- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose |
(( (( (UGPVmax * G1P) ) * UTP) ) / (( (UGPKg1p * UGPKutp) ) * (( (( (( (( (( (UGPKiutp / UGPKutp) ) + (G1P / UGPKg1p)) ) + (( (UGPKiutp * UDG) ) / (UGPKiudg * UGPKutp))) ) + (( (G1P * UDG) ) / (UGPKg1p * UGPKiudg))) ) + (UTP / UGPKutp)) ) + (( (G1P * UTP) ) / (UGPKg1p * UGPKutp))))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]
UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (( (Km * (1 + (I / Ki))) ) + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform I]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform II]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform I]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 2 mM NAD+, 0.25 U/ml UDP-glucose dehydrogenase
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform LacZ fusion protein]
UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate |
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform LacZ fusion protein]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (( (Vmax * A) ) * B) ) / (( (( (( (A * B) ) + (Ka * B)) ) + (Kb * A)) ) + (Kia * Kb))) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype muscle isoform II]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * S) ) / (S + Km)) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+, 5 U/ml Phosphoglucomutase, 2 µM Glucose 1,6-bisphosphate, 5 U/ml Glucose 6-phosphate dehydrogenase, 1 mM NADP+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype liver isoform]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M triethanolamine/HCl, 1 mM dithiothreitol, 1 mM Mg2+
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]
UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]
UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]
UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]
UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R445H]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R422Q]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant R389H]
UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant W218S]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant H266R]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * (S ^ n)) ) / (( (S_half ^ n) ) + (S ^ n))) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UTP + alpha_D_Glucose_1_phosphate -> UDP_D_glucose + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: *
PH: 7
Temperature: 36 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype N-terminal lacZ(42aa)-fusion]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]
Diphosphate + UDP_D_glucose -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]
UDP_D_glucose + Diphosphate -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:Q16851 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant C123S]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 0.1 M Triethanolamine/HCl, 1 mM DTT, 1 mM MgCl2
PH: 8
Temperature: 25 |
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37 |
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37 |
uniprot:P0AEP3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
dTTP + D_Glucose_1_phosphate -> dTDPglucose + Diphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM Tris-HCl, 10 mM MgCl2, 2 mM KH2PO4, 1 U/ml inorganic pyrophosphatase
PH: 7.8
Temperature: 37 |
uniprot:P19595 [Mg2+]
UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 20 mM Tris-HCl
PH: 8
Temperature: 30 |
uniprot:P19595 [Mg2+]
D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 20 mM Tris-HCl
PH: 8
Temperature: 30 |
uniprot:P19595 [Mg2+]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 0.5 mM mercaptoethanol, 10 mM NaF, 0.02 mM glucose-1,6-bisphosphate, 0.3 mM NADP, 1.35 U/ml phosphoglucomutase, 1.35 U/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P19595 [Mg2+]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 50 mM Tris-HCl, 0.5 mM mercaptoethanol, 10 mM NaF, 0.02 mM glucose-1,6-bisphosphate, 0.3 mM NADP, 1.35 U/ml phosphoglucomutase, 1.35 U/ml glucose-6-P dehydrogenase
PH: 8
Temperature: 30 |
uniprot:P19595 [Mg2+]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 80 mM Glycylglycine, 1 U/ml PGM, 1 U/ml Glucose-6-P dehydrogenase, 20 mM Cys, 0.02 mM Glucose-1,6-diphospahte, 0.6 mM NADP
PH: 8.5
Temperature: 37 |
uniprot:P19595 [Mg2+]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: 80 mM Glycylglycine, 1 U/ml PGM, 1 U/ml Glucose-6-P dehydrogenase, 20 mM Cys, 0.02 mM Glucose-1,6-diphospahte, 0.6 mM NADP
PH: 8.5
Temperature: 37 |
uniprot:P19595 [Mg2+]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37 |
uniprot:P19595 [Mg2+]
alpha_D_Glucose_1_phosphate + UTP -> UDP_D_glucose + Diphosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37 |
uniprot:P19595 [Mg2+]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37 |
uniprot:P19595 [Mg2+]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: Step1: 80 mM glycylglycine; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 100-500 µl of supernatant from step1
PH: 8.5
Temperature: 37 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-67]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-67]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-37]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-32]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-32]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-101]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-101]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-8]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ccut-8]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]
UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36 |
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]
UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36 |
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]
UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]
D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2
PH: 7.5
Temperature: 36 |
uniprot:F4KGY8 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP2, his-tagged]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36 |
uniprot:Q9M9P3 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype UGP1, his-tagged]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADPH, 5 units/ml PGM, 5 units/ml GDH
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-4 (deletion of aa 428-431)]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-8 (deletion of aa 426-433)]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-4 (deletion of aa 428-431)]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Del-I-8 (deletion of aa 426-433)]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Y192A]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Y192A]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K183A]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K183A]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: Step1: 80 mM glycylglycine, 8 mM MgCl2; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 1 U/ml phosphoglucomutase, 100-500 µlof supernatant from step1
PH: 7.5
Temperature: 37 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDP_D_glucose + Diphosphate -> UTP + alpha_D_Glucose_1_phosphate |
(( (Vmax * B) ) / (Km + B)) |
|
buffer: Step1: 80 mM glycylglycine, 5 mM MgCl2; Step2: 80 mM glycylglycine, 20 mM Cys, 0.6 mM NAD+, 0.75 U/ml Glc-6-P-dehydrogenase, 1 U/ml phosphoglucomutase, an aliqot of step 1
PH: 8
Temperature: 37 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: Step1: 80 mM glycylglycine, 8 mM MgCl2; Step2: 80 mM glycylglycine, 3 mM NAD+, 0.05 U/ml UDP-Glc-dehydrogenase, 1 U/ml phosphoglucomutase, 100-500 µlof supernatant from step1
PH: 7.5
Temperature: 37 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDP_D_glucose -> alpha_D_Glucose_1_phosphate + UTP |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: Step1: 80 mM glycylglycine, 5 mM MgCl2; Step2: 80 mM glycylglycine, 20 mM Cys, 0.6 mM NAD+, 0.75 U/ml Glc-6-P-dehydrogenase, 1 U/ml phosphoglucomutase, an aliqot of step 1
PH: 8
Temperature: 37 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-37]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-21]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K405A]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant Ncut-21]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K405A]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K260A]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K260A]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K322A]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S2) ) / (Km + S2)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:Q43772 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) mutant K322A]
Diphosphate + UDPglucose -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S1) ) / (Km + S1)) |
|
buffer: 100 mM Hepes, 5 mM MgCl2, 0.3 mM NADP, 5 U/ml phosphoglucomutase, 5 U/ml glucose-6-P dehydrogenase
PH: 7.5
Temperature: 36 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37 |
uniprot:P19595 [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37 |
uniprot:P19595 [Glycerate 3-phosphate]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37 |
uniprot:P19595 [Glycerate 3-phosphate]
UDPglucose + Diphosphate -> D_Glucose_1_phosphate + UTP |
(( (Vmax * S) ) / (Km + S)) |
|
buffer: 80 mM Glycylglycine, 5 mM MgCl2, 10 mM NaF
PH: 8
Temperature: 37 |
uniprot:F4IY62 [Mg2+]
alpha_D_Glucose_1_phosphate + UTP -> Diphosphate + UDP_D_glucose |
(( (Vmax * A) ) / (Km_A + A)) |
|
buffer: 50 mM Tris-HCl
PH: 7.8
Temperature: 30 |
uniprot:F4IY62 [Mg2+]
UTP + alpha_D_Glucose_1_phosphate -> Diphosphate + UDP_D_glucose |
(( (Vmax * B) ) / (Km_B + B)) |
|
buffer: 50 mM Tris-HCl
PH: 7.8
Temperature: 30 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UTP + D_Glucose_1_phosphate -> Diphosphate + UDPglucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UTP -> Diphosphate + UDPglucose |
(( (Vmax * A) ) / (Km + A)) |
|
buffer: 60 mM Bicine, 5 mM mercaptoethanol, 5 mM MgCl2
PH: 7.8
Temperature: 38 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UTP + D_Glucose_1_phosphate -> UDPglucose + Diphosphate |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
D_Glucose_1_phosphate + UTP -> UDPglucose + Diphosphate |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
Diphosphate + UDPglucose -> UTP + D_Glucose_1_phosphate |
(( (( (kcat * A) ) * E) ) / (Km + A)) |
|
buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25 |
- [UTP---glucose-1-phosphate uridylyltransferase(Enzyme) wildtype]
UDPglucose + Diphosphate -> UTP + D_Glucose_1_phosphate |
(( (( (kcat * B) ) * E) ) / (Km + B)) |
|
buffer: 50 mM Tris-HCl, 3 mM MgCl2
PH: 7.5
Temperature: 25 |