HSA-1430728 Metabolism
Reactome:R-MMU-8982491 Glycogen metabolism
Reactome:R-SCE-3322077 Glycogen synthesis
Reactome:R-SCE-211859 Biological oxidations
Reactome:R-SCE-156588 Glucuronidation
Reactome:R-SPO-8982491 Glycogen metabolism
Reactome:R-SPO-156588 Glucuronidation
WikiPathways:WP2623 Sucrose metabolism
WikiPathways:WP5312 Metabolic pathways of fibroblasts
PathBank:SMP0000444 Lactose Synthesis
PathBank:SMP0000496 Galactosemia III
PathBank:SMP0000552 Glycogen Synthetase Deficiency
PathBank:SMP0000556 Mucopolysaccharidosis VII. Sly Syndrome
PathBank:SMP0000580 GLUT-1 Deficiency Syndrome
PathBank:SMP0002319 Leloir Pathway
PathBank:SMP0002331 Amino Sugar and Nucleotide Sugar Metabolism
PathBank:SMP0121303 Amino Sugar and Nucleotide Sugar Metabolism III
PathBank:SMP0000043 Galactose Metabolism
PathBank:SMP0000182 Galactosemia
PathBank:SMP0000495 Galactosemia II (GALK)
PathBank:SMP0000555 Glycogenosis, Type VI. Hers Disease
PathBank:SMP0000579 Congenital Disorder of Glycosylation CDG-IId
PathBank:SMP0000010 Nucleotide Sugars Metabolism
PathBank:SMP0000553 Glycogenosis, Type III. Cori Disease, Debrancher Glycogenosis
PathBank:SMP0000557 Sucrase-Isomaltase Deficiency
PathBank:SMP0000058 Starch and Sucrose Metabolism
PathBank:SMP0000554 Glycogenosis, Type IV. Amylopectinosis, Anderson Disease
PathBank:SMP0002380 Starch and Sucrose Metabolism